##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937008_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1726734 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16541864583659 33.0 31.0 34.0 31.0 34.0 2 32.2992829237161 33.0 31.0 34.0 31.0 34.0 3 32.41586196831707 34.0 31.0 34.0 31.0 34.0 4 36.088109112347354 37.0 35.0 37.0 35.0 37.0 5 35.93681018616648 37.0 35.0 37.0 35.0 37.0 6 36.03092601408208 37.0 35.0 37.0 35.0 37.0 7 35.98548878981939 37.0 35.0 37.0 35.0 37.0 8 35.937484291153126 37.0 35.0 37.0 35.0 37.0 9 37.6340663935499 39.0 37.0 39.0 35.0 39.0 10-11 37.55460192478981 39.0 37.0 39.0 35.0 39.0 12-13 37.45736459697903 39.0 37.0 39.0 35.0 39.0 14-15 38.63919920497309 40.0 38.0 41.0 34.0 41.0 16-17 38.647665477137764 40.0 38.0 41.0 34.5 41.0 18-19 38.802359251627635 40.0 38.0 41.0 35.0 41.0 20-21 38.82321915245776 40.0 38.0 41.0 35.0 41.0 22-23 38.72593230920339 40.0 38.0 41.0 34.0 41.0 24-25 38.63227891499211 40.0 38.0 41.0 34.0 41.0 26-27 38.43331949217425 40.0 38.0 41.0 34.0 41.0 28-29 38.28013492524037 40.0 38.0 41.0 34.0 41.0 30-31 38.19300743484521 40.0 37.5 41.0 34.0 41.0 32-33 38.01468234250325 40.0 37.0 41.0 33.5 41.0 34-35 37.87426378353585 40.0 37.0 41.0 33.0 41.0 36-37 37.70184290110694 40.0 37.0 41.0 33.0 41.0 38-39 37.52450493243314 40.0 37.0 41.0 33.0 41.0 40-41 37.183011975208686 40.0 36.0 41.0 32.0 41.0 42-43 36.94286815456231 39.5 36.0 41.0 31.0 41.0 44-45 36.644342151136186 39.0 36.0 41.0 31.0 41.0 46-47 36.166798128721624 39.0 35.0 41.0 30.0 41.0 48-49 35.70983341962341 39.0 35.0 40.0 28.5 41.0 50-51 35.40641870722416 39.0 35.0 40.0 27.0 41.0 52-53 35.150919886907886 38.5 35.0 40.0 26.5 41.0 54-55 34.7587897151501 38.0 34.0 40.0 25.5 41.0 56-57 34.573486709591634 38.0 34.0 40.0 25.5 41.0 58-59 34.26681961437025 38.0 34.0 40.0 24.5 41.0 60-61 33.91369284441031 37.5 33.5 40.0 23.5 41.0 62-63 33.5488937497032 37.0 33.0 39.5 22.5 41.0 64-65 33.24019044045001 36.0 33.0 39.0 22.0 40.0 66-67 32.925112669351506 36.0 32.5 39.0 21.5 40.0 68-69 32.48640728682009 35.5 32.0 39.0 20.0 40.0 70-71 32.06557350466256 35.0 31.0 38.0 18.5 40.0 72-73 31.670882139345146 35.0 31.0 37.5 18.0 39.5 74-75 31.186693491875413 35.0 31.0 37.0 14.5 39.0 76-77 29.741776092901397 33.5 29.5 35.5 11.5 37.5 78-79 30.40371707512564 34.0 30.0 36.0 9.5 38.0 80-81 30.26556087967226 34.0 30.0 35.5 7.5 37.0 82-83 29.925583210847762 34.0 30.0 35.0 4.5 37.0 84-85 29.474984855802923 34.0 29.5 35.0 2.0 36.5 86-87 29.10521134117936 34.0 29.0 35.0 2.0 36.0 88-89 28.73840267232822 34.0 29.0 35.0 2.0 36.0 90-91 28.313519627226892 33.5 29.0 35.0 2.0 35.0 92-93 27.84530535681813 33.0 27.5 35.0 2.0 35.0 94-95 27.325762103485538 33.0 26.5 34.5 2.0 35.0 96-97 26.585658532234845 32.5 25.0 34.0 2.0 35.0 98-99 25.784134672740564 32.0 24.5 34.0 2.0 35.0 100-101 22.666243324102034 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16660231817250803 1101 2 0.2884096203849822 1101 3 0.25306994201276467 1101 4 0.13602148867931163 1101 5 0.07509430018423302 1101 6 0.07551267385964167 1101 7 0.09371867902990516 1101 8 0.20047883077346285 1101 9 0.1758852897510721 1101 10-11 0.23065669153028523 1101 12-13 0.24979122866470505 1101 14-15 0.34739586257981614 1101 16-17 0.27300458444396014 1101 18-19 0.14236192077415666 1101 20-21 0.10644459350023538 1101 22-23 