##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937006_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1231290 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.291469109632985 33.0 31.0 34.0 31.0 34.0 2 32.3690211079437 34.0 31.0 34.0 31.0 34.0 3 32.505136076797505 34.0 31.0 34.0 31.0 34.0 4 36.149785996800105 37.0 35.0 37.0 35.0 37.0 5 36.067199441236426 37.0 35.0 37.0 35.0 37.0 6 36.1033745096606 37.0 35.0 37.0 35.0 37.0 7 36.06665123569589 37.0 35.0 37.0 35.0 37.0 8 36.04427470376597 37.0 35.0 37.0 35.0 37.0 9 37.75675673480659 39.0 37.0 39.0 35.0 39.0 10-11 37.66352890058394 39.0 37.0 39.0 35.0 39.0 12-13 37.59186990879484 39.0 37.0 39.0 35.0 39.0 14-15 38.85674252207035 40.0 38.0 41.0 35.0 41.0 16-17 38.79270846023276 40.0 38.0 41.0 35.0 41.0 18-19 38.81376645631817 40.0 38.0 41.0 35.0 41.0 20-21 38.88147674390274 40.0 38.0 41.0 35.0 41.0 22-23 38.815661217097514 40.0 38.0 41.0 35.0 41.0 24-25 38.72820497202122 40.0 38.0 41.0 34.5 41.0 26-27 38.56653712772783 40.0 38.0 41.0 34.0 41.0 28-29 38.416435202105106 40.0 38.0 41.0 34.0 41.0 30-31 38.40899381949013 40.0 38.0 41.0 34.0 41.0 32-33 38.34378375524856 40.0 38.0 41.0 34.0 41.0 34-35 38.2327814730892 40.0 38.0 41.0 34.0 41.0 36-37 38.08464334153611 40.0 38.0 41.0 33.5 41.0 38-39 37.95247382826142 40.0 37.5 41.0 33.0 41.0 40-41 37.75510318446507 40.0 37.0 41.0 33.0 41.0 42-43 37.54091603115432 40.0 37.0 41.0 33.0 41.0 44-45 37.29649960610416 40.0 37.0 41.0 32.0 41.0 46-47 36.968528129035406 40.0 36.5 41.0 31.0 41.0 48-49 36.62051344524848 39.5 36.0 41.0 31.0 41.0 50-51 36.39718506606892 39.0 36.0 41.0 30.0 41.0 52-53 36.172490233819815 39.0 35.5 40.5 30.0 41.0 54-55 35.90608223895264 39.0 35.0 40.0 29.0 41.0 56-57 35.64937017274566 38.5 35.0 40.0 28.5 41.0 58-59 35.309083968845684 38.0 34.5 40.0 28.0 41.0 60-61 34.96775901696594 38.0 34.0 40.0 27.5 41.0 62-63 34.639251922780176 37.5 34.0 40.0 27.0 41.0 64-65 34.22358380235363 37.0 34.0 39.5 26.0 41.0 66-67 33.79332001396909 36.0 33.0 39.0 26.0 40.0 68-69 33.358279121896544 36.0 33.0 39.0 25.5 40.0 70-71 32.96683397087607 35.0 32.0 38.0 25.0 40.0 72-73 32.579741571847414 35.0 32.0 37.5 24.0 39.5 74-75 32.09779337117982 35.0 31.5 37.0 22.5 39.0 76-77 30.639194259678874 33.5 29.5 35.5 20.5 38.0 78-79 31.343182759544867 34.0 31.0 36.0 21.5 38.0 80-81 30.99189427348553 34.0 31.0 36.0 19.0 37.0 82-83 30.863638541692048 34.0 31.0 35.0 20.0 37.0 84-85 30.56949418902127 34.0 31.0 35.0 18.5 36.5 86-87 30.23104305240845 34.0 31.0 35.0 18.0 36.0 88-89 29.881487301935366 34.0 30.0 35.0 13.0 36.0 90-91 29.470963379869893 34.0 30.0 35.0 7.0 35.0 92-93 29.074825995500653 34.0 29.0 35.0 2.0 35.0 94-95 28.61064330904986 34.0 29.0 35.0 2.0 35.0 96-97 28.018986185220378 33.5 29.0 35.0 2.0 35.0 98-99 27.30251930901737 33.0 27.0 35.0 2.0 35.0 100-101 24.156758765197473 30.0 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1850211774073216 1101 2 0.21294691359427986 1101 3 0.13379357868781483 1101 4 0.1380075598162378 1101 5 0.12686727702984513 1101 6 0.14083823011610264 1101 7 0.14734142717757237 1101 8 0.17185250991786205 1101 9 0.21090482108261455 1101 10-11 0.19478469896269246 1101 12-13 0.1398689540862108 1101 14-15 0.21300661944095367 1101 16-17 0.23027818648272813 1101 18-19 0.16694755939523986 1101 20-21 0.11341599763739652 1101 22-23 0.2073685482814014 1101 24-25 0.18464814439788313 1101 26-27 0.1416561953297446 1101 28-29 0.18166990651732817 1101 30-31 