##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936998_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2098832 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54133060673746 31.0 31.0 34.0 30.0 34.0 2 31.674610926458143 33.0 31.0 34.0 30.0 34.0 3 31.687906892976667 33.0 31.0 34.0 30.0 34.0 4 35.27081776912111 37.0 35.0 37.0 33.0 37.0 5 35.26614707608803 37.0 35.0 37.0 33.0 37.0 6 35.31522246659094 37.0 35.0 37.0 33.0 37.0 7 35.24688541055215 37.0 35.0 37.0 32.0 37.0 8 35.19262951965665 37.0 35.0 37.0 32.0 37.0 9 36.79796381987696 39.0 37.0 39.0 33.0 39.0 10-11 36.60407336080258 39.0 37.0 39.0 32.0 39.0 12-13 36.51846050565267 39.0 37.0 39.0 32.0 39.0 14-15 37.641758844919465 40.0 37.0 41.0 32.0 41.0 16-17 37.56183343878881 40.0 37.0 41.0 32.0 41.0 18-19 37.50264456612059 40.0 37.0 41.0 32.0 41.0 20-21 37.407777278028924 40.0 37.0 41.0 32.0 41.0 22-23 37.288684611250446 40.0 37.0 41.0 31.5 41.0 24-25 37.114125380211476 39.5 36.5 41.0 31.0 41.0 26-27 37.215250910982874 40.0 37.0 41.0 31.0 41.0 28-29 37.17305696692256 40.0 37.0 41.0 31.0 41.0 30-31 37.03375091479451 39.5 36.5 41.0 30.5 41.0 32-33 36.82944347141648 39.0 36.0 41.0 30.0 41.0 34-35 36.654025191153934 39.0 36.0 41.0 30.0 41.0 36-37 36.48179463625483 39.0 35.5 41.0 30.0 41.0 38-39 36.313998214244876 39.0 35.0 41.0 30.0 41.0 40-41 36.137082910876146 39.0 35.0 41.0 29.5 41.0 42-43 35.92439723617707 39.0 35.0 40.0 28.5 41.0 44-45 35.681089053340145 39.0 35.0 40.0 27.5 41.0 46-47 35.36276581451017 38.0 35.0 40.0 26.5 41.0 48-49 35.051488637489804 38.0 34.0 40.0 25.5 41.0 50-51 34.41804918164007 37.5 33.5 39.5 24.5 40.5 52-53 34.663343707357235 38.0 34.0 39.5 25.5 40.5 54-55 35.033704460385586 39.0 34.5 40.0 26.0 41.0 56-57 34.90825659223797 38.0 34.0 40.0 25.5 41.0 58-59 34.63881768526495 38.0 34.0 40.0 25.0 41.0 60-61 34.33210280765683 38.0 34.0 40.0 24.0 41.0 62-63 33.99178543113503 37.5 33.5 40.0 23.0 41.0 64-65 33.65334814792227 37.0 33.0 40.0 22.0 41.0 66-67 33.250804018616066 36.0 33.0 39.0 22.0 41.0 68-69 32.847873483918676 36.0 32.0 39.0 20.5 41.0 70-71 32.41735593892222 35.0 32.0 38.5 19.5 40.0 72-73 32.009270155972466 35.0 31.5 38.0 18.0 40.0 74-75 31.564281467025467 35.0 31.0 37.0 17.0 39.0 76-77 31.122601523132865 35.0 31.0 36.5 12.5 39.0 78-79 30.663488311594257 35.0 30.5 36.0 8.0 39.0 80-81 30.229021188927938 34.0 30.0 36.0 7.0 37.5 82-83 29.810963192861553 34.0 30.0 35.0 4.5 37.0 84-85 29.439925873057014 34.0 29.0 35.0 2.0 36.5 86-87 29.09441370247833 34.0 29.0 35.0 2.0 36.0 88-89 28.726403542541757 34.0 29.0 35.0 2.0 36.0 90-91 28.35854703949625 34.0 29.0 35.0 2.0 35.5 92-93 27.966618814655007 34.0 27.5 35.0 2.0 35.0 94-95 27.511032564778887 34.0 27.0 35.0 2.0 35.0 96-97 26.997035017571676 33.5 25.5 35.0 2.0 35.0 98-99 26.332309351105756 33.0 25.0 35.0 2.0 35.0 100-101 24.024947208733238 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3430305492109085 1101 2 0.4117440681164872 1101 3 0.30566016212865676 1101 4 0.31355349464363513 1101 5 0.2700585976492249 1101 6 0.2263693762437029 1101 7 0.1904316840747171 1101 8 0.2691448782616135 1101 9 0.08882321586840902 1101 10-11 0.24940007233107764 1101 12-13 0.10705387160442115 1101 14-15 0.4473380246822529 1101 16-17 0.617081658200803 1101 18-19 0.4811359748415214 1101 20-21 0.36525294493775107 1101 22-23 0.48639777721921007 1101 24-25 0.3539079986172382 1101 26-27 0.5766200710925133 1101 28-29 0.5552651004668476 1101 30-31 