##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937004_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375458 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20893767748633 33.0 31.0 34.0 31.0 34.0 2 32.342725841138005 33.0 31.0 34.0 31.0 34.0 3 32.470798817557494 34.0 31.0 34.0 31.0 34.0 4 36.114332825866 37.0 35.0 37.0 35.0 37.0 5 35.95353111472688 37.0 35.0 37.0 35.0 37.0 6 36.044366312893594 37.0 35.0 37.0 35.0 37.0 7 36.010862563597 37.0 35.0 37.0 35.0 37.0 8 35.967860160033965 37.0 35.0 37.0 35.0 37.0 9 37.673776298512934 39.0 37.0 39.0 35.0 39.0 10-11 37.580170386882045 39.0 37.0 39.0 35.0 39.0 12-13 37.482007811216334 39.0 37.0 39.0 35.0 39.0 14-15 38.715780125601796 40.0 38.0 41.0 35.0 41.0 16-17 38.719364749777895 40.0 38.0 41.0 34.5 41.0 18-19 38.820206069541925 40.0 38.0 41.0 35.0 41.0 20-21 38.815937673124154 40.0 38.0 41.0 35.0 41.0 22-23 38.71857846622725 40.0 38.0 41.0 34.0 41.0 24-25 38.62426806198371 40.0 38.0 41.0 34.0 41.0 26-27 38.43591698183441 40.0 38.0 41.0 34.0 41.0 28-29 38.27874024506746 40.0 38.0 41.0 34.0 41.0 30-31 38.29742093179145 40.0 38.0 41.0 34.0 41.0 32-33 38.190351141219864 40.0 38.0 41.0 34.0 41.0 34-35 38.07215087628993 40.0 38.0 41.0 33.5 41.0 36-37 37.94404663755637 40.0 37.0 41.0 33.0 41.0 38-39 37.78103875218291 40.0 37.0 41.0 33.0 41.0 40-41 37.42487375114325 40.0 37.0 41.0 32.5 41.0 42-43 37.259784013761234 40.0 37.0 41.0 32.0 41.0 44-45 37.06006399322989 40.0 36.0 41.0 31.5 41.0 46-47 36.71338892208995 39.0 36.0 41.0 31.0 41.0 48-49 36.36034760785135 39.0 36.0 40.5 30.0 41.0 50-51 36.08532903222054 39.0 35.0 40.0 30.0 41.0 52-53 35.82765958684307 39.0 35.0 40.0 29.0 41.0 54-55 35.45230679526383 38.0 35.0 40.0 28.0 41.0 56-57 35.23946241906332 38.0 34.5 40.0 28.0 41.0 58-59 34.901975196625415 38.0 34.0 40.0 27.0 41.0 60-61 34.50453848827082 37.5 34.0 40.0 26.0 41.0 62-63 34.12864878462301 37.0 33.0 39.5 26.0 41.0 64-65 33.80571998563387 36.0 33.0 39.0 26.0 40.0 66-67 33.46815969662469 36.0 33.0 39.0 25.0 40.0 68-69 33.018558545589904 35.5 32.0 38.5 24.5 40.0 70-71 32.566937703659434 35.0 32.0 38.0 23.0 40.0 72-73 32.14841674554948 35.0 31.0 37.0 22.0 39.0 74-75 31.6653591022045 35.0 31.0 37.0 21.5 39.0 76-77 30.22056871238526 33.5 29.5 35.0 20.0 37.5 78-79 30.908163317236877 34.0 30.5 36.0 20.0 37.5 80-81 30.78406101822084 34.0 31.0 35.0 19.5 37.0 82-83 30.453642350402557 34.0 30.5 35.0 18.0 37.0 84-85 30.024390421226965 34.0 30.0 35.0 16.0 36.0 86-87 29.651424834491493 34.0 30.0 35.0 11.0 36.0 88-89 29.29538524622344 34.0 29.0 35.0 7.0 35.5 90-91 28.849213134824907 34.0 29.0 35.0 2.0 35.0 92-93 28.36397985252912 33.0 29.0 35.0 2.0 35.0 94-95 27.822107981486894 33.0 27.5 34.5 2.0 35.0 96-97 27.066419330870154 33.0 26.0 34.0 2.0 35.0 98-99 26.231798789930338 32.0 25.0 34.0 2.0 35.0 100-101 23.06586497006815 28.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.08315070308959349 1101 2 0.15127753534432742 1101 3 0.15229955460932132 1101 4 0.07709677694945327 1101 5 -0.08810873775243522 1101 6 0.07325064305863549 1101 7 0.0696912612025784 1101 8 0.17015819310331892 1101 9 0.10531491480244881 1101 10-11 0.1545691528417663 1101 12-13 0.11663160592289756 1101 14-15 0.28773681942634255 1101 16-17 0.22196182696180955 1101 18-19 0.02342526936094913 1101 20-21 -0.035476264439090244 1101 22-23 -0.050850287731691424 1101 24-25 0.002945634652846252 