FastQCFastQC Report
Thu 26 May 2016
SRR937005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316422
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47970.36439682715724897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37510.28493902411232874No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30800.23396752713035787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.18109694307752378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT459.621021E-442.226298
TAGCGTA608.286702E-539.587159
GTACATG55300.025.4361021
CGCAGTA950.001225960725.0024097
GTATCAA91750.024.8092371
TACATGG57100.023.4593242
ACATGGG56500.023.0332453
GAGTACT49350.022.42444812-13
TCAACGC107250.021.1681824
ATCAACG107300.021.158323
GGTATCA68750.021.1510891
AGTACTT52250.021.08893612-13
TATCAAC107600.021.013442
CAACGCA107500.020.9903955
AACGCAG109650.020.578826
GTACTTT53400.020.54583214-15
CATGGGG30550.019.8999394
ACTATCC4350.019.6570668
GCTTATA4850.019.5962161
GTGTAGG7200.019.1403331
Produced by FastQC (version 0.11.5)