##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937006_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1231290 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91201991407386 33.0 31.0 34.0 30.0 34.0 2 32.03702783259833 33.0 31.0 34.0 30.0 34.0 3 32.05933776770704 34.0 31.0 34.0 30.0 34.0 4 35.61670849271902 37.0 35.0 37.0 33.0 37.0 5 35.622820781456845 37.0 35.0 37.0 33.0 37.0 6 35.686216082320165 37.0 35.0 37.0 33.0 37.0 7 35.62996044798545 37.0 35.0 37.0 33.0 37.0 8 35.571758074864576 37.0 35.0 37.0 33.0 37.0 9 37.24079055299726 39.0 37.0 39.0 34.0 39.0 10-11 37.07381770338426 39.0 37.0 39.0 33.0 39.0 12-13 37.02094185772645 39.0 37.0 39.0 33.0 39.0 14-15 38.23578076651317 40.0 38.0 41.0 33.0 41.0 16-17 38.17217471107538 40.0 38.0 41.0 33.0 41.0 18-19 38.13144060294488 40.0 38.0 41.0 33.0 41.0 20-21 38.05647857125454 40.0 38.0 41.0 33.0 41.0 22-23 37.94976203818759 40.0 38.0 41.0 33.0 41.0 24-25 37.794816818133825 40.0 37.5 41.0 32.5 41.0 26-27 37.87866343428437 40.0 38.0 41.0 33.0 41.0 28-29 37.8334474413014 40.0 38.0 41.0 33.0 41.0 30-31 37.69486392320249 40.0 37.5 41.0 33.0 41.0 32-33 37.500695611919205 40.0 37.0 41.0 32.0 41.0 34-35 37.33919669614794 40.0 37.0 41.0 32.0 41.0 36-37 37.16926556700696 40.0 36.5 41.0 31.0 41.0 38-39 37.015669744739256 40.0 36.0 41.0 31.0 41.0 40-41 36.84945382485036 39.0 36.0 41.0 30.5 41.0 42-43 36.63468597974482 39.0 36.0 41.0 30.0 41.0 44-45 36.383756872873164 39.0 35.5 41.0 30.0 41.0 46-47 36.03278756426187 39.0 35.0 40.5 29.5 41.0 48-49 35.728959871354434 39.0 35.0 40.0 28.0 41.0 50-51 35.0879362294829 38.5 34.0 40.0 27.0 40.5 52-53 35.31507646452095 38.5 35.0 40.0 28.0 40.5 54-55 35.7066840468127 39.0 35.0 40.5 28.0 41.0 56-57 35.59279373665018 39.0 35.0 41.0 28.0 41.0 58-59 35.35384068740914 39.0 35.0 41.0 27.5 41.0 60-61 35.07896190174532 38.0 35.0 40.0 26.5 41.0 62-63 34.76757831217666 38.0 34.0 40.0 26.0 41.0 64-65 34.450735813658845 37.5 34.0 40.0 26.0 41.0 66-67 34.06772977933712 37.0 34.0 40.0 25.5 41.0 68-69 33.69156941094299 36.0 34.0 39.0 25.0 41.0 70-71 33.26933094559365 36.0 33.0 39.0 23.5 40.5 72-73 32.872396023682484 35.0 33.0 38.5 23.0 40.0 74-75 32.44192635366161 35.0 32.5 37.5 22.0 39.5 76-77 32.0182268190272 35.0 32.0 37.0 20.5 39.0 78-79 31.581964849872897 35.0 32.0 36.5 20.0 39.0 80-81 31.15489324204696 35.0 31.0 36.0 18.0 38.0 82-83 30.764496584882522 35.0 31.0 35.5 17.0 37.0 84-85 30.435248398021585 34.0 31.0 35.0 13.0 37.0 86-87 30.12406744146383 34.0 31.0 35.0 7.5 36.0 88-89 29.779356609734506 34.0 30.0 35.0 6.0 36.0 90-91 29.447809614306948 34.0 30.0 35.0 2.0 36.0 92-93 29.10378139999513 34.0 29.5 35.0 2.0 35.0 94-95 28.715603553996214 34.0 29.0 35.0 2.0 35.0 96-97 28.26749628438467 34.0 29.0 35.0 2.0 35.0 98-99 27.70273737299905 34.0 29.0 35.0 2.0 35.0 100-101 25.565163771329257 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14894170785981586 1101 2 0.15100704648894947 1101 3 0.22149871456828407 1101 4 0.13566857327369064 1101 5 0.07376335798817735 1101 6 -0.07813480228407599 1101 7 -0.07428993938582806 1101 8 0.042450730119782065 1101 9 -0.0794339371699806 1101 10-11 -0.03211877542399577 1101 12-13 -0.01765382228135337 1101 14-15 0.23910515309229652 1101 16-17 0.39032903874042546 1101 18-19 0.18353515365581785 1101 20-21 0.13281932609990577 1101 22-23 0.19384018838722028 1101 24-25 0.1391370524769613 1101 26-27 0.23709261878499177 1101 28-29 0.2887948284028852 1101 30-31 0.399717664724335 1101 32-33 0.3626805945752025 1101 