0.1212150119238018 1101 32-33 0.26109391888510913 1101 34-35 0.18013941656259647 1101 36-37 0.18587790644480862 1101 38-39 0.19352920453512468 1101 40-41 0.2689747103640414 1101 42-43 0.24421756416378315 1101 44-45 0.2728872750949165 1101 46-47 0.44798932392605906 1101 48-49 0.4312760421279691 1101 50-51 0.45590848224657776 1101 52-53 0.3459967107743438 1101 54-55 0.2885828837933886 1101 56-57 0.39434874160616573 1101 58-59 0.36706704923452804 1101 60-61 0.24621693635747732 1101 62-63 0.23000157876723648 1101 64-65 0.39659568175247983 1101 66-67 0.24052020924012396 1101 68-69 0.09740345300650688 1101 70-71 0.21009349877196826 1101 72-73 0.3733174756512554 1101 74-75 0.3086196429688286 1101 76-77 0.1801175113599882 1101 78-79 0.3441985799506284 1101 80-81 0.6439260724077158 1101 82-83 0.31139797215196907 1101 84-85 0.3443450836398654 1101 86-87 0.1967018891106882 1101 88-89 -0.07924306480581222 1101 90-91 0.07488410426605085 1101 92-93 0.1556857771429918 1101 94-95 0.1555169122391291 1101 96-97 0.19190096480119934 1101 98-99 0.4571353024941267 1101 100-101 0.6525593523940216 1102 1 0.08806855827442917 1102 2 0.09193531703622426 1102 3 0.046007295977375406 1102 4 0.04312615723365809 1102 5 0.05957723647266988 1102 6 0.10188227587798337 1102 7 0.04711570489661909 1102 8 0.0842942908259019 1102 9 0.17499000414485977 1102 10-11 0.13792531709314204 1102 12-13 0.15564653395833972 1102 14-15 0.1844154195748402 1102 16-17 0.10936826467473537 1102 18-19 0.1042760239027345 1102 20-21 0.04280342569546747 1102 22-23 0.06712112960602923 1102 24-25 -0.006648020057667736 1102 26-27 0.1219284755410115 1102 28-29 0.161523130669174 1102 30-31 0.21415939128291228 1102 32-33 0.18222357955593083 1102 34-35 0.23178585378396832 1102 36-37 0.27740642697097684 1102 38-39 0.27280797718864847 1102 40-41 0.41242130662292453 1102 42-43 0.43060602501184775 1102 44-45 0.4070307401183726 1102 46-47 0.49887851188950805 1102 48-49 0.6471248160041938 1102 50-51 0.6059355377024573 1102 52-53 0.5007987940444423 1102 54-55 0.45748350442126906 1102 56-57 0.5731063932272846 1102 58-59 0.5297598735552995 1102 60-61 0.5467384476128032 1102 62-63 0.40260931714792036 1102 64-65 0.3382213677860548 1102 66-67 0.31319359178778683 1102 68-69 0.3090751073002238 1102 70-71 0.2065494074840757 1102 72-73 0.26657568095534145 1102 74-75 0.502660808885544 1102 76-77 0.5639225663944281 1102 78-79 0.3438538830367719 1102 80-81 0.4211100850613434 1102 82-83 0.46489215826401775 1102 84-85 0.3620507983244785 1102 86-87 0.4125203123152481 1102 88-89 0.3753607852202485 1102 90-91 0.3627652111463746 1102 92-93 0.43378638008076464 1102 94-95 0.6027845713438573 1102 96-97 0.5254895328304983 1102 98-99 0.6787810546574811 1102 100-101 0.3819352038387578 1103 1 0.19892086298204958 1103 2 0.20865931914184443 1103 3 0.24494339251959474 1103 4 0.1663555231142908 1103 5 0.18909276025755162 1103 6 0.1775033548394731 1103 7 0.2051680841654715 1103 8 0.15658172231105283 1103 9 0.2635025751589666 1103 10-11 0.20579778093068768 1103 12-13 0.32406185775040086 1103 14-15 0.3964760120346753 1103 16-17 0.367707329816362 1103 18-19 0.3994252739426898 1103 20-21 0.3749948231406961 1103 22-23 0.37325032881731346 1103 24-25 0.4227302006675515 1103 26-27 0.4414991340281631 1103 28-29 0.283579536869631 1103 30-31 0.35369512262087 1103 32-33 0.44111235158130313 1103 34-35 0.36157056862628423 1103 36-37 0.5921982933397416 1103 38-39 0.3937768150695078 1103 40-41 