##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937008_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1726734 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74696392148414 33.0 31.0 34.0 30.0 34.0 2 31.83610909381526 33.0 31.0 34.0 30.0 34.0 3 31.84202951931218 33.0 31.0 34.0 30.0 34.0 4 35.462987350686326 37.0 35.0 37.0 33.0 37.0 5 35.47142814121921 37.0 35.0 37.0 33.0 37.0 6 35.548021293378135 37.0 35.0 37.0 33.0 37.0 7 35.49111791393463 37.0 35.0 37.0 33.0 37.0 8 35.453310701011276 37.0 35.0 37.0 33.0 37.0 9 37.06281743453248 39.0 37.0 39.0 33.0 39.0 10-11 36.93358791800011 39.0 37.0 39.0 33.0 39.0 12-13 36.85518933431554 39.0 37.0 39.0 33.0 39.0 14-15 37.967984646158584 40.0 38.0 41.0 33.0 41.0 16-17 37.909151322670425 40.0 38.0 41.0 33.0 41.0 18-19 37.914395905796724 40.0 38.0 41.0 33.0 41.0 20-21 37.8690009578777 40.0 38.0 41.0 32.5 41.0 22-23 37.75786195210148 40.0 37.0 41.0 32.0 41.0 24-25 37.71215311680896 40.0 37.0 41.0 32.0 41.0 26-27 37.728642628221834 40.0 37.0 41.0 32.0 41.0 28-29 37.655052254719024 40.0 37.0 41.0 32.0 41.0 30-31 37.46054980095371 40.0 37.0 41.0 32.0 41.0 32-33 37.245264759945655 40.0 36.0 41.0 31.5 41.0 34-35 37.07399952743155 39.0 36.0 41.0 31.0 41.0 36-37 36.881378660523275 39.0 36.0 41.0 31.0 41.0 38-39 36.723842815396 39.0 35.0 41.0 30.0 41.0 40-41 36.522836464678406 39.0 35.0 41.0 30.0 41.0 42-43 36.272561378880596 39.0 35.0 41.0 30.0 41.0 44-45 35.96648412552252 39.0 35.0 40.5 29.5 41.0 46-47 35.54960752495752 39.0 35.0 40.0 27.5 41.0 48-49 35.24310808729081 38.5 35.0 40.0 26.0 41.0 50-51 34.5200711285004 38.0 34.0 39.5 25.0 40.5 52-53 34.69833396458285 38.0 34.5 40.0 25.5 40.5 54-55 35.08071248959018 39.0 35.0 40.5 26.0 41.0 56-57 34.98210552407029 39.0 35.0 41.0 25.5 41.0 58-59 34.775867620606306 38.5 34.5 40.0 25.0 41.0 60-61 34.51432994311805 38.0 34.0 40.0 24.5 41.0 62-63 34.21607641941376 38.0 34.0 40.0 23.0 41.0 64-65 33.89639197467589 37.0 34.0 40.0 22.5 41.0 66-67 33.541258815775905 37.0 33.5 39.5 22.0 41.0 68-69 33.1580231813354 36.0 33.0 39.0 21.5 41.0 70-71 32.75693650556484 36.0 33.0 39.0 20.0 40.5 72-73 32.379408467082946 35.0 32.0 38.5 18.5 40.0 74-75 31.98736806016445 35.0 32.0 37.5 18.0 39.5 76-77 31.540063785157415 35.0 31.5 37.0 14.5 39.0 78-79 31.083445394600442 35.0 31.0 36.5 9.5 39.0 80-81 30.66317278747045 35.0 31.0 36.0 7.0 38.5 82-83 30.264254946042644 34.5 30.5 35.5 4.5 37.0 84-85 29.891773718476614 34.0 30.0 35.0 2.0 37.0 86-87 29.554171053561234 34.0 30.0 35.0 2.0 36.0 88-89 29.20360055457297 34.0 29.5 35.0 2.0 36.0 90-91 28.85265449107969 34.0 29.0 35.0 2.0 36.0 92-93 28.493157023606415 34.0 29.0 35.0 2.0 35.0 94-95 28.14111293343387 34.0 29.0 35.0 2.0 35.0 96-97 27.676472461884693 34.0 28.0 35.0 2.0 35.0 98-99 27.137564037078093 34.0 27.0 35.0 2.0 35.0 100-101 24.940144515599968 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23524158581422938 1101 2 0.28791729174112035 1101 3 0.32684923618858974 1101 4 0.3106762394347271 1101 5 0.23967787894444115 1101 6 0.22926902163204232 1101 7 0.2748938626519717 1101 8 0.3085517850969737 1101 9 0.29587915345300075 1101 10-11 0.34856292197365235 1101 12-13 0.4754904946965297 1101 14-15 0.6204869063144614 1101 16-17 0.6182399152084272 1101 18-19 0.49698703066282235 1101 20-21 0.4082438477202075 1101 22-23 0.5270750639059401 1101 24-25 0.5139719477390443 1101 