##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937003_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38234 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.9476382277554 31.0 31.0 34.0 28.0 34.0 2 30.94531045666161 31.0 31.0 34.0 27.0 34.0 3 30.755191714181095 31.0 31.0 34.0 27.0 34.0 4 34.36025527017838 37.0 35.0 37.0 32.0 37.0 5 34.38196369723283 37.0 35.0 37.0 32.0 37.0 6 34.55194329654235 37.0 35.0 37.0 32.0 37.0 7 34.533190354135066 37.0 35.0 37.0 32.0 37.0 8 34.4948213631846 37.0 35.0 37.0 31.0 37.0 9 35.93327928022179 39.0 37.0 39.0 31.0 39.0 10-11 35.80441491865878 39.0 36.0 39.0 30.0 39.0 12-13 35.66236857247476 39.0 35.0 39.0 30.0 39.0 14-15 36.409203849976464 39.0 36.0 41.0 29.0 41.0 16-17 36.323808651985146 39.0 36.0 40.5 29.5 41.0 18-19 36.16093006224826 39.0 35.0 41.0 30.0 41.0 20-21 36.15675838259141 39.0 35.0 41.0 30.0 41.0 22-23 35.96042789140556 39.0 35.0 41.0 30.0 41.0 24-25 35.68050687869436 38.5 35.0 41.0 28.0 41.0 26-27 35.42463514149709 38.0 35.0 40.5 28.0 41.0 28-29 35.219202803787205 38.0 35.0 40.0 27.0 41.0 30-31 34.8144452581472 37.0 35.0 40.0 27.0 41.0 32-33 34.231783229586235 35.0 35.0 40.0 25.5 41.0 34-35 33.845674007427945 35.0 35.0 40.0 24.5 41.0 36-37 33.476722289062096 35.0 34.0 39.0 23.0 41.0 38-39 33.103376575822566 35.0 33.0 39.0 21.0 41.0 40-41 32.68870638698541 35.0 33.0 39.0 16.5 41.0 42-43 32.127229690851074 35.0 33.0 39.0 8.5 41.0 44-45 31.48592875451169 35.0 33.0 39.0 2.0 41.0 46-47 30.60733901762829 35.0 31.0 38.0 2.0 40.0 48-49 29.85163728618507 35.0 30.0 38.0 2.0 40.0 50-51 28.98782497253753 35.0 27.5 37.5 2.0 39.5 52-53 29.037218182769266 35.0 28.0 38.0 2.0 40.0 54-55 29.05028247109902 35.0 27.5 38.0 2.0 40.5 56-57 28.632460637129256 35.0 26.5 38.0 2.0 40.5 58-59 28.244455196945125 35.0 25.0 38.0 2.0 40.0 60-61 27.691910341580794 35.0 22.0 37.5 2.0 40.0 62-63 27.258042579902707 35.0 20.5 37.0 2.0 40.0 64-65 26.83988073442486 35.0 15.5 36.5 2.0 39.5 66-67 26.190694146571115 35.0 5.0 36.0 2.0 39.0 68-69 25.621227179996865 34.0 2.0 35.0 2.0 39.0 70-71 25.04456766229011 33.5 2.0 35.0 2.0 38.0 72-73 24.53226186117069 33.0 2.0 35.0 2.0 37.0 74-75 24.056598838729926 33.0 2.0 35.0 2.0 37.0 76-77 23.607587487576502 33.0 2.0 35.0 2.0 36.0 78-79 23.046947742846683 32.5 2.0 35.0 2.0 36.0 80-81 22.423275095464767 31.5 2.0 35.0 2.0 35.5 82-83 21.88057749646911 31.0 2.0 35.0 2.0 35.0 84-85 21.354671235026416 31.0 2.0 35.0 2.0 35.0 86-87 20.987380342103886 30.5 2.0 35.0 2.0 35.0 88-89 20.362033791912957 29.5 2.0 35.0 2.0 35.0 90-91 19.738361144531048 29.0 2.0 35.0 2.0 35.0 92-93 19.130407490715072 27.0 2.0 34.0 2.0 35.0 94-95 18.521185332426636 25.0 2.0 34.0 2.0 35.0 96-97 17.863053826437202 22.0 2.0 34.0 2.0 35.0 98-99 17.232437097871006 10.5 2.0 34.0 2.0 35.0 100-101 15.891889417795678 2.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9725557066848403 1101 2 1.4798622315108219 1101 3 1.0738556614114785 1101 4 1.2659278017390392 1101 5 0.7638279793133691 1101 6 1.0390231015872047 1101 7 1.2366734085855953 1101 8 1.5623584651923466 1101 9 0.8396472542304494 1101 10-11 1.250102888468227 1101 12-13 1.1004518512554213 1101 14-15 2.1677662214017417 1101 16-17 1.84251701700601 1101 18-19 2.3094357506753838 1101 20-21 1.885872825603954 1101 22-23 2.6346293572091213 1101 24-25 2.4131839271042637 1101 26-27 1.8537584628382646 1101 28-29 2.086418530392258 1101 30-31 2.1057146339526724 1101 32-33 1.9571858094978367 1101 34-35 1.9549369880829275 1101 36-37 2.3407407000441722 1101 38-39 2.4647163858148886 1101 40-41 2.6128111619677554 1101 42-43 2.750245799509109 1101 44-45 3.311111268653022 1101 46-47 4.175574166112231 1101 48-49 3.5802176935257073 1101 50-51 2.691171293483432 1101 52-53 3.1413638926962584 1101 54-55 2.387217472363993 1101 56-57 2.7011951367938174 1101 58-59 3.5921647480682566 1101 60-61 3.8033518210093824 1101 62-63 4.070824683901126 1101 64-65 3.130808586993048 1101 66-67 3.678693822027899 1101 68-69 3.281363539664401 1101 70-71 2.4757869748075265 1101 72-73 3.448142254761727 1101 74-75 3.70376116444535 1101 76-77 3.775390711254758 1101 78-79 3.5224037053998636 1101 80-81 2.956214014940702 1101 82-83 2.570924589229403 1101 84-85 2.439183111172593 1101 86-87 1.1302516726471659 1101 88-89 2.577514913240279 1101 90-91 3.0540274625270705 