1101 26-27 -0.04741385423178457 1101 28-29 0.042136627790931414 1101 30-31 0.17182846100957505 1101 32-33 0.20600417141451288 1101 34-35 0.1064734311914961 1101 36-37 0.11541174132088372 1101 38-39 0.26346012207648783 1101 40-41 0.3842750181083616 1101 42-43 0.46087084421311175 1101 44-45 0.2832646410372206 1101 46-47 0.09006475613879417 1101 48-49 0.32959972326836606 1101 50-51 0.2997005943498081 1101 52-53 0.3596699790865827 1101 54-55 0.13638235635954743 1101 56-57 0.03925523545743914 1101 58-59 0.052549007212789434 1101 60-61 0.3606981152824673 1101 62-63 0.23023031021847373 1101 64-65 0.2609550112223289 1101 66-67 0.2388486826229297 1101 68-69 -0.0388209220408271 1101 70-71 -0.09555590406132097 1101 72-73 -0.014197525984421588 1101 74-75 -0.11765556344564843 1101 76-77 -0.172392942599906 1101 78-79 -0.18661863383153232 1101 80-81 -0.07236375939891104 1101 82-83 0.08550903362411688 1101 84-85 -0.19347424887760667 1101 86-87 -0.4239416459218539 1101 88-89 -0.21467241634025314 1101 90-91 0.07560458540164916 1101 92-93 -0.2512444955495141 1101 94-95 -0.44113294167387807 1101 96-97 -0.4218637620840582 1101 98-99 -0.3241281347946021 1101 100-101 -0.07818673259528808 1102 1 -0.016216808010959483 1102 2 0.024213434882234708 1102 3 0.061313504331430124 1102 4 0.041383028994737 1102 5 -0.00849176118488515 1102 6 0.11538004187212181 1102 7 0.05582058974881221 1102 8 0.22303764461724285 1102 9 0.21473315216521627 1102 10-11 0.2674465883548365 1102 12-13 0.2631628715029848 1102 14-15 0.20327725822858866 1102 16-17 0.2055084158214342 1102 18-19 0.2683340306831852 1102 20-21 0.23942071875365656 1102 22-23 0.11445550864720389 1102 24-25 0.10178844179601754 1102 26-27 0.1483540496961524 1102 28-29 -0.014241965458730022 1102 30-31 0.13045443513114918 1102 32-33 0.06821134938138584 1102 34-35 0.28433280634835256 1102 36-37 0.197441017668595 1102 38-39 0.2507871307996581 1102 40-41 0.17633456792951563 1102 42-43 0.27507336111907676 1102 44-45 0.1824813285052258 1102 46-47 0.057981064498250134 1102 48-49 0.14338084580441546 1102 50-51 0.18630682002814325 1102 52-53 0.15667856005619996 1102 54-55 0.2739267334738358 1102 56-57 0.2733930312072772 1102 58-59 0.17575321889789564 1102 60-61 0.19051961973774922 1102 62-63 0.13222966442898354 1102 64-65 0.24624029625640986 1102 66-67 0.2746299791540636 1102 68-69 0.40656302155800006 1102 70-71 0.14969917442050473 1102 72-73 0.053291657878197896 1102 74-75 0.11748333148473478 1102 76-77 0.38193807135650815 1102 78-79 -0.20311407984453567 1102 80-81 0.1613018237021464 1102 82-83 0.14825183206752612 1102 84-85 0.18550651629928439 1102 86-87 0.024760961327718434 1102 88-89 -0.05515237511680127 1102 90-91 0.0560535596671059 1102 92-93 -0.02752298306369383 1102 94-95 0.24187392778630112 1102 96-97 0.40737427324364006 1102 98-99 0.24016326181717673 1102 100-101 0.32657186594114407 1103 1 0.09307218942427653 1103 2 0.13866798950602544 1103 3 0.13763515212256294 1103 4 0.0920660064305423 1103 5 -0.020562738966511063 1103 6 0.08125638549334013 1103 7 0.02081079176028311 1103 8 0.08541192777911277 1103 9 0.1309513447245081 1103 10-11 -0.0157233851329579 1103 12-13 0.03688336947134019 1103 14-15 0.09842617324544989 1103 16-17 0.019993228939213736 1103 18-19 0.025113374217944795 1103 20-21 -0.040484759474637144 1103 22-23 0.07031953273872915 1103 24-25 0.1459352243094827 1103 