0.1353700544245129 1101 24-25 0.07733149451959065 1101 26-27 0.11218822568616815 1101 28-29 0.1434265185764403 1101 30-31 0.1525534834358595 1101 32-33 0.17379373292396139 1101 34-35 0.2116963992011378 1101 36-37 0.058148302420569564 1101 38-39 0.1774107793394748 1101 40-41 0.3336263903953025 1101 42-43 0.339680925501753 1101 44-45 0.18375652121310537 1101 46-47 0.08468799501225277 1101 48-49 0.15520490277427257 1101 50-51 0.13917657761182056 1101 52-53 0.2143320761454035 1101 54-55 0.005403155649737812 1101 56-57 0.05606826427207068 1101 58-59 0.11641236374081387 1101 60-61 0.24440008321657558 1101 62-63 0.18021961064540193 1101 64-65 0.062012264900666025 1101 66-67 0.13120943875533442 1101 68-69 -0.1646704133303487 1101 70-71 -0.239911216635722 1101 72-73 -0.10812360742940896 1101 74-75 -0.0693255088082978 1101 76-77 -2.8394220485949972E-6 1101 78-79 -0.10290721351824672 1101 80-81 -0.1116216476964702 1101 82-83 0.08671867436504499 1101 84-85 0.06070221221081695 1101 86-87 -0.1081526439929803 1101 88-89 0.0330495045200756 1101 90-91 0.18659337663334696 1101 92-93 0.006782316783972675 1101 94-95 0.08188989163132021 1101 96-97 0.034628431371686474 1101 98-99 0.12071055905057548 1101 100-101 0.5664073285797251 1102 1 -0.13481653106435942 1102 2 0.02130211109955127 1102 3 0.017412624235419116 1102 4 0.06611410345178115 1102 5 -0.11855605320428708 1102 6 0.009573376501421649 1102 7 0.03050638620282342 1102 8 0.11890622033797627 1102 9 0.14642178975485365 1102 10-11 0.11432693957045359 1102 12-13 0.1417761299100775 1102 14-15 0.12792192356486254 1102 16-17 0.01978071792219538 1102 18-19 0.06334044009769713 1102 20-21 0.11839096619338108 1102 22-23 -0.009814254772152253 1102 24-25 -0.08533867535400219 1102 26-27 0.06817881039729201 1102 28-29 -0.10942454385847356 1102 30-31 -0.03463665547369743 1102 32-33 0.001546015513746113 1102 34-35 0.1523983601733221 1102 36-37 0.12538239111188432 1102 38-39 0.27915931497712876 1102 40-41 0.2888382091799926 1102 42-43 0.16875989642630884 1102 44-45 0.18013703101040335 1102 46-47 0.059497180340137845 1102 48-49 0.14798004278067367 1102 50-51 0.20786557302016462 1102 52-53 0.20262184212479184 1102 54-55 0.2591382567241496 1102 56-57 0.20259653409296874 1102 58-59 -0.11300751426890798 1102 60-61 -0.14897530036692785 1102 62-63 -0.08102654091200634 1102 64-65 0.06286881916717135 1102 66-67 0.10357541461446829 1102 68-69 0.04864009200388608 1102 70-71 -0.14271210117865962 1102 72-73 -0.11050428364584164 1102 74-75 0.02295793828680459 1102 76-77 0.058989971775435635 1102 78-79 -0.22265082718377727 1102 80-81 0.08560570858421102 1102 82-83 0.3892655175704789 1102 84-85 0.03615261552507931 1102 86-87 -0.06946700218244217 1102 88-89 -0.008584025406371154 1102 90-91 0.14042984209255138 1102 92-93 -0.1443529895407103 1102 94-95 -0.24364964678250445 1102 96-97 -0.020538378600353013 1102 98-99 0.045793201282204876 1102 100-101 0.09063984637522893 1103 1 -0.0010287561174493476 1103 2 0.1440233722262505 1103 3 0.050697706441610535 1103 4 0.08413246756441595 1103 5 0.0031356456554050283 1103 6 -0.015189901010174367 1103 7 -3.758718269963879E-4 1103 8 0.10888309872566992 1103 9 0.09074808211160956 1103 10-11 0.04625750712049381 1103 12-13 0.1292938399177288 1103 14-15 0.11223313616383734 1103 16-17 0.10596865350130003 