FastQCFastQC Report
Thu 26 May 2016
SRR937006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231290
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45850.37237368938267995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34900.2834425683632613No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30930.2511999610164949No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.1802987111078625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC804.4991268E-429.6815249
ACCGGTA908.9584355E-426.3835778
GTATCAA89450.022.7379781
GTACATG49800.022.7109891
GAGTACT46550.021.7817712-13
TACATGG50600.021.7786922
ACATGGG49850.020.9629253
AGTACTT49500.020.2917812-13
AGAGTAC77900.019.99597210-11
GTACTTT50700.019.81270814-15
ATCAACG105000.019.2262253
TCAACGC105300.019.2142144
CAACGCA105700.019.1864365
TATCAAC105600.019.1169832
CATGGGG27350.019.101914
CGATTCG1250.006038401318.9961749
AACGCAG107350.018.978486
TAATACC3557.8216544E-1118.7301454
GGTATCA67800.018.5038741
CGTAGGG1300.007563351718.269262
Produced by FastQC (version 0.11.5)