##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937004_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375458 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836560621989186 33.0 31.0 34.0 30.0 34.0 2 31.933206975422006 33.0 31.0 34.0 30.0 34.0 3 31.95770935935521 33.0 31.0 34.0 30.0 34.0 4 35.54162468065183 37.0 35.0 37.0 33.0 37.0 5 35.542314632653266 37.0 35.0 37.0 33.0 37.0 6 35.606430003678774 37.0 35.0 37.0 33.0 37.0 7 35.55089432029186 37.0 35.0 37.0 33.0 37.0 8 35.51317379374725 37.0 35.0 37.0 33.0 37.0 9 37.14429084712147 39.0 37.0 39.0 33.0 39.0 10-11 37.02210790878384 39.0 37.0 39.0 33.0 39.0 12-13 36.94697911532013 39.0 37.0 39.0 33.0 39.0 14-15 38.13554248839296 40.0 38.0 41.0 33.0 41.0 16-17 38.070341297226086 40.0 38.0 41.0 33.0 41.0 18-19 38.00976983666531 40.0 38.0 41.0 33.0 41.0 20-21 37.94139842874155 40.0 38.0 41.0 33.0 41.0 22-23 37.83269645456277 40.0 38.0 41.0 33.0 41.0 24-25 37.79195148088855 40.0 37.5 41.0 32.5 41.0 26-27 37.81662471700335 40.0 38.0 41.0 33.0 41.0 28-29 37.73169845971306 40.0 37.5 41.0 32.5 41.0 30-31 37.58743305866119 40.0 37.0 41.0 32.0 41.0 32-33 37.397856568503 40.0 37.0 41.0 32.0 41.0 34-35 37.23542558187891 40.0 36.5 41.0 31.0 41.0 36-37 37.06552217515912 40.0 36.0 41.0 31.0 41.0 38-39 36.91387486931626 39.0 36.0 41.0 31.0 41.0 40-41 36.72741770377576 39.0 36.0 41.0 30.0 41.0 42-43 36.51775117815302 39.0 35.5 41.0 30.0 41.0 44-45 36.27097846680887 39.0 35.0 41.0 30.0 41.0 46-47 35.939138454245786 39.0 35.0 40.0 28.5 41.0 48-49 35.73045014824153 39.0 35.0 40.0 28.0 41.0 50-51 35.04708758827969 38.0 34.0 40.0 27.0 40.5 52-53 35.23157268342617 38.5 35.0 40.0 27.5 40.5 54-55 35.6250190845522 39.0 35.0 40.5 28.0 41.0 56-57 35.49714931317423 39.0 35.0 41.0 27.5 41.0 58-59 35.28165709167419 38.5 35.0 40.5 27.0 41.0 60-61 34.99517978738718 38.0 34.5 40.0 26.0 41.0 62-63 34.686129638273215 38.0 34.0 40.0 26.0 41.0 64-65 34.350285868416194 37.0 34.0 40.0 26.0 41.0 66-67 33.97835012046896 37.0 34.0 39.5 25.5 41.0 68-69 33.59170836186928 36.0 33.0 39.0 24.5 41.0 70-71 33.15963410005976 36.0 33.0 39.0 23.5 40.5 72-73 32.771913791624314 35.0 33.0 38.5 22.5 40.0 74-75 32.37549783417596 35.0 32.0 37.5 22.0 39.5 76-77 31.9315057239116 35.0 32.0 37.0 20.5 39.0 78-79 31.472887939871665 35.0 31.5 36.0 19.5 39.0 80-81 31.0399910429835 35.0 31.0 36.0 18.0 37.5 82-83 30.658086251997517 34.5 31.0 35.5 16.0 37.0 84-85 30.30427537591115 34.0 31.0 35.0 11.5 37.0 86-87 29.97177521959958 34.0 30.0 35.0 7.0 36.0 88-89 29.618223893423135 34.0 30.0 35.0 4.5 36.0 90-91 29.26970434575247 34.0 29.5 35.0 2.0 35.5 92-93 28.90020378666597 34.0 29.0 35.0 2.0 35.0 94-95 28.546689539048085 34.0 29.0 35.0 2.0 35.0 96-97 28.083078872637333 34.0 29.0 35.0 2.0 35.0 98-99 27.53399231383292 34.0 28.0 35.0 2.0 35.0 100-101 25.3977893181762 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2209765835266353 1101 2 0.21812984828133608 1101 3 0.3094172934824151 1101 4 0.24436191393679962 1101 5 0.17919020632616167 1101 6 0.2501868652692494 1101 7 0.17829754998732028 1101 8 0.16020666090211932 1101 9 0.27296489292319137 1101 10-11 0.2974504024819993 1101 12-13 0.37580231741175396 1101 14-15 0.34312134469433886 1101 16-17 0.34179754997693124 1101 18-19 0.3762287850695998 1101 20-21 0.36936151938382267 1101 22-23 0.41042173333571697 1101 24-25 0.4700766654229298 1101 26-27 0.3355318201675175 1101 28-29 0.44199930576679947 1101 30-31 0.4021455193718566 1101 32-33 