##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936999_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244116 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1014172172918 31.0 31.0 34.0 31.0 34.0 2 32.09392829960662 33.0 31.0 34.0 30.0 34.0 3 32.28259635419916 33.0 31.0 34.0 30.0 34.0 4 36.02533647993241 37.0 35.0 37.0 35.0 37.0 5 35.9471190437571 37.0 35.0 37.0 35.0 37.0 6 35.98467681706293 37.0 35.0 37.0 35.0 37.0 7 35.92655014268425 37.0 35.0 37.0 35.0 37.0 8 35.895931404615446 37.0 35.0 37.0 35.0 37.0 9 37.582007792823546 39.0 37.0 39.0 35.0 39.0 10-11 37.50254198089581 39.0 37.0 39.0 35.0 39.0 12-13 37.41020785021808 39.0 37.0 39.0 34.0 39.0 14-15 38.61919615563545 40.0 38.0 41.0 34.0 41.0 16-17 38.5218961497534 40.0 38.0 41.0 34.0 41.0 18-19 38.56257408262318 40.0 38.0 41.0 34.0 41.0 20-21 38.63901866035445 40.0 38.0 41.0 34.0 41.0 22-23 38.56985801981716 40.0 38.0 41.0 34.0 41.0 24-25 38.471344395744254 40.0 38.0 41.0 34.0 41.0 26-27 38.31849757320923 40.0 38.0 41.0 34.0 41.0 28-29 38.14698683134027 40.0 38.0 41.0 33.5 41.0 30-31 38.03521564838894 40.0 37.0 41.0 33.0 41.0 32-33 38.04938626167275 40.0 37.0 41.0 33.0 41.0 34-35 37.97823842439517 40.0 37.0 41.0 33.0 41.0 36-37 37.838177259999036 40.0 37.0 41.0 33.0 41.0 38-39 37.68782473811514 40.0 37.0 41.0 33.0 41.0 40-41 37.51916055141535 40.0 37.0 41.0 32.5 41.0 42-43 37.313496272028715 40.0 37.0 41.0 32.0 41.0 44-45 37.013619616811255 39.5 36.0 41.0 31.0 41.0 46-47 36.78907195528217 39.0 36.0 41.0 31.0 41.0 48-49 36.4901350910559 39.0 36.0 41.0 30.0 41.0 50-51 36.23576989781277 39.0 35.0 40.0 30.0 41.0 52-53 35.976752315833934 39.0 35.0 40.0 29.0 41.0 54-55 35.73541541524591 38.5 35.0 40.0 28.5 41.0 56-57 35.452851144949726 38.0 34.5 40.0 28.0 41.0 58-59 35.11493657190626 38.0 34.0 40.0 27.5 41.0 60-61 34.77091068376144 37.5 34.0 40.0 27.0 41.0 62-63 34.382876375374536 37.0 34.0 40.0 26.0 41.0 64-65 33.974618290676595 36.5 33.0 39.0 26.0 40.5 66-67 33.57671573127236 36.0 33.0 39.0 25.5 40.0 68-69 33.128102780783166 35.5 32.0 38.5 25.0 40.0 70-71 32.64261785041415 35.0 32.0 38.0 23.5 40.0 72-73 32.28522812546232 35.0 31.0 37.0 22.5 39.0 74-75 31.82892372765044 35.0 31.0 37.0 22.0 39.0 76-77 30.451056006017513 33.5 29.5 35.5 20.5 37.5 78-79 31.177181126109346 34.0 31.0 36.0 21.0 38.0 80-81 31.014570325241653 34.0 31.0 35.5 20.0 37.0 82-83 30.6828909468138 34.0 31.0 35.0 19.5 37.0 84-85 30.29727607663775 34.0 30.0 35.0 18.0 36.0 86-87 29.93168846886703 34.0 30.0 35.0 16.0 36.0 88-89 29.56207076639532 34.0 29.5 35.0 9.0 35.5 90-91 29.134419521985496 34.0 29.0 35.0 4.5 35.0 92-93 28.658115935183385 34.0 29.0 35.0 2.0 35.0 94-95 28.158582711410638 33.0 29.0 35.0 2.0 35.0 96-97 27.502954169927044 33.0 27.0 35.0 2.0 35.0 98-99 26.722386231371285 33.0 25.5 34.5 2.0 35.0 100-101 23.633538105873313 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3643075717466715 1101 2 0.2638396929840212 1101 3 0.2770677423391703 1101 4 0.24643584953179953 1101 5 0.27501142512483767 1101 6 0.2665636609761819 1101 7 0.30306255327140974 1101 8 0.3419164318539032 1101 9 0.42247793766294706 1101 10-11 0.40003184878391806 1101 12-13 0.4675195083772863 1101 14-15 0.6026710936243944 1101 16-17 0.5513000179793082 1101 18-19 0.5767682545347412 1101 20-21 0.522787343526133 1101 22-23 0.4242326161052361 1101 24-25 0.4761984259494483 1101 26-27 0.5136993319800212 1101 28-29 0.5564276366745133 1101 30-31 0.47411643478570653 1101 32-33 0.639736823759705 1101 34-35 0.6212568837741017 1101 36-37 0.5904634686960577 1101 38-39 0.5716204235087545 1101 40-41 0.556478855125718 1101 42-43 0.6013928858586723 1101 