1103 18-19 0.0833083136572057 1103 20-21 0.10253314618135079 1103 22-23 0.05062181863102211 1103 24-25 0.05758198278727633 1103 26-27 -0.005294031184320147 1103 28-29 -0.062262652619175185 1103 30-31 -0.011019726396703788 1103 32-33 0.02891239523474809 1103 34-35 0.07594952019803713 1103 36-37 -0.04746241748686231 1103 38-39 -0.09663296135943966 1103 40-41 -0.01969496679934224 1103 42-43 -0.18864163183337723 1103 44-45 -0.14798087794351034 1103 46-47 -0.2908636274513512 1103 48-49 -0.2988743115506054 1103 50-51 -0.47477006540576383 1103 52-53 -0.41748427962171064 1103 54-55 -0.21830075579757846 1103 56-57 -0.37664364895397995 1103 58-59 -0.39867390741007824 1103 60-61 -0.36600728236137314 1103 62-63 -0.07855354259106662 1103 64-65 -0.3174668488650738 1103 66-67 -0.17018954605215697 1103 68-69 -0.3205716935644105 1103 70-71 -0.4734180018510017 1103 72-73 -0.27982757951540194 1103 74-75 -0.3882279740231773 1103 76-77 -0.17750816425585825 1103 78-79 -0.09538233271114294 1103 80-81 -0.11407789310120009 1103 82-83 -0.04144622241024365 1103 84-85 -0.3660179680881832 1103 86-87 -0.3677660218957435 1103 88-89 -0.20363638966038167 1103 90-91 -0.4414631285070385 1103 92-93 -0.36785303000609204 1103 94-95 -0.13506821967602534 1103 96-97 -0.14299021198951678 1103 98-99 0.0593708738158405 1103 100-101 0.5635252228006067 1104 1 0.00594385504071937 1104 2 0.06737613851588264 1104 3 0.04860160465943153 1104 4 -0.0016316462429486478 1104 5 -0.14144222734939405 1104 6 0.051289079839229146 1104 7 0.17172039604883338 1104 8 0.07023476879725621 1104 9 0.1468063477617818 1104 10-11 0.16916015963597886 1104 12-13 0.1515130170898189 1104 14-15 0.24438222181832714 1104 16-17 0.0645864221992909 1104 18-19 0.1283205215400045 1104 20-21 0.24395191808495298 1104 22-23 0.1588359797750769 1104 24-25 0.14561161389328703 1104 26-27 0.20708077573181072 1104 28-29 0.08243896015010677 1104 30-31 -0.011449078465403772 1104 32-33 0.001975328116309072 1104 34-35 0.07908121584804206 1104 36-37 0.088366472841507 1104 38-39 0.07308484554009453 1104 40-41 0.21580754951934722 1104 42-43 0.0873976991414196 1104 44-45 0.18501362435688407 1104 46-47 -0.021733792050149248 1104 48-49 0.06882794898388056 1104 50-51 0.030629638947551996 1104 52-53 -0.0054715568486116695 1104 54-55 0.13007235696339592 1104 56-57 0.1951951737742661 1104 58-59 0.0547509435208795 1104 60-61 0.06955918001010986 1104 62-63 0.1190405909814416 1104 64-65 0.13857563788896243 1104 66-67 -0.0990175893029388 1104 68-69 0.03771637925104443 1104 70-71 0.11051326859927713 1104 72-73 0.09494113508589308 1104 74-75 -0.06567411815924018 1104 76-77 0.21098403764757379 1104 78-79 0.31283348936934274 1104 80-81 0.23315166492361428 1104 82-83 0.35056384286455966 1104 84-85 -0.033921201931423894 1104 86-87 -0.06491170263054258 1104 88-89 0.10194352629623538 1104 90-91 -0.025853647881866948 1104 92-93 -0.040252418781481225 1104 94-95 0.053929737372158115 1104 96-97 0.24222828419863518 1104 98-99 0.2874249206874673 1104 100-101 0.9510781651495037 1105 1 0.05288521272583324 1105 2 0.1423642193461916 1105 3 0.1521865241591911 1105 4 0.12930868455985944 1105 5 0.02513642083339107 1105 6 0.07588841236572819 1105 7 0.10982689841432602 1105 8 0.1436165122967239 1105 9 0.13090434167384046 1105 10-11 0.11403033237225912 1105 12-13 