0.27219808241567023 1101 34-35 0.40571875143854896 1101 36-37 0.33808685903034785 1101 38-39 0.3184512291743147 1101 40-41 0.33798950615827295 1101 42-43 0.24819721140778483 1101 44-45 0.3490024274759236 1101 46-47 0.2910422666247925 1101 48-49 0.3820237670359532 1101 50-51 0.5134433359466328 1101 52-53 0.4652821777934406 1101 54-55 0.4241613913808493 1101 56-57 0.4388719474357998 1101 58-59 0.6557542231134263 1101 60-61 0.7020475902045078 1101 62-63 0.5403988366269843 1101 64-65 0.40922383350154945 1101 66-67 0.48149656508764593 1101 68-69 0.4348930843065659 1101 70-71 0.3598268802512621 1101 72-73 0.31841685625980176 1101 74-75 0.3683500971098468 1101 76-77 0.33045934367222785 1101 78-79 0.3888341141656575 1101 80-81 0.4943213869307854 1101 82-83 0.24173080234246314 1101 84-85 0.1568572082574633 1101 86-87 0.09140835602431352 1101 88-89 0.13242708388493796 1101 90-91 0.2663258112423783 1101 92-93 0.27090979122090886 1101 94-95 0.3036058381337021 1101 96-97 0.3050319651496345 1101 98-99 -0.02272134358208433 1101 100-101 0.28495011321317776 1102 1 0.2919925141159787 1102 2 0.18717210566692444 1102 3 0.20316693796359075 1102 4 0.14109373395894664 1102 5 0.3019228327486587 1102 6 0.2715351432197224 1102 7 0.18802036241726228 1102 8 0.17840336904877319 1102 9 0.18280030025574945 1102 10-11 0.1628235801343365 1102 12-13 0.18732338587548014 1102 14-15 0.1574917919621086 1102 16-17 0.35028838511992433 1102 18-19 0.10385828593789626 1102 20-21 0.023623437211291787 1102 22-23 0.07752959189108566 1102 24-25 0.10799182775700444 1102 26-27 0.15050915897946737 1102 28-29 0.06781030694126855 1102 30-31 0.142238046282543 1102 32-33 0.11801667914522795 1102 34-35 0.09853731303448399 1102 36-37 0.04380322527664049 1102 38-39 0.206975583468477 1102 40-41 0.10007651876288293 1102 42-43 0.2737412388588609 1102 44-45 0.11212316334133021 1102 46-47 0.17205095299529205 1102 48-49 0.354656555407729 1102 50-51 0.40502661602185697 1102 52-53 0.48827880831151305 1102 54-55 0.3326232412833079 1102 56-57 0.38197220541698584 1102 58-59 0.1757778797541789 1102 60-61 0.2550876525750496 1102 62-63 0.05767119583316571 1102 64-65 0.28972391882606274 1102 66-67 0.023664234456816757 1102 68-69 0.31539881610650866 1102 70-71 0.2834129191525463 1102 72-73 0.17197154258345648 1102 74-75 0.30865367672431177 1102 76-77 0.19527561154436412 1102 78-79 0.11653095448165729 1102 80-81 0.321719716912483 1102 82-83 0.5529063901614713 1102 84-85 0.4339759749668488 1102 86-87 0.3658002933593103 1102 88-89 0.3898080729132083 1102 90-91 0.29559839463454907 1102 92-93 0.3681571553625247 1102 94-95 0.39563125814782296 1102 96-97 0.13089800900309356 1102 98-99 0.06635828928826726 1102 100-101 0.22456921452851475 1103 1 0.13332813844956704 1103 2 0.1977247554464654 1103 3 0.1780577912531207 1103 4 0.0859674407685489 1103 5 0.08573129648168987 1103 6 0.20174248594759803 1103 7 0.21676593170921166 1103 8 0.23405198148068962 1103 9 -0.004730318458406657 1103 10-11 0.16039839786293442 1103 12-13 0.11847360488933845 1103 14-15 0.22338146372950263 1103 16-17 0.3763214965576367 1103 18-19 0.3666334941451481 1103 20-21 0.24437896298864104 1103 22-23 0.39198558739554556 1103 24-25 0.2556757921960653 1103 26-27 0.33178275340037544 1103 28-29 0.30965402181980295 1103 30-31 0.2725864152056303 1103 32-33 0.2793953137667131 1103 34-35 0.3166434194867449 1103 36-37 0.10109804386976151 1103 38-39 0.15786496705973008 1103 40-41 0.16787445686264135 