0.40665582774661146 1103 42-43 0.5563246271552842 1103 44-45 0.5069223607787023 1103 46-47 0.5833862801602621 1103 48-49 0.48467751523030245 1103 50-51 0.4990620017867968 1103 52-53 0.452194187805361 1103 54-55 0.45492371615019067 1103 56-57 0.47225828519263047 1103 58-59 0.7164868279110124 1103 60-61 0.4729271187826569 1103 62-63 0.5172952513684592 1103 64-65 0.41457777570474974 1103 66-67 0.42367204895334254 1103 68-69 0.6222259235427003 1103 70-71 0.4710548743535341 1103 72-73 0.5921450825233094 1103 74-75 0.465120371650535 1103 76-77 0.5991972688393794 1103 78-79 0.6473164246064833 1103 80-81 0.9318923063717968 1103 82-83 0.8778751075878937 1103 84-85 0.6200735509566364 1103 86-87 0.8922703239003589 1103 88-89 0.7337678510429306 1103 90-91 0.662151747115665 1103 92-93 0.6608591902739924 1103 94-95 0.8348096899415189 1103 96-97 0.8885346772642571 1103 98-99 0.7848254092211526 1103 100-101 1.0826250775168162 1104 1 0.20841073488017514 1104 2 0.13824920683055097 1104 3 0.13062127401892099 1104 4 0.1657341408244406 1104 5 0.057331304987492615 1104 6 0.19794851694394566 1104 7 0.14733592543516494 1104 8 0.16459131029594687 1104 9 0.2028832806607639 1104 10-11 0.2478567068675943 1104 12-13 0.12506211476019757 1104 14-15 0.20779894517050934 1104 16-17 0.27441839068539764 1104 18-19 0.299268590465779 1104 20-21 0.27232172308064406 1104 22-23 0.194063959718207 1104 24-25 0.23630015258888193 1104 26-27 0.25480612991402296 1104 28-29 0.3112989350306563 1104 30-31 0.3711440394468113 1104 32-33 0.35664102865477787 1104 34-35 0.33294618546630517 1104 36-37 0.41964941688216584 1104 38-39 0.33228342299611313 1104 40-41 0.4749893802099763 1104 42-43 0.593336680187825 1104 44-45 0.5854968285404993 1104 46-47 0.4831311533434359 1104 48-49 0.43430392605880996 1104 50-51 0.3826183962763494 1104 52-53 0.3514503129301403 1104 54-55 0.42191430985602807 1104 56-57 0.3878090955002449 1104 58-59 0.3904167190749632 1104 60-61 0.4215406863891147 1104 62-63 0.48427973365890153 1104 64-65 0.36020963329093547 1104 66-67 0.266647158644993 1104 68-69 0.41576480304606633 1104 70-71 0.4268156596803152 1104 72-73 0.5211132575753936 1104 74-75 0.5074647172038382 1104 76-77 0.705378486510817 1104 78-79 0.7726817055565114 1104 80-81 0.7938711100011204 1104 82-83 0.7592224731714374 1104 84-85 0.41635463906606773 1104 86-87 0.5185004527157808 1104 88-89 0.6764308821331859 1104 90-91 0.5394842431300297 1104 92-93 0.6078679332318266 1104 94-95 0.7016850586911119 1104 96-97 0.9865872169372523 1104 98-99 1.1427904994950175 1104 100-101 1.0374252144125222 1105 1 0.07475740859354829 1105 2 0.029279487950425676 1105 3 0.057278094748568265 1105 4 0.07830943427740777 1105 5 0.07765516714545839 1105 6 0.09491959436883946 1105 7 0.15745084243505403 1105 8 0.03411021846690687 1105 9 0.08817703354340978 1105 10-11 0.09635444492298006 1105 12-13 0.18285477942902872 1105 14-15 0.17624986368781492 1105 16-17 0.1444001443591887 1105 18-19 0.14786440961720615 1105 20-21 0.164764858336099 1105 22-23 -0.058316533329787035 1105 24-25 0.08610617898322914 1105 26-27 0.23692882369105916 1105 28-29 0.17590284546720625 1105 30-31 0.16567103322879717 1105 32-33 0.08766618686120609 1105 34-35 0.02590716459125275 1105 36-37 0.11828045606353754 1105 38-39 0.24385115609881325 1105 40-41 0.25455698037631436 1105 42-43 0.14288668249800907 1105 44-45 0.21830450934407963 1105 46-47 0.2292966688406537 1105 48-49 0.14174909183612527 1105 50-51 