FastQCFastQC Report
Thu 26 May 2016
SRR937007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634185
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57470.3516737701055878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40330.2467896841544868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.15842759540688478No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23640.14465926440396895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18000.1101466480233266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87800.031.1842691
GGTATCA59750.026.1737271
TATCAAC104100.025.9361322
TCAACGC104400.025.6786924
ATCAACG105200.025.6189653
CCGATAG950.001221834625.0173133
CAACGCA107450.024.9467335
AACGCAG108650.024.6712066
GAGTACT52450.023.23609212-13
GTACATG67000.023.1996021
GTATTAG3700.023.1248191
TACATGG67200.022.3524632
ACGCAGA120700.021.9719167
ACATGGG66650.021.8230553
CGCAGAG121900.021.679648
GTACTTT58700.020.72127214-15
GCAGAGT126700.020.596979
AGAGTAC92650.020.16050710-11
AGTACTT57100.020.0956612-13
GTATAGG3506.366463E-1119.013741
Produced by FastQC (version 0.11.5)