34-35 0.3607554920188818 1101 36-37 0.07908510653804512 1101 38-39 0.21650106903945954 1101 40-41 0.26374642684366734 1101 42-43 0.2899579089484803 1101 44-45 0.3078678182737917 1101 46-47 0.3666378111037645 1101 48-49 0.46679121997620854 1101 50-51 0.47265728723898803 1101 52-53 0.46189063091372873 1101 54-55 0.5087431352822733 1101 56-57 0.532546647376634 1101 58-59 0.4016403412450984 1101 60-61 0.5741769661415006 1101 62-63 0.5149183004968094 1101 64-65 0.5721399504568794 1101 66-67 0.5930486200608911 1101 68-69 0.513998831424054 1101 70-71 0.5583208847161458 1101 72-73 0.6068319731713387 1101 74-75 0.4263594893250726 1101 76-77 0.4903418827107302 1101 78-79 0.5020626582772962 1101 80-81 0.27060554183644925 1101 82-83 0.4186899165945839 1101 84-85 0.44999891447648466 1101 86-87 0.42033777848645926 1101 88-89 0.4509951657539162 1101 90-91 0.5442667776461576 1101 92-93 0.5435124496559247 1101 94-95 0.5433037285259701 1101 96-97 0.5471222571178629 1101 98-99 0.6397745925567335 1101 100-101 0.6967731408331375 1102 1 0.25821193029554834 1102 2 0.14002573990121192 1102 3 0.19284377829168875 1102 4 0.16800721392780815 1102 5 -0.030304325743948368 1102 6 3.0780936521068725E-4 1102 7 0.007217222939097212 1102 8 0.07919238606977075 1102 9 0.1703325794487469 1102 10-11 0.26049942093097656 1102 12-13 0.05239574470127906 1102 14-15 0.18055285411563915 1102 16-17 0.2765710359186855 1102 18-19 0.20593134785502087 1102 20-21 0.19103863485035788 1102 22-23 0.20843149057845523 1102 24-25 0.25278947831942844 1102 26-27 0.32026213075486254 1102 28-29 0.18631079935989447 1102 30-31 0.11218970958783103 1102 32-33 0.18229419583848738 1102 34-35 0.1181066414855394 1102 36-37 0.04379811492263741 1102 38-39 0.18015939158384953 1102 40-41 0.06666303967402598 1102 42-43 0.023104397765990825 1102 44-45 -0.04031985640516211 1102 46-47 -0.1017088230501102 1102 48-49 0.09930333639791655 1102 50-51 0.1321958096122131 1102 52-53 0.20719408126479522 1102 54-55 0.24537496515895185 1102 56-57 0.42341907309769766 1102 58-59 0.2942102366740329 1102 60-61 0.08355055429328928 1102 62-63 0.19022833260782335 1102 64-65 0.15713613927967884 1102 66-67 0.022987563181132487 1102 68-69 -0.036066552594320456 1102 70-71 0.04160958810943072 1102 72-73 0.1045795197531163 1102 74-75 0.057060321020195204 1102 76-77 0.18594155561720527 1102 78-79 0.189036019926359 1102 80-81 0.3896903043251001 1102 82-83 0.135944576940215 1102 84-85 0.1397710416336615 1102 86-87 0.09972498975401223 1102 88-89 0.10080933292715244 1102 90-91 0.2829884680571624 1102 92-93 0.44614727817896593 1102 94-95 0.3121759929548986 1102 96-97 0.2340138887439558 1102 98-99 0.16602402131373495 1102 100-101 0.29024041890685837 1103 1 0.35992293364924777 1103 2 0.35272702790491905 1103 3 0.39274547409121396 1103 4 0.3310279124812041 1103 5 0.32723008202791704 1103 6 0.40110621858664075 1103 7 0.23769605499355606 1103 8 0.4188648997883675 1103 9 0.33682542609992083 1103 10-11 0.5079612408395562 1103 12-13 0.4185244805082178 1103 14-15 0.5282045135977995 1103 16-17 0.5447504140092363 1103 18-19 0.44233381504052716 1103 20-21 0.6404118414365314 1103 22-23 0.7241162954461728 1103 24-25 0.5212095402906485 1103 26-27 0.6337016635183303 1103 28-29 0.6331309865023442 1103 30-31 0.6719387995710591 1103 32-33 0.7414525754645211 1103 34-35 0.7097690602791289 1103 36-37 0.5769257973959014 1103 38-39 0.6429141793576818 1103 40-41 0.6294954606909187 1103 42-43 0.9017546831762004 1103 44-45 0.7792889418207807 1103 