FastQCFastQC Report
Thu 26 May 2016
SRR936999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244116
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73130.32587442003889283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54870.24450607722595447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.15226485618390495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53450.040.3688961
GTATCAA88350.035.8266681
ATCAACG105550.029.5852323
TCAACGC105800.029.4254744
CAACGCA107350.028.9980165
TATCAAC108600.028.928112
AACGCAG109400.028.6718466
ACGCAGA124650.024.6678527
CGCAGAG125500.024.4255898
GAGTACT65500.022.85258312-13
TACATGG78350.022.8085292
GTACATG82000.022.7780971
GCAGAGT134050.022.0522359
GTATAGG6800.020.9677141
ACATGGG81850.020.6727943
GTACTTT72500.020.4822714-15
AGAGTAC109250.020.09515810-11
AGTACTT70600.019.58638812-13
CAGAGTA131600.018.68638810-11
ACTTTTT85450.017.43397316-17
Produced by FastQC (version 0.11.5)