0.4489632102229848 1101 32-33 0.53130072922972 1101 34-35 0.4560651353801859 1101 36-37 0.27228914805294124 1101 38-39 0.32512026665533966 1101 40-41 0.480428913900667 1101 42-43 0.44532564529080076 1101 44-45 0.5062045441541869 1101 46-47 0.3880004079195558 1101 48-49 0.34868388513226733 1101 50-51 0.314872438835998 1101 52-53 0.4074121459394746 1101 54-55 0.47972875653724145 1101 56-57 0.48226848877935424 1101 58-59 0.43679901097262785 1101 60-61 0.5655249416193939 1101 62-63 0.4588546910552651 1101 64-65 0.600184868786279 1101 66-67 0.6175212884252304 1101 68-69 0.5838967195318716 1101 70-71 0.6367037101205995 1101 72-73 0.7149292669406364 1101 74-75 0.5963580051552277 1101 76-77 0.6686016289655647 1101 78-79 0.8351275627901558 1101 80-81 0.524169410119665 1101 82-83 0.5972372586187547 1101 84-85 0.6487012932815972 1101 86-87 0.6474844256143086 1101 88-89 0.719854358890931 1101 90-91 0.8022302621510136 1101 92-93 0.9849119330170986 1101 94-95 0.9189976730468281 1101 96-97 0.7772993833121546 1101 98-99 0.9355451362818101 1101 100-101 0.9839234820516118 1102 1 0.44073800817284337 1102 2 0.3568389458530312 1102 3 0.41806853880920514 1102 4 0.4316645283329308 1102 5 0.1614677467474408 1102 6 0.17955911011070214 1102 7 0.24584387422989806 1102 8 0.2604943405400846 1102 9 0.2690841634034271 1102 10-11 0.5226033235072549 1102 12-13 0.40866912928404275 1102 14-15 0.44804528648862885 1102 16-17 0.5245174611100651 1102 18-19 0.3097794365690376 1102 20-21 0.4919162761644955 1102 22-23 0.521386136938979 1102 24-25 0.6006471672149516 1102 26-27 0.448728906362291 1102 28-29 0.49536559578643136 1102 30-31 0.5810044319392631 1102 32-33 0.6715185050989589 1102 34-35 0.4774865594374873 1102 36-37 0.29817087902473105 1102 38-39 0.3624096748105572 1102 40-41 0.3685029606670511 1102 42-43 0.3730178314488626 1102 44-45 0.36284308239426366 1102 46-47 0.3394157417606323 1102 48-49 0.49754342124954576 1102 50-51 0.39584193103890186 1102 52-53 0.42087577749084915 1102 54-55 0.4157089032425887 1102 56-57 0.6052169231481628 1102 58-59 0.5487195626841057 1102 60-61 0.5215849093182925 1102 62-63 0.47660611840748857 1102 64-65 0.5332603469560198 1102 66-67 0.3003077851600082 1102 68-69 0.10026535721397067 1102 70-71 0.3029483828693742 1102 72-73 0.353479660441522 1102 74-75 0.2826548160965565 1102 76-77 0.28558408212086306 1102 78-79 0.4608776991785888 1102 80-81 0.4037829852251278 1102 82-83 0.262595744139972 1102 84-85 0.26759824844356217 1102 86-87 0.1786377046083274 1102 88-89 0.11058055303243819 1102 90-91 0.1456836848255243 1102 92-93 0.4850439327146141 1102 94-95 0.4029740575919938 1102 96-97 0.7361151400924157 1102 98-99 0.7611792749820943 1102 100-101 0.7950268509809781 1103 1 0.3343152196642265 1103 2 0.4100692538849593 1103 3 0.39485034880982184 1103 4 0.3478522111523432 1103 5 0.2678181838621896 1103 6 0.14943301385493868 1103 7 0.223246617768325 1103 8 0.2621904686502816 1103 9 0.355588679253664 1103 10-11 0.4386726506789387 1103 12-13 0.34003383845456625 1103 14-15 0.506464246650431 1103 16-17 0.45066006801998526 1103 18-19 0.36716433343030275 1103 20-21 0.5779084681606435 1103 22-23 0.6192623757501181 1103 24-25 0.5800105013786165 1103 26-27 0.6119753728721804 1103 28-29 0.5375742922257487 1103 30-31 0.5903451951110199 1103 32-33 0.4977054313672724 1103 34-35 0.5386803937772413 1103 36-37 0.46536569985426723 1103 38-39 0.53325857140225 1103 40-41 0.5910063750581713 1103 42-43 