FastQCFastQC Report
Thu 26 May 2016
SRR937000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36882
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23046.246949731576379No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21585.851092673933084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10392.817092348571119No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10012.714061059595467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8952.426657990347595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6321.7135730166476872No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4821.30687056016485No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4281.1604576758310285No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3040.8242503118052167No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3010.8161162626755598No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2910.789002765576704No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2750.7456211702185348No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2740.7429098205086493No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2560.6941055257307088No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.6615693292120818No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2350.6371671818231115No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.5937855864649422No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.569383439075972No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2060.5585380402364297No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2010.5449812916870017No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1960.5314245431375738No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1700.4609294506805488No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1670.45279540155089204No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC1430.38772300851363806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.37416625996421016No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1350.36603221083455345No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1340.3633208611246678No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.31451656634672737No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.28740306924787157No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1050.284691719537986No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1020.27655767040832924No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT980.2657122715687869No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG970.26300092185890134No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC900.2440214738897023No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA890.2413101241798167No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA890.2413101241798167No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.21961932650073207No Hit
GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.1979285288216474No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA720.19521717911176184No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG650.17623773114256278No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.16268098259313485No Hit
TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.15725828317336368No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.14912423404370698No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.14912423404370698No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.14641288433382138No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG500.13556748549427905No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG490.13285613578439345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG450.12201073694485115No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT440.11929938723496557No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG430.11658803752507998No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT420.1138766878151944No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT410.11116533810530882No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA410.11116533810530882No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.11116533810530882No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT400.10845398839542324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC390.10574263868553767No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA390.10574263868553767No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA380.10303128897565209No Hit
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC380.10303128897565209No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.1003199392657665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA1001.2545534E-633.259
CGGGTCG300.003927213631.66666814-15
CAGAGAA701.7503513E-523.7510-11
AGTACTT5600.020.7812512-13
GAGTACT5750.019.82608612-13
GAGAAAA857.825783E-519.55882512-13
GTACTTT5850.019.48717914-15
CAGAAAA1101.3101981E-619.43181810-11
TACTTTT6150.018.92276414-15
ACTTTTT6350.018.3267716-17
CATGGGC800.00117671617.812516-17
GCAGAAA1300.00198304214.6153858
AGAGTAC10700.014.20560710-11
CTTGAAA1050.007207214413.57142856-57
GTACATG4252.466386E-713.4117651
TACATGG4252.466386E-713.4117652
CTTTTTT8900.013.07584316-17
GCTTGAA1100.00980117912.95454656-57
ACATGGG4504.942958E-712.6666663
GTATCAA22200.010.6981981
Produced by FastQC (version 0.11.5)