FastQCFastQC Report
Thu 26 May 2016
SRR937004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375458
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.362497437217276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38040.27656242502497347No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31560.22945084473680769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.1771046444166234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95650.024.2894631
GTATAGG3850.022.2129271
GAGTACT50650.021.7113412-13
GGTATCA71750.021.1233481
GTACATG54200.021.0380741
GTGTAGG6000.020.5880931
TCAACGC112800.020.5484184
AGTACTT54300.020.51436412-13
TATCAAC112500.020.4772992
CAACGCA113550.020.3708675
ATCAACG113750.020.2932953
TAAGGTG3750.020.2654555
TACATGG54450.020.238282
GTACTTT53750.020.0147214-15
AACGCAG116300.019.9336456
CGGTTAC2650.019.71580528-29
AGAGTAC88250.019.6220910-11
ACATGGG55200.019.3602583
CATGGGG31050.018.2034234
ACGCAGA128500.017.8562647
Produced by FastQC (version 0.11.5)