26-27 0.5506280264852492 1101 28-29 0.48159202885687336 1101 30-31 0.5973911068958699 1101 32-33 0.46224372141062986 1101 34-35 0.5769131858670136 1101 36-37 0.5516048373890854 1101 38-39 0.5349783569204263 1101 40-41 0.46286619345367797 1101 42-43 0.5175085798352725 1101 44-45 0.5646572237909169 1101 46-47 0.5389221839542984 1101 48-49 0.8418024268500375 1101 50-51 0.8668395885701798 1101 52-53 0.7853968740432435 1101 54-55 0.9049160646981136 1101 56-57 0.8915431018819788 1101 58-59 0.9556038326024421 1101 60-61 0.9091165224904714 1101 62-63 0.8346672215502267 1101 64-65 0.8274166832999512 1101 66-67 0.8131515701088361 1101 68-69 0.6516407389461705 1101 70-71 0.5419000506576381 1101 72-73 0.6093802024547017 1101 74-75 0.7114852096340698 1101 76-77 0.7398115741085434 1101 78-79 0.7730536353108732 1101 80-81 0.8954018523080691 1101 82-83 0.7420740494046143 1101 84-85 0.6813660216677953 1101 86-87 0.773468964871256 1101 88-89 0.6806272972698295 1101 90-91 0.7735403542360544 1101 92-93 0.8726830160530916 1101 94-95 0.9332920295412173 1101 96-97 0.830542702033302 1101 98-99 1.0630086675502923 1101 100-101 1.4528687348895204 1102 1 0.2802314871982823 1102 2 0.2209804881484807 1102 3 0.18062606812291193 1102 4 0.1989936158622143 1102 5 0.22903436639114716 1102 6 0.18418935477289722 1102 7 0.10818676790148629 1102 8 0.0895880720338269 1102 9 0.20648786927718987 1102 10-11 0.14414298092636812 1102 12-13 0.06360741170632878 1102 14-15 0.07497354109253962 1102 16-17 0.2265602417933863 1102 18-19 0.1550559634874844 1102 20-21 0.15149920780331882 1102 22-23 0.13445020542523878 1102 24-25 0.080536325470149 1102 26-27 0.14580842735364996 1102 28-29 -0.0944695865686711 1102 30-31 -0.15235699504341937 1102 32-33 -0.07723631963942523 1102 34-35 0.03608590623998964 1102 36-37 0.06269667301740611 1102 38-39 0.0338876811830815 1102 40-41 -0.0719578939516694 1102 42-43 -0.04073610168639874 1102 44-45 -0.03115342652969133 1102 46-47 -0.04772381480007937 1102 48-49 -0.043308481420233136 1102 50-51 -0.1802463114421755 1102 52-53 0.14491390552924344 1102 54-55 -0.08285427184677019 1102 56-57 0.004228148618018679 1102 58-59 -0.18443018246101417 1102 60-61 -0.17856669615653686 1102 62-63 -0.14868594759970222 1102 64-65 0.10862745818275954 1102 66-67 -0.32876109010110355 1102 68-69 -0.14983938279195286 1102 70-71 -0.20117325697317767 1102 72-73 -0.18101274843400006 1102 74-75 0.08087984740768661 1102 76-77 0.07671400962743746 1102 78-79 -0.08251901042564569 1102 80-81 0.06175265881051928 1102 82-83 0.12882153861888668 1102 84-85 0.05121541234681004 1102 86-87 0.252490193397513 1102 88-89 0.19266053571063324 1102 90-91 -0.014856953808290996 1102 92-93 0.062042277152112746 1102 94-95 0.034697766967116905 1102 96-97 -0.11617253716899612 1102 98-99 0.049239879207700454 1102 100-101 0.09549510627148194 1103 1 0.07710602299887626 1103 2 0.24586260070780597 1103 3 0.18213250724095076 1103 4 0.20990031703202305 1103 5 0.051103468971405164 1103 6 0.06357434242327287 1103 7 0.10278335876983391 1103 8 0.1144466224716183 1103 9 0.031210783850156076 1103 10-11 0.15313116196131915 1103 12-13 -0.0260170080764226 1103 14-15 0.2026515554818218 1103 16-17 0.16521139229080006 1103 18-19 0.08268388275750738 1103 20-21 0.0656124576038124 1103 22-23 0.12226679582234112 1103 24-25 0.05724926609462244 1103 26-27 0.2009670977533773 1103 28-29 