FastQCFastQC Report
Thu 26 May 2016
SRR937008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726734
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139200.8061461695895257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104430.6047833655907626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73800.4273964606013434No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24510.14194427167125914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.1120612671088888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60400.052.31911
GTATCAA110700.042.154061
ATCAACG142650.032.4643823
TCAACGC143900.032.149374
TATCAAC146750.031.5915642
CAACGCA147200.031.396365
AACGCAG150800.030.6521746
ACGCAGA171600.026.7152827
CGCAGAG172950.026.534228
GCAGAGT180100.025.0587649
GTACATG97700.024.2706581
GAGTACT110250.023.18079212-13
ACATGGG96650.022.6554833
TACATGG103200.022.2774312
GTACTTT116500.021.83303614-15
AGAGTAC167500.021.54084210-11
AGTACTT117300.021.42309612-13
CAGAGTA176700.021.1586510-11
TAGGACA11150.020.4475214
CATGGGG30500.020.2454
Produced by FastQC (version 0.11.5)