0.5907399419687565 1103 44-45 0.7615053744996345 1103 46-47 0.586531514562715 1103 48-49 0.648352303225586 1103 50-51 0.5109794284207041 1103 52-53 0.36653550326915507 1103 54-55 0.4739734146526331 1103 56-57 0.6174068941217072 1103 58-59 0.7035590615235066 1103 60-61 0.6403101603511985 1103 62-63 0.602503820804742 1103 64-65 0.5774685352837352 1103 66-67 0.6381349439571053 1103 68-69 0.5916936992702162 1103 70-71 0.39062993035666693 1103 72-73 0.35099526554695615 1103 74-75 0.3802477612079471 1103 76-77 0.4318226851244731 1103 78-79 0.3687199460494739 1103 80-81 0.39147069414390856 1103 82-83 0.4458386987991041 1103 84-85 0.4335511196045587 1103 86-87 0.5414155223977843 1103 88-89 0.44416896081943236 1103 90-91 0.6031731112804977 1103 92-93 0.542503741350135 1103 94-95 0.5304314446032308 1103 96-97 0.7127015352552419 1103 98-99 0.6844357550570486 1103 100-101 0.751199250807332 1104 1 0.33613842347731193 1104 2 0.3589333459890405 1104 3 0.39019132752879315 1104 4 0.41860969276138604 1104 5 0.326101629817245 1104 6 0.22635271134555524 1104 7 0.336821931096118 1104 8 0.3038051456073916 1104 9 0.3181124885714439 1104 10-11 0.5226362118972219 1104 12-13 0.5632841968216127 1104 14-15 0.5684933646597798 1104 16-17 0.5320558174056771 1104 18-19 0.4861320432859557 1104 20-21 0.5130503555526857 1104 22-23 0.3668805287095225 1104 24-25 0.5262711696004558 1104 26-27 0.2893131458410423 1104 28-29 0.332103748882389 1104 30-31 0.5289800587751117 1104 32-33 0.6093589420996466 1104 34-35 0.47969885180211946 1104 36-37 0.390621998444324 1104 38-39 0.45158915839200375 1104 40-41 0.42801559857903015 1104 42-43 0.4133091450010369 1104 44-45 0.5013457060444892 1104 46-47 0.5413705264647959 1104 48-49 0.6198426941294102 1104 50-51 0.5181654907298068 1104 52-53 0.4906728679308827 1104 54-55 0.5883864432045129 1104 56-57 0.40986477788064946 1104 58-59 0.4596569593808226 1104 60-61 0.5675080644988739 1104 62-63 0.6401153871990743 1104 64-65 0.615474912836028 1104 66-67 0.483276861303203 1104 68-69 0.45537260372375243 1104 70-71 0.5705372624783109 1104 72-73 0.7121105184540824 1104 74-75 0.5927639988048874 1104 76-77 0.47919128747799533 1104 78-79 0.7251042047247509 1104 80-81 0.6596094586587284 1104 82-83 0.6841142635080288 1104 84-85 0.8139562050052511 1104 86-87 0.6574241871090081 1104 88-89 0.6504782178555537 1104 90-91 0.6461255698598549 1104 92-93 0.676681207842357 1104 94-95 0.8004007350348061 1104 96-97 0.924902256748716 1104 98-99 1.1513674707823647 1104 100-101 0.9584221066873653 1105 1 0.16157473385008458 1105 2 0.17237208337981258 1105 3 0.26641200980918 1105 4 0.12532962132251413 1105 5 0.24473879062608006 1105 6 0.24180850504622242 1105 7 0.3025483499008885 1105 8 0.33878940638165744 1105 9 0.2880183051566263 1105 10-11 0.37583906385332 1105 12-13 0.31367435326210114 1105 14-15 0.4019742459444515 1105 16-17 0.3990304918787331 1105 18-19 0.19416835617813177 1105 20-21 0.43786685955502236 1105 22-23 0.33710266944017775 1105 24-25 0.38013728750538434 1105 26-27 0.3861380772224976 1105 28-29 0.19861863361303023 1105 30-31 -0.15974211064865784 1105 32-33 -0.029842577375980284 1105 34-35 0.048848044825625436 1105 36-37 -0.03355415911308768 1105 38-39 -0.15351303810128059 1105 40-41 0.108085917716501 1105 42-43 0.17149245764266396 1105 44-45 0.21048145529181994 1105 46-47 0.012966087849179075 1105 48-49 0.09326656708467596 1105 50-51 -0.012132693765543934 1105 52-53 -0.12428454028263047 1105 54-55 -0.055509200620100785 1105 56-57 0.09120214318045328 1105 58-59 -0.021781216744535925 1105 60-61 0.033659106478999945 1105 62-63 0.18525228837017238 1105 64-65 0.1243003215030214 1105 66-67 0.22385292116405964 1105 68-69 0.2616060439447878 1105 70-71 0.14653956212684704 1105 72-73 0.16399618127213245 1105 74-75 0.25274511033434166 1105 76-77 0.3251477271237704 1105 78-79 0.3746719883702312 1105 80-81 0.36282095354225774 1105 82-83 0.3635942742014677 1105 84-85 0.2608039509157791 1105 86-87 0.21141851426935432 1105 88-89 0.31701973120065574 1105 90-91 0.38208571161786153 1105 92-93 0.2517214132011141 1105 94-95 0.4055638169277671 1105 96-97 0.4694150825730823 1105 98-99 0.6026124372394115 1105 100-101 0.7661626355085538 1106 1 0.19838458972569128 1106 2 0.24160558919193065 1106 3 0.28020910765353335 1106 4 0.11852760363886006 1106 5 0.10630431762156434 1106 6 0.1656948888631078 1106 7 0.298002343184919 1106 8 0.2869214003771958 1106 9 0.2128779552435418 1106 10-11 0.40736717866692373 1106 12-13 0.39433614540249806 1106 14-15 0.44198517892067457 1106 16-17 0.4796720377668606 1106 18-19 0.45192917852305214 1106 20-21 0.4439805046613543 1106 22-23 0.2004335973259188 1106 24-25 0.36442077263740913 1106 26-27 0.2871187189129998 1106 28-29 0.3024110458961431 1106 30-31 0.441044456252186 1106 32-33 0.4471735878102976 1106 34-35 0.33266434768642483 1106 36-37 0.35220021624664355 1106 38-39 0.4052249551480429 1106 40-41 0.28829889382881646 1106 42-43 0.2579479317954352 1106 44-45 0.3453981419637131 1106 46-47 0.1205879341338445 1106 48-49 0.270707614336537 1106 50-51 0.27859481192509605 1106 52-53 0.32856229971124407 1106 54-55 0.3967071132315212 1106 56-57 0.35162679386029083 1106 58-59 0.3225619803700752 1106 60-61 0.36457939987612065 1106 62-63 0.337874684195981 1106 64-65 0.32311562651280923 1106 66-67 0.48495112127222484 1106 68-69 0.38282397171194305 1106 70-71 0.3024841510267038 1106 72-73 0.5515369268548369 1106 74-75 0.4785233618101472 1106 76-77 0.4345132818855433 1106 78-79 0.34634278141593455 1106 80-81 0.4673350001028531 1106 82-83 0.5982946152061999 1106 84-85 0.718965432131597 1106 86-87 0.610292461102695 1106 88-89 0.625155759985045 1106 90-91 0.46853323188580376 1106 92-93 0.4151393999716326 1106 94-95 0.46040079121213395 1106 96-97 0.4175405688446787 1106 98-99 0.4595104800052816 1106 100-101 0.20216061666797103 1107 1 0.2554717446923256 1107 2 0.26241162514456207 1107 3 0.2040476662487194 1107 4 0.24866733676714858 1107 5 0.24052899099206115 1107 6 0.16356946784819115 1107 7 0.2611528126078042 1107 8 0.3273972015484148 1107 9 0.37235302799359005 1107 10-11 0.4482398736263775 1107 12-13 0.3747928779953398 1107 14-15 0.3158125423674818 1107 16-17 0.4480951827050035 1107 18-19 0.3360059949174641 1107 20-21 0.5471004341613863 1107 22-23 0.4891694551652108 1107 24-25 0.5050128707776125 1107 26-27 0.2846254046998027 1107 28-29 0.32509383431299455 1107 30-31 0.27377577416419996 1107 32-33 0.2476898535507175 1107 34-35 0.35971213918562484 1107 36-37 0.3847232270639509 1107 38-39 0.04295382043873275 1107 40-41 0.5150532177707419 1107 42-43 0.48112229402182294 1107 44-45 0.4434974709176771 1107 46-47 0.18892437191128408 1107 48-49 0.41207103610417306 1107 50-51 0.3134732230389332 1107 52-53 0.22930000106740067 1107 54-55 0.261939346273266 1107 56-57 0.34658013881595195 1107 58-59 0.5444891330976844 1107 60-61 0.5133141271527322 1107 62-63 