0.1591512968469786 1103 30-31 0.12503653233935097 1103 32-33 0.1388575313639535 1103 34-35 0.11049892769279523 1103 36-37 0.19883605013735206 1103 38-39 0.13035945837146556 1103 40-41 0.2060208448191858 1103 42-43 0.22818351357488353 1103 44-45 0.27741534874196105 1103 46-47 0.13488339541400052 1103 48-49 0.3225572267803045 1103 50-51 0.4204492321410882 1103 52-53 0.571868511565448 1103 54-55 0.41005506623177723 1103 56-57 0.3263740842032732 1103 58-59 0.43453163973101994 1103 60-61 0.4877393676066575 1103 62-63 0.5122958450269977 1103 64-65 0.5767371511273396 1103 66-67 0.6273603490732782 1103 68-69 0.7068368419005395 1103 70-71 0.6087267781651136 1103 72-73 0.4480549189057186 1103 74-75 0.5379803951757935 1103 76-77 0.43433451195137707 1103 78-79 0.6869527780315785 1103 80-81 0.6148903650687245 1103 82-83 0.5905971186312442 1103 84-85 0.5282725648336886 1103 86-87 0.5461456823986133 1103 88-89 0.4513101393035477 1103 90-91 0.5562161369171434 1103 92-93 0.359786306459295 1103 94-95 0.48516071143324524 1103 96-97 0.41106758031379087 1103 98-99 0.440944526989842 1103 100-101 0.6144895056576374 1104 1 0.1972382335391778 1104 2 0.0621497433988587 1104 3 0.18501257102090918 1104 4 0.25560762681327276 1104 5 0.245582516258267 1104 6 0.08665969794671469 1104 7 0.1471735459573722 1104 8 0.14784971513526557 1104 9 0.2529972870806816 1104 10-11 0.29167236148961706 1104 12-13 0.13847715373200487 1104 14-15 0.11915212850488643 1104 16-17 0.3184743019192453 1104 18-19 0.3761422846044695 1104 20-21 0.3769109777094499 1104 22-23 0.10502295374418225 1104 24-25 0.17652526390218526 1104 26-27 0.1341575905532224 1104 28-29 0.1546900215799738 1104 30-31 0.43221593008379955 1104 32-33 0.14001105171873718 1104 34-35 0.25492648595013634 1104 36-37 0.3120670697862309 1104 38-39 0.419737769863481 1104 40-41 0.29935965924275365 1104 42-43 0.3310403242447464 1104 44-45 0.4045002392032089 1104 46-47 0.4171753216019809 1104 48-49 0.4816093303562994 1104 50-51 0.4006832580849107 1104 52-53 0.48776118185296014 1104 54-55 0.6193928508747533 1104 56-57 0.7058100301896246 1104 58-59 0.622801276152039 1104 60-61 0.6832706181257038 1104 62-63 0.6293403747328483 1104 64-65 0.518625137574098 1104 66-67 0.4033141418216317 1104 68-69 0.4249792505905603 1104 70-71 0.6004544842458017 1104 72-73 0.5536016161336192 1104 74-75 0.7247103435206697 1104 76-77 0.7173622329804914 1104 78-79 0.7153938230927857 1104 80-81 0.5779121067266679 1104 82-83 0.6089662451447708 1104 84-85 0.6524724087056661 1104 86-87 0.9052276828304535 1104 88-89 0.6488079385627721 1104 90-91 0.7507367076149052 1104 92-93 0.7582873113258408 1104 94-95 0.849130847796129 1104 96-97 0.9363212883543852 1104 98-99 0.8983240675748227 1104 100-101 0.6131524518266858 1105 1 0.16638408750184297 1105 2 0.21570715712240585 1105 3 0.2515160367487148 1105 4 0.19293479885273968 1105 5 0.08714924527135537 1105 6 0.1456174124000853 1105 7 0.2054179439476158 1105 8 0.2312894041366178 1105 9 0.21411878926280536 1105 10-11 0.2453883918559896 1105 12-13 0.2658536527968067 1105 14-15 0.39137679568499806 1105 16-17 0.4007074042040628 1105 18-19 0.18751225563171658 1105 20-21 0.30059253416963827 1105 22-23 0.27140356033822854 1105 24-25 0.31123480290538197 1105 26-27 0.42640279537956616 1105 28-29 0.4287474903150823 1105 30-31 0.29957482545182756 1105 32-33 0.22004552562492563 1105 