44-45 0.5524596502361376 1101 46-47 0.6756236740949291 1101 48-49 0.46387308856441933 1101 50-51 0.384595065438738 1101 52-53 0.309415909013147 1101 54-55 0.31050853472206796 1101 56-57 0.4272435262045846 1101 58-59 0.2883385421745359 1101 60-61 0.4514494390307817 1101 62-63 0.48659907345196274 1101 64-65 0.601178087197404 1101 66-67 0.35764634884878177 1101 68-69 0.2595851526709936 1101 70-71 0.38573447108851866 1101 72-73 0.4903523223575874 1101 74-75 0.49063689524972887 1101 76-77 0.26356933171182817 1101 78-79 0.502184160367257 1101 80-81 0.7133895959094154 1101 82-83 0.7541834916290604 1101 84-85 0.7072712132872958 1101 86-87 0.6566281571963266 1101 88-89 0.73330156674524 1101 90-91 0.9059311648538007 1101 92-93 0.9930828816153934 1101 94-95 1.1422733848965372 1101 96-97 1.148368576163854 1101 98-99 1.167811766492239 1101 100-101 1.2986386007124118 1102 1 0.29714433662974216 1102 2 0.16063326812297163 1102 3 0.19687521571436406 1102 4 0.16228690301938542 1102 5 0.19056895170648147 1102 6 0.15775526385454697 1102 7 0.25243873352545876 1102 8 0.2676685757826789 1102 9 0.2848419703322662 1102 10-11 0.2609679406195582 1102 12-13 0.2881402239448292 1102 14-15 0.42132185703832903 1102 16-17 0.49200814600958154 1102 18-19 0.4280395413349325 1102 20-21 0.3542688361292079 1102 22-23 0.29759405470237255 1102 24-25 0.3116861666968731 1102 26-27 0.38665013319211283 1102 28-29 0.3907308537218981 1102 30-31 0.2804662813221839 1102 32-33 0.41845422754049366 1102 34-35 0.4199757814642098 1102 36-37 0.5135263881777234 1102 38-39 0.49337851830143364 1102 40-41 0.5914429956718052 1102 42-43 0.4507252810569966 1102 44-45 0.6022374218414228 1102 46-47 0.6724489291198879 1102 48-49 0.6434991683174047 1102 50-51 0.5522259542284473 1102 52-53 0.6336346751416428 1102 54-55 0.5367440221249993 1102 56-57 0.6514571687496229 1102 58-59 0.6219049391242564 1102 60-61 0.588634418410706 1102 62-63 0.5531550794741449 1102 64-65 0.5012995517840508 1102 66-67 0.567297659904348 1102 68-69 0.26344754635663037 1102 70-71 0.4515695157237545 1102 72-73 0.48755659118469197 1102 74-75 0.32690649858678 1102 76-77 0.4999940876659785 1102 78-79 0.5194736270679066 1102 80-81 0.45800326457909435 1102 82-83 0.5558636516500215 1102 84-85 0.43873266198145444 1102 86-87 0.35147916226322806 1102 88-89 0.6859181789417725 1102 90-91 0.7486700899372529 1102 92-93 0.6680160967372117 1102 94-95 0.6821461248425855 1102 96-97 0.8354377400363227 1102 98-99 0.9442399794576453 1102 100-101 1.235457591077445 1103 1 0.09751626358329446 1103 2 -0.14062632449304147 1103 3 0.05350977268737722 1103 4 0.16474534018232845 1103 5 0.14969293975522646 1103 6 0.14267982467080031 1103 7 0.20832447756532702 1103 8 0.2506826718142179 1103 9 0.2073547088304224 1103 10-11 0.25062403216317364 1103 12-13 0.30977397507610505 1103 14-15 0.245115883891458 1103 16-17 0.32113250598528253 1103 18-19 0.32901956885752526 1103 20-21 0.27320673672553397 1103 22-23 0.38597939582137997 1103 24-25 0.3979050333114671 1103 26-27 0.3199555720039342 1103 28-29 0.38536208814745265 1103 30-31 0.3062352402986406 1103 32-33 0.4152761117224415 1103 34-35 0.46386874088697994 1103 36-37 0.5048121396561385 1103 38-39 0.5931249880947647 1103 40-41 0.4263948311313186 1103 42-43 0.48022590713403446 1103 44-45 0.5687367213789898 1103 46-47 0.4844844853366439 1103 48-49 0.43873602379898813 1103 50-51 0.34655778745639765 1103 52-53 0.494706940548717 1103 54-55 0.4656030742071735 1103 56-57 0.39166619169751726 1103 58-59 0.4479689601505328 1103 60-61 0.3833505761573264 1103 62-63 0.3405709310009044 1103 64-65 0.1951937509633339 1103 66-67 0.30315575737399314 