1103 42-43 0.1001862620570364 1103 44-45 0.17289680819306597 1103 46-47 0.08374748191376824 1103 48-49 0.05915354396713468 1103 50-51 0.1422541094658527 1103 52-53 0.059331335616199965 1103 54-55 0.14355898520999943 1103 56-57 0.26579039509451974 1103 58-59 0.28855020620010663 1103 60-61 0.06291739811552333 1103 62-63 0.19097246957166902 1103 64-65 0.30914158690479354 1103 66-67 0.17448301489542217 1103 68-69 0.31223968524572854 1103 70-71 0.07854940657365717 1103 72-73 0.11414810089589622 1103 74-75 0.18672815584594815 1103 76-77 0.24633952180631624 1103 78-79 0.286906988313536 1103 80-81 0.22255830946025057 1103 82-83 0.2638216015386625 1103 84-85 0.23036782966844171 1103 86-87 0.28894191751999543 1103 88-89 0.10968105928247596 1103 90-91 0.1715178566097073 1103 92-93 0.1102485216055733 1103 94-95 0.3497860375489914 1103 96-97 0.4157231941562536 1103 98-99 0.44616362631025197 1103 100-101 0.5560501156552817 1104 1 0.13804290469619573 1104 2 0.10656478208308329 1104 3 0.12198643585920976 1104 4 0.3031988548996196 1104 5 0.28849612590490636 1104 6 0.11123348755262441 1104 7 0.04919857180755827 1104 8 0.1378080003312192 1104 9 0.22351644587584474 1104 10-11 0.3285857847426996 1104 12-13 0.2668713527293818 1104 14-15 0.1670454496599021 1104 16-17 0.3289006380390447 1104 18-19 0.1420770108613354 1104 20-21 0.2487863836764106 1104 22-23 0.15682781667532453 1104 24-25 0.06308431420541893 1104 26-27 0.12161174974637845 1104 28-29 0.16194566714302994 1104 30-31 0.39737618230599026 1104 32-33 0.18737946839567599 1104 34-35 0.22790165805520246 1104 36-37 0.16929247408524617 1104 38-39 0.33816795855650383 1104 40-41 0.30939107913495434 1104 42-43 0.33593417893714417 1104 44-45 0.40311463935790215 1104 46-47 0.32116232158183067 1104 48-49 0.1717337953075102 1104 50-51 0.2957593428777301 1104 52-53 0.5665678294863667 1104 54-55 0.7161533659087027 1104 56-57 0.6559975972254435 1104 58-59 0.5542824569940876 1104 60-61 0.5593850585328326 1104 62-63 0.45823291078329476 1104 64-65 0.4822689133399294 1104 66-67 0.3219811366725551 1104 68-69 0.38721706119764576 1104 70-71 0.47851162868204256 1104 72-73 0.4873200351239433 1104 74-75 0.5382802509181701 1104 76-77 0.5522604059370444 1104 78-79 0.3870834985754641 1104 80-81 0.4441864725582896 1104 82-83 0.43042530505264764 1104 84-85 0.46356733962302243 1104 86-87 0.5250814865532227 1104 88-89 0.5264395027479694 1104 90-91 0.4135001927774482 1104 92-93 0.5288958596515378 1104 94-95 0.6980576456492535 1104 96-97 0.792162809291316 1104 98-99 0.8819903004898109 1104 100-101 0.7859136456897744 1105 1 0.1856707736611689 1105 2 0.1942348736713484 1105 3 0.2605241756651502 1105 4 0.20724216041499233 1105 5 0.26181306757362677 1105 6 0.22872441896691242 1105 7 0.21397134240983462 1105 8 0.28224858234758443 1105 9 0.3286097245731199 1105 10-11 0.21623636647943556 1105 12-13 0.2759906195656825 1105 14-15 0.37071860817140845 1105 16-17 0.43649826355665766 1105 18-19 0.35212852449535603 1105 20-21 0.28401930079957793 1105 22-23 0.3043569289325987 1105 24-25 0.31192389111490826 1105 26-27 0.4710109479995168 1105 28-29 0.3494672741447147 1105 30-31 0.404095800955119 1105 32-33 0.2957748053505185 1105 34-35 0.15619580433714475 1105 36-37 0.0859855669623073 1105 38-39 0.35313144272365093 1105 40-41 0.4727929172552976 1105 42-43 0.39427498998772137 1105 44-45 0.2530277982761291 1105 46-47 0.2956099767137488 1105 48-49 0.37698887231740486 1105 50-51 0.37346765343897914 