0.36299048401199485 1107 64-65 0.4506183313932368 1107 66-67 0.38538657203530136 1107 68-69 0.33892158972317077 1107 70-71 0.1914373936056819 1107 72-73 0.12598622866793363 1107 74-75 0.24839336919747623 1107 76-77 0.24978477159889323 1107 78-79 0.33809989309711597 1107 80-81 0.11018131870351411 1107 82-83 0.17132693898756912 1107 84-85 0.3240505889115468 1107 86-87 0.03547867250981085 1107 88-89 0.13803929508883073 1107 90-91 0.17886410744215553 1107 92-93 0.1684871728368016 1107 94-95 0.1434886046302566 1107 96-97 0.16124072005283097 1107 98-99 0.19085573543511103 1107 100-101 0.20553251065875244 1108 1 0.19008133198892452 1108 2 0.21274199056017906 1108 3 0.1646152411308499 1108 4 0.14663401516276053 1108 5 0.3529783031404108 1108 6 0.10694502160455244 1108 7 0.11518147907177223 1108 8 0.08202282979689102 1108 9 0.08677246206607236 1108 10-11 0.22105334498933615 1108 12-13 0.3568439790172704 1108 14-15 0.47768552277710086 1108 16-17 0.4917113127817103 1108 18-19 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72-73 -2.7179544525962207 2316 74-75 -2.974341642343557 2316 76-77 -3.1267376534498155 2316 78-79 -3.438636935444844 2316 80-81 -3.249078943460024 2316 82-83 -3.0084747214294794 2316 84-85 -3.2705148908179 2316 86-87 -3.68199436190136 2316 88-89 -3.648104666588015 2316 90-91 -3.342852258582152 2316 92-93 -3.316759015091325 2316 94-95 -3.8208329665980756 2316 96-97 -3.816194260681595 2316 98-99 -3.748513795329959 2316 100-101 -3.5208535085067503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6021.0 3 2275.0 4 2378.0 5 2504.0 6 2812.0 7 3268.0 8 4059.0 9 4800.0 10 5926.0 11 6914.0 12 8312.0 13 9689.0 14 10616.0 15 12175.0 16 14671.0 17 18011.0 18 16509.0 19 12847.0 20 13111.0 21 14164.0 22 15372.0 23 17061.0 24 19263.0 25 21936.0 26 25149.0 27 29699.0 28 35189.0 29 42347.0 30 51242.0 31 63138.0 32 78982.0 33 102466.0 34 137731.0 35 194182.0 36 287756.0 37 407231.0 38 341798.0 39 57209.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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18-19 22.263864028010495 27.01256325123881 28.748656320833643 21.974916399917056 20-21 23.085922248405225 26.077704875361658 28.75653893038283 22.07983394585029 22-23 22.745705478709397 25.99918738613576 28.706287748714054 22.548819386440787 24-25 22.582642801676574 26.203493208180255 28.764677247683817 22.44918674245935 26-27 22.437178715876126 26.302559551110612 28.718413638231844 22.54184809478142 28-29 22.33067217420021 26.513146075286677 28.698559276116427 22.457622474396686 30-31 22.574667138739326 26.185570301737343 28.659281874554154 22.58048068496917 32-33 22.372814242346127 26.264114477783977 28.66823942900246 22.69483185086744 34-35 22.697264733877105 26.336392107623745 28.477740597038753 22.488602561460397 36-37 22.668547189162854 26.184687480270597 28.564652291946352 22.5821130386202 38-39 22.517078860808894 26.387143716880445 28.583725609881032 22.512051812429625 40-41 22.680859640796626 26.308082742952116 28.492636197468464 22.518421418782793 42-43 22.44230012819679 26.322367397562285 28.360579618163683 22.874752856077247 44-45 22.58435695177898 26.374133465027356 28.26394740434376 22.777562178849912 46-47 22.79447132135534 26.569523846280113 27.944713213553396 22.691291618811153 48-49 22.82029823040494 26.636525827568768 27.759803336504174 22.78337260552212 50-51 22.634567135917138 26.778391305290437 27.786800552627422 22.800241006165002 52-53 