1103 68-69 0.34246838666359736 1103 70-71 0.18151955353632587 1103 72-73 0.3675748156054155 1103 74-75 0.2692169088222087 1103 76-77 0.31652934014239165 1103 78-79 0.3195263167196565 1103 80-81 0.4659482441851033 1103 82-83 0.4211107971106536 1103 84-85 0.6049189229997936 1103 86-87 0.48964134721010666 1103 88-89 0.6957965588279649 1103 90-91 0.8511675794303137 1103 92-93 0.7695447165162861 1103 94-95 0.6441736226420183 1103 96-97 0.8506706282970669 1103 98-99 0.9260973826636558 1103 100-101 1.0531458171098542 1104 1 0.22148285171779492 1104 2 0.07910666615449458 1104 3 0.12170781652260132 1104 4 0.08802655671691895 1104 5 0.10544363583575489 1104 6 0.11126695967472955 1104 7 0.13493974136200393 1104 8 0.19201200714655187 1104 9 0.19979777301261947 1104 10-11 0.1760773769193662 1104 12-13 0.18170408145149253 1104 14-15 0.22537753877829658 1104 16-17 0.29467252169171587 1104 18-19 0.2464876205459703 1104 20-21 0.2048730464278279 1104 22-23 0.1491692294759801 1104 24-25 0.12574646973611436 1104 26-27 0.12467285621897162 1104 28-29 0.21447253292506474 1104 30-31 0.1788185914801943 1104 32-33 0.3423625684940532 1104 34-35 0.2590943423719523 1104 36-37 0.21148023418029993 1104 38-39 0.10833702493349051 1104 40-41 0.31469353892028096 1104 42-43 0.3296779668184797 1104 44-45 0.3780749094278306 1104 46-47 0.43459335947100897 1104 48-49 0.4057382369781166 1104 50-51 0.47209680366258766 1104 52-53 0.32671772087283557 1104 54-55 0.2559828843021208 1104 56-57 0.20851669501728765 1104 58-59 0.13301572248364835 1104 60-61 0.2301985807264657 1104 62-63 0.3336458221267691 1104 64-65 0.43409037408034834 1104 66-67 0.4802832650487332 1104 68-69 0.29558647047883824 1104 70-71 0.347061782421882 1104 72-73 0.36404622275898646 1104 74-75 0.34215186991308855 1104 76-77 0.3258657433693486 1104 78-79 0.5116284799237683 1104 80-81 0.6460581892125852 1104 82-83 0.6081851170935195 1104 84-85 0.7168807992045458 1104 86-87 0.67476485688384 1104 88-89 0.7672358391877836 1104 90-91 0.8395769461755158 1104 92-93 0.8336073280676537 1104 94-95 0.7393617428676187 1104 96-97 0.8854460977052199 1104 98-99 0.9471172298829025 1104 100-101 0.9064173956063186 1105 1 0.23634947307274956 1105 2 0.038211821490840236 1105 3 0.09237344690636462 1105 4 0.1034537564737974 1105 5 0.08315054847245307 1105 6 0.13472706312505522 1105 7 0.19836484117438857 1105 8 0.15907203694824545 1105 9 0.18922293032022708 1105 10-11 0.16679010204909162 1105 12-13 0.30598573342607693 1105 14-15 0.3065565980606664 1105 16-17 0.28645147515549496 1105 18-19 0.24779058887362027 1105 20-21 0.27443490546734495 1105 22-23 0.3016270474912872 1105 24-25 0.35776235826656944 1105 26-27 0.305601161047683 1105 28-29 0.3170914060050549 1105 30-31 0.3561507071468881 1105 32-33 0.4060833580129497 1105 34-35 0.4145681782819679 1105 36-37 0.2129723720511052 1105 38-39 0.25007720334662764 1105 40-41 0.06788572148677474 1105 42-43 0.13982130445710794 1105 44-45 0.4137164705325844 1105 46-47 0.23973084833986746 1105 48-49 0.37095225951487265 1105 50-51 0.4519785243576493 1105 52-53 0.47283369626214977 1105 54-55 0.5267824648608013 1105 56-57 0.4433828748910855 1105 58-59 0.4475742995043106 1105 60-61 0.5844612010985273 1105 62-63 0.44137509629752003 1105 64-65 0.3514347485494582 1105 66-67 0.36869453862947665 1105 68-69 0.3708188812192077 1105 70-71 0.37587287708297623 1105 72-73 0.4799039693159486 1105 74-75 0.41178200452719693 1105 76-77 0.2717706976093126 1105 78-79 0.49762882147276244 1105 80-81 0.613738688271102 1105 82-83 0.8082977461240866 1105 84-85 0.7180548310774348 1105 86-87 0.6105771753717697 1105 88-89 0.6328156066196158 