FastQCFastQC Report
Thu 26 May 2016
SRR936997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176518
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71640.329149586633329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.24447305283025458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33080.15198587836167676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84750.038.238531
GGTATCA52350.033.3123931
ATCAACG102450.031.2639873
TCAACGC103450.030.9158344
CAACGCA104400.030.680035
TATCAAC105400.030.4791242
AACGCAG107200.029.9652826
ACGCAGA121350.026.2350277
CGCAGAG122450.025.8053258
GTACATG74950.023.9649561
TACATGG73650.023.9385342
GCAGAGT130000.023.5739789
TAGATCG1252.2496782E-422.810655
GAGTACT66100.021.88605312-13
ACATGGG76950.021.7385443
AGAGTAC103550.021.4969110-11
GTACCGT1604.9766353E-520.7895226
GTACTTT69850.020.50513314-15
AGTACTT70600.020.18822712-13
GTGTAGC14650.019.7855761
Produced by FastQC (version 0.11.5)