22.823393026080904 26.880581230602758 27.516588090976423 22.779437652339915 54-55 22.693767509722296 26.740787137288613 27.53744004196973 23.028005311019363 56-57 22.703924256551527 26.99875830802968 27.481066639851903 22.81625079556689 58-59 22.8996963267111 26.781954192173302 27.464992754821342 22.85335672629426 60-61 22.795290112343057 26.707734581780162 27.809767851032742 22.68720745484404 62-63 22.65563833817401 26.73896313380525 27.896270493463298 22.70912803455744 64-65 22.59590493955141 26.804493373325045 27.708690763825338 22.890910923298204 66-67 22.667048852061846 26.923436161219332 27.426996394495383 22.982518592223432 68-69 22.471271543959112 26.827173649886475 27.728178669264363 22.973376136890046 70-71 22.64837536593165 26.69541381550135 27.580726559287005 23.07548425928 72-73 22.678325878938338 26.727264452674422 27.532868079963723 23.061541588423516 74-75 22.60709007876012 27.041427337153074 27.30610556534345 23.045377018743356 76-77 22.701946209952 26.90360265213761 27.352838008380388 23.041613129530003 78-79 22.70233246830871 26.927352946012977 27.281259153003546 23.089055432674765 80-81 22.60734912689917 26.99289470648961 27.29325397468441 23.106502191926808 82-83 22.683656505998602 27.003664169871065 27.367650451158067 22.945028872972266 84-85 22.683335628786605 27.007183259616717 27.17648286678444 23.132998244812235 86-87 22.61216664077222 26.959931600534155 27.3626220176508 23.065279741042826 88-89 22.745233625218415 26.854539341579887 27.18975167171304 23.21047536148866 90-91 22.63870991723249 26.8428256087472 27.298361424470357 23.220103049549945 92-93 22.5894832967849 26.853901738167675 27.3324233162022 23.22419164884523 94-95 22.63483606977308 26.751064484313975 27.32774107242639 23.286358373486554 96-97 22.593209408429303 26.805787060097018 27.351404271991637 23.24959925948205 98-99 22.441874960819334 26.82999035351151 27.12076750484887 23.607367180820294 100-101 22.529080993869606 26.748578490315232 27.192648294236054 23.529692221579104 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 235.0 1 183.5 2 175.0 3 243.0 4 244.0 5 242.0 6 406.5 7 919.0 8 1661.5 9 2010.0 10 1811.0 11 1613.0 12 1663.0 13 1772.5 14 1882.5 15 1975.5 16 2071.5 17 2241.5 18 2395.0 19 2566.0 20 2873.5 21 3419.0 22 4206.0 23 5227.5 24 6517.5 25 8057.5 26 10290.0 27 13197.5 28 15711.5 29 18668.5 30 22477.5 31 26326.5 32 30069.0 33 33603.0 34 37562.5 35 41459.0 36 46166.5 37 51013.5 38 54337.5 39 56781.5 40 59152.0 41 62110.5 42 64563.0 43 67887.5 44 72300.5 45 75983.5 46 78730.5 47 80602.0 48 83403.5 49 83822.0 50 81144.5 51 79212.0 52 78151.0 53 77300.0 54 75018.0 55 70398.0 56 64392.5 57 58699.5 58 53802.0 59 47462.0 60 39896.0 61 32565.5 62 25692.5 63 19925.5 64 14859.5 65 10852.5 66 7949.0 67 5918.0 68 4265.0 69 2930.5 70 2026.5 71 1322.5 72 835.0 73 541.0 74 352.5 75 196.0 76 112.5 77 65.0 78 51.5 79 38.5 80 27.5 81 22.0 82 18.5 83 17.0 84 11.5 85 7.0 86 5.0 87 4.0 88 5.0 89 4.5 90 5.0 91 5.0 92 3.5 93 2.5 94 1.0 95 0.