0.1272895968382315 1105 14-15 0.13627810328677015 1105 16-17 -0.012567280078179976 1105 18-19 0.018758596869155042 1105 20-21 0.17325198776624973 1105 22-23 0.036348174002526434 1105 24-25 -0.007623065389360306 1105 26-27 0.09827892734365662 1105 28-29 -0.08264337380669673 1105 30-31 0.11549833049047464 1105 32-33 0.2249848267776926 1105 34-35 0.09410670453399916 1105 36-37 0.1589151213910398 1105 38-39 0.07241278774129256 1105 40-41 0.1679695035296973 1105 42-43 0.23125749303147103 1105 44-45 0.1113586568136995 1105 46-47 0.055956272863483036 1105 48-49 0.31965312227027454 1105 50-51 0.3981168946045699 1105 52-53 0.4501047808489673 1105 54-55 0.5606936207621231 1105 56-57 0.2925261966223971 1105 58-59 0.2984805434035209 1105 60-61 0.35451810471763423 1105 62-63 0.3498351411703169 1105 64-65 0.24985208927708413 1105 66-67 0.15988327987959394 1105 68-69 0.10812383148267912 1105 70-71 -0.04449553324911193 1105 72-73 -0.017019293270731595 1105 74-75 -0.06490395642527247 1105 76-77 0.06779996899315321 1105 78-79 0.20217041679076786 1105 80-81 0.2432774580068333 1105 82-83 0.17621389604239468 1105 84-85 -0.0667695167441984 1105 86-87 0.0530119780090601 1105 88-89 0.22123424401362257 1105 90-91 0.10942067639290087 1105 92-93 0.14340915014799194 1105 94-95 0.18008497997310613 1105 96-97 0.42845705890799124 1105 98-99 0.5406816045910894 1105 100-101 0.8693575871614563 1106 1 0.06865729581018343 1106 2 0.14021922417683186 1106 3 0.08425908000968718 1106 4 0.07910895475065871 1106 5 0.00511872428448612 1106 6 0.0880642748692324 1106 7 0.10024657491707956 1106 8 0.15071952220056062 1106 9 0.24238379213230843 1106 10-11 0.20946889784067224 1106 12-13 0.2068437820178488 1106 14-15 0.2870556470979224 1106 16-17 0.11603148116339668 1106 18-19 0.14226570462162869 1106 20-21 0.17484294951812984 1106 22-23 0.2790810374152528 1106 24-25 0.26187498135066534 1106 26-27 0.3947123816801934 1106 28-29 0.2847920629227261 1106 30-31 0.3234312556920713 1106 32-33 0.24093780490633065 1106 34-35 0.24770827744012536 1106 36-37 0.294449886437512 1106 38-39 0.28973430124337796 1106 40-41 0.5467806640086721 1106 42-43 0.41588713922244835 1106 44-45 0.1818853662718709 1106 46-47 0.09194710344952028 1106 48-49 0.13630650975017033 1106 50-51 0.37383478370168177 1106 52-53 0.3106228452855433 1106 54-55 0.41049687199485163 1106 56-57 0.35540094408072065 1106 58-59 0.24471728284826355 1106 60-61 0.21365184780873392 1106 62-63 0.12601698879300471 1106 64-65 0.1940146332982664 1106 66-67 0.20332790092818698 1106 68-69 0.14002877147565584 1106 70-71 0.07374800297122874 1106 72-73 0.12778329400265775 1106 74-75 -0.0391535129952878 1106 76-77 0.1287849729972379 1106 78-79 0.11957489133470389 1106 80-81 0.139078524948836 1106 82-83 0.2279895130164462 1106 84-85 0.2680866886333284 1106 86-87 0.1754723335389592 1106 88-89 0.3966384123232487 1106 90-91 0.204444267274706 1106 92-93 0.37974920302083603 1106 94-95 0.49182794776294614 1106 96-97 0.6068974861725991 1106 98-99 0.659945300784976 1106 100-101 1.2858343817825748 1107 1 0.22014210116739008 1107 2 0.29225648754406564 1107 3 0.25194981669606165 1107 4 0.14900531675509399 1107 5 0.14727279678231753 1107 6 0.17385869571342738 1107 7 0.15980668581073587 1107 8 0.17287666225499265 1107 9 0.2816608018006974 1107 10-11 0.2808082027338443 