0.30109152489308855 1105 52-53 0.2241779319993782 1105 54-55 0.1488441770088329 1105 56-57 0.059841716786436905 1105 58-59 0.2196137591333951 1105 60-61 0.10637734894070405 1105 62-63 0.13964951154979843 1105 64-65 0.05924697949207314 1105 66-67 0.03868622410237066 1105 68-69 -0.09104048490301153 1105 70-71 0.0026021525354025243 1105 72-73 0.05243274012871524 1105 74-75 -0.02457314858393289 1105 76-77 -0.1984215885495928 1105 78-79 0.007990819073622646 1105 80-81 0.10737355351734834 1105 82-83 0.09101015110554656 1105 84-85 -0.14137257065643993 1105 86-87 -0.09566472719818009 1105 88-89 -0.0478919327456353 1105 90-91 0.09481839569720307 1105 92-93 0.12916531106209206 1105 94-95 0.1272044224122908 1105 96-97 0.3696283469611714 1105 98-99 0.22686504761270854 1105 100-101 0.49060385251192073 1106 1 0.10921889676163943 1106 2 0.07856077814495421 1106 3 0.05739630679860852 1106 4 0.13110011430317314 1106 5 0.14717358195591856 1106 6 0.16247121363068828 1106 7 0.12108190797803786 1106 8 0.14206068421265883 1106 9 0.18461228350108883 1106 10-11 0.1850862096197048 1106 12-13 0.10415230991606705 1106 14-15 0.1879304790520706 1106 16-17 0.17331685497024552 1106 18-19 0.17998816936361806 1106 20-21 0.07770727787892895 1106 22-23 0.15071131606709542 1106 24-25 -0.08642187570370652 1106 26-27 0.03303298261475618 1106 28-29 0.21120154737242558 1106 30-31 0.14990237275132046 1106 32-33 0.19998052583693493 1106 34-35 0.11999544320975275 1106 36-37 0.1060559679752302 1106 38-39 0.1517391591810764 1106 40-41 0.18347724221356998 1106 42-43 0.2225509018995666 1106 44-45 0.27331057075207355 1106 46-47 0.44353217254074195 1106 48-49 0.4359762088047603 1106 50-51 0.2548978556105155 1106 52-53 0.3100030423792717 1106 54-55 0.458298027608258 1106 56-57 0.5183271528672861 1106 58-59 0.41698555986776853 1106 60-61 0.32231209005144734 1106 62-63 0.4123720080326265 1106 64-65 0.3823112693343944 1106 66-67 0.5213881040144983 1106 68-69 0.6137013263585658 1106 70-71 0.6717944869460979 1106 72-73 0.47741551911401103 1106 74-75 0.4650254071858839 1106 76-77 0.2909935682783953 1106 78-79 0.5144119711497765 1106 80-81 0.821326904999232 1106 82-83 0.787945345605717 1106 84-85 0.6123070834443958 1106 86-87 0.6141315005239569 1106 88-89 0.5227322268785777 1106 90-91 0.5801185083635012 1106 92-93 0.8390464276843623 1106 94-95 0.676589573811281 1106 96-97 0.9016796227756672 1106 98-99 0.746865991363233 1106 100-101 0.47782673525140495 1107 1 0.03302656800725856 1107 2 0.03890000273218419 1107 3 0.004535182942724703 1107 4 0.022686312742365544 1107 5 -0.045909991531111416 1107 6 -0.08556562238750587 1107 7 0.04536689130647886 1107 8 0.026138864020296637 1107 9 -0.011622184034287386 1107 10-11 0.011771443708774143 1107 12-13 0.04620408585530811 1107 14-15 0.020979609952931355 1107 16-17 -0.043629151148643075 1107 18-19 0.04475423887040364 1107 20-21 0.08047389077090372 1107 22-23 -0.11017938105170089 1107 24-25 0.01762713679415384 1107 26-27 0.15527879146580403 1107 28-29 0.09554454816952784 1107 30-31 0.14198707952417777 1107 32-33 0.12787865062946224 1107 34-35 0.032140683514974455 1107 36-37 -0.030164037479771366 1107 38-39 0.0786126223207475 1107 40-41 0.16462077201835257 1107 42-43 0.04568431036242515 1107 44-45 0.25853133948353246 1107 46-47 0.20509205802812858 1107 48-49 0.2988716166713701 1107 50-51 0.15849191516299044 1107 52-53 0.1414798977648104 1107 54-55 0.07326839514384886 1107 56-57 0.14612812377185946 1107 