1101 92-93 3.711884114098609 1101 94-95 3.1835323148565315 1101 96-97 3.39578998634855 1101 98-99 2.937830934590238 1101 100-101 2.770094413291673 1102 1 -0.415957806828672 1102 2 -0.7289215522729577 1102 3 -1.457901095345278 1102 4 0.2605223963336343 1102 5 -1.5213071558217663 1102 6 -1.5028687903046887 1102 7 -0.9937319968198111 1102 8 -1.1302091023752183 1102 9 -0.2306230160398215 1102 10-11 0.14604883441418082 1102 12-13 -0.8387373379337717 1102 14-15 -0.8021662110306949 1102 16-17 -1.659847847858856 1102 18-19 -2.322321006081374 1102 20-21 -1.5972352825041582 1102 22-23 -1.6106409130611539 1102 24-25 -1.2885052620849322 1102 26-27 -1.7516469425671417 1102 28-29 -0.7862166047428758 1102 30-31 -2.0290826633446244 1102 32-33 -1.805989866177839 1102 34-35 -1.3633062551603174 1102 36-37 -1.4163538945504186 1102 38-39 -1.6538646952661864 1102 40-41 -1.6341482974916985 1102 42-43 -0.8848893356260277 1102 44-45 -0.12199683945508966 1102 46-47 -0.3254393474012822 1102 48-49 0.33258255839057327 1102 50-51 -1.9230178957057547 1102 52-53 -1.0103252964929368 1102 54-55 -0.5428500952035726 1102 56-57 0.03768162328030655 1102 58-59 -0.3852001167966037 1102 60-61 -1.1513779087203488 1102 62-63 -0.6497834242069764 1102 64-65 -0.18473195354749095 1102 66-67 -1.3831305022964244 1102 68-69 -3.3645824062815457 1102 70-71 -2.0965103224897703 1102 72-73 -2.1677361236166526 1102 74-75 -1.867184781500594 1102 76-77 -2.321906586042541 1102 78-79 -2.3076638621677006 1102 80-81 -1.9806103093836178 1102 82-83 -3.0956294648246505 1102 84-85 -3.188857429367946 1102 86-87 -2.8312348138393197 1102 88-89 -3.58228238405702 1102 90-91 -3.8621887536891464 1102 92-93 -4.9185212913067975 1102 94-95 -3.7347109283867095 1102 96-97 -3.623466770408209 1102 98-99 -4.35946636270706 1102 100-101 -3.4981488299515675 1103 1 -0.1332135240843897 1103 2 -1.7143685377199454 1103 3 -0.7011443385885201 1103 4 -1.5135593777481446 1103 5 -1.9502745847891916 1103 6 -0.8673871548230494 1103 7 -0.9729419760297944 1103 8 -0.28059025275636884 1103 9 -2.652660438077241 1103 10-11 -1.6460509576856168 1103 12-13 -1.6367276359240677 1103 14-15 -2.485118393982873 1103 16-17 -2.1795983676093797 1103 18-19 -1.7065898903502585 1103 20-21 -1.8096399949088635 1103 22-23 -2.0839603863806246 1103 24-25 -0.7275212011008634 1103 26-27 -0.8590620499822492 1103 28-29 -0.9074917260179944 1103 30-31 -1.3215289557909173 1103 32-33 -0.30980136998934427 1103 34-35 -0.49185788371195116 1103 36-37 -0.8474003255968441 1103 38-39 -1.175347716749208 1103 40-41 -0.7772529405963411 1103 42-43 0.4297329789962916 1103 44-45 0.19123947378122352 1103 46-47 0.9829459609840292 1103 48-49 1.6215638473718634 1103 50-51 1.2148892422013837 1103 52-53 -0.07754636371400281 1103 54-55 -1.3342568866103655 1103 56-57 -1.4231638375651556 1103 58-59 -1.0459762775727661 1103 60-61 -1.2678020251444657 1103 62-63 -1.1185983930219479 1103 64-65 -1.0929093617248995 1103 66-67 -1.8152164343823571 1103 68-69 -0.8032518449509887 1103 70-71 -0.35049507647452316 1103 72-73 -0.21243466831519697 1103 74-75 -0.04944396375977789 1103 76-77 -0.3066605707965273 1103 78-79 0.6573396028357621 1103 80-81 0.4719191431458363 1103 82-83 1.3430399738447925 1103 84-85 1.3645036239931052 1103 86-87 1.5398670572625512 1103 88-89 1.663732861958227 1103 90-91 1.9309505394501478 1103 92-93 1.140730267944761 1103 94-95 1.5598143661385855 1103 96-97 2.492264345322905 1103 98-99 3.566036062795366 1103 100-101 3.5912482594455213 1104 1 0.41184142097055343 1104 2 0.8834336600822539 1104 3 1.127427089982909 1104 4 1.408784944596178 1104 5 -0.18617202068662664 1104 6 0.3604516730157741 1104 7 0.3545305514427355 1104 8 0.40878703662091453 1104 9 0.8396472542304494 1104 10-11 1.057245745611091 1104 12-13 1.4218804226839907 1104 14-15 2.115980507116028 1104 16-17 1.5193027312917238 1104 18-19 1.8344357506753894 1104 20-21 1.3823013970325277 1104 22-23 1.931057928637692 1104 24-25 2.3578267842471163 1104 26-27 2.953758462838259 1104 28-29 2.7489185303922596 1104 30-31 2.6110717768098155 1104 32-33 2.0839715237835534 1104 34-35 1.5227941309400634 1104 36-37 1.9496692714727502 1104 38-39 1.7843592429577555 1104 40-41 1.8967397333963305 1104 42-43 2.835960085223398 1104 44-45 2.3718255543673052 1104 46-47 3.088074166112232 1104 48-49 