1105 52-53 0.5361914037420021 1105 54-55 0.431195779182012 1105 56-57 0.45074490334074113 1105 58-59 0.39831835257888315 1105 60-61 0.5768109465170497 1105 62-63 0.5831792110291119 1105 64-65 0.6439356774499316 1105 66-67 0.552424099272109 1105 68-69 0.46320761512490805 1105 70-71 0.5023662351677132 1105 72-73 0.6441484196065588 1105 74-75 0.8032135488329715 1105 76-77 0.6743541053353503 1105 78-79 0.7063739754801155 1105 80-81 0.5604159363741879 1105 82-83 0.5658985047445562 1105 84-85 0.6566745154353804 1105 86-87 0.7668169672835887 1105 88-89 0.7066107573013269 1105 90-91 0.6417182697034605 1105 92-93 0.5899446952883451 1105 94-95 0.5497473449560495 1105 96-97 0.49435652368742566 1105 98-99 0.6111422447386623 1105 100-101 0.7017664486262944 1106 1 0.2920296888371645 1106 2 0.30247014437991027 1106 3 0.4509306862010014 1106 4 0.29527845386665064 1106 5 0.2665312162892306 1106 6 0.18875665328253177 1106 7 0.27308509770760736 1106 8 0.2822656943916755 1106 9 0.31729587774581347 1106 10-11 0.34232268281348155 1106 12-13 0.3025477160895562 1106 14-15 0.2562095845528063 1106 16-17 0.4301246240636516 1106 18-19 0.3565726815282062 1106 20-21 0.3763182840257997 1106 22-23 0.11423097632898305 1106 24-25 0.25731338909529455 1106 26-27 0.2853319381103461 1106 28-29 0.2522392263105786 1106 30-31 0.35539949994232956 1106 32-33 0.3931108978351432 1106 34-35 0.3805360952418937 1106 36-37 0.30210312272568274 1106 38-39 0.200802015977132 1106 40-41 0.3963016822034646 1106 42-43 0.29679245024202316 1106 44-45 0.3379980511762213 1106 46-47 0.582722020807104 1106 48-49 0.6710547735848849 1106 50-51 0.42850277292481564 1106 52-53 0.5355906914034279 1106 54-55 0.5037243821626802 1106 56-57 0.5379355434505655 1106 58-59 0.5657855710758071 1106 60-61 0.6200652195536449 1106 62-63 0.6483134211888881 1106 64-65 0.3753571709732242 1106 66-67 0.2937626192792706 1106 68-69 0.2987346063898073 1106 70-71 0.39319756214703716 1106 72-73 0.30204204636502396 1106 74-75 0.5053127588932611 1106 76-77 0.4450634592562004 1106 78-79 0.5122802173966683 1106 80-81 0.5042844522028282 1106 82-83 0.3056489872644086 1106 84-85 0.4210798719031388 1106 86-87 0.5943876539541044 1106 88-89 0.7289626684778803 1106 90-91 0.790294587430342 1106 92-93 0.7492775245459597 1106 94-95 0.926944551315362 1106 96-97 1.0011659233730406 1106 98-99 1.0224600711111087 1106 100-101 1.3575861354360903 1107 1 0.1996859388371668 1107 2 0.18994859265576736 1107 3 0.1853434017182387 1107 4 0.16956724697010372 1107 5 0.2414180697375059 1107 6 0.24577712742046032 1107 7 0.2779396235696723 1107 8 0.13973875473650565 1107 9 0.21519458464236862 1107 10-11 0.16143098022727287 1107 12-13 0.31437961264127523 1107 14-15 0.11463630869074137 1107 16-17 0.191646714580898 1107 18-19 0.22936362980406955 1107 20-21 0.38109468488787 1107 22-23 0.3638969246048447 1107 24-25 0.3283290140952957 1107 26-27 0.34832223983448074 1107 28-29 0.2901136875174757 1107 30-31 0.33323624563198706 1107 32-33 0.49783341938686476 1107 34-35 0.38668641851775476 1107 36-37 0.46528469600153954 1107 38-39 0.4844819728736809 1107 40-41 0.64190243651381 1107 42-43 0.7307579674833988 1107 44-45 0.7191268227279437 1107 46-47 0.7353917406346895 1107 48-49 0.8041178123779886 1107 50-51 0.652788872062743 1107 52-53 0.6691817474379036 1107 54-55 0.8923235200937185 1107 56-57 0.8899183020712584 1107 58-59 0.8549019503861501 1107 60-61 0.82105390489847 1107 62-63 0.803984218602686 1107 