1105 90-91 0.8853635047511652 1105 92-93 0.9331763021407298 1105 94-95 0.9934451013551104 1105 96-97 0.9537014805241419 1105 98-99 0.9105696912601466 1105 100-101 0.8514920914805586 1106 1 0.25232978292277863 1106 2 0.2046748121897224 1106 3 0.16792176353813915 1106 4 0.19808061027278967 1106 5 0.19535625002178136 1106 6 0.2522508910686483 1106 7 0.2801086376034405 1106 8 0.2612248282383476 1106 9 0.4021341799795195 1106 10-11 0.32953613631423195 1106 12-13 0.3942315959249143 1106 14-15 0.5243127881788539 1106 16-17 0.5431904266440881 1106 18-19 0.5208599142200541 1106 20-21 0.4077263338025858 1106 22-23 0.4783970884851314 1106 24-25 0.5354830604144496 1106 26-27 0.5550954155526924 1106 28-29 0.6296488482820237 1106 30-31 0.66584339559558 1106 32-33 0.6602540036397713 1106 34-35 0.5927170288905188 1106 36-37 0.6617713922228248 1106 38-39 0.7181938882297558 1106 40-41 0.5978591357289886 1106 42-43 0.5277677749813421 1106 44-45 0.6693704283127815 1106 46-47 0.4591856921300135 1106 48-49 0.3875529557045425 1106 50-51 0.34382463579088807 1106 52-53 0.32662421390455165 1106 54-55 0.4765687025783407 1106 56-57 0.39739366672787213 1106 58-59 0.39328113634894635 1106 60-61 0.382419092814402 1106 62-63 0.5062101480087335 1106 64-65 0.5811630107541248 1106 66-67 0.6809190740318556 1106 68-69 0.6745122270980488 1106 70-71 0.6014433615107393 1106 72-73 0.7467786922184914 1106 74-75 0.7145158182342684 1106 76-77 0.5418524396510378 1106 78-79 0.6459181188140697 1106 80-81 0.6881374769774986 1106 82-83 0.8900400802974708 1106 84-85 0.7832957074793967 1106 86-87 0.6519612530062382 1106 88-89 0.9736324070404336 1106 90-91 1.0624917983845812 1106 92-93 1.2404419386255299 1106 94-95 1.3090745478308996 1106 96-97 1.4741613618825653 1106 98-99 1.2894185918973378 1106 100-101 1.3960615468702535 1107 1 0.1803984667333438 1107 2 0.11115237246155374 1107 3 0.096886534879566 1107 4 0.09852418983404476 1107 5 0.18630162642521242 1107 6 0.09591588095316439 1107 7 0.155172404701851 1107 8 0.17142208518779256 1107 9 0.18173662230504561 1107 10-11 0.21860299588863086 1107 12-13 0.23773820802006185 1107 14-15 0.2792153975061993 1107 16-17 0.26101924064708015 1107 18-19 0.3893906228718791 1107 20-21 0.2106552575844134 1107 22-23 0.2513063697685496 1107 24-25 0.21880388044817067 1107 26-27 0.23587245087469455 1107 28-29 0.2864480341735174 1107 30-31 0.22405499481556035 1107 32-33 0.2670148379589534 1107 34-35 0.21408768161803948 1107 36-37 0.2579413079508299 1107 38-39 0.11747748491731613 1107 40-41 0.16921020683361832 1107 42-43 0.2785076153657542 1107 44-45 0.2555508755042908 1107 46-47 0.317401045339885 1107 48-49 0.18902188179963986 1107 50-51 0.21476489865134596 1107 52-53 0.32130318689409165 1107 54-55 0.26431862191828515 1107 56-57 0.3846573936484461 1107 58-59 0.2222871001198996 1107 60-61 0.14973026205035467 1107 62-63 0.21666125584158635 1107 64-65 0.10992902355015843 1107 66-67 -0.0031883590710322096 1107 68-69 0.2677235929619002 1107 70-71 0.19699944916335 1107 72-73 0.21056669122014426 1107 74-75 0.3303748021408097 1107 76-77 0.30608036568322916 1107 78-79 0.29478309490013643 1107 80-81 0.3609433713503698 1107 82-83 0.3442605795172504 1107 84-85 0.3952235537646729 1107 86-87 0.49012279226384337 1107 88-89 0.34110449054540126 1107 90-91 0.4957978053771477 1107 92-93 0.5988760378404407 1107 94-95 0.5996305238784139 1107 96-97 0.669130812521658 1107 98-99 0.8788196580304621 1107 100-101 1.0535145357564168 1108 1 -0.05164733413744216 1108 2 -0.17161626567920862 1108 3 -0.10968486261191401 1108 4 0.010414920057371546 1108 5 -0.009353579968475856 1108 6 -0.027971891190610165 