1.4820034078114226 1104 50-51 -0.09097156365942283 1104 52-53 -0.16399325016088895 1104 54-55 0.23900318664970754 1104 56-57 1.0083379939366743 1104 58-59 1.5653790337825484 1104 60-61 0.8212089638665248 1104 62-63 0.25832468390112595 1104 64-65 0.712951444135907 1104 66-67 0.6983366791707546 1104 68-69 0.7242206825215405 1104 70-71 1.7579298319503849 1104 72-73 2.896356540476013 1104 74-75 1.9501897358739235 1104 76-77 2.1325335683976157 1104 78-79 2.2027608482570074 1104 80-81 2.3722854435121334 1104 82-83 2.6584245892294085 1104 84-85 1.8802545397440227 1104 86-87 1.2409659583614499 1104 88-89 2.3150149132402795 1104 90-91 3.082598891098499 1104 92-93 2.615455542670034 1104 94-95 3.408532314856533 1104 96-97 4.047575700634262 1104 98-99 3.5235452203045234 1104 100-101 2.1165229847202482 1105 1 -0.7310157218865889 1105 2 -0.6879949113463191 1105 3 -1.0725729100170902 1105 4 -1.2197864839752484 1105 5 -0.32902916354377254 1105 6 -0.3538340412699341 1105 7 0.6402448371570202 1105 8 -0.44835582052193956 1105 9 0.21107582565902305 1105 10-11 0.07153145989680354 1105 12-13 0.3218804226839964 1105 14-15 0.3731233642588876 1105 16-17 0.8907313027202903 1105 18-19 0.620150036389667 1105 20-21 0.38230139703252064 1105 22-23 0.5310579286376935 1105 24-25 1.2721124985328274 1105 26-27 0.9394727485525465 1105 28-29 1.1203471018208333 1105 30-31 1.596786062524103 1105 32-33 -0.31602847621644514 1105 34-35 0.008508416654358086 1105 36-37 -0.007473585670105365 1105 38-39 0.6700735286720416 1105 40-41 -0.7461174094608118 1105 42-43 0.47881722808053695 1105 44-45 -0.042460159918409346 1105 46-47 0.273788451826519 1105 48-49 0.9105748363828532 1105 50-51 1.2518855791977224 1105 52-53 1.6645781784105402 1105 54-55 0.5532889009354207 1105 56-57 0.8940522796509605 1105 58-59 1.2510933194968317 1105 60-61 0.8069232495808087 1105 62-63 0.9868961124725573 1105 64-65 1.312951444135905 1105 66-67 1.869765250599329 1105 68-69 1.695649253950112 1105 70-71 2.1150726890932425 1105 72-73 2.296356540476012 1105 74-75 2.3073325930167776 1105 76-77 2.1325335683976157 1105 78-79 3.102760848257006 1105 80-81 2.972285443512135 1105 82-83 2.615567446372264 1105 84-85 2.2659688254583052 1105 86-87 2.8695373869328797 1105 88-89 3.5150149132402753 1105 90-91 2.711170319669929 1105 92-93 2.201169828384323 1105 94-95 2.1228180291422483 1105 96-97 4.661861414919976 1105 98-99 5.166402363161666 1105 100-101 5.545094413291675 1106 1 -0.4302220710929383 1106 2 -0.02847110182250745 1106 3 0.632188994744812 1106 4 0.5548166906279235 1106 5 0.16938353486892765 1106 6 0.9445786571427632 1106 7 1.5227845196967067 1106 8 0.5762473540812323 1106 9 -0.32146385688066204 1106 10-11 0.2139917773571156 1106 12-13 -0.010659259855692937 1106 14-15 -0.45306711193158833 1106 16-17 0.2744614614504499 1106 18-19 -0.1350086937690591 1106 20-21 -0.016904952173824483 1106 22-23 0.8540738016535627 1106 24-25 -0.3632049617846249 1106 26-27 1.1815362406160403 1106 28-29 0.7516963081700396 1106 30-31 -0.9678964771584404 1106 32-33 1.2863524761645024 1106 34-35 0.8243814325273675 1106 36-37 -0.7523148555113792 1106 38-39 -0.5172280586295486 1106 40-41 -0.6746888380322389 1106 42-43 -0.43030975604644794 1106 44-45 0.04166682420857626 1106 46-47 -0.7563702783322093 1106 48-49 0.20382880463682085 1106 50-51 0.4050601823723241 1106 52-53 0.23441944825180983 1106 54-55 0.6774952501417708 1106 56-57 1.8150840256827045 1106 58-59 2.187998081401595 1106 60-61 2.2311295987871596 1106 62-63 2.3194357950122395 1106 64-65 3.3696974758819387 1106 66-67 3.024527155361234 1106 68-69 1.8174746507755088 1106 70-71 1.2424536414741922 1106 72-73 0.28008669920616924 1106 74-75 0.662094497778682 1106 76-77 0.16983515569920016 1106 78-79 0.6765703720665321 1106 80-81 0.8853806816073693 1106 82-83 1.3889801447849628 1106 84-85 1.921127555617037 1106 86-87 1.9885850059804966 1106 88-89 1.3677926910180567 1106 90-91 2.2429163514159605 1106 92-93 2.6813285585430506 1106 94-95 2.605754537078756 1106 96-97 -0.06115445809589559 1106 98-99 0.8406087123680166 1106 100-101 0.592316635513896 1107 1 1.3080820224743128 1107 2 0.5285464420371397 1107 3 1.1672767140430587 1107 4 0.9027699070021953 1107 5 1.1401437687870555 1107 6 1.467970470008261 1107 7 0.9906207770066473 1107 8 0.9636742546660315 