46-47 0.7055007851441317 1103 48-49 0.9376155761560341 1103 50-51 0.8273063471664415 1103 52-53 0.8305339612691043 1103 54-55 0.9245787584098082 1103 56-57 0.8424148238361582 1103 58-59 0.8632598256239703 1103 60-61 1.0624232217163296 1103 62-63 1.1137841902729235 1103 64-65 1.0613991467529829 1103 66-67 1.1064853443074512 1103 68-69 1.0771001703924625 1103 70-71 0.9350237273567075 1103 72-73 0.9296166571847877 1103 74-75 0.877451048976333 1103 76-77 0.854473641681281 1103 78-79 0.9212977871422225 1103 80-81 0.9137632483328382 1103 82-83 1.0816149746192387 1103 84-85 0.9053031573764407 1103 86-87 0.9711160983265259 1103 88-89 0.8539024266925921 1103 90-91 0.9682813422001928 1103 92-93 0.9472529400947813 1103 94-95 0.9150341823768855 1103 96-97 1.0222222270020467 1103 98-99 1.053799987707027 1103 100-101 0.9780365876464323 1104 1 0.41158718768327773 1104 2 0.46328484486770094 1104 3 0.47397477373622365 1104 4 0.4409415532264447 1104 5 0.3189479579509893 1104 6 0.4027906568088184 1104 7 0.33477341097722757 1104 8 0.3533987573600115 1104 9 0.3721523802167326 1104 10-11 0.5161559881936455 1104 12-13 0.4965555278198295 1104 14-15 0.5022199315426334 1104 16-17 0.6641094950109263 1104 18-19 0.7417787994184337 1104 20-21 0.6870794352764875 1104 22-23 0.6252763742272762 1104 24-25 0.6524023035819866 1104 26-27 0.4987381569269118 1104 28-29 0.3789540818384509 1104 30-31 0.6733883612339184 1104 32-33 0.7375321850739098 1104 34-35 0.6791900152323862 1104 36-37 0.6301770373525315 1104 38-39 0.7570139744766138 1104 40-41 0.7817775623042635 1104 42-43 0.7678354027507623 1104 44-45 0.8259856061772268 1104 46-47 0.9286589531994665 1104 48-49 0.9022953941481759 1104 50-51 0.9731202588621102 1104 52-53 1.0214634976406174 1104 54-55 1.0924031804185645 1104 56-57 0.959043919062573 1104 58-59 1.0049581119831004 1104 60-61 1.1285069474465743 1104 62-63 1.1217115219100364 1104 64-65 1.15544870934081 1104 66-67 0.896573421365467 1104 68-69 0.8668118172966786 1104 70-71 0.7478636128981648 1104 72-73 0.9343597762265716 1104 74-75 0.8279872348320723 1104 76-77 0.8338629758409297 1104 78-79 0.9044268120047789 1104 80-81 0.8938005576979755 1104 82-83 0.9576931439166749 1104 84-85 1.0432655520205962 1104 86-87 0.927839679338458 1104 88-89 1.0394854656318877 1104 90-91 1.2070143698085936 1104 92-93 1.0978847943774959 1104 94-95 1.2385611165407546 1104 96-97 1.101336976919626 1104 98-99 1.384050304674762 1104 100-101 1.338609435387621 1105 1 0.11199402158496596 1105 2 0.2603096642485241 1105 3 0.1636368640956647 1105 4 0.2368728899745136 1105 5 0.1830857805344408 1105 6 0.17073524180014488 1105 7 0.19125604454382028 1105 8 0.25917605815203615 1105 9 0.30668730154515345 1105 10-11 0.31988902766196503 1105 12-13 0.2241435920799688 1105 14-15 0.36960152293678306 1105 16-17 0.27396214640234007 1105 18-19 0.2838075623077785 1105 20-21 0.22855659332404343 1105 22-23 0.35147468609759613 1105 24-25 0.27256360240266275 1105 26-27 0.23477963631408016 1105 28-29 0.344957025892505 1105 30-31 0.02417945417345635 1105 32-33 0.062449930700182676 1105 34-35 0.17026686460819462 1105 36-37 0.08709552385584374 1105 38-39 -0.052889054932315105 1105 40-41 -0.042670447824832536 1105 42-43 0.030058455374927462 1105 44-45 0.06294000981579018 1105 46-47 0.1378383710841078 1105 48-49 0.3181938939937581 1105 50-51 0.13574135051280933 1105 52-53 -0.020075873002980416 1105 54-55 0.1694803313329487 1105 56-57 0.3017637889146343 1105 58-59 0.10922885471637755 1105 60-61 