34-35 0.06521731369063133 1105 36-37 0.1444129457535226 1105 38-39 0.27573677965553856 1105 40-41 0.27657479108711414 1105 42-43 0.3366395834225031 1105 44-45 0.41834389637121205 1105 46-47 0.4024523964543718 1105 48-49 0.4626468482350532 1105 50-51 0.35748727026080473 1105 52-53 0.26943775059906017 1105 54-55 0.23350148035360974 1105 56-57 0.2819203466894251 1105 58-59 0.34952130402455595 1105 60-61 0.40263468457965956 1105 62-63 0.16379044258042086 1105 64-65 0.2572881734783081 1105 66-67 0.30936908002531993 1105 68-69 0.2115958567445162 1105 70-71 0.26640325015331 1105 72-73 0.30837753574340354 1105 74-75 0.36054028447493636 1105 76-77 0.3407164707037502 1105 78-79 0.19881334119158467 1105 80-81 0.1615608396272421 1105 82-83 0.21600680699609498 1105 84-85 0.3841370582820751 1105 86-87 0.5398822102812204 1105 88-89 0.5401326167451899 1105 90-91 0.3700246747546707 1105 92-93 0.43632578273816947 1105 94-95 0.6890087221497403 1105 96-97 0.6726527512949332 1105 98-99 0.5961841181854517 1105 100-101 0.5405509555124617 1106 1 0.2714366825665948 1106 2 0.3467154810044839 1106 3 0.35194700056021944 1106 4 0.20493755767154198 1106 5 0.3471387845242262 1106 6 0.2814411352607493 1106 7 0.21828825371852645 1106 8 0.39079106877753134 1106 9 0.41545090849906074 1106 10-11 0.48353620953005105 1106 12-13 0.5710447646847996 1106 14-15 0.5558794969154874 1106 16-17 0.577247755994108 1106 18-19 0.5143785911317238 1106 20-21 0.4753596741444568 1106 22-23 0.3358635107174308 1106 24-25 0.2957467656911419 1106 26-27 0.22489858872289403 1106 28-29 0.24566866343638338 1106 30-31 0.4435647400449767 1106 32-33 0.5733010266635432 1106 34-35 0.5381992499315658 1106 36-37 0.39290497891897047 1106 38-39 0.40121645640219583 1106 40-41 0.68451090058349 1106 42-43 0.470026135129352 1106 44-45 0.42705785455803635 1106 46-47 0.6096185502463172 1106 48-49 0.5619433223399639 1106 50-51 0.6935077757642176 1106 52-53 0.7508866928034337 1106 54-55 0.777077877005631 1106 56-57 0.7644578773297965 1106 58-59 0.8395126251274903 1106 60-61 0.9751630959417668 1106 62-63 0.9641131403691645 1106 64-65 0.8054582541154929 1106 66-67 0.8167532563837625 1106 68-69 0.8701166569645764 1106 70-71 0.9298614010672068 1106 72-73 1.0159771262566437 1106 74-75 1.0889793905959593 1106 76-77 0.9938580146395637 1106 78-79 1.029155808708392 1106 80-81 1.2852964374684248 1106 82-83 1.1386963639997134 1106 84-85 1.0587014535302934 1106 86-87 1.3222253295621904 1106 88-89 1.4229454872582146 1106 90-91 1.2247762862110854 1106 92-93 1.2559664791095635 1106 94-95 1.4840360054584814 1106 96-97 1.5926314232949572 1106 98-99 1.574234841968881 1106 100-101 1.7775585246489207 1107 1 0.46379742424519677 1107 2 0.5315473070531773 1107 3 0.43120573890843517 1107 4 0.34158268567315986 1107 5 0.3713680143925515 1107 6 0.4447907581634851 1107 7 0.4147507355281732 1107 8 0.4157778808900616 1107 9 0.5562212300378135 1107 10-11 0.5321541896557065 1107 12-13 0.5646937312272087 1107 14-15 0.5876927393042095 1107 16-17 0.6573342506666435 1107 18-19 0.7598910796981926 1107 20-21 0.7796190360554576 1107 22-23 0.7439972636309591 1107 24-25 0.8540461629386016 1107 26-27 0.9084864535583108 1107 28-29 0.7318696844413282 1107 30-31 0.7925205236735309 1107 32-33 0.7217512218666116 1107 34-35 0.6782721118464679 1107 36-37 0.6949085326683999 1107 38-39 0.7499734444934489 1107 40-41 