FastQCFastQC Report
Thu 26 May 2016
SRR937005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316422
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48740.3702460153355079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.2607066730881131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.16552442909644477No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19360.14706530276765353No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16060.12199735343225804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50900.030.2456511
GTATCAA72800.029.7624861
TATACCG700.00837862327.151745
ATCAACG87850.024.5014693
TATCAAC88350.024.4703732
TCAACGC87850.024.4473824
CAACGCA88600.024.2404355
GAGTACT45200.023.69980612-13
AACGCAG90700.023.678296
GTACATG52350.022.0559251
ACGCAGA99650.021.3592957
CGCAGAG98900.021.233048
GTACTTT50000.021.08928714-15
TACATGG53750.021.0394422
AGAGTAC75000.020.58615910-11
ACATGGG52400.020.130633
GCAGAGT102950.019.934749
AGTACTT49100.019.54369212-13
GAGTACA46950.018.5204371
AGTACAT42900.018.4967272
Produced by FastQC (version 0.11.5)