0.205366658245147 1105 62-63 0.2254168798128191 1105 64-65 0.2736536550598174 1105 66-67 0.2931671702762415 1105 68-69 0.262647464182038 1105 70-71 0.07498088789728996 1105 72-73 0.21246467112173661 1105 74-75 0.3205128068434604 1105 76-77 0.39158005321880296 1105 78-79 0.21665278274419109 1105 80-81 0.3028410814023914 1105 82-83 0.4067467018692277 1105 84-85 0.28005682491057726 1105 86-87 0.43339949948528655 1105 88-89 0.3658302506762219 1105 90-91 0.34738584588045285 1105 92-93 0.3395335132843691 1105 94-95 0.4257219363041749 1105 96-97 0.6044694925862544 1105 98-99 0.679537034140349 1105 100-101 0.8089188285574487 1106 1 0.2783684823337005 1106 2 0.29744819320415417 1106 3 0.16945844270813382 1106 4 0.11434001924109083 1106 5 0.22610621030091949 1106 6 0.15316688799293843 1106 7 0.11585962067724864 1106 8 0.292614501294473 1106 9 0.2520957120163132 1106 10-11 0.24689381646616937 1106 12-13 0.2919994979150644 1106 14-15 0.38692712854246736 1106 16-17 0.5734572398725604 1106 18-19 0.4716175648958014 1106 20-21 0.3761766880104318 1106 22-23 0.20867039465965576 1106 24-25 0.3111727281783345 1106 26-27 0.17004053955144371 1106 28-29 0.22354984521584953 1106 30-31 0.3358056336183779 1106 32-33 0.39577458866359194 1106 34-35 0.3977747113586787 1106 36-37 0.186309000224135 1106 38-39 0.25477993965230894 1106 40-41 0.3572620788774685 1106 42-43 0.1986300549055784 1106 44-45 0.15196657493360277 1106 46-47 0.21116316525893097 1106 48-49 0.27507130566552007 1106 50-51 0.1573598048245799 1106 52-53 0.09679449459271439 1106 54-55 0.09027509575530956 1106 56-57 0.08695862961538836 1106 58-59 0.24719520855082777 1106 60-61 0.3217795014249347 1106 62-63 0.38953363634659155 1106 64-65 0.22429988093336561 1106 66-67 0.2117275810120347 1106 68-69 0.17484655857071374 1106 70-71 0.03018922325757245 1106 72-73 0.13312054854702637 1106 74-75 0.20241726621062384 1106 76-77 0.31949916115078736 1106 78-79 0.27260405408316757 1106 80-81 0.2705173827565268 1106 82-83 0.32772559493743003 1106 84-85 0.3807557354138389 1106 86-87 0.16076310807594396 1106 88-89 0.36535010543594026 1106 90-91 0.3585486146304433 1106 92-93 0.23250636634922373 1106 94-95 0.21636693627498715 1106 96-97 0.21270746171296295 1106 98-99 0.04302181835227614 1106 100-101 0.21246776834616554 1107 1 0.16142226478087807 1107 2 0.1494354606532653 1107 3 0.20741664645009195 1107 4 0.10818834923024667 1107 5 0.1456608881707666 1107 6 0.1285337787090839 1107 7 0.15555263976338551 1107 8 0.2147270010566089 1107 9 0.20484657308096388 1107 10-11 0.4015720034625545 1107 12-13 0.32197263523505626 1107 14-15 0.1733445075652611 1107 16-17 0.26280715455897763 1107 18-19 0.19554379771415142 1107 20-21 0.3343582294060212 1107 22-23 0.3188335476468964 1107 24-25 0.25740931005294954 1107 26-27 0.2898942378479106 1107 28-29 0.30592978774810575 1107 30-31 0.15510019490356086 1107 32-33 0.33725681447079126 1107 34-35 0.3844815962815318 1107 36-37 0.24701379406548796 1107 38-39 0.01918281853661341 1107 40-41 0.21535106507294444 1107 42-43 0.011537725939760435 1107 44-45 0.002677045775804743 1107 46-47 -0.08469077407222159 1107 48-49 -0.12621865270313748 1107 50-51 -0.2434525771688527 1107 52-53 -0.19972784022981926 1107 54-55 -0.024242010306288364 1107 56-57 0.06338506858181603 1107 58-59 -0.033114637292698035 1107 60-61 0.10993407442355618 1107 62-63 -0.019506961081106056 1107 64-65 -0.011299354521561611 1107 66-67 0.011747297225419118 1107 68-69 0.023978939464697646 1107 70-71 -0.12065143802316669 