58-59 0.18292873488643124 1107 60-61 0.33025222676462107 1107 62-63 0.2789223155632641 1107 64-65 0.06927594506876034 1107 66-67 0.0783587104704182 1107 68-69 0.2638410841821397 1107 70-71 0.20640225587419536 1107 72-73 0.1670508695974533 1107 74-75 0.22566872661391102 1107 76-77 0.1592403687545456 1107 78-79 0.199785342315689 1107 80-81 0.4840816035896012 1107 82-83 0.2958463670430014 1107 84-85 0.39040559473814085 1107 86-87 0.32952611022750133 1107 88-89 0.316662910381023 1107 90-91 0.1258212724391825 1107 92-93 0.1530308259751294 1107 94-95 0.3175893074045355 1107 96-97 0.49522089784909085 1107 98-99 0.717049843731612 1107 100-101 0.7603347010244832 1108 1 0.102076335091553 1108 2 0.07002095859345303 1108 3 -0.027600866511932054 1108 4 0.045297449401459744 1108 5 0.002734346586912295 1108 6 0.1112449359900296 1108 7 0.12935169825146886 1108 8 0.15994926208909988 1108 9 0.09192789372129795 1108 10-11 0.04645605091143068 1108 12-13 0.11773190995099725 1108 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-0.9354892524905125 2316 54-55 -0.788187864142877 2316 56-57 -0.8185325077056902 2316 58-59 -0.8864597545443189 2316 60-61 -0.6461312178737373 2316 62-63 -0.5165873308409701 2316 64-65 -0.422724351320511 2316 66-67 -0.5651237287459239 2316 68-69 -0.8370598157073275 2316 70-71 -0.6288695830472193 2316 72-73 -0.717754745632007 2316 74-75 -0.6679415585428785 2316 76-77 -0.6108564236736065 2316 78-79 -0.6873240258867952 2316 80-81 -0.5944158038462852 2316 82-83 -0.6335605007679455 2316 84-85 -0.9554079910050852 2316 86-87 -0.7909423756780107 2316 88-89 -0.8311674134972336 2316 90-91 -0.8301118738171311 2316 92-93 -0.7160164789875374 2316 94-95 -0.7936386254879899 2316 96-97 -0.9780835786954611 2316 98-99 -0.9582385884982543 2316 100-101 -1.4783254622927444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 9.0 5 17.0 6 56.0 7 114.0 8 326.0 9 540.0 10 925.0 11 1520.0 12 2245.0 13 3261.0 14 4133.0 15 5886.0 16 7736.0 17 11039.0 18 10066.0 19 6131.0 20 5513.0 21 5517.0 22 5865.0 23 6250.0 24 7338.0 25 8808.0 26 10794.0 27 13087.0 28 16820.0 29 20984.0 30 26429.0 31 33674.0 32 43701.0 33 57782.0 34 80686.0 35 117730.0 36 188982.0 37 292125.0 38 218838.0 39 16358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.915923685888714 19.580085542643374 14.87970819641501 22.624282575052906 2 15.524671704537333 21.00449314819267 39.40910065561628 24.061734491653723 3 19.745317086562626 24.825329887311177 29.57005404580004 25.859298980326162 4 13.313132264750408 17.30448116119056 36.905581797272454 32.47680477678658 5 14.180302171338719 37.47959344456158 33.492091596803824 14.848012787295872 6 31.784632377425304 36.499200025989005 17.176863289720536 14.539304306865159 7 28.44528908705504 32.943660713560575 20.54560664019037 18.065443559194016 8 26.3593083015509 34.73046111064186 19.648774291636077 19.261456296171165 9 26.559099089733678 17.0407735639428 20.163276179090513 36.23685116723301 10-11 24.848573447360085 26.89195883991586 27.13877315660811 21.120694556115943 12-13 26.095731345065477 24.202269246996746 27.638626009561523 22.063373398376253 14-15 22.928838860057336 25.500653785866856 26.109121328038075 25.46138602603773 16-17 22.171746704675584 28.891609612682633 26.531686280242674 22.40495740239911 18-19 22.09924401907658 27.385465803802322 29.081062466700452 21.434227710420647 20-21 22.97077572687085 26.434716646457197 29.004581444702264 21.589926181969687 22-23 