26-27 -0.08232374056925806 1103 28-29 -0.04997040267956265 1103 30-31 0.15329419479225237 1103 32-33 0.13767800740276215 1103 34-35 0.02319569934854826 1103 36-37 0.07273639820517985 1103 38-39 -0.08186808807010948 1103 40-41 0.22095774161326887 1103 42-43 0.05659262569358958 1103 44-45 -0.02686782569782764 1103 46-47 0.014483578835005062 1103 48-49 9.840128601226183E-4 1103 50-51 -0.06962870776644792 1103 52-53 -0.006839782354681745 1103 54-55 0.1859690737522186 1103 56-57 -0.022569260945694225 1103 58-59 -0.09226409328330476 1103 60-61 -0.3811236686401074 1103 62-63 0.06985664203769915 1103 64-65 -0.05670306272460124 1103 66-67 -0.026875200811922184 1103 68-69 0.04492538863675577 1103 70-71 0.014096994368060223 1103 72-73 0.1348085810216233 1103 74-75 -0.04636551292897195 1103 76-77 0.10633362191700968 1103 78-79 0.0248935784362061 1103 80-81 0.010893694253429231 1103 82-83 0.23074405131193032 1103 84-85 -0.07125301512632376 1103 86-87 -0.29304418117538233 1103 88-89 -0.19516545110884564 1103 90-91 -0.37304340439732897 1103 92-93 -0.33663421184672515 1103 94-95 -0.2656258560815985 1103 96-97 -0.04434992058362752 1103 98-99 -0.44739707506071724 1103 100-101 0.11361420621951979 1104 1 0.06042739551654819 1104 2 0.15746515851193266 1104 3 0.08960600551873199 1104 4 0.11885629431088063 1104 5 -0.10542853140580632 1104 6 0.0016880352560022516 1104 7 0.11609645238248589 1104 8 0.10276841447787888 1104 9 -0.009379649367630805 1104 10-11 0.13969635498930444 1104 12-13 0.18020244538570296 1104 14-15 0.16131035519401848 1104 16-17 0.1572785139168431 1104 18-19 0.15831139013162243 1104 20-21 0.15469674515262 1104 22-23 0.19985501407602158 1104 24-25 0.24375862013641836 1104 26-27 0.18314598589319786 1104 28-29 0.09720543312960928 1104 30-31 0.09065332807777793 1104 32-33 0.15623826420705456 1104 34-35 0.2652121850228255 1104 36-37 0.17139005390984607 1104 38-39 0.18935262872803094 1104 40-41 0.4295781442359541 1104 42-43 0.23309008155535338 1104 44-45 0.3983874418635551 1104 46-47 0.3810582552684352 1104 48-49 0.4546495355183211 1104 50-51 0.5131721066992796 1104 52-53 0.37979239331038883 1104 54-55 0.46373582408516967 1104 56-57 0.4134921910913292 1104 58-59 0.1163627123718527 1104 60-61 0.22600653489094924 1104 62-63 0.2516483385851842 1104 64-65 0.20073299232440434 1104 66-67 0.11704682732726468 1104 68-69 0.22153383039477603 1104 70-71 0.3600475031957018 1104 72-73 0.24129421261675077 1104 74-75 0.08102815404503971 1104 76-77 0.1981125550864249 1104 78-79 0.32572191740420564 1104 80-81 0.40501528357146555 1104 82-83 0.4815060121765917 1104 84-85 0.5164301116024959 1104 86-87 0.4654451727159561 1104 88-89 0.4942842722801508 1104 90-91 0.49833141204906894 1104 92-93 0.423756842690036 1104 94-95 0.43327154172600046 1104 96-97 0.6288617124457758 1104 98-99 0.602336345353855 1104 100-101 0.9296518816626111 1105 1 -0.02885337094735263 1105 2 0.0673016824791759 1105 3 -1.459842319277982E-4 1105 4 0.03374027742183472 1105 5 -0.016250080449886184 1105 6 0.053383740038270844 1105 7 0.028725999067589214 1105 8 0.12658822733582298 1105 9 0.13217893992393925 1105 10-11 0.10391039394799151 1105 12-13 0.07179980018028687 1105 14-15 0.09689384591953143 1105 16-17 -0.15929186390064132 1105 18-19 -0.033970271116352535 1105 20-21 0.09108207806567492 1105 22-23 0.011497565634137175 1105 24-25 0.006062059971085887 1105 