1107 12-13 0.2825791114469993 1107 14-15 0.48127888704078714 1107 16-17 0.397297570479175 1107 18-19 0.2875599276009737 1107 20-21 0.26398543715017553 1107 22-23 0.21008186126267958 1107 24-25 0.2680737538068385 1107 26-27 0.3828036355327811 1107 28-29 0.3121716808704562 1107 30-31 0.24402900483518408 1107 32-33 0.22211346831198142 1107 34-35 0.3696058664672037 1107 36-37 0.4541817283824017 1107 38-39 0.35449771780155714 1107 40-41 0.3491308069813712 1107 42-43 0.42441040481514847 1107 44-45 0.45466560376180354 1107 46-47 0.6073775832727009 1107 48-49 0.6963414590479999 1107 50-51 0.5310854041931847 1107 52-53 0.5644981483649829 1107 54-55 0.586492534231553 1107 56-57 0.4894799716354967 1107 58-59 0.5121743417092333 1107 60-61 0.3947681703742205 1107 62-63 0.5425868946480534 1107 64-65 0.6595658609351673 1107 66-67 0.4669553536239732 1107 68-69 0.28216087967255277 1107 70-71 0.3505238818503855 1107 72-73 0.47858463732441336 1107 74-75 0.37444413898371565 1107 76-77 0.3445754812176567 1107 78-79 0.47211420650269176 1107 80-81 0.4579050579732815 1107 82-83 0.7570488415714891 1107 84-85 0.5601936495670259 1107 86-87 0.9399621642537639 1107 88-89 0.8772930750651327 1107 90-91 0.8068388600360912 1107 92-93 0.6486145830758367 1107 94-95 1.0051591616032844 1107 96-97 0.8347640787314674 1107 98-99 0.8723322730507626 1107 100-101 1.2010397089073344 1108 1 0.03545387934760669 1108 2 0.09186030248685739 1108 3 0.0934542098826654 1108 4 0.022713621224205838 1108 5 -0.06666473589335453 1108 6 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2316 72-73 -1.2354679876819539 2316 74-75 -0.8083550631856902 2316 76-77 -1.0183399421816084 2316 78-79 -1.2099876961935117 2316 80-81 -1.286115747294243 2316 82-83 -1.1397632196513356 2316 84-85 -1.2196791272894707 2316 86-87 -1.0087305126018222 2316 88-89 -1.6216951604916297 2316 90-91 -1.5624436505489783 2316 92-93 -1.3638253581263662 2316 94-95 -1.8901616763839435 2316 96-97 -1.6969717679031113 2316 98-99 -1.9991412020121437 2316 100-101 -2.6039177101288367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 13.0 5 42.0 6 81.0 7 218.0 8 496.0 9 1054.0 10 1795.0 11 2989.0 12 4782.0 13 6991.0 14 8622.0 15 12032.0 16 16631.0 17 23626.0 18 20812.0 19 11428.0 20 9292.0 21 9156.0 22 9541.0 23 10368.0 24 12055.0 25 13965.0 26 17056.0 27 21009.0 28 26249.0 29 33012.0 30 41083.0 31 51707.0 32 66064.0 33 86539.0 34 118918.0 35 171156.0 36 258960.0 37 388330.0 38 257209.0 39 13447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30272635487969 20.789042448002196 15.562008397963126 21.34622279915499 2 15.240331851680253 22.0485615416914 40.06115800599418 22.64994860063417 3 17.89549153863572 27.578180967388665 30.966954320041744 23.559373173933867 4 12.320426886827965 18.51466409997139 38.57791645962841 30.586992553572234 5 12.560658139730178 38.78229092162149 34.263863993722076 14.393186944926262 6 29.234149556330042 38.836207545574474 17.78334126738687 14.14630163070861 7 26.88257791615124 34.62218716555242 21.741582924467547 16.753651993828793 8 26.373373084678935 34.576605313846834 20.172070509991695 18.877951091482533 9 27.28845323020222 16.601051464788437 21.1288478711834 34.98164743382594 10-11 23.918825945397497 27.97327208475654 27.69430612937488 20.413595840471086 12-13 25.546151226552567 25.742458711430604 