1107 72-73 -0.07820342953996828 1107 74-75 0.1811604923962875 1107 76-77 0.08682696407745283 1107 78-79 0.09190363692403736 1107 80-81 0.14387718409321337 1107 82-83 0.16659497317369087 1107 84-85 0.09767821950871891 1107 86-87 0.07980347292632572 1107 88-89 -0.08372867695838337 1107 90-91 0.22905722950566343 1107 92-93 0.39169285318429203 1107 94-95 0.23674916652230138 1107 96-97 0.38948789392619076 1107 98-99 0.5550606016769066 1107 100-101 0.6291947817785619 1108 1 0.11832729999614244 1108 2 -0.0016695579460588306 1108 3 0.09959439957699345 1108 4 0.17846910013037132 1108 5 -0.07525565650914956 1108 6 0.0522724590991146 1108 7 -5.482775067733314E-4 1108 8 -0.013845748097672583 1108 9 -0.023683466083511462 1108 10-11 0.05677367067333705 1108 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-1.5281476529862417 2316 68-69 -1.6534698225206839 2316 70-71 -1.1350521394777715 2316 72-73 -1.5911120804579575 2316 74-75 -1.8277376411933162 2316 76-77 -1.9818385151609874 2316 78-79 -1.8935281006412268 2316 80-81 -1.9756022148877257 2316 82-83 -1.6812661123183368 2316 84-85 -1.9854449375310033 2316 86-87 -2.5292043471474237 2316 88-89 -2.4674163769720217 2316 90-91 -2.072323225979531 2316 92-93 -2.060066986181706 2316 94-95 -2.729149226969259 2316 96-97 -2.7819340055360975 2316 98-99 -2.666293627848514 2316 100-101 -2.733808820361503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2493.0 3 913.0 4 848.0 5 930.0 6 1001.0 7 1198.0 8 1398.0 9 1829.0 10 2262.0 11 2821.0 12 3666.0 13 4463.0 14 5158.0 15 6212.0 16 8047.0 17 10985.0 18 9577.0 19 6202.0 20 5973.0 21 6347.0 22 6819.0 23 7557.0 24 8633.0 25 9700.0 26 11647.0 27 13569.0 28 16528.0 29 19985.0 30 25024.0 31 31235.0 32 39844.0 33 53157.0 34 74638.0 35 109892.0 36 170777.0 37 261354.0 38 244532.0 39 44053.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0046795598289 18.419998618879912 14.625655524277247 22.949666297013938 2 15.168285107769247 19.893867742504163 40.85441991620881 24.083427233517778 3 19.29354978115851 24.380680211985432 30.00045490435636 26.325315102499697 4 12.750994064052337 16.38560423007363 37.84771929938593 33.015682406488104 5 14.015109960433259 36.60545158010985 34.34285811800786 15.036580341449024 6 31.48802480853996 35.73005109935118 18.046490007671405 14.735434084437452 7 28.253317373210884 32.43618592079911 21.155044788296777 18.155451917693227 8 26.14375420042242 33.972975894146515 20.423378200142867 19.459891705288204 9 26.335801871191244 16.784730117272478 20.851032015687256 36.02843599584902 10-11 24.735238341364663 26.183966026638906 27.848948409723416 21.231847222273014 12-13 25.90731135477553 23.6581763363788 28.24795258905298 22.18655971979269 14-15 22.74848010700502 24.863673836224955 26.778030004148395 25.609816052621635 16-17 22.12886906624002 28.17880361520479 27.157426193902452 22.534901124652738 18-19 21.939184860523135 26.774391034091366 29.6928287436865 21.593595361699 20-21 22.808549260057056 25.788593998714553 29.534379943820728 21.86847679740766 22-23 22.50143385656235 25.717253234504195 29.588016046195364 22.19329686273809 24-25 22.489329755533724 25.813442416419974 29.546779107155697 22.150448720890612 26-27 22.228914268123248 25.974894133232723 29.58307561285355 22.21311598579048 28-29 21.981646830433203 26.19540297573962 29.712883973771902 22.110066220055273 30-31 22.14252296215914 25.79547189795607 29.585649584618036 22.476355555266757 32-33 22.02294993023078 25.985784623481766 29.585397034767652 22.4058684115198 