26-27 -0.07700294989842149 1105 28-29 -0.14397970455782882 1105 30-31 0.09383368807584702 1105 32-33 0.10066870999762756 1105 34-35 -0.02181030023992747 1105 36-37 0.09563352287584337 1105 38-39 0.04714948351118409 1105 40-41 0.1080565855322746 1105 42-43 0.12705340303448764 1105 44-45 0.05911080614998099 1105 46-47 -0.08218458073950075 1105 48-49 0.005151294582454113 1105 50-51 0.13615312304180094 1105 52-53 0.15486366464094203 1105 54-55 0.3077639347943659 1105 56-57 0.15316388541072 1105 58-59 0.10569171328951654 1105 60-61 0.08496985001509216 1105 62-63 0.16990285508403957 1105 64-65 0.06498430336100824 1105 66-67 0.1496529049955413 1105 68-69 0.3180210085457702 1105 70-71 0.10852691045209895 1105 72-73 0.04877137909996776 1105 74-75 0.009974005627817917 1105 76-77 0.09535124382104243 1105 78-79 0.1765656072700139 1105 80-81 0.050728613312102766 1105 82-83 -0.030054369225116062 1105 84-85 -0.5663901613574822 1105 86-87 -0.0813868913119471 1105 88-89 0.3981534507819475 1105 90-91 0.23323304299639602 1105 92-93 0.07803925509906762 1105 94-95 0.31597078370154463 1105 96-97 0.25015341078152886 1105 98-99 0.058265211291306684 1105 100-101 0.29484357389756255 1106 1 -0.013929280770859975 1106 2 0.09404422580647775 1106 3 0.10562833576329922 1106 4 0.10321676825893178 1106 5 -0.02098374939152592 1106 6 0.0749198444615331 1106 7 0.09572124351115008 1106 8 0.1935568088269619 1106 9 0.2533083348341023 1106 10-11 0.19794107624100832 1106 12-13 0.3495758313799229 1106 14-15 0.48534878751970467 1106 16-17 0.289707877428917 1106 18-19 0.24447651241629131 1106 20-21 0.18197648083543783 1106 22-23 0.1587043229017837 1106 24-25 0.2153190788938275 1106 26-27 0.3018061712190345 1106 28-29 0.2748337940592762 1106 30-31 0.25762659254687037 1106 32-33 0.22727236719961041 1106 34-35 0.30162225641885243 1106 36-37 0.3276368258983098 1106 38-39 0.4647882204035554 1106 40-41 0.4543969958233802 1106 42-43 0.5343926791552249 1106 44-45 0.2358680737942862 1106 46-47 0.3855224053519848 1106 48-49 0.5154360310370762 1106 50-51 0.48678368196891597 1106 52-53 0.457890904627412 1106 54-55 0.5114712790449119 1106 56-57 0.34808698070093413 1106 58-59 0.41957933093481614 1106 60-61 0.22660922363468217 1106 62-63 0.22172011437130124 1106 64-65 0.23342591348497166 1106 66-67 0.24966683340352347 1106 68-69 0.1552080670649829 1106 70-71 0.1457352595378012 1106 72-73 0.0593665200753577 1106 74-75 0.06724682334475318 1106 76-77 0.38588788548290154 1106 78-79 0.4118285557150081 1106 80-81 0.37837431440846814 1106 82-83 0.44868510985242693 1106 84-85 0.5735545229650967 1106 86-87 0.5722000716981803 1106 88-89 0.5664474197812908 1106 90-91 0.3144422277865786 1106 92-93 0.5504243954775205 1106 94-95 0.7362105512961072 1106 96-97 0.8973050513490151 1106 98-99 0.8465627618812732 1106 100-101 1.626012963236363 1107 1 0.09915261578086643 1107 2 0.2858276309788934 1107 3 0.1850733788667469 1107 4 0.11811978550031199 1107 5 0.07640310405674455 1107 6 0.13320648239256627 1107 7 0.1496867607525303 1107 8 0.17328741227523636 1107 9 0.21324906759272722 1107 10-11 0.23428267106859124 1107 12-13 0.2323775555178571 1107 14-15 0.2357717401059105 1107 16-17 0.22209473087719545 1107 18-19 0.2587949391404294 1107 20-21 0.15525773083543726 1107 22-23 0.07208794359143411 1107 24-25 0.11378890648002482 1107 26-27 0.17424421001214085 1107 28-29 0.11583325526617472 