28.35888795965315 20.352502102363683 14-15 22.62497527757886 26.42387391000546 27.435415480014587 23.515735332401096 16-17 20.72085219842778 29.926786638822193 27.914577462423278 21.437783700326744 18-19 20.430027195945165 27.42852377444508 31.90514082125268 20.23630820835708 20-21 21.394584514526137 26.70799755605297 32.06438236767504 19.83303556174586 22-23 21.20271024321708 26.684237669531363 31.846400246480517 20.26665184077104 24-25 21.01465579173504 26.9706759652023 32.059938953746794 19.954729289315864 26-27 20.801665808107174 27.1704977531223 31.89981407047253 20.128022368297987 28-29 20.39280571414838 26.72424751618882 32.52578643523906 20.357160334423735 30-31 20.71448179047844 26.813365579180115 32.25079832794165 20.221354302399792 32-33 20.70667657749718 27.12218264517827 31.982553154646887 20.188587622677655 34-35 20.926471593192698 26.84023711816643 31.93387053246186 20.299420756179007 36-37 21.195676409455423 26.524645381137457 31.589123380666038 20.69055482874108 38-39 20.648474393387374 27.212160719296662 31.673535272567 20.465829614748966 40-41 20.817503706449223 26.945943754633063 31.91690025018532 20.319652288732396 42-43 21.03786686310688 27.091839275765693 31.155146073454276 20.715147787673143 44-45 20.799794291949066 27.264482524371363 31.000585503489564 20.935137680190003 46-47 21.31596603177738 27.584282790926096 30.282154463505183 20.817596713791346 48-49 21.35157627240359 27.852845722439845 29.80294568004763 20.992632325108936 50-51 21.590921924041073 27.84817017570124 29.294736607402694 21.26617129285499 52-53 21.56582547886896 28.1991341991342 29.29412616369138 20.940914158305464 54-55 21.44261941908829 28.163399805644644 29.193205207055634 21.200775568211434 56-57 21.407476624147918 28.56410257895205 28.897536584355908 21.130884212544128 58-59 21.357026266098696 27.930080617629322 29.38566302867585 21.32723008759613 60-61 21.475558371467347 28.242414484777427 29.3938334813521 20.88819366240313 62-63 21.270943084152822 28.241561306362563 29.693667262493022 20.79382834699159 64-65 21.214078841372697 27.935963463951868 29.500362823899234 21.3495948707762 66-67 21.473110508045824 28.429538075928313 28.962393744491045 21.134957671534814 68-69 21.282659961128882 28.137371636131142 29.390577113298416 21.18939128944156 70-71 21.37284244615816 28.24438068851768 29.384198053655364 20.998578811668803 72-73 21.41770446764053 28.26681446064464 28.96741698750469 21.348064084210137 74-75 21.381877760174948 28.737035448674753 28.523359430629714 21.357727360520585 76-77 21.362463891327664 28.659021782187395 28.87984354259955 21.0986707838854 78-79 21.340539632716172 28.467160470947977 28.64211544364203 21.55018445269382 80-81 21.29920808386875 29.2190064504621 28.257633934828206 21.224151530840945 82-83 21.63380446456468 28.8940572949861 28.161256200663626 21.310882039785596 84-85 21.260632343172396 28.734444940577024 28.109375805370856 21.895546910879723 86-87 21.156413260463292 28.687511112063458 28.53424426675439 21.62183136071886 88-89 21.39745489778414 28.525519071471468 28.492247301048423 21.584778729695966 90-91 21.193774857640914 28.71829768504509 28.532394745491057 21.555532711822934 92-93 21.277574101620196 28.48992041910347 28.373023399383868 21.859482079892466 94-95 21.10805717614873 28.06413726723398 