0.8538627340628153 1107 42-43 0.9170711362592172 1107 44-45 0.8331338502947219 1107 46-47 0.7006302371157958 1107 48-49 0.7259503851656817 1107 50-51 0.6643793899579151 1107 52-53 0.7575822679647075 1107 54-55 0.7679546881763031 1107 56-57 0.8319083841281127 1107 58-59 0.8708945854376253 1107 60-61 1.0399562946029093 1107 62-63 0.9883144495159613 1107 64-65 1.0104460490374194 1107 66-67 1.0514150080107072 1107 68-69 0.9128039035607571 1107 70-71 0.8555533789227141 1107 72-73 0.8889981520490622 1107 74-75 0.8499463708061228 1107 76-77 0.7705443610716856 1107 78-79 0.7776524639920872 1107 80-81 0.8972989227849197 1107 82-83 0.9466293483116175 1107 84-85 0.9609415144049116 1107 86-87 0.9428333535308298 1107 88-89 0.8504026131289244 1107 90-91 0.9399212673497104 1107 92-93 1.00835398000768 1107 94-95 1.2803280964115338 1107 96-97 1.153260339408824 1107 98-99 1.2441486265035557 1107 100-101 1.2319772021969762 1108 1 0.10988329814432873 1108 2 0.19555983532641008 1108 3 0.2696955763026345 1108 4 0.13509846147160687 1108 5 0.22689545373656017 1108 6 0.20073027930168763 1108 7 0.14461309856442028 1108 8 0.23354337524089885 1108 9 0.1809246222781482 1108 10-11 0.07427831576342925 1108 12-13 0.24100104941341272 1108 14-15 0.23170398624562694 1108 16-17 0.2353557098911736 1108 18-19 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-2.618558296809809 2316 76-77 -2.580258143764759 2316 78-79 -2.984553765030501 2316 80-81 -2.710229783698395 2316 82-83 -3.0377904210877134 2316 84-85 -2.9854564227886016 2316 86-87 -3.1941610520274715 2316 88-89 -3.1285578839315384 2316 90-91 -3.693854014552528 2316 92-93 -3.5521333482574704 2316 94-95 -3.629860673264762 2316 96-97 -3.841662001870958 2316 98-99 -4.647043528859928 2316 100-101 -5.026112727459147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2889.0 3 1067.0 4 933.0 5 1148.0 6 1304.0 7 1534.0 8 1948.0 9 2528.0 10 3235.0 11 4423.0 12 6062.0 13 7721.0 14 9088.0 15 11725.0 16 15347.0 17 20599.0 18 17828.0 19 10009.0 20 9661.0 21 10058.0 22 10676.0 23 11904.0 24 13271.0 25 15526.0 26 17701.0 27 21245.0 28 25240.0 29 30792.0 30 37444.0 31 46780.0 32 60077.0 33 77849.0 34 107392.0 35 152353.0 36 228336.0 37 341959.0 38 328460.0 39 60609.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00525519044924 19.993476634940095 15.085078932137886 21.916189242472782 2 14.895489255801936 21.203903989841468 40.91982894555431 22.980777808802284 3 17.6354121652855 27.354050982377565 30.824087279198203 24.18644957313873 4 11.838349003500221 17.820418554821828 39.24935004586037 31.09188239581758 5 12.61307633717877 38.34550030663787 34.60828606241037 14.43313729377299 6 28.961285774249728 38.22966018955917 18.55750433082644 14.251549705364658 7 26.88983648467844 34.14236303657901 22.176522841095373 16.791277637647173 8 25.91785858981634 34.3188664186315 20.82962140343698 18.933653588115178 9 26.95444954237434 16.606772268574943 21.445903507140095 34.99287468191062 10-11 23.684880805392698 27.553862795443806 28.3098964166423 20.451359982521193 12-13 25.238373686720788 25.47180691108385 28.6516971140166 20.638122288178764 14-15 22.528003940544142 26.135079535247584 27.527627270883436 23.809289253324835 16-17 20.695548281249685 29.701659142405113 28.019195051283084 21.58359752506212 18-19 20.57238570438077 27.427413796050796 31.49303510278066 20.507165396787777 20-21 