1108 7 0.011329296268492328 1108 8 -0.0093917157707466 1108 9 0.06399920579811891 1108 10-11 0.024830228685651434 1108 12-13 0.05627904112715498 1108 14-15 0.010461529652651791 1108 16-17 0.06024461870263309 1108 18-19 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-1.1766048522708203 2316 58-59 -1.1537163862230813 2316 60-61 -0.597699908769286 2316 62-63 -0.718489881033662 2316 64-65 -0.9252838777792647 2316 66-67 -1.0035716893657494 2316 68-69 -0.9613999285049601 2316 70-71 -0.8915252919654506 2316 72-73 -0.7393038266710903 2316 74-75 -0.84452306326477 2316 76-77 -1.0261809574899736 2316 78-79 -0.8025996363537935 2316 80-81 -1.0618956169032252 2316 82-83 -0.8631676193047575 2316 84-85 -0.7970219376162113 2316 86-87 -0.8091263603352878 2316 88-89 -0.8163464048531495 2316 90-91 -1.019330114016519 2316 92-93 -1.2163490861535635 2316 94-95 -1.056193845547437 2316 96-97 -0.8595246385634958 2316 98-99 -0.728030708277398 2316 100-101 -0.5548806860561086 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 17.0 5 36.0 6 80.0 7 238.0 8 537.0 9 1060.0 10 1740.0 11 2873.0 12 4338.0 13 5816.0 14 7484.0 15 10244.0 16 13164.0 17 17898.0 18 16711.0 19 11448.0 20 10710.0 21 11001.0 22 11752.0 23 13141.0 24 15777.0 25 18609.0 26 22789.0 27 28967.0 28 36048.0 29 45169.0 30 56511.0 31 71174.0 32 91433.0 33 117518.0 34 159947.0 35 225823.0 36 342564.0 37 499554.0 38 345774.0 39 26166.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.60246614916784 19.48125920059401 15.140963239518287 22.775311410719866 2 15.815294091958132 20.61390980475864 39.67664612487372 23.894149978409505 3 19.735148739868773 24.351619302939504 29.532203353576474 26.38102860361525 4 13.673815144863408 17.203003055994483 36.44663328155343 32.67654851758868 5 14.367892666593288 37.02531067393976 33.833747832623985 14.773048826842977 6 32.26591590066075 36.02580624461924 17.22247770606406 14.485800148655947 7 28.62975888946917 32.27547060847122 20.691978489525496 18.40279201253411 8 26.293872509264226 35.16556185152639 19.500640786839895 19.039924852369484 9 26.068928700655402 17.121262893718505 20.211700286437956 36.59810811918813 10-11 25.01129064492921 26.70763902644005 27.11220840113105 21.168861927499687 12-13 25.948070438115987 23.86395867380985 27.75763976386944 22.43033112420472 14-15 22.995847808223818 25.321752529726627 25.891887941621555 25.790511720428 16-17 22.448884104030274 28.372218726661192 26.563020806411075 22.61587636289746 18-19 22.375692700377343 27.090756449310106 28.71150600058108 21.822044849731476 20-21 23.20872937022753 26.18719715836032 28.84535215579465 21.7587213156175 22-23 22.845075744747597 26.096066335251834 28.77471574553187 22.2841421744687 24-25 22.772062355783756 26.26544879604782 28.858890226762078 22.10359862140635 26-27 22.613789063692373 26.358738821398838 28.79515417373435 22.23231794117444 28-29 22.508057427483152 26.52292342296676 28.789698079029076 22.17932107052101 30-31 22.725065609340696 26.28984164399838 28.684713497151115 22.30037924950981 32-33 22.64469262092365 26.338664641803266 28.676303483531495 22.340339253741586 34-35 22.855522074662687 26.39608193341907 28.580487383169658 22.167908608748586 36-37 22.88444077543786 26.23977700758348 28.608759493276025 22.267022723702638 38-39 22.75206545279538 26.420839906241365 28.66088696380667 22.16620767715658 40-41 22.887371958898505 26.386181676876085 28.499322194325682 22.227124169899724 42-43 22.70040979442544 26.441677822082553 28.321202004231633 22.536710379260374 44-45 22.905401972990582 26.431193535421233 28.25093823553789 22.412466256050294 46-47 23.07484756236814 26.71813314338462 