22.594311656880183 26.46033834433805 28.87272697739769 22.07262302138408 24-25 22.769778314910678 26.541417779071878 28.8569912083744 21.831812697643045 26-27 22.584462491482075 26.614060607832673 28.995709158424887 21.80576774226036 28-29 22.162402752751472 26.893542055767337 29.074049030847434 21.870006160633753 30-31 22.420065858819065 26.42248997027945 29.009075389135287 22.148368781766198 32-33 22.37679005427268 26.701427170450327 28.887806007151077 22.033976768125918 34-35 22.683931562011168 26.60800522397636 28.794773749518782 21.91328946449369 36-37 22.77130732181607 26.36290789966928 28.853752532778387 22.01203224573626 38-39 22.551520748406233 26.654499704760305 28.844958954778377 21.94902059205509 40-41 22.660410406650133 26.682767574715104 28.677986035501323 21.978835983133436 42-43 22.489413796198264 26.857139144161213 28.29110425872613 22.36234280091439 44-45 22.68312274566148 26.78801887857496 28.20228761806216 22.3265707577014 46-47 22.934674068812996 27.198380201816914 27.753053872665053 22.113891856705038 48-49 23.01924351402452 27.295390457997907 27.413520326946443 22.27184570103113 50-51 22.75746348055897 27.345276634867314 27.591649868184025 22.305610016389686 52-53 23.0685669592422 27.65549454253364 27.036732618666086 22.239205879558074 54-55 22.739935188870028 27.363169735313942 27.26087698067849 22.63601809513754 56-57 22.693377720908114 27.646696454205415 27.278947725346605 22.380978099539867 58-59 23.010893987576296 27.24593318067321 27.209426322541137 22.53374650920935 60-61 22.890017786224202 27.160701378229334 27.791218965475235 22.158061870071226 62-63 22.72958552801758 27.248668976711 27.694218134727862 22.327527360543563 64-65 22.613040020988013 27.49567690806942 27.466923980997077 22.424359089945494 66-67 22.625543490840602 27.611953935610728 27.203028726691365 22.559473846857305 68-69 22.5278846337364 27.337519497010625 27.495972682053655 22.63862318719932 70-71 22.701982269101773 27.450729569080938 27.38863771943824 22.458650442379046 72-73 22.77825589173352 27.308231064555134 27.32528883475529 22.58822420895606 74-75 22.56709027932607 27.89191480963015 26.967488104041966 22.573506807001813 76-77 22.749139079302168 27.580650401221536 26.971630226438386 22.698580293037914 78-79 22.699953336065214 27.652341095899867 26.870182070570976 22.777523497463942 80-81 22.918417883076813 27.658864261540973 26.811458254655495 22.61125960072672 82-83 22.689796720629754 27.80569065278766 27.01805213074596 22.48646049583662 84-85 22.583468084812623 27.713093458103323 26.876199464134448 22.82723899294961 86-87 22.53222230343786 27.527227541846354 27.02758895142493 22.91296120329086 88-89 22.541507854066484 27.60557569279924 26.771966899298533 23.080949553835744 90-91 22.526689813404804 27.57564313414956 26.888875352784712 23.00879169966092 92-93 22.521967912371185 27.55212138218259 26.99091883313726 22.934991872308956 94-95 22.37101462458265 27.627279837372885 26.958749531992236 23.042956006052222 96-97 22.25506502957071 27.716712445362003 27.03486436132629 22.993358163741 98-99 22.260912574332853 27.776487347375024 26.455632566276233 23.50696751201589 100-101 21.874416255033406 27.93894778903802 26.614993900626505 23.57164205530207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 200.0 1 144.5 2 105.5 3 140.0 4 141.5 5 151.0 6 237.0 7 475.5 8 819.0 9 1036.5 10 1052.0 11 1055.5 12 1160.0 13 1262.0 14 1361.5 15 1472.0 16 1606.5 17 1700.0 18 1777.0 19 1937.0 20 