64-65 0.891642192524948 1107 66-67 0.7023294296241005 1107 68-69 0.49024053311394766 1107 70-71 0.47340769145738193 1107 72-73 0.6075619817098499 1107 74-75 0.5910886209622248 1107 76-77 0.6257908299458528 1107 78-79 0.7973206268794293 1107 80-81 0.6358577280648987 1107 82-83 0.7467885346781991 1107 84-85 0.7922894624203813 1107 86-87 0.8082157789541036 1107 88-89 0.8902800176158117 1107 90-91 0.912775729671722 1107 92-93 0.8608238607528591 1107 94-95 0.8877069435567471 1107 96-97 0.7490323026833892 1107 98-99 0.8825220323180076 1107 100-101 0.8160640449188463 1108 1 0.08200886996138479 1108 2 0.0563369514343961 1108 3 0.14063323950449913 1108 4 0.025138464874331135 1108 5 0.20457137039677065 1108 6 0.20021469247944879 1108 7 0.18608229792776143 1108 8 0.15710787774136037 1108 9 -0.02111375158247597 1108 10-11 0.006051415255029724 1108 12-13 0.1286296993865932 1108 14-15 0.21274742944022051 1108 16-17 0.24303328423738435 1108 18-19 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66-67 -1.3912580703758977 2316 68-69 -1.5119688418860555 2316 70-71 -1.3779610585426205 2316 72-73 -1.993778643290149 2316 74-75 -2.1868113790377706 2316 76-77 -2.311777920054144 2316 78-79 -2.5613887481205744 2316 80-81 -2.413067271935102 2316 82-83 -2.8394958403218027 2316 84-85 -2.861651162579623 2316 86-87 -3.0232123460458986 2316 88-89 -2.753685607384188 2316 90-91 -3.0151398953282786 2316 92-93 -3.0914948892471408 2316 94-95 -3.3432899314432554 2316 96-97 -3.5299926973166116 2316 98-99 -3.941112342681997 2316 100-101 -3.9388828300811483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2609.0 3 981.0 4 939.0 5 1005.0 6 1178.0 7 1307.0 8 1544.0 9 1972.0 10 2483.0 11 3229.0 12 4130.0 13 5161.0 14 5654.0 15 7124.0 16 9024.0 17 11954.0 18 10717.0 19 6941.0 20 6838.0 21 7345.0 22 7988.0 23 8847.0 24 10000.0 25 11624.0 26 13919.0 27 16343.0 28 19637.0 29 23814.0 30 29118.0 31 36535.0 32 47202.0 33 62602.0 34 86129.0 35 124135.0 36 191301.0 37 286511.0 38 261902.0 39 45705.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94584232106832 18.396723834236834 14.652274654514619 23.00515919018023 2 15.151684833552828 19.878105272190595 40.773211957347755 24.196997936908822 3 19.30770539702116 24.365203909927967 29.922060318536086 26.405030374514787 4 12.790523447717053 16.360596023118227 37.765247889581815 33.0836326395829 5 14.116714824271433 36.61205247444712 34.268689601377275 15.00254309990417 6 31.647668303277054 35.62429675002075 17.96571706388312 14.762317882819085 7 28.364403993261156 32.24724233950867 21.194777684990935 18.193575982239242 8 26.204177796081236 33.88250976271882 20.41210424335382 19.501208197846125 9 26.279080595572925 16.790174472660713 20.751712206684118 36.179032725082244 10-11 24.67392253295696 26.19521723947269 27.835135174460902 21.29572505310945 12-13 25.875893517422206 23.58576803303764 28.20472247897226 22.333615970567898 14-15 22.820174364954454 24.815743404390442 26.724708297521072 25.639373933134035 16-17 22.10804712577712 28.140572844269197 27.142456042345408 22.608923987608282 18-19 22.030562571288904 26.72173613212598 29.515642822225846 21.732058474359274 20-21 22.84387896836582 25.830789503259368 29.390466429262734 21.93486509911208 22-23 22.548488420887274 25.729862641898038 29.412757205536792 22.30889173167789 24-25 22.548029487702433 25.87550250735629 29.379221189032922 22.197246815908354 26-27 22.29248330789272 26.00358943468767 29.43089247654635 