FastQCFastQC Report
Thu 26 May 2016
SRR937006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231290
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47830.38845438523824605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.2750773578929415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.1703091879248593No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18630.15130472918646298No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15100.12263560980760016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74550.027.7897131
GTACATG49900.026.3769421
ACATGGG49000.025.0179183
TACATGG51350.024.9843772
GGTATCA52800.023.6682991
TATCAAC86150.023.6066132
CAACGCA86050.023.525525
ATCAACG86450.023.5237373
TCAACGC86400.023.4273624
AACGCAG87400.023.3243066
CATGGGG29100.021.553044
GAGTACT41800.021.43141712-13
AGAGTAC69450.021.21272510-11
ACGCAGA95950.020.950467
AGTACTT43950.020.92367412-13
CGCAGAG95300.020.893898
GCAGAGT99300.019.6215279
GTACTTT45400.019.52344514-15
CATGGGA23900.018.886584
GTATAGG3408.8766683E-1018.1681061
Produced by FastQC (version 0.11.5)