1107 9 0.46069988580939736 1107 10-11 0.46326078320507236 1107 12-13 1.1399255354659488 1107 14-15 1.84079253719122 1107 16-17 1.7181749117428495 1107 18-19 0.9344357506753838 1107 20-21 1.1424517729723789 1107 22-23 0.580681988788065 1107 24-25 0.5585786639463635 1107 26-27 -0.20150469505647806 1107 28-29 -0.3616077853972115 1107 30-31 -0.11336431341575093 1107 32-33 -0.39347208523900434 1107 34-35 0.18256856703029456 1107 36-37 1.0335038579389177 1107 38-39 -0.15173098260616058 1107 40-41 0.6937322145993363 1107 42-43 0.9607721152985818 1107 44-45 1.5519007423372244 1107 46-47 1.0564952187438124 1107 48-49 -1.22965072752692 1107 50-51 -0.9969866012534077 1107 52-53 -1.939557159935326 1107 54-55 -1.7252825276360078 1107 56-57 -2.8698574947851263 1107 58-59 -3.9940194624580556 1107 60-61 -3.9874376526748314 1107 62-63 -4.581149000309399 1107 64-65 -5.131033518270108 1107 66-67 -4.243016704287889 1107 68-69 -3.6291627761250744 1107 70-71 -3.917634077824051 1107 72-73 -3.6972524820803763 1107 74-75 -3.3817651513441227 1107 76-77 -4.485135604534715 1107 78-79 -4.003254189336978 1107 80-81 -3.3010228271645587 1107 82-83 -2.2915754107705943 1107 84-85 -1.4377905730379332 1107 86-87 -3.2394851694580993 1107 88-89 -2.811300876233407 1107 90-91 -3.461762011157141 1107 92-93 -2.8815369385329745 1107 94-95 -2.530941369353993 1107 96-97 -2.879867908388295 1107 98-99 -2.220063802251868 1107 100-101 -1.4522740077609573 1108 1 0.0712399172111553 1108 2 0.08117802098450966 1108 3 0.6935925035167436 1108 4 1.1132962227916678 1108 5 0.6927753477344254 1108 6 0.8627073121135211 1108 7 1.122199724375065 1108 8 1.0163058336134014 1108 9 0.27648935949360975 1108 10-11 0.5422081516261272 1108 12-13 0.6136097459922638 1108 14-15 0.8276346424543704 1108 16-17 0.8760696485849522 1108 18-19 0.6975936454122262 1108 20-21 -0.06807454281710079 1108 22-23 -0.3666864322645651 1108 24-25 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0.5206020489556238 2312 7 0.5432523559540101 2312 8 0.3320953072976067 2312 9 0.22385778054623984 2312 10-11 0.01589236215243517 2312 12-13 -0.5179692013761539 2312 14-15 0.6828978003491102 2312 16-17 0.3760696485849522 2312 18-19 -0.2629326703772463 2312 20-21 -0.8443903322907858 2312 22-23 -0.669318011211935 2312 24-25 -0.07300028342205422 2312 26-27 0.5616531996803715 2312 28-29 0.2699711619712062 2312 30-31 0.4787409497421464 2312 32-33 0.04073844107678326 2312 34-35 -0.3437472224433904 2312 36-37 -0.28228561574529465 2312 38-39 -0.1912046668166809 2312 40-41 0.23320589880987086 2312 42-43 -0.3023857794382607 2312 44-45 -1.9744150471364534 2312 46-47 -1.022452149677239 2312 48-49 -1.0322823064742899 2312 50-51 -0.9706708117797227 2312 52-53 -0.9921887388826924 2312 54-55 -0.17265094868863784 2312 56-57 -1.001436442153551 2312 58-59 -1.1650720940370043 2312 60-61 -1.145332389516934 2312 62-63 -0.4364121582041349 2312 64-65 -0.1441914130069506 2312 66-67 -0.46670091481420783 2312 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48-49 0.23601037645253697 2314 50-51 -1.5996823650531518 2314 52-53 -1.4918678146208215 2314 54-55 -1.3777215520262516 2314 56-57 -1.570756082718379 2314 58-59 -1.4105791543707653 2314 60-61 -0.9094530570393999 2314 62-63 -2.1270411697574083 2314 64-65 -3.4612645837386573 2314 66-67 -2.4368549584599073 2314 68-69 -2.089368167652676 2314 70-71 -1.652871561777836 2314 72-73 -1.7527723793846128 2314 74-75 -0.9700193233595265 2314 76-77 -0.2685117277696314 2314 78-79 -1.0205840994781852 2314 80-81 -2.668481107010514 2314 82-83 -2.773282727843764 2314 84-85 -1.6361217668761867 2314 86-87 -1.8203580834503974 2314 88-89 -2.163033867247524 2314 90-91 -2.109387171619268 2314 92-93 -2.5740914956574876 2314 94-95 -2.040248172948342 2314 96-97 -0.622807574627064 2314 98-99 -1.562169065409762 2314 100-101 -0.7914909525619844 2315 1 -1.8536638055102834 2315 2 -0.262210939220882 2315 3 -1.8474858020031526 2315 4 -2.163340490943888 2315 5 -0.9959281182476047 2315 6 -0.29390372768108364 2315 7 -0.3932046401948952 2315 8 0.43735846519234656 2315 9 0.015257010328014076 2315 10-11 -0.6779458920195722 2315 12-13 -0.8202798560616529 2315 14-15 -1.5038801200616803 2315 16-17 -1.38034883665253 2315 18-19 -1.0655642493246162 2315 20-21 -1.696444247566781 2315 22-23 -1.4745169842542936 