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041261044237938055 2 0.04221395519031537 3 0.02687208885704049 4 0.0456920801664926 5 0.06546498242832204 6 0.0665131844759371 7 0.06608437454736729 8 0.06956249952354453 9 0.06799019645212194 10-11 0.07554201574971223 12-13 0.07547054742828392 14-15 0.0698245500354483 16-17 0.05736523933311479 18-19 0.04988488835695282 20-21 0.05731759378549594 22-23 0.03166046639273653 24-25 0.018510295249929486 26-27 0.015222752464227721 28-29 0.017771789261837062 30-31 0.013578981071376842 32-33 0.009005008499965696 34-35 0.010696425440435442 36-37 0.0059318706785488305 38-39 0.00881442630949023 40-41 0.008957362952346828 42-43 0.005026605273790375 44-45 0.001310252559518818 46-47 0.0031684289166545965 48-49 8.81442630949023E-4 50-51 0.006217743964262028 52-53 0.005360124107122438 54-55 0.008409439154729869 56-57 0.023131913368959495 58-59 0.009290881785678893 60-61 0.020868749857063357 62-63 0.014150727642803234 64-65 0.010982298726148637 66-67 0.017819434809455928 68-69 0.02687208885704049 70-71 0.020058775547542634 72-73 0.01932026955945021 74-75 0.02072581321420676 76-77 0.028587328571319667 78-79 0.03899788072604191 80-81 0.032303681285591225 82-83 0.03220839019035349 84-85 0.0229175084046746 86-87 0.027157962142753685 88-89 0.021940774678487845 90-91 0.02020171219039923 92-93 0.03349481997606288 94-95 0.02456127979752548 96-97 0.0400699055474664 98-99 0.05610263232121485 100-101 0.06737080433307668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2098832.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.5219551020054 #Duplication Level Percentage of deduplicated Percentage of total 1 80.60472335605769 50.39564894677005 2 11.270916215216882 14.093594351325093 3 3.4064359979271632 6.389311155607712 4 1.448918410064266 3.6235684712202834 5 0.8072744986924837 2.5236189981122688 6 0.510012685218669 1.9132194124016904 7 0.3714830321519158 1.6258091820151235 8 0.2580367465760205 1.2906369507274797 9 0.20174601774375905 1.135219991704532 >10 1.0447476694964284 11.645854737190083 >50 0.055935135954090644 2.3418241059930978 >100 0.018931361029152525 1.9829365329139286 >500 4.5756756633142455E-4 0.18490316175824925 >1k 2.287837831656565E-4 0.2870814690423428 >5k 1.5252252211043764E-4 0.5667725332181246 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6816 0.3247520525701914 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5071 0.24161057197527006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.15399040990417528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.7645547618866115E-5 0.0 5 0.0 0.0 0.0 4.7645547618866115E-5 0.0 6 4.7645547618866115E-5 0.0 0.0 4.7645547618866115E-5 0.0 7 4.7645547618866115E-5 0.0 0.0 4.7645547618866115E-5 0.0 8 4.7645547618866115E-5 0.0 0.0 4.7645547618866115E-5 0.0 9 4.7645547618866115E-5 0.0 0.0 4.7645547618866115E-5 0.0 10-11 4.7645547618866115E-5 0.0 0.0 4.7645547618866115E-5 0.0 12-13 4.7645547618866115E-5 0.0 0.0 4.7645547618866115E-5 0.0 14-15 4.7645547618866115E-5 2.3822773809433058E-5 0.0 9.529109523773223E-5 7.146832142829917E-5 16-17 4.7645547618866115E-5 4.7645547618866115E-5 0.0 1.1911386904716528E-4 9.529109523773223E-5 18-19 4.7645547618866115E-5 4.7645547618866115E-5 0.0 1.4293664285659833E-4 9.529109523773223E-5 20-21 4.7645547618866115E-5 4.7645547618866115E-5 0.0 1.4293664285659833E-4 1.1911386904716528E-4 22-23 4.7645547618866115E-5 4.7645547618866115E-5 0.0 1.9058219047546446E-4 1.4293664285659833E-4 24-25 4.7645547618866115E-5 4.7645547618866115E-5 0.0 2.8587328571319666E-4 1.4293664285659833E-4 26-27 4.7645547618866115E-5 4.7645547618866115E-5 0.0 4.28809928569795E-4 1.4293664285659833E-4 28-29 4.7645547618866115E-5 4.7645547618866115E-5 0.0 7.861515357112909E-4 1.4293664285659833E-4 30-31 4.7645547618866115E-5 4.7645547618866115E-5 0.0 0.0015961258452320147 1.4293664285659833E-4 32-33 