21.52824395765688 26.705213968234837 31.499627555072163 20.26691451903612 22-23 21.30129883672429 26.62513903693748 31.393381841775962 20.680180284562265 24-25 21.035283433368413 27.022126643657536 31.446760351936597 20.495829571037454 26-27 20.727822809500754 27.179437580935478 31.391818074413152 20.700921535150613 28-29 20.48002307418983 26.749582779379992 32.01949259370053 20.75090155272965 30-31 20.744124332117963 26.733698842284713 31.832088280460212 20.69008854513711 32-33 20.60590756546112 27.01190449645303 31.583880960247978 20.798306977837868 34-35 20.95598668839706 27.011016938354494 31.278849818199088 20.754146555049363 36-37 21.288397139855416 26.585809549954654 31.00515108656999 21.12064222361994 38-39 20.670599262920536 27.17525168973165 31.204632938538968 20.949516108808844 40-41 20.73688947526626 26.90383288862736 31.6037099308529 20.755567705253476 42-43 20.910850479625125 26.95685487770906 30.875082022469957 21.257212620195858 44-45 20.758715788668983 27.132138062679317 30.736221936255063 21.372924212396637 46-47 21.13370000388021 27.380265001224874 30.150419092041563 21.33561590285335 48-49 21.185188188078044 27.573911381628857 29.776398163001744 21.464502267291355 50-51 21.195649026405373 27.67502522160846 29.406686998391145 21.72263875359502 52-53 21.333653030593926 28.002815884204722 29.31484880838927 21.348682276812074 54-55 21.22863108632129 27.819016921370505 29.25372788536445 21.698624106943758 56-57 21.196801086073904 28.300936066897336 29.009154481734473 21.493108365294287 58-59 21.21787860596936 27.641100441987255 29.410062992522732 21.73095795952065 60-61 21.415621386693214 27.98865807161156 29.464979392658964 21.130741149036265 62-63 21.19135757958017 27.92103332809175 29.684465182979146 21.203143909348935 64-65 21.104114411007902 27.73076417439872 29.53867724723705 21.62644416735633 66-67 21.201327664307147 28.198993245826433 28.998169537866236 21.601509552000188 68-69 20.898670557858154 27.96709868973571 29.536669525962377 21.597561226443762 70-71 21.177098794745078 27.924631511973597 29.426368888145888 21.471900805135434 72-73 21.232676785256132 28.024587396338706 29.001735724003087 21.741000094402075 74-75 20.93320540440822 28.487566750720177 28.79927042284603 21.77995742202557 76-77 21.046303971435737 28.30026651559675 29.152525584280852 21.50090392868666 78-79 21.06796555177805 28.16333883836017 28.891369465695725 21.87732614416605 80-81 20.956093399506628 28.828124750973576 28.616964831890684 21.598817017629116 82-83 21.365878073371935 28.47790265600276 28.57559943913415 21.580619831491152 84-85 20.9778755462189 28.267039901165838 28.51047636919981 22.244608183415448 86-87 20.98521656666796 28.249340020125256 28.866161237268923 21.899282175937863 88-89 21.27201667036997 28.068442842480014 28.821338444374984 21.838202042775038 90-91 20.96973331247574 28.148395993024295 29.086089722420176 21.795780972079793 92-93 21.15466952953316 27.900173174068037 28.955237197488543 21.98992009891026 94-95 20.881652794912338 27.621269251041102 29.11081765159018 22.386260302456375 96-97 21.174843645241587 27.697447207190507 28.905335683059242 22.222373464508667 98-99 20.797260371589044 27.900086525863472 28.902216504685345 22.400436597862143 100-101 20.602385723330855 27.958341845494495 29.094697249593953 22.344575181580698 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 