2315 24-25 -2.428584365578665 2315 26-27 -1.9438025127714909 2315 28-29 -1.9803497622906647 2315 30-31 -2.08544390263269 2315 32-33 -1.2644605319655824 2315 34-35 -1.4859166704536548 2315 36-37 0.23921630980027686 2315 38-39 0.3418505321563572 2315 40-41 0.06921360099214979 2315 42-43 -1.127802980978693 2315 44-45 -0.08705946305429535 2315 46-47 1.3222205075756541 2315 48-49 1.2482054984037596 2315 50-51 -1.002121389443392 2315 52-53 -1.7479653755964293 2315 54-55 -1.1338191130018593 2315 56-57 -0.9975853510110575 2315 58-59 -1.1178962275414968 2315 60-61 -2.1045750082589123 2315 62-63 -2.3831387307330196 2315 64-65 -2.924679217885 2315 66-67 -2.08319642187454 2315 68-69 -1.8210754847258457 2315 70-71 -2.7626276593388113 2315 72-73 -2.3259431110919344 2315 74-75 -1.3724583477497738 2315 76-77 -1.9758288009403664 2315 78-79 -1.9839987336245244 2315 80-81 -1.4123835460349063 2315 82-83 -0.7976729717462057 2315 84-85 -0.07514615712009132 2315 86-87 -0.17401662003576135 2315 88-89 -0.08986313554020953 2315 90-91 -0.0728018057656108 2315 92-93 -0.3057988127306608 2315 94-95 1.0573128026614107 2315 96-97 2.2552412058607416 2315 98-99 1.498806544346337 2315 100-101 3.098752949877042 2316 1 -2.560980878681015 2316 2 -2.189040207513564 2316 3 -3.4328516556616897 2316 4 -4.553584393382913 2316 5 -2.9715378743451666 2316 6 -2.5378061667054794 2316 7 -1.2956436645851426 2316 8 -2.367519583588141 2316 9 -1.4969381116232086 2316 10-11 -2.446238574946406 2316 12-13 -3.198328636549455 2316 14-15 -4.174611827378747 2316 16-17 -3.3925439586037456 2316 18-19 -2.8582471761538883 2316 20-21 -3.1720540036643428 2316 22-23 -4.401346252546979 2316 24-25 -6.050535585090863 2316 26-27 -5.968192756673936 2316 28-29 -5.224252201315057 2316 30-31 -5.817151219705863 2316 32-33 -3.2888507758680134 2316 34-35 -3.485916670453662 2316 36-37 -3.3461495438582602 2316 38-39 -3.658149467843643 2316 40-41 -3.028347374617603 2316 42-43 -2.835120054149428 2316 44-45 -2.2821814142738077 2316 46-47 -2.8119258338877664 2316 48-49 -2.288379867449901 2316 50-51 -2.5265116333458337 2316 52-53 -1.7357702536452102 2316 54-55 -2.414306917879909 2316 56-57 -3.9731951071086193 2316 58-59 -4.191066959248815 2316 60-61 -3.6899408619174494 2316 62-63 -2.883138730733016 2316 64-65 -3.753947510567926 2316 66-67 -3.3027086169964903 2316 68-69 -3.101563289603895 2316 70-71 -5.091895952021741 2316 72-73 -5.240577257433397 2316 74-75 -4.7017266404327 2316 76-77 -4.32948733752573 2316 78-79 -4.41082800191721 2316 80-81 -3.802627448473931 2316 82-83 -4.212307118087669 2316 84-85 -3.5019754254127697 2316 86-87 -3.8081629614991783 2316 88-89 -7.0776680135889904 2316 90-91 -6.63377741552171 2316 92-93 -7.171652471267247 2316 94-95 -6.85732134368005 2316 96-97 -5.976466111212428 2316 98-99 -6.452412967848787 2316 100-101 -6.352466562318082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 575.0 3 149.0 4 145.0 5 110.0 6 115.0 7 104.0 8 142.0 9 184.0 10 200.0 11 231.0 12 318.0 13 484.0 14 521.0 15 783.0 16 964.0 17 1151.0 18 929.0 19 719.0 20 706.0 21 786.0 22 711.0 23 780.0 24 800.0 25 827.0 26 864.0 27 926.0 28 1000.0 29 1044.0 30 1321.0 31 1442.0 32 1530.0 33 1946.0 34 2555.0 35 4232.0 36 3490.0 37 3129.0 38 2038.0 39 283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.31430365505874 22.45362497056592 18.175871902880615 12.056199471494727 2 15.930269081771542 26.84012145325097 36.88357240079573 20.34603706418176 3 21.524651941798385 32.39558253951638 33.91081335706061 12.16895216162462 4 10.451474362331744 24.1266432724035 37.99821924265437 27.42366312261038 5 8.650120431458792 41.28966383914546 34.443397214368 15.616818515027752 6 20.662931350473894 39.87537309525056 17.890244541027386 21.571451013248154 7 16.742152525067414 43.36989815954133 23.47304762154096 16.414901693850304 8 22.844127964814913 30.873344154144196 21.265511283313263 25.017016597727633 9 22.7450056293038 22.598381902442856 25.481109103762467 29.175503364490872 10-11 22.313714195947007 30.254752055296642 24.11504424778761 23.316489500968736 12-13 27.8585734295027 31.574882515413716 26.404251698454047 14.162292356629532 14-15 22.607545885371668 32.26245647108109 31.768910533343806 13.36108711020344 16-17 13.862449102501998 36.695950457586505 31.657916443001348 17.783683996910145 18-19 