27.846195013667973 22.360824280579266 48-49 23.12390755811039 26.76311905040411 27.64816826250912 22.46480512897638 50-51 22.906828423263097 27.014955801012654 27.644447415180835 22.43376836054342 52-53 23.16910711448897 27.022206556378432 27.326762145627438 22.481924183505157 54-55 23.07451670324132 26.868699562240522 27.403783487864857 22.653000246653303 56-57 23.049533354960374 27.125168887003454 27.31072178473012 22.51457597330605 58-59 23.186085363152788 26.927323989353336 27.383367727486014 22.503222920007858 60-61 23.14794555830896 26.8373714233259 27.679138394243726 22.335544624121415 62-63 22.96169181985245 26.875727457938897 27.732211703851316 22.43036901835734 64-65 22.98187254635468 26.954583331476623 27.49212382637215 22.57142029579655 66-67 22.953886711217276 27.023821645464167 27.34515106069677 22.67714058262179 68-69 22.865284147521788 26.872340823736383 27.578387213495205 22.683987815246628 70-71 22.992017107884347 26.92236994463065 27.38736466525839 22.69824828222662 72-73 23.065083563008134 26.867562491227055 27.377585373298608 22.689768572466207 74-75 22.95119880754243 27.215281816136056 27.08678961100305 22.74672976531847 76-77 23.126961948913628 27.057688789923272 27.118537196289914 22.69681206487318 78-79 23.056613509759543 27.113520677007507 27.040084024364212 22.789781788868734 80-81 22.972719441670357 27.189247314703035 27.085391619083783 22.752641624542832 82-83 23.05687539061238 27.219864777096102 27.076197404495623 22.647062427795895 84-85 23.01350872001825 27.177330804551808 27.0237679320081 22.78539254342184 86-87 22.930394981082358 27.194389478578984 27.086817052680413 22.78839848765825 88-89 23.116673677681092 27.07246774636289 26.914296587299607 22.896561988656416 90-91 23.07394948471846 27.013858036560546 27.08259343446609 22.829599044254902 92-93 22.96216482618653 27.07750559184677 27.06313467377236 22.897194908194344 94-95 22.974377849956753 27.05004159772703 27.03836660631617 22.937213946000046 96-97 23.017870161260134 27.048994775578013 27.022992982412358 22.910142080749495 98-99 22.85997129293638 27.100836123307577 26.8183999711236 23.220792612632447 100-101 22.76238504841814 27.232156185762022 26.851490822366415 23.153967943453424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 401.0 1 310.5 2 243.5 3 278.0 4 240.0 5 220.0 6 421.0 7 993.0 8 1706.0 9 2107.5 10 1986.0 11 1741.5 12 1783.5 13 1977.5 14 2204.5 15 2372.5 16 2498.5 17 2622.0 18 2766.0 19 3022.0 20 3328.0 21 3772.5 22 4538.5 23 5679.0 24 6956.0 25 8590.5 26 10990.5 27 13967.5 28 16842.0 29 19884.0 30 23791.5 31 27938.0 32 31761.0 33 35580.5 34 40088.0 35 44259.0 36 49210.5 37 54352.0 38 57916.5 39 60606.5 40 62921.5 41 65959.0 42 68418.5 43 72214.0 44 77864.0 45 81493.0 46 83642.5 47 86112.5 48 89819.0 49 90218.0 50 87029.0 51 84855.0 52 83675.0 53 82689.5 54 80410.5 55 75622.0 56 69149.0 57 62987.0 58 57770.5 59 51484.5 60 43110.0 61 34638.5 62 27191.0 63 21037.5 64 15703.0 65 11245.0 66 8143.5 67 6123.0 68 4388.5 69 2972.0 70 2023.5 71 1266.0 72 788.0 73 563.5 74 362.0 75 203.0 76 115.0 77 61.5 78 34.0 79 16.0 80 10.0 81 13.0 82 9.5 83 5.5 84 4.0 85 1.5 86 1.5 87 1.5 88 1.