FastQCFastQC Report
Thu 26 May 2016
SRR936998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098832
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72290.34442966373678313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57270.27286605121324625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38840.18505530695167596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86850.033.8690341
GGTATCA57500.032.396661
ATCAACG107200.027.0296923
TCAACGC107650.026.8693754
TATCAAC109550.026.713852
CAACGCA109600.026.3479795
AACGCAG113000.025.4693246
ACGCAGA130350.021.8242347
CGCAGAG132000.021.4075158
GAGTACT70750.021.21261612-13
GTATAGG6300.020.3659761
GTACATG73100.020.2173861
AGTACTT75150.020.03382512-13
AGAGTAC111150.019.61220710-11
TACATGG72900.019.5506782
ACATGGG73500.019.323683
GCAGAGT142350.019.217129
GTACTTT78100.018.91291414-15
GTATTAG5650.017.6625251
TACTTTT87850.016.97606514-15
Produced by FastQC (version 0.11.5)