575.0 1 450.0 2 388.5 3 483.5 4 466.5 5 492.0 6 827.0 7 1834.0 8 3154.0 9 3734.5 10 3355.5 11 2892.5 12 2914.0 13 3142.0 14 3407.5 15 3520.0 16 3557.0 17 3687.5 18 3847.0 19 4082.5 20 4489.5 21 5008.5 22 5692.0 23 6798.5 24 7945.0 25 9499.5 26 12360.5 27 15043.0 28 16430.0 29 18923.0 30 23400.0 31 27803.5 32 33339.5 33 40343.0 34 47326.5 35 53011.0 36 61192.5 37 68735.0 38 68133.5 39 65248.5 40 62251.0 41 59893.5 42 58106.5 43 58239.0 44 59545.5 45 59777.0 46 56332.5 47 51777.0 48 53409.5 49 53302.0 50 49567.5 51 46459.5 52 44743.0 53 44854.5 54 44719.0 55 41864.0 56 37493.0 57 33456.5 58 30439.0 59 27917.5 60 24733.0 61 21100.5 62 17267.5 63 13540.0 64 10423.5 65 7977.5 66 5720.0 67 4603.0 68 3333.0 69 1860.5 70 1308.0 71 942.5 72 720.0 73 565.0 74 407.0 75 318.5 76 217.0 77 100.0 78 56.0 79 36.5 80 22.0 81 20.0 82 16.0 83 10.5 84 7.0 85 8.5 86 7.0 87 5.5 88 4.0 89 2.0 90 3.0 91 1.5 92 1.0 93 1.5 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03654297650941025 2 0.02895639976973871 3 0.023860073410264696 4 0.04882049001177947 5 0.0910389208760585 6 0.10945519112961233 7 0.05577002595651675 8 0.08345234413638696 9 0.06932162104875447 10-11 0.0703930078402348 12-13 0.0687424930533597 14-15 0.06277747470079352 16-17 0.05052891759819405 18-19 0.06048991911898416 20-21 0.056493935950760216 22-23 0.03324194693566004 24-25 0.010018914320329593 26-27 0.002895639976973871 28-29 0.007499707540362326 30-31 0.005820236353717481 32-33 0.006804753945888596 34-35 0.006949535944737291 36-37 0.006254582350263561 38-39 0.0031562475749015197 40-41 0.007239099942434678 42-43 0.004227634366381851 44-45 0.002316511981579097 46-47 0.0014478199884869355 48-49 2.3165119815790969E-4 50-51 0.002316511981579097 52-53 0.005820236353717481 54-55 0.012682903099145553 56-57 0.008571094331842657 58-59 0.005820236353717481 60-61 0.002519206779967268 62-63 0.009121265927467693 64-65 0.0027219015783554387 66-67 0.0228176430185541 68-69 0.04543259123872004 70-71 0.05788384313970768 72-73 0.004459285564539761 74-75 0.014738807482797003 76-77 0.022064776624540896 78-79 0.02507624220059372 80-81 0.07323073501766919 82-83 0.02747962338148204 84-85 0.01190108030536261 86-87 0.03156247574901519 88-89 0.04618545763273324 90-91 0.0430871228573712 92-93 0.041986779666121125 94-95 0.011814211106053393 96-97 0.01688158106575767 98-99 0.03825140409582483 100-101 0.07305699661905077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1726734.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.77612018136196 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56119638480845 33.07776013988868 2 13.063168662898825 11.437096826729206 3 4.482003932684867 5.88614228431649 4 2.0135921601471933 3.525890095954071 5 1.122355727607917 2.4566189609002063 6 0.6892575405521214 1.810381255867178 7 0.45383195586747 1.3906901569537846 8 0.3346430111083827 1.1719498137706725 9 0.25209985078412667 0.9932358029066409 >10 1.6343678602086558 13.99898308885055 >50 0.2251880695978447 6.93601865399496 >100 0.1585984251084815 12.806995784202302 >500 0.0082353144492979 2.3836114752918043 >1k 0.0010626212192642451 0.572745908609379 >5k 2.656553048160613E-4 0.8423144398086766 >10k+ 1.3282765240803064E-4 0.7095653119553555 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12203 0.706709892780243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8493 