34-35 22.566051283175902 25.957422268898178 29.251001170824658 22.22552527710126 36-37 22.474509147792528 25.79623887084906 29.390461215081555 22.338790766276865 38-39 22.202119883040936 26.015350877192983 29.520711500974663 22.26181773879142 40-41 22.414716645366386 25.944836946258725 29.364049595335302 22.27639681303959 42-43 22.254858604329687 25.993071232457265 29.051726973584024 22.70034318962902 44-45 22.3661966797845 25.998040243633536 28.9942015414097 22.641561535172265 46-47 22.540553886537644 26.354726813036695 28.60111933843541 22.503599961990247 48-49 22.647723244216085 26.327956952132354 28.378830006647547 22.64548979700401 50-51 22.368728832143468 26.49310039553942 28.504990944015336 22.63317982830178 52-53 22.684841320345328 26.582908185538383 28.022150054599493 22.710100439516793 54-55 22.427898809393277 26.423404084238328 28.154612372595984 22.99408473377241 56-57 22.501669263298467 26.709431215792286 28.10194609294913 22.68695342796012 58-59 22.782663926254152 26.347149512082304 28.11846801681122 22.751718544852327 60-61 22.609287564566923 26.205905837686533 28.739723512635578 22.445083085110962 62-63 22.453320142435704 26.335537772752676 28.618359065330683 22.59278301948094 64-65 22.316489609041003 26.547272741302013 28.39986988203694 22.736367767620038 66-67 22.383566150265917 26.609685915438615 28.058938322296612 22.947809611998853 68-69 22.134598942753104 26.478043748007252 28.45675665247121 22.930600656768434 70-71 22.40068458934793 26.455260039168127 28.12108328872564 23.022972082758304 72-73 22.457949447956413 26.357446445578475 28.18750596515739 22.997098141307724 74-75 22.2805128619603 26.771649385931102 28.01225579089775 22.935581961210843 76-77 22.41712295967874 26.595996589835057 27.920177120620167 23.066703329866034 78-79 22.371533409783044 26.676968285556324 27.879406064550043 23.07209224011059 80-81 22.414137664070033 26.749680938264902 27.9075236828701 22.92865771479496 82-83 22.44372810113872 26.671056255244963 28.09366477570448 22.791550867911837 84-85 22.395151552566478 26.501036289842734 27.863220300639806 23.24059185695098 86-87 22.470313766711453 26.432769962357096 28.00473095150287 23.09218531942858 88-89 22.39759626280275 26.316300363176165 27.906363746392405 23.37973962762868 90-91 22.278184373197735 26.362776076485066 28.17352914896555 23.185510401351646 92-93 22.38917883909165 26.315306324299314 28.24108205110083 23.0544327855082 94-95 22.172202257559313 26.366013464775186 28.17769831625326 23.28408596141224 96-97 21.92800870278039 26.57983655476488 28.201858683810084 23.29029605864465 98-99 22.084066512467125 26.503374418450644 27.75937070986096 23.65318835922128 100-101 22.055288559211156 26.46067406599586 27.98444938166464 23.499587993128344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 156.0 1 125.0 2 123.0 3 143.0 4 129.5 5 139.5 6 230.0 7 523.0 8 940.0 9 1153.5 10 1071.5 11 1045.5 12 1137.5 13 1210.5 14 1339.5 15 1426.5 16 1455.5 17 1566.5 18 1701.0 19 1778.5 20 1929.5 21 2212.5 22 2608.0 23 3150.5 24 3893.0 25 4833.5 26 6281.0 27 8041.5 28 9506.5 29 11226.0 30 13559.5 31 16108.5 32 18444.0 33 20684.5 34 23546.0 35 25800.0 36 28201.0 37 31003.0 38 32441.0 39 33335.5 40 34585.0 41 37005.5 42 39321.5 43 41102.5 44 43407.0 45 45595.5 46 46621.0 47 48333.0 48 53256.0 49 53068.0 50 47980.0 51 45233.0 52 43498.0 53 42237.0 54 40886.0 55 38180.5 56 34915.0 57 31711.5 58 28855.5 59 26119.5 60 22530.5 61 18169.5 62 13864.5 63 10693.0 64 8058.0 65 6004.5 66 4545.5 