1107 30-31 0.1960721744434153 1107 32-33 0.18165814306168215 1107 34-35 0.05659262279816346 1107 36-37 0.07964652417416573 1107 38-39 0.31907970747252534 1107 40-41 0.21071973289234336 1107 42-43 0.05462436018971317 1107 44-45 0.21980126344946171 1107 46-47 0.2598354441450894 1107 48-49 0.24601792758880237 1107 50-51 0.09121794921029647 1107 52-53 0.35400081842051634 1107 54-55 0.4280203092173309 1107 56-57 0.32556542897679464 1107 58-59 0.3400669386934396 1107 60-61 0.21122129260020017 1107 62-63 0.13322873506096045 1107 64-65 0.12259078417462632 1107 66-67 0.4347557342655932 1107 68-69 0.056080911892571805 1107 70-71 0.08597502246882982 1107 72-73 0.33353677869605036 1107 74-75 0.34926190955165026 1107 76-77 -0.10473711451709988 1107 78-79 0.3713355600253543 1107 80-81 0.3322024394084693 1107 82-83 0.4788817693351852 1107 84-85 0.3789559238271636 1107 86-87 0.6646381104912855 1107 88-89 0.7346855663330167 1107 90-91 0.587763887269336 1107 92-93 0.6820219170292425 1107 94-95 0.6982822107788635 1107 96-97 0.6249451375559119 1107 98-99 0.7150514472261023 1107 100-101 1.42910294168464 1108 1 0.11737130072187796 1108 2 0.18871028625300568 1108 3 0.14986725031018722 1108 4 0.11677631742258399 1108 5 0.024801026506438006 1108 6 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2316 64-65 -1.0502154658253673 2316 66-67 -1.154884890734408 2316 68-69 -0.9494815881074281 2316 70-71 -0.7873406025311667 2316 72-73 -1.0273507213039537 2316 74-75 -0.6503255904483503 2316 76-77 -0.6041121145171005 2316 78-79 -0.6121300649746466 2316 80-81 -0.8812256855915308 2316 82-83 -0.9357838556648161 2316 84-85 -0.8421972011728336 2316 86-87 -0.6914275145087139 2316 88-89 -1.346145683666986 2316 90-91 -1.3860267377306634 2316 92-93 -1.2685687079707542 2316 94-95 -1.2982584142211344 2316 96-97 -0.7434673624440933 2316 98-99 -0.9016579277739005 2316 100-101 -1.5809939333153586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 11.0 5 42.0 6 60.0 7 163.0 8 332.0 9 709.0 10 1110.0 11 1832.0 12 2843.0 13 3854.0 14 5025.0 15 6999.0 16 9183.0 17 12621.0 18 11371.0 19 7242.0 20 6693.0 21 6925.0 22 7257.0 23 7871.0 24 9251.0 25 11005.0 26 13509.0 27 16911.0 28 20951.0 29 26624.0 30 33398.0 31 41858.0 32 53523.0 33 71386.0 34 99294.0 35 143695.0 36 222961.0 37 318644.0 38 191294.0 39 9009.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90122020390058 19.564566093165986 14.87412556901642 22.660088133917014 2 15.555595947404965 21.027689824525687 39.32965686720008 24.087057360869267 3 19.72491871739244 24.81249745537454 29.544337554018764 25.91824627321426 4 13.319199859246883 17.26428578698877 36.89207522148986 32.52443913227449 5 14.186192089886138 37.44875235290226 33.50074776669679 14.864307790514813 6 31.915914553552344 36.34018632339192 17.206050639132563 14.537848483923174 7 28.45267314984994 32.799377079310425 20.63621082753647 18.111738943303166 8 26.544758182365435 34.64293348106594 19.60772339104502 19.2045849455236 9 26.6197877361577 16.953771034811677 20.158230931078958 36.26821029795166 10-11 24.87996725454358 26.825646439222428 27.17051338535964 21.123872920874355 12-13 26.16179646204294 24.11816456662571 27.630237681496617 22.089801289834735 14-15 22.88279864898123 25.496098789703264 26.152013968234115 25.46908859308139 16-17 22.245499317318306 28.85177155536556 26.49906431166928 22.403664815646863 18-19 