22.273034780873264 28-29 22.01830559961436 26.20510167616531 29.552233705584275 22.224359018636054 30-31 22.22367230490319 25.811863526296523 29.463199991566093 22.501264177234194 32-33 22.081739681113234 25.980630059289133 29.43615157572988 22.501478683867752 34-35 22.594624895846817 26.010049600909714 29.06528241690648 22.330043086336993 36-37 22.52711817701286 25.806674427008314 29.217940785345604 22.44826661063322 38-39 22.28219261146386 26.010378064288158 29.359837603724298 22.34759172052369 40-41 22.442369123351945 25.933394625953245 29.23145380205101 22.3927824486438 42-43 22.295230596191836 26.002589767582535 28.91880475173639 22.78337488448924 44-45 22.438436726721626 26.03955331933291 28.830889438937294 22.691120515008166 46-47 22.61687773959182 26.34530362438592 28.467544402380923 22.57027423364133 48-49 22.68828230959294 26.268953280533818 28.274799348142743 22.767965061730504 50-51 22.38326388184559 26.419484077778392 28.47409210492355 22.72315993545247 52-53 22.72492294806274 26.63175972155877 27.91032383262407 22.732993497754425 54-55 22.538501725629146 26.400508108984578 28.05757017310098 23.003419992285295 56-57 22.59196445937422 26.750028629544598 27.951197078514028 22.706809832567153 58-59 22.824420626492987 26.416029239780965 27.9567234373756 22.802826696350444 60-61 22.65636047765791 26.182775509580896 28.620722623170604 22.54014138959059 62-63 22.47461876339227 26.369910573994403 28.489042969417454 22.666427693195875 64-65 22.455261896080582 26.527649887688977 28.244868936540545 22.7722192796899 66-67 22.33817825370532 26.737255905746572 27.877465302875393 23.047100537672723 68-69 22.220573592294304 26.526697985786384 28.30950941864702 22.94321900327229 70-71 22.423136082041523 26.436430440122834 28.131541111871268 23.00889236596438 72-73 22.520293874939565 26.378838386959576 28.124638745396847 22.97622899270402 74-75 22.28471397902628 26.81233264923644 27.888867277324643 23.01408609441264 76-77 22.507209106070846 26.591648090182275 27.768447621585835 23.132695182161047 78-79 22.46009332313782 26.62323755585444 27.861934406189448 23.05473471481829 80-81 22.52594627039415 26.711980938176396 27.781261936375707 22.98081085505375 82-83 22.43735866633747 26.601545157139217 28.103286340591264 22.85780983593205 84-85 22.39143270150864 26.496645037068923 27.78725870298035 23.324663558442086 86-87 22.44953170674592 26.42292460654967 28.020434218042805 23.107109468661605 88-89 22.465397816911192 26.32351668302007 27.86809529247382 23.342990207594916 90-91 22.296163786849004 26.41701902959849 28.120362110417275 23.166455073135232 92-93 22.479962731170932 26.307582031973077 28.142257244383217 23.070197992472774 94-95 22.266343043953743 26.394568606463924 28.070246876236126 23.268841473346207 96-97 22.01125784329623 26.56369640035514 28.137627534232728 23.287418222115896 98-99 22.145802725937134 26.484319315751957 27.743356073719582 23.626521884591327 100-101 22.015027178282402 26.40938911253504 28.06871583616054 23.506867873022017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 170.0 1 129.0 2 121.0 3 157.0 4 149.0 5 162.0 6 249.0 7 556.5 8 1035.5 9 1247.0 10 1156.0 11 1117.5 12 1190.5 13 1300.0 14 1424.0 15 1532.5 16 1625.5 17 1687.0 18 1792.5 19 1902.5 20 2069.0 21 2430.0 22 2879.5 23 3418.0 24 4140.5 25 5230.0 26 6800.0 27 8689.0 28 10413.0 29 12405.5 30 15060.5 31 17749.5 32 20219.5 33 22940.5 34 25987.0 35 28249.0 36 31156.0 37 34404.5 38 36219.0 39 37222.5 40 