67 3528.0 68 2491.5 69 1633.0 70 1133.5 71 804.5 72 580.5 73 379.0 74 281.0 75 204.0 76 130.0 77 69.5 78 34.5 79 25.0 80 19.5 81 13.0 82 10.0 83 5.5 84 5.5 85 5.5 86 4.5 87 5.0 88 5.5 89 4.0 90 2.0 91 2.0 92 4.0 93 3.0 94 2.0 95 2.0 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032892332431839776 2 0.030293432091546266 3 0.02127849653615314 4 0.03930836764693939 5 0.057744316935896496 6 0.06058686418309253 7 0.05871890456350656 8 0.061642667446336764 9 0.059855923462384976 10-11 0.06468825378261824 12-13 0.0627390785273981 14-15 0.05632304331229848 16-17 0.0486481657448692 18-19 0.0401611318210982 20-21 0.047673578117259134 22-23 0.02793817865815527 24-25 0.014578206596333926 26-27 0.011654443713503724 28-29 0.01360361896872386 30-31 0.010882895174979087 32-33 0.007146975935807161 34-35 0.00864946519503935 36-37 0.005197800680587027 38-39 0.007309407207075506 40-41 0.0069439368467217304 42-43 0.0037765270569890116 44-45 0.0011776267166954983 46-47 0.0021928221621226518 48-49 7.715485385246368E-4 50-51 0.004872938138050338 52-53 0.004182605235159873 54-55 0.005603878858757888 56-57 0.016892852211907835 58-59 0.007187583753624248 60-61 0.016486774033736974 62-63 0.011207757717515776 64-65 0.008080955745600143 66-67 0.01400969714689472 68-69 0.021319104353970227 70-71 0.015187323863590218 72-73 0.014821853503236442 74-75 0.0161213036733832 76-77 0.02148153562523857 78-79 0.03374509660599859 80-81 0.02736966920871606 82-83 0.028181825565057782 84-85 0.017501969479164128 86-87 0.020141477637274727 88-89 0.016811636576273666 90-91 0.01624312712683446 92-93 0.026922983212728114 94-95 0.018354733653322935 96-97 0.03402935133071819 98-99 0.051450105174248154 100-101 0.05912498274167743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1231290.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.05510918502803 #Duplication Level Percentage of deduplicated Percentage of total 1 80.88344410633974 49.38347511173686 2 11.093048762836396 13.545746068196326 3 3.277081313796791 6.0024767226623466 4 1.48056110836311 3.615832805048633 5 0.7983737695667171 2.437239883567916 6 0.49398923492832797 1.809634000486652 7 0.34954962822097424 1.4939253501632277 8 0.25404527417744516 1.2408609562275439 9 0.1941751249363689 1.0669845108605815 >10 1.0757645900485309 12.068747383535255 >50 0.07081389729211726 2.9246275251960094 >100 0.02755576045273372 2.8646613671143015 >500 7.987145203283385E-4 0.3414404917279419 >1k 7.987145203283385E-4 1.2043478234764684 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4783 0.38845438523824605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3387 0.2750773578929415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2097 0.1703091879248593 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1863 0.15130472918646298 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1510 0.12263560980760016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.121563563417229E-5 0.0 0.0 0.0 0.0 3 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 4 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 5 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 6 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 7 2.4364690690251687E-4 0.0 0.0 8.121563563417229E-5 0.0 8 2.4364690690251687E-4 0.0 0.0 8.121563563417229E-5 0.0 9 2.4364690690251687E-4 0.0 0.0 8.121563563417229E-5 0.0 10-11 2.4364690690251687E-4 0.0 0.0 8.121563563417229E-5 0.0 12-13 2.4364690690251687E-4 0.0 0.0 8.121563563417229E-5 0.0 14-15 