22.159773950261354 27.36170975379321 29.02701989422367 21.451496401721766 20-21 22.97985819892864 26.383730973471913 28.986555653139924 21.64985517445952 22-23 22.636673224596745 26.381491223860976 28.896479220376985 22.08535633116529 24-25 22.74506026198651 26.501605835170604 28.941457447639955 21.811876455202924 26-27 22.619989298094595 26.57002477723751 28.952034816090084 21.85795110857781 28-29 22.12296217507582 26.852449970209868 29.11362817626597 21.91095967844834 30-31 22.43995179785635 26.44952679381224 28.92699338220192 22.183528026129487 32-33 22.396301738258632 26.723009152594372 28.85575485093318 22.024934258213815 34-35 22.6986574653679 26.602593463413644 28.775287940453286 21.92346113076517 36-37 22.808227369771057 26.3632883741744 28.803682620788283 22.024801635266265 38-39 22.55242943879315 26.65317190515713 28.853777787957014 21.940620868092704 40-41 22.64747248448964 26.622494504531787 28.649465284671976 22.080567726306597 42-43 22.555614202687398 26.808997439398368 28.291848969579586 22.343539388334648 44-45 22.719519864480223 26.754541257556646 28.198837467146998 22.327101410816137 46-47 22.97337971798423 27.108265991980833 27.71748257849221 22.200871711542725 48-49 23.09365316861729 27.191306459375713 27.443695118280605 22.271345253726395 50-51 22.768038417007013 27.296868439563553 27.581978759408404 22.353114384021026 52-53 23.145283771964582 27.555830697232114 26.992670778281774 22.306214752521534 54-55 22.76696198648014 27.340747590984236 27.22449540443983 22.66779501809579 56-57 22.8023817573094 27.60653747305437 27.21844771366566 22.37263305597057 58-59 23.1033730656401 27.207033308977756 27.207614934694334 22.48197869068781 60-61 22.93290932032503 27.122945959789284 27.802806323406614 22.141338396479064 62-63 22.740070122123 27.233520483184687 27.68853272456619 22.33787667012612 64-65 22.670275162991224 27.435744106960776 27.44508645305289 22.44889427699511 66-67 22.654607648727787 27.529603786376533 27.23075776605239 22.585030798843295 68-69 22.543303852179825 27.32346750711583 27.446772159031013 22.686456481673336 70-71 22.73298161724211 27.349337106168797 27.383471476353314 22.53420980023578 72-73 22.819902017414684 27.250638609878884 27.31080080424676 22.618658568459672 74-75 22.678095530303224 27.7005379864062 26.942798365428693 22.678568117861882 76-77 22.81703462921778 27.537102214027776 26.95587721026539 22.68998594648906 78-79 22.76844266466587 27.571663714909718 26.83187054146564 22.828023078958772 80-81 22.790582360720624 27.65451836858441 26.88569810363242 22.669201167062546 82-83 22.743386379944994 27.759646430241002 26.995972974800374 22.500994215013627 84-85 22.6749332475412 27.668653336168838 26.787248235535756 22.86916518075421 86-87 22.585008542658766 27.479443091351925 26.98720418772038 22.948344178268933 88-89 22.62704864993769 27.506583453175686 26.742161292058125 23.124206604828494 90-91 22.583842992872036 27.497259035005833 26.915076616151485 23.00382135597065 92-93 22.670375976583802 27.461616421584665 26.937899957686824 22.930107644144716 94-95 22.34873765683867 27.53908152775294 26.99947944611904 23.112701369289358 96-97 22.38800218982719 27.550563415199814 26.997290343312848 23.064144051660147 98-99 22.33744583102953 27.658137405334944 26.520284577715593 23.48413218591994 100-101 21.955855778238483 27.867889417332186 26.57948624550911 