38411.5 41 41128.5 42 43828.5 43 46169.5 44 48815.5 45 50712.5 46 51833.5 47 54240.5 48 59633.0 49 59372.5 50 53912.5 51 50539.5 52 48408.5 53 47582.5 54 46349.5 55 43299.0 56 39580.5 57 35827.0 58 32566.5 59 29502.0 60 25337.0 61 20352.0 62 15585.5 63 11997.5 64 9067.5 65 6811.5 66 5199.5 67 4005.5 68 2876.5 69 1899.0 70 1304.0 71 903.0 72 648.5 73 453.0 74 330.5 75 240.5 76 159.5 77 85.5 78 45.5 79 28.5 80 24.5 81 16.5 82 12.0 83 10.0 84 7.5 85 5.5 86 2.5 87 2.0 88 4.5 89 5.5 90 5.0 91 6.0 92 3.5 93 0.5 94 0.5 95 2.5 96 3.0 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03169853241611158 2 0.02450092987208624 3 0.021229292352074727 4 0.04449427027215662 5 0.08368121745629456 6 0.10760052288037875 7 0.05394566755218989 8 0.07975525243228074 9 0.06659599929623443 10-11 0.06819546652824005 12-13 0.06575991415223148 14-15 0.05907123300020793 16-17 0.04863834446417121 18-19 0.05598135312019706 20-21 0.05281877018418592 22-23 0.03191664158411235 24-25 0.007633820880026871 26-27 0.0023628493200083174 28-29 0.0062888143440221365 30-31 0.004180425720014715 32-33 0.005779892952020345 34-35 0.005816244480020473 36-37 0.006470571984022776 38-39 0.004144074192014588 40-41 0.006434220456022648 42-43 0.003271637520011516 44-45 0.001708521816006014 46-47 0.001817576400006398 48-49 2.5446069600089567E-4 50-51 0.002726364600009597 52-53 0.006325165872022265 54-55 0.011814246600041586 56-57 0.009487748808033396 58-59 0.005198268504018297 60-61 0.002799067656009853 62-63 0.009524100336033525 64-65 0.002653661544009341 66-67 0.01977523123206961 68-69 0.042095069424148174 70-71 0.053763909912189246 72-73 0.0031262314080110045 74-75 0.012032355768042353 76-77 0.020902128600073577 78-79 0.024973499736087906 80-81 0.07146710404825156 82-83 0.028172434200099166 84-85 0.013231956192046576 86-87 0.02991730754410531 88-89 0.0448577855521579 90-91 0.041695202616146766 92-93 0.04234953012014907 94-95 0.014104392864049646 96-97 0.017376030384061162 98-99 0.037660183008132564 100-101 0.07379360184025975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375458.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.827080024442104 #Duplication Level Percentage of deduplicated Percentage of total 1 80.19753517427796 47.97984354634538 2 11.379076316693368 13.615538188060963 3 3.489665612785542 6.263295116239933 4 1.5291448963265024 3.6593709632597156 5 0.8340098586229768 2.494818727650524 6 0.5173846238495178 1.8572166776676586 7 0.34540299963272136 1.4465117029796417 8 0.2586275441397183 1.2378344623817488 9 0.1998821486610416 1.0762528773061413 >10 1.1393164179744382 12.551313181549387 >50 0.07861623463317595 3.2048418536222854 >100 0.029759140692397275 3.0935948116560104 >500 7.287838664894839E-4 0.29452482728517854 >1k 7.287838664894839E-4 0.8593759209164871 >5k 1.2146397774824731E-4 0.3656671430789584 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5032 0.36584177779328775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3571 0.25962261297691386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2247 0.16336376683257506 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2041 0.14838693729652233 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1656 0.12039626073642379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.270305600025592E-5 0.0 0.0 0.0 0.0 12-13 7.270305600025592E-5 0.0 0.0 0.0 0.0 14-15 7.270305600025592E-5 0.0 0.0 3.635152800012796E-5 0.0 16-17 7.270305600025592E-5 