2093.5 21 2377.0 22 2833.0 23 3302.5 24 3946.0 25 4922.0 26 6264.0 27 7963.5 28 9582.5 29 11415.0 30 13830.0 31 16300.5 32 18495.0 33 20682.0 34 23737.0 35 25924.0 36 28206.0 37 30990.5 38 32272.0 39 33166.5 40 34394.0 41 36821.0 42 38975.0 43 40809.5 44 43358.0 45 45392.5 46 46470.0 47 48462.0 48 53696.0 49 53294.5 50 48031.5 51 45491.5 52 43422.5 53 41568.0 54 39968.5 55 37749.0 56 34359.5 57 31191.0 58 28940.5 59 26201.5 60 22465.5 61 18246.5 62 14226.5 63 10962.0 64 8022.0 65 6011.0 66 4627.0 67 3640.5 68 2692.5 69 1751.0 70 1200.5 71 839.5 72 654.0 73 453.0 74 312.5 75 237.0 76 157.5 77 74.0 78 28.5 79 18.5 80 15.5 81 8.5 82 5.5 83 3.0 84 2.0 85 2.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02639508158110599 2 0.006659682122002127 3 0.009096151191027297 4 1.6243127126834458E-4 5 0.005522663223123716 6 0.0 7 0.0 8 1.6243127126834458E-4 9 1.6243127126834458E-4 10-11 0.0 12-13 4.0607817817086146E-5 14-15 0.0 16-17 0.0 18-19 0.0034110566966352365 20-21 0.0015430970770492736 22-23 0.0 24-25 0.0049947615915015954 26-27 0.005116585044952855 28-29 0.0069439368467217304 30-31 0.026273258127654735 32-33 6.091172672562922E-4 34-35 0.0035734879679035804 36-37 0.014740637867602272 38-39 0.00706576030017299 40-41 0.007837308838697626 42-43 0.007187583753624248 44-45 0.0043044286886111316 46-47 0.0020709987086713934 48-49 0.0014618814414151012 50-51 0.002274037797756824 52-53 0.0033298410610010636 54-55 0.0016243127126834456 56-57 0.0011776267166954983 58-59 0.0011776267166954983 60-61 0.0 62-63 4.0607817817086146E-5 64-65 0.009014935555393126 66-67 0.0010964110810613259 68-69 9.339798097929814E-4 70-71 0.004020173963891528 72-73 0.014578206596333926 74-75 0.007674877567429282 76-77 0.004060781781708615 78-79 0.01189809062040624 80-81 2.4364690690251685E-4 82-83 0.006334819579465439 84-85 8.933719919758951E-4 86-87 0.0 88-89 0.001380665805780929 90-91 0.0012182345345125845 92-93 4.4668599598794755E-4 94-95 5.68509449439206E-4 96-97 3.2486254253668917E-4 98-99 3.2486254253668917E-4 100-101 0.0012994501701467567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1231290.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.56668147552182 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40269699873458 46.5035246342226 2 11.842719771340978 13.871775933039835 3 3.5800032327324227 6.290067270383346 4 1.5771301397412254 3.6946911415867825 5 0.9136880924486567 2.675583973920881 6 0.5380943423980851 1.8908639973005417 7 0.3820059656383737 1.5660975197904234 8 0.2651878191561582 1.2424936428565603 9 0.20240758338079612 1.0668906417683892 >10 1.1758019186517255 12.546467473992498 >50 0.08405544341086722 3.4257216284592094 >100 0.03398901020554456 3.4674987635327814 >500 0.0015260314854313214 0.5712661928181003 >1k 6.936506751960552E-4 1.1870571863280397 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4585 0.37237368938267995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3490 0.2834425683632613 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3093 0.2511999610164949 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2220 0.1802987111078625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.121563563417229E-5 0.0 3 0.0 0.0 0.0 8.121563563417229E-5 0.0 4 0.0 0.0 0.0 8.121563563417229E-5 0.0 5 0.0 0.0 0.0 8.121563563417229E-5 0.0 6 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 7 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 8 