23.596768558920218 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 219.0 1 173.5 2 129.0 3 153.5 4 163.5 5 163.0 6 261.5 7 512.5 8 899.0 9 1117.0 10 1100.5 11 1139.5 12 1235.0 13 1345.0 14 1513.0 15 1650.0 16 1768.0 17 1939.5 18 2060.5 19 2144.5 20 2353.5 21 2655.5 22 3074.0 23 3652.5 24 4354.5 25 5438.5 26 6992.0 27 8855.5 28 10521.0 29 12511.0 30 15182.5 31 17836.0 32 20384.5 33 22961.0 34 26380.0 35 28706.5 36 31187.0 37 34460.5 38 36096.0 39 37129.0 40 38235.5 41 40820.5 42 43296.5 43 45564.0 44 48439.0 45 50409.5 46 51514.0 47 54223.5 48 60195.5 49 59438.0 50 53822.5 51 51120.5 52 48631.0 53 46744.5 54 45118.5 55 42484.0 56 38792.5 57 35126.5 58 32585.5 59 29578.0 60 25251.0 61 20453.5 62 16015.5 63 12353.0 64 9028.5 65 6873.0 66 5320.0 67 4201.0 68 3027.0 69 1946.5 70 1336.5 71 911.5 72 736.0 73 512.0 74 370.0 75 295.0 76 182.5 77 94.0 78 44.5 79 26.5 80 14.5 81 4.0 82 2.5 83 1.5 84 2.0 85 3.5 86 3.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020211449568071145 2 0.0031262314080110045 3 7.270305600025591E-4 4 0.0 5 0.0021083886240074214 6 0.0 7 1.4540611200051183E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.00639786892802252 14-15 0.0037442073840131796 16-17 0.0 18-19 6.179759760021753E-4 20-21 7.270305600025591E-4 22-23 0.001744873344006142 24-25 3.998668080014075E-4 26-27 1.4540611200051183E-4 28-29 1.8175764000063978E-4 30-31 3.635152800012796E-5 32-33 7.270305600025592E-5 34-35 0.0 36-37 0.0017812248720062698 38-39 0.007960984632028023 40-41 4.7256986400166345E-4 42-43 0.0 44-45 9.451397280033269E-4 46-47 2.1810916800076775E-4 48-49 0.0 50-51 0.0018539279280065258 52-53 3.6351528000127956E-4 54-55 0.0 56-57 2.1810916800076775E-4 58-59 2.1810916800076775E-4 60-61 0.002072037096007294 62-63 3.635152800012796E-5 64-65 3.635152800012796E-5 66-67 3.635152800012796E-5 68-69 2.1810916800076775E-4 70-71 8.360851440029431E-4 72-73 6.906790320024311E-4 74-75 0.0037442073840131796 76-77 7.270305600025592E-5 78-79 4.362183360015355E-4 80-81 0.0028354191840099805 82-83 7.270305600025592E-5 84-85 0.0029444737680103645 86-87 5.816244480020473E-4 88-89 0.0029081222400102365 90-91 0.0021810916800076774 92-93 0.0 94-95 0.0 96-97 5.089213920017913E-4 98-99 0.0011996004240042227 100-101 0.002508255432008829 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375458.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.770298350559656 #Duplication Level Percentage of deduplicated Percentage of total 1 79.02827921063744 45.654872681298556 2 11.843556481288923 13.684115829115312 3 3.7387619747352074 6.479681842265417 4 1.6934912859360676 3.913339873703983 5 0.9153025859233287 2.6438651734914735 6 0.5834704607372775 2.0224357557318613 7 0.39007561039325667 1.5774349074186578 8 0.26714585315938943 1.2346476512106184 9 0.21761149588295642 1.131433293750298 >10 1.1943326656840882 12.67300627202285 >50 0.08662427494357493 3.3873668250160054 >100 0.03920508876241912 3.8567006702215334 >500 0.001386654769273661 0.5240484365620668 >1k 7.563571468765423E-4 1.2170507881913926 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4986 0.362497437217276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3804 0.27656242502497347 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3156 0.22945084473680769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2436 