13.274440518256773 34.691794267766 36.916633948436065 15.11713126554116 20-21 16.245780231858266 31.448721639232723 36.146075942742 16.15942218616701 22-23 14.77497284493476 31.731511653776188 38.09037729182207 15.403138209466977 24-25 16.63155966663614 32.68876009053681 36.65694137349051 14.022738869336543 26-27 14.947866926568897 31.70763615431913 40.15620298538704 13.188293933724932 28-29 12.818163973041944 36.01125433488189 37.59602172348361 13.574559968592553 30-31 13.341531698893222 34.21073288155106 37.00122975483398 15.446505664721736 32-33 13.448456305599162 34.58791208791209 38.28231292517007 13.681318681318682 34-35 14.83196630168884 34.09991758565206 37.01189121306071 14.056224899598394 36-37 13.501314808273479 33.795151562724854 39.2100684223609 13.493465206640762 38-39 13.799960750964871 34.9878982141689 37.44881271668738 13.763328318178845 40-41 13.103836290364788 35.8598942795834 36.96681844350238 14.06945098654943 42-43 12.840003663339134 36.08258213075504 34.762471707246874 16.314942498658958 44-45 13.836815321620596 34.10473437029866 35.57253306471658 16.485917243364163 46-47 15.86761724115377 35.45555628229446 35.55889855451632 13.117927922035449 48-49 14.83027012885081 34.30047746746027 35.50657335339133 15.3626790502976 50-51 12.332565927166177 35.399225617413144 36.93098576810381 15.337222687316867 52-53 14.29711415190602 36.32819654116847 32.70453415661547 16.67015515031004 54-55 13.558523790211801 33.506456128416126 34.62368686141891 18.311333219953166 56-57 15.721575297657989 32.782938636660994 33.083867591259974 18.411618474421036 58-59 16.755200837367525 32.45322517336125 34.091325395786996 16.700248593484236 60-61 15.087723888896157 33.278819358130654 36.55750788272081 15.075948870252379 62-63 13.55184821720641 33.600261694471705 35.877003598298984 16.9708864900229 64-65 13.283099826014155 35.28773072747014 34.28044425258035 17.148725193935352 66-67 14.390767298230923 32.81168219407516 32.989636763320426 19.807913744373494 68-69 13.805916447514752 32.104774240818514 35.71587444884929 18.373434862817444 70-71 16.576190040158544 31.8966081075778 33.3237406307638 18.20346122149986 72-73 15.344372073556725 30.093646184833506 36.782285699338196 17.779696042271574 74-75 14.086312677419777 33.419672174038176 34.566932224010046 17.927082924532005 76-77 14.574639264269173 30.25601444251122 34.73790243455737 20.431443858662234 78-79 15.682862040538595 29.7536017586789 35.560906033681846 19.002630167100666 80-81 16.54806132593794 29.86238292083094 33.57621265239914 20.01334310083198 82-83 15.058491010442019 29.236345554944908 36.28797990107561 19.417183533537465 84-85 15.463836580865514 30.04959629401835 34.115445516050094 20.371121609066044 86-87 15.27379784102061 30.947988223748773 33.90906117108276 19.869152764147856 88-89 15.664637738071862 29.871064860265722 32.19713332024347 22.26716408141894 90-91 16.04532785490899 28.209523560277933 33.493411496839876 22.2517370879732 92-93 17.712027428451783 29.22648101862151 33.87727861601476 19.184212936911944 94-95 17.303338437176922 27.80678680329263 34.030858623532644 20.859016135997802 96-97 15.307898697729206 28.39473856423009 35.30658988286107 20.990772855179635 98-99 17.802013642493357 28.6531638277537 31.863470325613065 21.68135220413988 100-101 17.42818988766987 27.170013877615144 33.235579063130054 22.16621717158493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1823.0 1 1157.5 2 472.0 3 390.5 4 302.5 5 301.5 6 445.5 7 820.0 8 1101.0 9 1033.5 10 699.0 11 420.0 12 350.0 13 308.0 14 287.0 15 251.5 16 227.5 17 229.0 18 220.0 19 209.5 20 225.0 21 236.5 22 243.0 23 279.0 24 298.0 25 322.0 26 376.5 27 394.5 28 419.0 29 481.5 30 518.0 31 551.5 32 617.0 33 661.0 34 658.0 35 669.5 36 702.0 37 695.0 38 653.0 39 605.5 40 551.0 41 512.0 42 489.5 43 462.5 44 458.0 45 522.0 46 747.0 47 1548.0 48 3224.0 49 3205.5 50 1615.0 51 833.5 52 502.5 53 393.0 54 333.0 55 275.5 56 262.0 57 255.5 58 290.5 59 338.5 60 370.5 61 351.5 62 268.0 63 182.0 64 102.0 65 72.5 66 57.5 67 54.0 68 42.0 69 29.5 70 23.5 71 15.0 72 17.0 73 18.0 74 14.5 75 13.5 76 9.5 77 6.5 78 7.5 79 8.5 80 4.0 81 0.5 82 0.5 83 0.5 84 1.0 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0340011508081812 2 0.07846419417272585 3 0.05754040905999895 4 0.12554271067636136 5 