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016710366130806075 2 0.003252951273463582 3 0.0028519024863242364 4 8.912195269763239E-5 5 0.0019161219829990964 6 3.5648781079052955E-4 7 0.0 8 0.0 9 0.0 10-11 6.68414645232243E-5 12-13 8.243780624530996E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0026513780927545634 22-23 0.0 24-25 0.0040104878713934575 26-27 0.0018047195421270557 28-29 0.0030524268798939095 30-31 0.015262134399469546 32-33 6.238536688834267E-4 34-35 0.0022280488174408098 36-37 0.004166451288614315 38-39 0.0024954146755337067 40-41 0.0049017073983697815 42-43 0.0028519024863242364 44-45 4.4560976348816193E-4 46-47 6.68414645232243E-5 48-49 1.5596341722085667E-4 50-51 1.5596341722085667E-4 52-53 0.001180865873243629 54-55 0.0033643537143356224 56-57 5.347317161857944E-4 58-59 0.0014705122195109344 60-61 0.0012031463614180372 62-63 0.0 64-65 2.673658580928972E-4 66-67 6.68414645232243E-5 68-69 0.0 70-71 2.0052439356967288E-4 72-73 4.4560976348816194E-5 74-75 4.901707398369782E-4 76-77 6.461341570578349E-4 78-79 2.2280488174408096E-4 80-81 0.006104853759787819 82-83 0.0019384024711735044 84-85 0.00374312201330056 86-87 0.002072085400219953 88-89 0.0012477073377668534 90-91 1.336829290464486E-4 92-93 2.2280488174408097E-5 94-95 1.336829290464486E-4 96-97 0.0017824390539526477 98-99 0.0030747073680683175 100-101 8.466585506275077E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2244116.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.46498754553134 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36696086800862 46.60090351997275 2 12.521387327210713 14.891682829307182 3 3.799593614936246 6.778283609707929 4 1.6506004001554806 3.9261172895157888 5 0.9064183775204938 2.695007876514845 6 0.585541857062868 2.0891543542578446 7 0.39621250951030246 1.64925403603897 8 0.27990425218801207 1.3315602296241122 9 0.21977940314948374 1.1762261523943527 >10 1.1834595375937733 12.632757857661408 >50 0.06687417291119441 2.6927463944069117 >100 0.02214324342344999 2.294407489208548 >500 7.496242195805594E-4 0.28439739934194413 >1k 2.2488726587405338E-4 0.3405636568920925 >5k 1.499248439160356E-4 0.6169373051553089 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7651 0.3409360300447927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6189 0.27578788262282344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4103 0.18283368595919283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4560976348816194E-5 2 0.0 0.0 0.0 8.912195269763239E-5 4.4560976348816194E-5 3 0.0 0.0 0.0 8.912195269763239E-5 4.4560976348816194E-5 4 0.0 0.0 0.0 8.912195269763239E-5 4.4560976348816194E-5 5 0.0 4.4560976348816194E-5 0.0 8.912195269763239E-5 4.4560976348816194E-5 6 4.4560976348816194E-5 4.4560976348816194E-5 0.0 8.912195269763239E-5 4.4560976348816194E-5 7 4.4560976348816194E-5 4.4560976348816194E-5 0.0 8.912195269763239E-5 4.4560976348816194E-5 8 4.4560976348816194E-5 4.4560976348816194E-5 0.0 8.912195269763239E-5 4.4560976348816194E-5 9 4.4560976348816194E-5 4.4560976348816194E-5 0.0 8.912195269763239E-5 8.912195269763239E-5 10-11 4.4560976348816194E-5 4.4560976348816194E-5 0.0 8.912195269763239E-5 8.912195269763239E-5 12-13 4.4560976348816194E-5 4.4560976348816194E-5 0.0 1.7824390539526478E-4 8.912195269763239E-5 14-15 4.4560976348816194E-5 8.912195269763239E-5 0.0 2.2280488174408096E-4 8.912195269763239E-5 16-17 4.4560976348816194E-5 1.336829290464486E-4 0.0 2.450853699184891E-4 8.912195269763239E-5 18-19 4.4560976348816194E-5 1.336829290464486E-4 0.0 2.673658580928972E-4 8.912195269763239E-5 20-21 4.4560976348816194E-5 1.336829290464486E-4 0.0 2.673658580928972E-4 8.912195269763239E-5 22-23 4.4560976348816194E-5 1.336829290464486E-4 0.0 3.1192683444171334E-4 8.912195269763239E-5 24-25 4.4560976348816194E-5 1.5596341722085667E-4 0.0 4.4560976348816193E-4 8.912195269763239E-5 26-27 4.4560976348816194E-5 1.7824390539526478E-4 0.0 6.684146452322428E-4 8.912195269763239E-5 28-29 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.0012699878259412615 8.912195269763239E-5 30-31 