0.1771046444166234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.270305600025592E-5 0.0 0.0 0.0 0.0 7 7.270305600025592E-5 0.0 0.0 0.0 0.0 8 7.270305600025592E-5 0.0 0.0 0.0 0.0 9 7.270305600025592E-5 0.0 0.0 0.0 0.0 10-11 7.270305600025592E-5 0.0 0.0 0.0 0.0 12-13 7.270305600025592E-5 0.0 0.0 0.0 0.0 14-15 7.270305600025592E-5 0.0 0.0 0.0 0.0 16-17 1.4540611200051183E-4 0.0 0.0 0.0 0.0 18-19 1.4540611200051183E-4 0.0 0.0 7.270305600025592E-5 0.0 20-21 1.4540611200051183E-4 0.0 0.0 1.4540611200051183E-4 0.0 22-23 1.4540611200051183E-4 0.0 0.0 3.271637520011516E-4 0.0 24-25 1.4540611200051183E-4 0.0 0.0 6.906790320024311E-4 0.0 26-27 1.4540611200051183E-4 0.0 0.0 0.0010178427840035827 0.0 28-29 2.544606960008957E-4 0.0 0.0 0.0028354191840099805 0.0 30-31 2.9081222400102367E-4 0.0 0.0 0.005234620032018426 0.0 32-33 2.9081222400102367E-4 0.0 0.0 0.00817909380002879 0.0 34-35 3.9986680800140756E-4 0.0 0.0 0.01083275534403813 0.0 36-37 5.089213920017914E-4 0.0 0.0 0.015812914680055662 0.0 38-39 6.543275040023032E-4 0.0 0.0 0.021120237768074344 0.0 40-41 6.906790320024311E-4 0.0 0.0 0.028535949480100447 0.0 42-43 7.270305600025591E-4 0.0 0.0 0.038387213568135126 0.0 44-45 7.270305600025591E-4 0.0 0.0 0.04758415015216749 0.0 46-47 8.36085144002943E-4 0.0 0.0 0.06339706483222315 0.0 48-49 8.72436672003071E-4 0.0 0.0 0.08335405370429341 0.0 50-51 8.72436672003071E-4 0.0 0.0 0.09113328069632079 0.0 52-53 8.72436672003071E-4 0.0 0.0 0.10672808620837568 0.0 54-55 8.72436672003071E-4 0.0 0.0 0.136645393752481 0.0 56-57 0.001017842784003583 0.0 0.0 0.17899492387263005 0.0 58-59 0.001017842784003583 0.0 0.0 0.23632128352883186 0.0 60-61 0.001017842784003583 0.0 0.0 0.3006998396170585 0.0 62-63 0.001017842784003583 0.0 0.0 0.3647512319532839 0.0 64-65 0.001017842784003583 7.270305600025592E-5 0.0 0.4339645412655275 0.0 66-67 0.001017842784003583 7.270305600025592E-5 0.0 0.5341130009058801 0.0 68-69 0.001017842784003583 7.270305600025592E-5 0.0 0.6539276371943018 0.0 70-71 0.0011632488960040947 7.270305600025592E-5 0.0 0.7131442763065103 0.0 72-73 0.0012359519520043505 7.270305600025592E-5 0.0 0.7967891422348048 0.0 74-75 0.0012359519520043505 7.270305600025592E-5 0.0 0.8885403989071277 0.0 76-77 0.0012359519520043505 7.270305600025592E-5 0.0 0.9875983127074763 0.0 78-79 0.0012359519520043505 7.270305600025592E-5 0.0 1.0918908465398434 0.0 80-81 0.0013086550080046065 7.270305600025592E-5 0.0 1.2604165303484367 0.0 82-83 0.0013086550080046065 7.270305600025592E-5 0.0 1.4540611200051181 0.0 84-85 0.0013086550080046065 7.270305600025592E-5 0.0 1.7593049006221926 0.0 86-87 0.0013450065360047345 7.270305600025592E-5 0.0 2.174148538159653 0.0 88-89 0.0013813580640048625 7.270305600025592E-5 0.0 2.6152379789132056 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9565 0.0 24.289463 1 GTATAGG 385 0.0 22.212927 1 GAGTACT 5065 0.0 21.71134 12-13 GGTATCA 7175 0.0 21.123348 1 GTACATG 5420 0.0 21.038074 1 GTGTAGG 600 0.0 20.588093 1 TCAACGC 11280 0.0 20.548418 4 AGTACTT 5430 0.0 20.514364 12-13 TATCAAC 11250 0.0 20.477299 2 CAACGCA 11355 0.0 20.370867 5 ATCAACG 11375 0.0 20.293295 3 TAAGGTG 375 0.0 20.265455 5 TACATGG 5445 0.0 20.23828 2 GTACTTT 5375 0.0 20.01472 14-15 AACGCAG 11630 0.0 19.933645 6 CGGTTAC 265 0.0 19.715805 28-29 AGAGTAC 8825 0.0 19.62209 10-11 ACATGGG 5520 0.0 19.360258 3 CATGGGG 3105 0.0 18.203423 4 ACGCAGA 12850 0.0 17.856264 7 >>END_MODULE