FastQCFastQC Report
Thu 26 May 2016
SRR937007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634185
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58400.3573646802534597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44330.27126671704855937No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34980.2140516526586647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.1790494956201409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109350.023.8939591
GTACATG65500.023.6439781
TACATGG66300.022.7812792
ACATGGG65150.022.1627523
GAGTACT57750.021.63025512-13
GGTATCA82350.020.8251381
TATCAAC128100.020.2445452
CAACGCA129100.020.1245235
TCAACGC129150.020.0431794
ATCAACG129050.020.0219063
GTACTTT62700.019.9986614-15
AGTACTT61350.019.93519812-13
AGAGTAC97750.019.75187310-11
ACCGTCC1456.1503315E-419.6538568
AACGCAG133000.019.641546
CATGGGG37150.018.410614
ACGCAGA144100.017.963757
CGCAGAG144800.017.8113068
TACTTTT70300.017.43126514-15
TCCGTCG1650.001464827117.271578
Produced by FastQC (version 0.11.5)