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.0024062927228360744 8.912195269763239E-5 32-33 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.0037876829896493765 8.912195269763239E-5 34-35 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.005169073256462678 8.912195269763239E-5 36-37 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.007152036703984999 8.912195269763239E-5 38-39 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.010694634323715885 1.336829290464486E-4 40-41 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.014326353896144405 1.336829290464486E-4 42-43 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.0173342197996895 1.5596341722085667E-4 44-45 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.01956226861713031 1.7824390539526478E-4 46-47 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.023015744284163565 1.7824390539526478E-4 48-49 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.02588992725866221 1.7824390539526478E-4 50-51 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.029187439508474605 1.7824390539526478E-4 52-53 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.0371415737867383 2.450853699184891E-4 54-55 4.4560976348816194E-5 1.7824390539526478E-4 0.0 0.04857146422020965 2.8964634626730526E-4 56-57 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.06641813524791053 3.1192683444171334E-4 58-59 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.0897235258783414 3.1192683444171334E-4 60-61 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.11494503849177137 3.1192683444171334E-4 62-63 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.13005120947402005 3.5648781079052955E-4 64-65 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.14881138051687168 3.5648781079052955E-4 66-67 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.18806960068017875 3.5648781079052955E-4 68-69 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.24403818697429186 3.5648781079052955E-4 70-71 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.29771188298644097 3.5648781079052955E-4 72-73 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.3634838840772937 4.010487871393457E-4 74-75 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.427651690019589 4.2332927531375385E-4 76-77 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.4796097884423087 4.4560976348816193E-4 78-79 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.528381777056088 4.4560976348816193E-4 80-81 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.5821445950209347 4.6789025166257E-4 82-83 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.6477160717182178 5.347317161857944E-4 84-85 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.7720411957314149 5.347317161857944E-4 86-87 8.912195269763239E-5 1.7824390539526478E-4 0.0 0.9531147231248296 5.347317161857944E-4 88-89 8.912195269763239E-5 1.7824390539526478E-4 0.0 1.154107006946165 5.347317161857944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6295 0.0 37.35703 1 GTATCAA 9160 0.0 31.533415 1 ATCAACG 11235 0.0 25.449535 3 TCAACGC 11380 0.0 25.125263 4 TATCAAC 11520 0.0 24.985397 2 CAACGCA 11550 0.0 24.755457 5 AACGCAG 11865 0.0 24.098768 6 GAGTACT 7380 0.0 21.88184 12-13 TACATGG 7500 0.0 21.5953 2 GTACATG 7675 0.0 21.540895 1 ACGCAGA 13365 0.0 21.216389 7 CGCAGAG 13405 0.0 20.940487 8 GTACTTT 7655 0.0 20.786213 14-15 CGTATAC 140 4.8565227E-4 20.3554 3 ACATGGG 7720 0.0 20.302666 3 AGAGTAC 11495 0.0 19.378939 10-11 ACCGTAC 150 7.7274814E-4 18.998796 8 AGTACTT 7685 0.0 18.850254 12-13 GCAGAGT 14710 0.0 18.598413 9 GACCGTA 185 1.5228921E-4 17.971834 7 >>END_MODULE