28.584425858296648 22.243379698320645 96-97 21.47849446019194 28.223992235031602 28.2899840420865 22.00752926268996 98-99 21.04290673310469 28.446125085566752 28.175670432183058 22.335297749145493 100-101 20.67562903152947 28.888047850758262 28.202695079984853 22.233628037727414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1011.0 1 744.5 2 505.0 3 570.5 4 572.0 5 597.0 6 965.0 7 2031.5 8 3398.0 9 4110.5 10 3807.5 11 3273.0 12 3285.0 13 3540.0 14 3805.5 15 4043.5 16 4189.0 17 4396.5 18 4620.5 19 4724.0 20 5005.5 21 5543.5 22 6232.0 23 7301.5 24 8365.0 25 9816.0 26 12573.0 27 15551.0 28 17096.5 29 19571.0 30 23940.5 31 27944.5 32 33492.0 33 39883.0 34 46267.0 35 51811.5 36 60008.5 37 67272.0 38 67383.5 39 65692.0 40 62813.5 41 59607.5 42 57033.5 43 58039.0 44 59622.5 45 59194.5 46 55910.5 47 51810.5 48 53270.5 49 52883.0 50 49408.0 51 46310.0 52 44490.0 53 44238.0 54 43315.0 55 40828.0 56 36951.5 57 32902.5 58 30209.0 59 27967.5 60 24620.0 61 20808.0 62 17226.0 63 13821.0 64 10362.5 65 7880.0 66 5878.0 67 4710.5 68 3390.5 69 1859.0 70 1294.0 71 971.5 72 790.0 73 611.0 74 473.5 75 356.5 76 203.5 77 97.0 78 59.5 79 38.5 80 19.0 81 9.5 82 7.0 83 6.0 84 2.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0210223462328303 2 0.0034168551728291675 3 1.7373839861843226E-4 4 0.0 5 0.0028377271774343935 6 0.0 7 3.474767972368645E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.006138756751184605 14-15 0.003619549971217339 16-17 0.0 18-19 3.474767972368645E-4 20-21 0.001100343191250071 22-23 0.0014767763882566742 24-25 6.080843951645129E-4 26-27 2.606075979276484E-4 28-29 1.4478199884869356E-4 30-31 0.0 32-33 2.0269479838817099E-4 34-35 0.0 36-37 0.0012161687903290259 38-39 0.009179178727007171 40-41 8.107791935526839E-4 42-43 0.0 44-45 8.686919930921613E-4 46-47 1.4478199884869356E-4 48-49 1.1582559907895484E-4 50-51 0.0014188635887171968 52-53 5.212151958552968E-4 54-55 0.0 56-57 3.185203974671258E-4 58-59 1.4478199884869356E-4 60-61 0.0022296427822698804 62-63 2.895639976973871E-5 64-65 5.791279953947742E-5 66-67 0.0 68-69 1.1582559907895484E-4 70-71 5.791279953947742E-4 72-73 3.474767972368645E-4 74-75 0.0031562475749015197 76-77 1.1582559907895484E-4 78-79 2.3165119815790969E-4 80-81 0.0020848607834211873 82-83 2.0269479838817099E-4 84-85 0.002606075979276484 86-87 4.0538959677634197E-4 88-89 0.002606075979276484 90-91 0.0015346891877961513 92-93 2.895639976973871E-5 94-95 0.0 96-97 2.606075979276484E-4 98-99 6.94953594473729E-4 100-101 0.0018532095852632775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1726734.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.6892570291247 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06061454128654 30.875319953466246 2 13.545425979142761 11.293974904269314 3 4.807685746489968 6.012865404020387 4 2.256119172458949 3.7622372827590893 5 1.2190881908708155 2.5411440465192032 6 0.7744012829167655 1.9370528476320568 7 0.5021914329367765 1.4655191407868 8 0.37221925774072967 1.2414035445714633 9 0.27404856852985365 1.0282393090714288 >10 1.7769834762518648 14.376931058098453 >50 0.22821887795061993 6.739558417538083 >100 0.17167079988775308 13.336665984845824 >500 0.009094120739289713 2.5393352360670067 >1k 0.0018188241478579428 0.9982709152261765 >5k 1.3990954983522636E-4 0.4304551185725494 >10k+ 2.798190996704527E-4 1.4210268365559648 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13920 0.8061461695895257 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10443 0.6047833655907626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7380 0.4273964606013434 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2451 0.14194427167125914 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1935 0.1120612671088888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8956399769738707E-5 0.0 12-13 0.0 0.0 0.0 5.7912799539477415E-5 0.0 14-15 0.0 0.0 0.0 5.7912799539477415E-5 0.0 16-17 0.0 0.0 0.0 5.7912799539477415E-5 0.0 18-19 0.0 0.0 0.0 1.1582559907895483E-4 0.0 20-21 0.0 0.0 0.0 2.606075979276484E-4 0.0 22-23 0.0 0.0 0.0 5.501715956250355E-4 0.0 24-25 0.0 0.0 0.0 7.528663940132064E-4 0.0 26-27 0.0 0.0 0.0 0.001389907188947458 0.0 28-29 0.0 0.0 0.0 0.0026929451785857 0.0 30-31 0.0 0.0 0.0 0.00518319555878323 0.0 32-33 0.0 0.0 0.0 0.007963009936678145 0.0 34-35 0.0 0.0 0.0 0.010771780714342798 1.1582559907895483E-4 36-37 0.0 0.0 0.0 0.01392802828924432 1.1582559907895483E-4 38-39 0.0 0.0 0.0 0.019922003041580233 1.1582559907895483E-4 40-41 0.0 0.0 0.0 0.02649510578931092 1.1582559907895483E-4 42-43 0.0 0.0 0.0 0.033618380132666645 1.4478199884869356E-4 44-45 0.0 0.0 0.0 0.036600889308949724 2.0269479838817096E-4 46-47 0.0 0.0 0.0 0.04097330567418027 2.3165119815790966E-4 48-49 0.0 0.0 0.0 0.04734371362352279 2.3165119815790966E-4 50-51 0.0 0.0 0.0 0.05449594436664825 2.3165119815790966E-4 52-53 0.0 0.0 0.0 0.07056674623885323 2.606075979276484E-4 54-55 0.0 0.0 0.0 0.08070148615826178 2.895639976973871E-4 56-57 0.0 0.0 0.0 0.10849962993721093 2.895639976973871E-4 58-59 0.0 0.0 0.0 0.15801507354346414 2.895639976973871E-4 60-61 0.0 0.0 0.0 0.1984671640217891 2.895639976973871E-4 62-63 0.0 0.0 0.0 0.21995281265093525 2.895639976973871E-4 64-65 0.0 0.0 0.0 0.2646325374956421 2.895639976973871E-4 66-67 0.0 0.0 0.0 0.3571192783601875 3.185203974671258E-4 68-69 0.0 0.0 0.0 0.48383248375256405 3.474767972368645E-4 70-71 0.0 0.0 0.0 0.5921294188913868 3.474767972368645E-4 72-73 0.0 0.0 0.0 0.7191901010810003 3.474767972368645E-4 74-75 0.0 0.0 0.0 0.8723404994631484 3.474767972368645E-4 76-77 0.0 0.0 0.0 0.9651747171249306 3.764331970066032E-4 78-79 0.0 0.0 0.0 1.0793787578167802 4.053895967763419E-4 80-81 0.0 0.0 0.0 1.2445171057036 4.053895967763419E-4 82-83 0.0 0.0 0.0 1.392223700929037 4.053895967763419E-4 84-85 0.0 0.0 0.0 1.5941077201236555 4.053895967763419E-4 86-87 0.0 0.0 0.0 1.9129176815884785 4.053895967763419E-4 88-89 0.0 0.0 0.0 2.320507964747321 4.053895967763419E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6040 0.0 52.3191 1 GTATCAA 11070 0.0 42.15406 1 ATCAACG 14265 0.0 32.464382 3 TCAACGC 14390 0.0 32.14937 4 TATCAAC 14675 0.0 31.591564 2 CAACGCA 14720 0.0 31.39636 5 AACGCAG 15080 0.0 30.652174 6 ACGCAGA 17160 0.0 26.715282 7 CGCAGAG 17295 0.0 26.53422 8 GCAGAGT 18010 0.0 25.058764 9 GTACATG 9770 0.0 24.270658 1 GAGTACT 11025 0.0 23.180792 12-13 ACATGGG 9665 0.0 22.655483 3 TACATGG 10320 0.0 22.277431 2 GTACTTT 11650 0.0 21.833036 14-15 AGAGTAC 16750 0.0 21.540842 10-11 AGTACTT 11730 0.0 21.423096 12-13 CAGAGTA 17670 0.0 21.15865 10-11 TAGGACA 1115 0.0 20.447521 4 CATGGGG 3050 0.0 20.245 4 >>END_MODULE