FastQCFastQC Report
Thu 26 May 2016
SRR937009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706324
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138010.8088147385842314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100770.5905677936898268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73520.43086776016747114No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.13672667090189203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.11000255520053637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54200.053.300181
GTATCAA102200.043.1441081
ATCAACG134150.032.571923
TCAACGC135800.032.2111364
TATCAAC136400.032.0732042
CGCGAGA300.003953579331.66413554-55
CAACGCA139000.031.4732745
AACGCAG142100.030.7532396
ACGCAGA162300.026.837867
CGCAGAG161750.026.8116518
GTACATG95350.026.1615221
GCAGAGT171000.025.0279799
ACATGGG96250.024.3765583
TACATGG102250.024.2031292
CGCAGTA1000.001653896123.750192
GAGTACT103950.022.79933712-13
CATGGGG31800.022.5525684
AGTACTT110850.022.38704712-13
GGACCGC1302.9421577E-421.9232526
GTACTTT109450.021.50239414-15
Produced by FastQC (version 0.11.5)