FastQCFastQC Report
Thu 26 May 2016
SRR936998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098832
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68160.3247520525701914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50710.24161057197527006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.15399040990417528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53150.034.0652431
GTATCAA82800.033.460161
ATCAACG99550.027.7811873
TCAACGC99850.027.69974
TATCAAC100650.027.4788762
CAACGCA101450.027.266745
AACGCAG103250.026.9282026
GTACATG71550.025.504181
TACATGG69650.025.2447092
ACATGGG71350.023.6430473
ACGCAGA117300.023.215477
CGCAGAG117150.023.0017918
GAGTACT60200.021.70753112-13
GCAGAGT125850.020.5053639
GTACTTT66500.019.61506714-15
AGAGTAC96700.019.53534910-11
GTATAGG6750.019.0085871
CATGGGG44700.018.9239864
AGTACTT66150.018.78521212-13
CATGGGA32600.018.2218554
Produced by FastQC (version 0.11.5)