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 9 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 10-11 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 12-13 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 14-15 8.121563563417229E-5 0.0 0.0 8.121563563417229E-5 0.0 16-17 8.121563563417229E-5 0.0 0.0 1.6243127126834458E-4 0.0 18-19 1.2182345345125844E-4 0.0 0.0 3.2486254253668917E-4 0.0 20-21 1.6243127126834458E-4 0.0 0.0 6.091172672562921E-4 0.0 22-23 1.6243127126834458E-4 0.0 0.0 9.745876276100675E-4 0.0 24-25 2.4364690690251687E-4 0.0 0.0 0.001421273623598015 0.0 26-27 2.4364690690251687E-4 0.0 0.0 0.001908567437403049 0.0 28-29 2.4364690690251687E-4 0.0 0.0 0.004060781781708615 0.0 30-31 2.4364690690251687E-4 0.0 0.0 0.007309407207075506 0.0 32-33 2.4364690690251687E-4 0.0 0.0 0.01031438572553988 0.0 34-35 3.2486254253668917E-4 0.0 0.0 0.013928481511260547 0.0 36-37 4.0607817817086146E-4 0.0 0.0 0.018598380560225454 0.0 38-39 5.68509449439206E-4 0.0 0.0 0.023593142151727052 0.0 40-41 5.68509449439206E-4 0.0 0.0 0.03045586336281461 0.0 42-43 5.68509449439206E-4 0.0 0.0 0.04141997417342787 0.0 44-45 6.497250850733783E-4 0.0 0.0 0.05051612536445516 0.0 46-47 7.309407207075506E-4 0.0 0.0 0.06883025119996102 0.0 48-49 7.309407207075506E-4 0.0 0.0 0.0920173151735172 0.0 50-51 7.309407207075506E-4 0.0 0.0 0.10151954454271536 0.0 52-53 7.309407207075506E-4 0.0 0.0 0.1169505153132081 0.0 54-55 7.309407207075506E-4 0.0 0.0 0.14683786922658348 0.0 56-57 8.933719919758952E-4 0.0 0.0 0.1954860349714527 0.0 58-59 8.933719919758952E-4 4.0607817817086146E-5 0.0 0.261636170195486 0.0 60-61 8.933719919758952E-4 8.121563563417229E-5 0.0 0.3334713999139114 0.0 62-63 8.933719919758952E-4 8.121563563417229E-5 0.0 0.40786492215481324 0.0 64-65 8.933719919758952E-4 1.2182345345125844E-4 0.0 0.49025818450568104 0.0 66-67 8.933719919758952E-4 1.6243127126834458E-4 0.0 0.601929683502668 0.0 68-69 8.933719919758952E-4 1.6243127126834458E-4 0.0 0.7318340926995265 0.0 70-71 8.933719919758952E-4 1.6243127126834458E-4 0.0 0.7909184676233869 0.0 72-73 9.745876276100675E-4 2.4364690690251687E-4 0.0 0.8792404713755493 0.0 74-75 0.0010558032632442397 2.4364690690251687E-4 0.0 0.9813285253677038 0.0 76-77 0.0010558032632442397 2.4364690690251687E-4 0.0 1.0845942060765539 0.0 78-79 0.0010558032632442397 2.4364690690251687E-4 0.0 1.1927328249234543 0.0 80-81 0.0010558032632442397 2.4364690690251687E-4 0.0 1.3778232585337329 0.0 82-83 0.0010558032632442397 2.4364690690251687E-4 0.0 1.5986485718230474 0.0 84-85 0.0010558032632442397 2.4364690690251687E-4 0.0 1.9353279893445086 0.0 86-87 0.0010558032632442397 2.4364690690251687E-4 0.0 2.375191871939186 0.0 88-89 0.0010558032632442397 2.4364690690251687E-4 0.0 2.8473795775162634 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTACC 80 4.4991268E-4 29.681524 9 ACCGGTA 90 8.9584355E-4 26.383577 8 GTATCAA 8945 0.0 22.737978 1 GTACATG 4980 0.0 22.710989 1 GAGTACT 4655 0.0 21.78177 12-13 TACATGG 5060 0.0 21.778692 2 ACATGGG 4985 0.0 20.962925 3 AGTACTT 4950 0.0 20.29178 12-13 AGAGTAC 7790 0.0 19.995972 10-11 GTACTTT 5070 0.0 19.812708 14-15 ATCAACG 10500 0.0 19.226225 3 TCAACGC 10530 0.0 19.214214 4 CAACGCA 10570 0.0 19.186436 5 TATCAAC 10560 0.0 19.116983 2 CATGGGG 2735 0.0 19.10191 4 CGATTCG 125 0.0060384013 18.996174 9 AACGCAG 10735 0.0 18.97848 6 TAATACC 355 7.8216544E-11 18.730145 4 GGTATCA 6780 0.0 18.503874 1 CGTAGGG 130 0.0075633517 18.26926 2 >>END_MODULE