FastQCFastQC Report
Thu 26 May 2016
SRR937008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726734
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122030.706709892780243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84930.4918534064887817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.34707140764008815No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.1113084007148756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50550.053.9870071
GTATCAA96600.042.8686261
ATCAACG125350.033.1160743
TCAACGC126850.032.761964
TATCAAC128250.032.3301732
CAACGCA129250.032.1149645
AACGCAG132650.031.2863676
ACGCAGA149450.027.5411437
CGCAGAG149800.027.381618
GTACATG98550.026.1481631
GCAGAGT156600.025.3117229
ACATGGG99100.024.4706463
TACATGG103050.024.3632282
GTATAGG6550.023.953621
GAGTACT88950.023.48608812-13
CATGGGA62550.022.805674
GTACTTT95250.021.93172514-15
AGAGTAC145600.021.1201310-11
CAGAGTA155050.020.96708310-11
GATATAG11800.020.951731
Produced by FastQC (version 0.11.5)