FastQCFastQC Report
Thu 26 May 2016
SRR937004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375458
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.36584177779328775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35710.25962261297691386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.16336376683257506No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20410.14838693729652233No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16560.12039626073642379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80100.028.9010261
GTACATG53850.027.0117381
TACATGG54400.026.6551442
ACATGGG54950.025.0040423
TATCAAC95350.024.481662
ATCAACG95200.024.3704173
TCAACGC95400.024.3193244
GGTATCA56400.024.1048151
CAACGCA96450.024.0052815
AACGCAG97850.023.6609616
CATGGGG31350.022.1409054
GAGTACT45200.022.13316712-13
ACGCAGA105600.021.7822867
GGATCGC1100.002863433421.6093446
CGCAGAG106550.021.1866428
AGAGTAC77650.020.8392410-11
GTACTTT49600.020.1712114-15
GCAGAGT110150.019.8894429
GTGTAGC5550.019.699341
AGTACTT48500.019.6962812-13
Produced by FastQC (version 0.11.5)