0.09938797928545273 6 0.10461892556363446 7 0.09677250614636188 8 0.09415703300727102 9 0.10723439870272533 10-11 0.10461892556363446 12-13 0.09808024271590732 14-15 0.10723439870272533 16-17 0.11638855468954334 18-19 0.07584872103363499 20-21 0.054924935920908094 22-23 0.07192551132499869 24-25 0.04577077993409008 26-27 0.037924360516817496 28-29 0.06931003818590784 30-31 0.039232097086362924 32-33 0.03661662394727206 34-35 0.03269341423863577 36-37 0.04053983365590836 38-39 0.04315530679499922 40-41 0.05230946278181723 42-43 0.04577077993409008 44-45 0.03530888737772663 46-47 0.030077941099544907 48-49 0.030077941099544907 50-51 0.026154731390908615 52-53 0.0340011508081812 54-55 0.037924360516817496 56-57 0.049693989642726366 58-59 0.049693989642726366 60-61 0.04577077993409008 62-63 0.05623267249045352 64-65 0.03269341423863577 66-67 0.05754040905999895 68-69 0.04838625307318094 70-71 0.027462467960454047 72-73 0.013077365695454308 74-75 0.03269341423863577 76-77 0.03530888737772663 78-79 0.06146361876863524 80-81 0.03138567766909034 82-83 0.060155882199089815 84-85 0.06669456504681698 86-87 0.05623267249045352 88-89 0.09546476957681645 90-91 0.06146361876863524 92-93 0.06669456504681698 94-95 0.07192551132499869 96-97 0.08238740388136215 98-99 0.11638855468954334 100-101 0.11246534498090704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 38234.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.11691164931736 #Duplication Level Percentage of deduplicated Percentage of total 1 87.07246376811594 39.28440654914474 2 7.101449275362319 6.407909190772611 3 2.318840579710145 3.138567766909034 4 1.0492753623188407 1.8936025527017837 5 0.49275362318840576 1.1115760841136162 6 0.3710144927536232 1.0043416854108909 7 0.2144927536231884 0.6774075430245332 8 0.1681159420289855 0.6067897682690799 9 0.11594202898550725 0.4707851650363551 >10 0.8231884057971015 6.870847936391693 >50 0.08115942028985507 2.644243343620861 >100 0.1507246376811594 14.073860961447926 >500 0.02318840579710145 8.181199979076215 >1k 0.017391304347826087 13.634461474080661 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2237 5.850813412146257 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1841 4.815086049066276 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 2.968562012868128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 951 2.4873149552754095 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 805 2.1054558769681435 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 748 1.9563739080399645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 1.6320552387926976 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 462 1.2083485902599782 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 350 0.9154155986818016 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 284 0.7427943715018047 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 273 0.7140241669718053 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 266 0.6957158549981692 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 265 0.6931003818590783 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 257 0.6721765967463514 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 248 0.6486373384945338 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 243 0.6355599727990794 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 224 0.585865983156353 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 224 0.585865983156353 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.5466338860699901 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202 0.5283255740963541 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 187 0.4890934770099911 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 184 0.4812470575927185 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 172 0.4498613799236282 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 169 0.4420149605063556 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 159 0.41586022911544696 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 153 0.4001673902809018 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.37139718575090236 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 140 0.36616623947272064 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.3164722498299943 