0.0 0.0 7.270305600025592E-5 0.0 18-19 1.0905458400038388E-4 0.0 0.0 1.4540611200051183E-4 0.0 20-21 1.4540611200051183E-4 7.270305600025592E-5 0.0 2.1810916800076775E-4 0.0 22-23 1.4540611200051183E-4 7.270305600025592E-5 0.0 4.362183360015355E-4 0.0 24-25 2.9081222400102367E-4 7.270305600025592E-5 0.0 8.36085144002943E-4 0.0 26-27 2.9081222400102367E-4 7.270305600025592E-5 0.0 0.0011268973680039667 0.0 28-29 2.9081222400102367E-4 7.270305600025592E-5 0.0 0.0029444737680103645 0.0 30-31 2.9081222400102367E-4 1.0905458400038388E-4 0.0 0.005307323088018682 0.0 32-33 2.9081222400102367E-4 1.4540611200051183E-4 0.0 0.008106390744028535 0.0 34-35 3.6351528000127956E-4 1.4540611200051183E-4 0.0 0.010723700760037747 0.0 36-37 5.089213920017914E-4 1.4540611200051183E-4 0.0 0.01570386009605528 0.0 38-39 5.089213920017914E-4 1.4540611200051183E-4 0.0 0.020938480128073703 0.0 40-41 5.089213920017914E-4 1.4540611200051183E-4 0.0 0.028317840312099676 0.0 42-43 5.089213920017914E-4 1.4540611200051183E-4 0.0 0.038205455928134485 0.0 44-45 5.089213920017914E-4 1.4540611200051183E-4 0.0 0.04740239251216686 0.0 46-47 5.816244480020473E-4 1.4540611200051183E-4 0.0 0.06350611941622354 0.0 48-49 5.816244480020473E-4 1.4540611200051183E-4 0.0 0.08346310828829379 0.0 50-51 5.816244480020473E-4 1.4540611200051183E-4 0.0 0.09113328069632079 0.0 52-53 5.816244480020473E-4 1.4540611200051183E-4 0.0 0.10694619537637645 0.0 54-55 5.816244480020473E-4 1.4540611200051183E-4 0.0 0.13657269069648073 0.0 56-57 6.543275040023032E-4 1.4540611200051183E-4 0.0 0.17870411164862904 0.0 58-59 6.543275040023032E-4 1.4540611200051183E-4 0.0 0.2362485804728316 0.0 60-61 6.543275040023032E-4 1.4540611200051183E-4 0.0 0.30073619114505856 0.0 62-63 6.543275040023032E-4 1.4540611200051183E-4 0.0 0.3646058258412834 0.0 64-65 6.543275040023032E-4 1.4540611200051183E-4 0.0 0.4338554866815272 0.0 66-67 7.270305600025591E-4 1.4540611200051183E-4 0.0 0.5340402978498798 0.0 68-69 7.270305600025591E-4 1.4540611200051183E-4 0.0 0.6532733096902996 0.0 70-71 7.270305600025591E-4 1.4540611200051183E-4 0.0 0.7117265667145053 0.0 72-73 7.270305600025591E-4 1.4540611200051183E-4 0.0 0.7947534566667975 0.0 74-75 7.270305600025591E-4 1.4540611200051183E-4 0.0 0.8859230888911185 0.0 76-77 7.270305600025591E-4 1.4540611200051183E-4 0.0 0.985235463387468 0.0 78-79 7.99733616002815E-4 1.4540611200051183E-4 0.0 1.0893462395798346 0.0 80-81 8.72436672003071E-4 1.4540611200051183E-4 0.0 1.2575811111644266 0.0 82-83 8.72436672003071E-4 1.4540611200051183E-4 0.0 1.4514801615171091 0.0 84-85 8.72436672003071E-4 1.4540611200051183E-4 0.0 1.7564694814381827 0.0 86-87 9.087882000031989E-4 1.4540611200051183E-4 0.0 2.173894077463652 0.0 88-89 9.451397280033269E-4 1.4540611200051183E-4 0.0 2.6157832518332076 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8010 0.0 28.901026 1 GTACATG 5385 0.0 27.011738 1 TACATGG 5440 0.0 26.655144 2 ACATGGG 5495 0.0 25.004042 3 TATCAAC 9535 0.0 24.48166 2 ATCAACG 9520 0.0 24.370417 3 TCAACGC 9540 0.0 24.319324 4 GGTATCA 5640 0.0 24.104815 1 CAACGCA 9645 0.0 24.005281 5 AACGCAG 9785 0.0 23.660961 6 CATGGGG 3135 0.0 22.140905 4 GAGTACT 4520 0.0 22.133167 12-13 ACGCAGA 10560 0.0 21.782286 7 GGATCGC 110 0.0028634334 21.609344 6 CGCAGAG 10655 0.0 21.186642 8 AGAGTAC 7765 0.0 20.83924 10-11 GTACTTT 4960 0.0 20.17121 14-15 GCAGAGT 11015 0.0 19.889442 9 GTGTAGC 555 0.0 19.69934 1 AGTACTT 4850 0.0 19.69628 12-13 >>END_MODULE