FastQCFastQC Report
Thu 26 May 2016
SRR936999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244116
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76510.3409360300447927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.27578788262282344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41030.18283368595919283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62950.037.357031
GTATCAA91600.031.5334151
ATCAACG112350.025.4495353
TCAACGC113800.025.1252634
TATCAAC115200.024.9853972
CAACGCA115500.024.7554575
AACGCAG118650.024.0987686
GAGTACT73800.021.8818412-13
TACATGG75000.021.59532
GTACATG76750.021.5408951
ACGCAGA133650.021.2163897
CGCAGAG134050.020.9404878
GTACTTT76550.020.78621314-15
CGTATAC1404.8565227E-420.35543
ACATGGG77200.020.3026663
AGAGTAC114950.019.37893910-11
ACCGTAC1507.7274814E-418.9987968
AGTACTT76850.018.85025412-13
GCAGAGT147100.018.5984139
GACCGTA1851.5228921E-417.9718347
Produced by FastQC (version 0.11.5)