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 121 0.3164722498299943 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 119 0.31124130355181256 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.2720092064654496 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.26939373332635874 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.24323900193545012 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 86 0.2249306899618141 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 86 0.2249306899618141 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.21708427054454152 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 77 0.20139143170999635 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 76 0.19877595857090546 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 71 0.18569859287545118 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 70 0.18308311973636032 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 70 0.18308311973636032 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.1778521734581786 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 62 0.16215933462363344 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.15954386148454255 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 55 0.14385102264999738 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 53 0.13862007637181567 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 48 0.12554271067636136 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.11769629125908877 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 45 0.11769629125908877 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.10723439870272533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 40 0.10461892556363446 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.10461892556363446 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0026154731390908613 0.0 12-13 0.0 0.0 0.0 0.005230946278181723 0.0 14-15 0.0 0.0 0.0 0.005230946278181723 0.0 16-17 0.0 0.0 0.0 0.006538682847727154 0.0 18-19 0.0 0.0 0.0 0.007846419417272585 0.0 20-21 0.0 0.0 0.0 0.01569283883454517 0.0 22-23 0.0 0.0 0.0 0.03530888737772663 0.0 24-25 0.0 0.0 0.0 0.05230946278181723 0.0 26-27 0.0 0.0 0.0 0.08238740388136213 0.0 28-29 0.0 0.0 0.0 0.15954386148454255 0.0 30-31 0.0 0.0 0.0 0.3177799863995397 0.0 32-33 0.0 0.0 0.0 0.5126327352618089 0.0 34-35 0.0 0.0 0.0 0.7179473766804415 0.0 36-37 0.0 0.0 0.0 0.9101846524036199 0.0 38-39 0.0 0.0 0.0 1.0644975676099806 0.0 40-41 0.0 0.0 0.0 1.2959669404195218 0.0 42-43 0.0 0.0 0.0 1.5692838834545169 0.0 44-45 0.0 0.0 0.0 1.809907412250876 0.0 46-47 0.0 0.0 0.0 2.041376785060417 0.0 48-49 0.0 0.0 0.0 2.2205366950881418 0.0 50-51 0.0 0.0 0.0 2.4585447507454097 0.0 52-53 0.0 0.0 0.0 2.9005597112517654 0.0 54-55 0.0 0.0 0.0 3.53742742062039 0.0 56-57 0.0 0.0 0.0 3.8225139927812943 0.0 58-59 0.0 0.0 0.0 4.154679081445833 0.0 60-61 0.0 0.0 0.0 4.529999476905372 0.0 62-63 0.0 0.0 0.0 4.697389757807187 0.0 64-65 0.0 0.0 0.0 4.862164565569912 0.0 66-67 0.0 0.0 0.0 5.306794999215358 0.0 68-69 0.0 0.0 0.0 5.614113093058535 0.0 70-71 0.0 0.0 0.0 5.87566040696762 0.0 72-73 0.0 0.0 0.0 6.2797510069571585 0.0 74-75 0.0 0.0 0.0 6.696918972642151 0.0 76-77 0.0 0.0 0.0 7.115394674896689 0.0 78-79 0.0 0.0 0.0 7.441021080713501 0.0 80-81 0.0 0.0 0.0 7.9340377674321285 0.0 82-83 0.0 0.0 0.0 8.338128367421668 0.0 84-85 0.0 0.0 0.0 8.68075534864257 0.0 86-87 0.0 0.0 0.0 9.162002406235288 0.0 88-89 0.0 0.0 0.0 9.734791023696186 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 95 0.0 54.999996 9 CAGAGAA 75 9.258656E-10 31.666668 10-11 GCAGAGA 75 3.2514436E-8 28.500002 10-11 CAGAAAA 115 3.6379788E-12 26.847824 10-11 GAGTACT 555 0.0 23.108109 12-13 GCAGAAA 115 3.4942786E-9 22.71739 10-11 GTACTTT 610 0.0 21.02459 14-15 ACTTTTT 675 0.0 19.703703 16-17 AGTACAT 290 2.228262E-9 19.655172 7 AGTACTT 530 0.0 19.268867 12-13 GAGAAAA 115 2.007113E-6 18.586956 12-13 TGTAGAT 65 0.0075002457 18.269232 94-95 AGAGAAA 110 2.8598899E-5 17.272728 12-13 GAGTACA 360 1.718945E-9 17.152777 6 TACATGG 335 1.3331373E-8 17.014925 2 GTGTAGA 85 0.0017670126 16.764706 94-95 GTACATG 340 1.6007107E-8 16.764706 1 GTGTGTA 85 0.0017670126 16.764706 92-93 GACGATA 195 5.2477844E-9 15.833334 94-95 CAGTGTG 105 3.9422986E-4 15.833332 90-91 >>END_MODULE