4.7645547618866115E-5 4.7645547618866115E-5 0.0 0.00257285957141877 1.4293664285659833E-4 34-35 4.7645547618866115E-5 4.7645547618866115E-5 0.0 0.0038831121309375882 1.4293664285659833E-4 36-37 4.7645547618866115E-5 4.7645547618866115E-5 0.0 0.005550706297597902 1.4293664285659833E-4 38-39 4.7645547618866115E-5 4.7645547618866115E-5 0.0 0.008623844119014765 1.4293664285659833E-4 40-41 4.7645547618866115E-5 9.529109523773223E-5 0.0 0.011816095809478795 1.4293664285659833E-4 42-43 4.7645547618866115E-5 1.4293664285659833E-4 0.0 0.01519892969041829 1.4293664285659833E-4 44-45 7.146832142829917E-5 1.4293664285659833E-4 0.0 0.018176776416597422 1.4293664285659833E-4 46-47 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.0226316351189614 1.9058219047546446E-4 48-49 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.025395076880855637 2.8587328571319666E-4 50-51 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.027967936452274408 2.8587328571319666E-4 52-53 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.03506712304748546 3.096960595226297E-4 54-55 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.04602559899982467 3.335188333320628E-4 56-57 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.06336857833309192 3.811643809509289E-4 58-59 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.08752487097585705 4.28809928569795E-4 60-61 9.529109523773223E-5 1.4293664285659833E-4 0.0 0.11363463107099567 4.764554761886611E-4 62-63 9.529109523773223E-5 1.9058219047546446E-4 0.0 0.12726125768999139 4.764554761886611E-4 64-65 9.529109523773223E-5 1.9058219047546446E-4 0.0 0.14560479352325484 4.764554761886611E-4 66-67 1.1911386904716528E-4 1.9058219047546446E-4 0.0 0.18479325643977224 4.764554761886611E-4 68-69 1.667594166660314E-4 1.9058219047546446E-4 0.0 0.24275406511812286 5.241010238075273E-4 70-71 1.9058219047546446E-4 1.9058219047546446E-4 0.0 0.298928165760766 5.717465714263933E-4 72-73 1.9058219047546446E-4 1.9058219047546446E-4 0.0 0.3661083879033672 6.193921190452595E-4 74-75 2.144049642848975E-4 1.9058219047546446E-4 0.0 0.4312160287245478 6.193921190452595E-4 76-77 2.6205051190376363E-4 1.9058219047546446E-4 0.0 0.4846028648314872 6.193921190452595E-4 78-79 2.8587328571319666E-4 1.9058219047546446E-4 0.0 0.535416841367008 6.193921190452595E-4 80-81 2.8587328571319666E-4 1.9058219047546446E-4 0.0 0.5874934249144286 6.193921190452595E-4 82-83 2.8587328571319666E-4 1.9058219047546446E-4 0.0 0.6534110400451298 6.193921190452595E-4 84-85 2.8587328571319666E-4 1.9058219047546446E-4 0.0 0.7846030554136777 6.193921190452595E-4 86-87 2.8587328571319666E-4 1.9058219047546446E-4 0.0 0.9674666671748858 6.193921190452595E-4 88-89 2.8587328571319666E-4 1.9058219047546446E-4 0.0 1.178607911447891 6.193921190452595E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5315 0.0 34.065243 1 GTATCAA 8280 0.0 33.46016 1 ATCAACG 9955 0.0 27.781187 3 TCAACGC 9985 0.0 27.6997 4 TATCAAC 10065 0.0 27.478876 2 CAACGCA 10145 0.0 27.26674 5 AACGCAG 10325 0.0 26.928202 6 GTACATG 7155 0.0 25.50418 1 TACATGG 6965 0.0 25.244709 2 ACATGGG 7135 0.0 23.643047 3 ACGCAGA 11730 0.0 23.21547 7 CGCAGAG 11715 0.0 23.001791 8 GAGTACT 6020 0.0 21.707531 12-13 GCAGAGT 12585 0.0 20.505363 9 GTACTTT 6650 0.0 19.615067 14-15 AGAGTAC 9670 0.0 19.535349 10-11 GTATAGG 675 0.0 19.008587 1 CATGGGG 4470 0.0 18.923986 4 AGTACTT 6615 0.0 18.785212 12-13 CATGGGA 3260 0.0 18.221855 4 >>END_MODULE