0.4918534064887817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5993 0.34707140764008815 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1922 0.1113084007148756 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.7912799539477415E-5 0.0 20-21 0.0 0.0 0.0 2.0269479838817096E-4 0.0 22-23 0.0 0.0 0.0 4.343459965460806E-4 0.0 24-25 0.0 5.7912799539477415E-5 0.0 6.370407949342516E-4 0.0 26-27 0.0 5.7912799539477415E-5 0.0 0.0012740815898685033 0.0 28-29 0.0 5.7912799539477415E-5 0.0 0.002490250380197529 0.0 30-31 0.0 5.7912799539477415E-5 0.0 0.004951544360625319 0.0 32-33 0.0 5.7912799539477415E-5 0.0 0.007789271538059713 0.0 34-35 0.0 5.7912799539477415E-5 0.0 0.010395347517336197 0.0 36-37 0.0 5.7912799539477415E-5 0.0 0.013522638692467979 0.0 38-39 0.0 5.7912799539477415E-5 0.0 0.01989304664181049 0.0 40-41 0.0 5.7912799539477415E-5 0.0 0.026350323790462225 0.0 42-43 0.0 5.7912799539477415E-5 0.0 0.03347359813381795 0.0 44-45 0.0 5.7912799539477415E-5 0.0 0.036600889308949724 0.0 46-47 0.0 5.7912799539477415E-5 0.0 0.040944349274410534 0.0 48-49 0.0 5.7912799539477415E-5 0.0 0.04740162642306227 5.7912799539477415E-5 50-51 0.0 5.7912799539477415E-5 0.0 0.05446698796687851 5.7912799539477415E-5 52-53 0.0 5.7912799539477415E-5 0.0 0.07050883343931376 8.686919930921613E-5 54-55 0.0 5.7912799539477415E-5 0.0 0.08044087856033413 1.1582559907895483E-4 56-57 0.0 5.7912799539477415E-5 0.0 0.10823902233928329 1.1582559907895483E-4 58-59 0.0 5.7912799539477415E-5 0.0 0.15752281474737856 1.1582559907895483E-4 60-61 0.0 5.7912799539477415E-5 0.0 0.19811968722455225 1.1582559907895483E-4 62-63 0.0 5.7912799539477415E-5 0.0 0.21957637945392863 1.1582559907895483E-4 64-65 0.0 5.7912799539477415E-5 0.0 0.2646325374956421 1.1582559907895483E-4 66-67 0.0 5.7912799539477415E-5 0.0 0.3579300575537402 1.1582559907895483E-4 68-69 0.0 5.7912799539477415E-5 0.0 0.4847590885451957 1.1582559907895483E-4 70-71 0.0 5.7912799539477415E-5 0.0 0.5924768956886237 1.4478199884869356E-4 72-73 0.0 5.7912799539477415E-5 0.0 0.71921905748077 1.7373839861843226E-4 74-75 0.0 5.7912799539477415E-5 0.0 0.8720219790656812 1.7373839861843226E-4 76-77 0.0 5.7912799539477415E-5 0.0 0.9646535019290754 1.7373839861843226E-4 78-79 0.0 5.7912799539477415E-5 0.0 1.078162589026451 1.7373839861843226E-4 80-81 0.0 5.7912799539477415E-5 0.0 1.2423164193210998 2.0269479838817096E-4 82-83 0.0 5.7912799539477415E-5 0.0 1.3891832789532146 2.3165119815790966E-4 84-85 0.0 5.7912799539477415E-5 0.0 1.5909225161489842 2.3165119815790966E-4 86-87 0.0 5.7912799539477415E-5 0.0 1.9111223848027548 2.3165119815790966E-4 88-89 0.0 5.7912799539477415E-5 0.0 2.3194076215560706 2.3165119815790966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5055 0.0 53.987007 1 GTATCAA 9660 0.0 42.868626 1 ATCAACG 12535 0.0 33.116074 3 TCAACGC 12685 0.0 32.76196 4 TATCAAC 12825 0.0 32.330173 2 CAACGCA 12925 0.0 32.114964 5 AACGCAG 13265 0.0 31.286367 6 ACGCAGA 14945 0.0 27.541143 7 CGCAGAG 14980 0.0 27.38161 8 GTACATG 9855 0.0 26.148163 1 GCAGAGT 15660 0.0 25.311722 9 ACATGGG 9910 0.0 24.470646 3 TACATGG 10305 0.0 24.363228 2 GTATAGG 655 0.0 23.95362 1 GAGTACT 8895 0.0 23.486088 12-13 CATGGGA 6255 0.0 22.80567 4 GTACTTT 9525 0.0 21.931725 14-15 AGAGTAC 14560 0.0 21.12013 10-11 CAGAGTA 15505 0.0 20.967083 10-11 GATATAG 1180 0.0 20.95173 1 >>END_MODULE