2.4364690690251687E-4 0.0 0.0 8.121563563417229E-5 0.0 16-17 2.4364690690251687E-4 0.0 0.0 1.6243127126834458E-4 0.0 18-19 2.84254724719603E-4 0.0 0.0 3.2486254253668917E-4 0.0 20-21 3.2486254253668917E-4 0.0 0.0 6.497250850733783E-4 0.0 22-23 3.2486254253668917E-4 0.0 0.0 0.0010558032632442397 0.0 24-25 4.0607817817086146E-4 0.0 0.0 0.0015024892592321874 0.0 26-27 4.0607817817086146E-4 0.0 0.0 0.001989783073037221 0.0 28-29 4.8729381380503375E-4 0.0 0.0 0.004182605235159873 0.0 30-31 4.8729381380503375E-4 0.0 0.0 0.007309407207075506 0.0 32-33 4.8729381380503375E-4 0.0 0.0 0.010476816996808226 0.0 34-35 5.2790163162212E-4 0.0 0.0 0.01413152060034598 0.0 36-37 5.68509449439206E-4 0.0 0.0 0.0187608118314938 0.0 38-39 6.497250850733783E-4 8.121563563417229E-5 0.0 0.023755573422995398 0.0 40-41 6.497250850733783E-4 8.121563563417229E-5 0.0 0.03033403990936335 0.0 42-43 6.497250850733783E-4 8.121563563417229E-5 0.0 0.04077024908835449 0.0 44-45 6.497250850733783E-4 8.121563563417229E-5 0.0 0.0498257924615647 0.0 46-47 7.715485385246367E-4 8.121563563417229E-5 0.0 0.06789627139016803 0.0 48-49 8.933719919758952E-4 8.121563563417229E-5 0.0 0.09083968845682171 0.0 50-51 8.933719919758952E-4 8.121563563417229E-5 0.0 0.10009827091911735 0.0 52-53 8.933719919758952E-4 8.121563563417229E-5 0.0 0.11577288859651261 0.0 54-55 8.933719919758952E-4 8.121563563417229E-5 0.0 0.14590388941679053 0.0 56-57 8.933719919758952E-4 8.121563563417229E-5 0.0 0.19438962389039138 0.0 58-59 8.933719919758952E-4 8.121563563417229E-5 0.0 0.26102705292822975 0.0 60-61 8.933719919758952E-4 8.121563563417229E-5 0.0 0.33330896864264303 0.0 62-63 8.933719919758952E-4 8.121563563417229E-5 0.0 0.40798674560826453 0.0 64-65 8.933719919758952E-4 8.121563563417229E-5 0.0 0.4904206157769494 0.0 66-67 9.745876276100675E-4 8.121563563417229E-5 0.0 0.6022545460452047 0.0 68-69 9.745876276100675E-4 8.121563563417229E-5 0.0 0.7324026021489657 0.0 70-71 0.0010151954454271535 8.121563563417229E-5 0.0 0.792380349064802 0.0 72-73 0.0010558032632442397 8.121563563417229E-5 0.0 0.8818393717158427 0.0 74-75 0.0010558032632442397 8.121563563417229E-5 0.0 0.9846583664287049 8.121563563417229E-5 76-77 0.0010558032632442397 8.121563563417229E-5 0.0 1.0887362034938968 8.121563563417229E-5 78-79 0.0010558032632442397 8.121563563417229E-5 0.0 1.199230075774188 8.121563563417229E-5 80-81 0.0010558032632442397 8.121563563417229E-5 0.0 1.3867163706356749 8.121563563417229E-5 82-83 0.0010558032632442397 8.121563563417229E-5 0.0 1.6071356057468185 8.121563563417229E-5 84-85 0.0010964110810613259 8.121563563417229E-5 0.0 1.9426780043694012 8.121563563417229E-5 86-87 0.001137018898878412 8.121563563417229E-5 0.0 2.3813236524295656 8.121563563417229E-5 88-89 0.001137018898878412 8.121563563417229E-5 0.0 2.850465771670362 8.121563563417229E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7455 0.0 27.789713 1 GTACATG 4990 0.0 26.376942 1 ACATGGG 4900 0.0 25.017918 3 TACATGG 5135 0.0 24.984377 2 GGTATCA 5280 0.0 23.668299 1 TATCAAC 8615 0.0 23.606613 2 CAACGCA 8605 0.0 23.52552 5 ATCAACG 8645 0.0 23.523737 3 TCAACGC 8640 0.0 23.427362 4 AACGCAG 8740 0.0 23.324306 6 CATGGGG 2910 0.0 21.55304 4 GAGTACT 4180 0.0 21.431417 12-13 AGAGTAC 6945 0.0 21.212725 10-11 ACGCAGA 9595 0.0 20.95046 7 AGTACTT 4395 0.0 20.923674 12-13 CGCAGAG 9530 0.0 20.89389 8 GCAGAGT 9930 0.0 19.621527 9 GTACTTT 4540 0.0 19.523445 14-15 CATGGGA 2390 0.0 18.88658 4 GTATAGG 340 8.8766683E-10 18.168106 1 >>END_MODULE