##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936997_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2176518 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.496833933833766 31.0 31.0 34.0 30.0 34.0 2 31.63454517720506 33.0 31.0 34.0 30.0 34.0 3 31.638326446186063 33.0 31.0 34.0 28.0 34.0 4 35.21409884963047 37.0 35.0 37.0 33.0 37.0 5 35.19612013316683 37.0 35.0 37.0 33.0 37.0 6 35.275807045933 37.0 35.0 37.0 33.0 37.0 7 35.19942679086504 37.0 35.0 37.0 32.0 37.0 8 35.156741639628066 37.0 35.0 37.0 32.0 37.0 9 36.72484766953455 39.0 37.0 39.0 32.0 39.0 10-11 36.587044076823624 39.0 37.0 39.0 32.0 39.0 12-13 36.46857480618125 39.0 37.0 39.0 32.0 39.0 14-15 37.58817317384924 40.0 37.0 41.0 32.0 41.0 16-17 37.490185470554344 40.0 37.0 41.0 32.0 41.0 18-19 37.40602880380497 40.0 37.0 41.0 32.0 41.0 20-21 37.27507537268242 40.0 37.0 41.0 31.0 41.0 22-23 37.13321828719083 39.5 36.5 41.0 31.0 41.0 24-25 37.25535488335038 40.0 37.0 41.0 31.0 41.0 26-27 37.199654677792694 40.0 37.0 41.0 31.0 41.0 28-29 37.099002167682514 40.0 37.0 41.0 31.0 41.0 30-31 36.917593376209155 39.0 36.0 41.0 30.0 41.0 32-33 36.70763944980009 39.0 36.0 41.0 30.0 41.0 34-35 36.50727331453266 39.0 35.5 41.0 30.0 41.0 36-37 36.31062251725003 39.0 35.0 41.0 30.0 41.0 38-39 36.1443541932573 39.0 35.0 40.5 29.5 41.0 40-41 35.94779092109507 39.0 35.0 40.0 28.5 41.0 42-43 35.71830373100521 38.5 35.0 40.0 27.5 41.0 44-45 35.46279676988658 38.0 35.0 40.0 27.0 41.0 46-47 35.13822352950906 38.0 34.0 40.0 25.5 41.0 48-49 34.899783966868185 38.0 34.0 40.0 24.0 41.0 50-51 34.20023794887063 37.5 33.5 39.5 23.5 40.5 52-53 34.39926984293261 38.0 33.5 39.5 24.0 40.5 54-55 34.79124661500617 38.0 34.0 40.0 24.5 41.0 56-57 34.66955913068489 38.0 34.0 40.0 24.0 41.0 58-59 34.38875304500124 38.0 34.0 40.0 23.5 41.0 60-61 34.07859709866861 38.0 33.5 40.0 23.0 41.0 62-63 33.71419096924537 37.0 33.0 40.0 22.0 41.0 64-65 33.350543390865596 36.5 33.0 39.5 21.5 41.0 66-67 32.949107243771934 36.0 32.0 39.0 20.0 41.0 68-69 32.51760817048148 35.5 32.0 39.0 18.5 40.5 70-71 32.10483074341678 35.0 31.0 38.5 18.0 40.0 72-73 31.679560426332333 35.0 31.0 37.5 15.0 40.0 74-75 31.213180639902816 35.0 31.0 37.0 12.0 39.0 76-77 30.753618394150656 35.0 30.0 36.5 7.5 39.0 78-79 30.31273070105554 34.5 30.0 36.0 7.0 38.5 80-81 29.85493274119488 34.0 29.5 35.5 2.0 37.0 82-83 29.41618883923772 34.0 29.0 35.0 2.0 37.0 84-85 29.00189614788391 34.0 29.0 35.0 2.0 36.5 86-87 28.62223698586458 34.0 29.0 35.0 2.0 36.0 88-89 28.239448513635082 34.0 27.5 35.0 2.0 36.0 90-91 27.816266394305032 34.0 27.0 35.0 2.0 35.0 92-93 27.398910094012546 33.5 26.0 35.0 2.0 35.0 94-95 26.925327748265808 33.0 25.5 35.0 2.0 35.0 96-97 26.341453413204025 33.0 25.0 35.0 2.0 35.0 98-99 25.599520656387863 33.0 22.0 35.0 2.0 35.0 100-101 22.968822219710567 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4241778252823174 1101 2 0.38469321330379813 1101 3 0.35554920117795774 1101 4 0.2606777387970425 1101 5 0.3137872860832047 1101 6 0.24344146387213073 1101 7 0.34597245787668385 1101 8 0.20980027830773906 1101 9 0.4435670638133331 1101 10-11 0.4815716459038697 1101 12-13 0.5039560437780963 1101 14-15 0.4879107489661507 1101 16-17 0.6105316414504003 1101 18-19 0.7032810279052129 1101 20-21 0.7273435911524402 1101 22-23 0.7025440763490209 1101 24-25 0.6293093101813128 1101 26-27 0.5871754648509295 1101 28-29 0.5804189649032736 1101 30-31 0.5353551809153245 1101 32-33 0.6917352360032893 1101 34-35 0.6036851700743853 1101 36-37 0.41347779916365823 1101 38-39 0.7587279108835645 1101 40-41 0.5320175529679219 1101 42-43 0.6488676320060662 1101 44-45 0.5727272776608885 1101 46-47 0.5889291934785277 1101 48-49 0.76463377621306 1101 50-51 0.668948163874397 1101 52-53 0.7382188698647099 1101 54-55 0.6776512673671036 1101 56-57 0.5281702971947126 1101 58-59 0.47099378091848365 1101 60-61 0.6131717075961589 1101 62-63 0.6462653330401125 1101 64-65 0.6848561799376256 1101 66-67 0.6309084265031188 1101 68-69 0.7511698479804565 1101 70-71 0.5831020027592118 1101 72-73 0.4004769801589596 1101 74-75 0.4934523298735094 1101 76-77 0.6007025403129838 1101 78-79 0.5292412053549747 1101 80-81 0.4900727880026068 1101 82-83 0.6189652641503116 1101 84-85 0.6354250731412705 1101 86-87 0.6184045636986788 1101 88-89 0.6273465488495802 1101 90-91 0.8404422373422378 1101 92-93 0.8462908729916379 1101 94-95 0.8831914047028455 1101 96-97 0.8502133265131135 1101 98-99 0.6365028706039659 1101 100-101 0.8233062130848801 1102 1 0.28010889115646975 1102 2 0.4221921750391786 1102 3 0.31527117883118194 1102 4 0.32247823915753315 1102 5 0.2981563368269349 1102 6 0.22409668360214852 1102 7 0.2875073618413566 1102 8 0.2969669953648477 1102 9 0.33750226945617356 1102 10-11 0.37460937560953056 1102 12-13 0.35273936376626835 1102 14-15 0.41435343842037753 1102 16-17 0.5123000968116642 1102 18-19 0.5510765014037204 1102 20-21 0.5414568467183685 1102 22-23 0.5095299360155181 1102 24-25 0.022164720619777256 1102 26-27 0.33698052028595527 1102 28-29 0.2596909669764784 1102 30-31 0.16806209459483767 1102 32-33 0.4974074002118982 1102 34-35 0.5675448398896208 1102 36-37 0.38208865093934463 1102 38-39 0.4888153244584217 1102 40-41 0.3129444657835947 1102 42-43 0.4044765866646358 1102 44-45 0.20244257719608072 1102 46-47 0.37750005960717914 1102 48-49 0.24698817828892317 1102 50-51 0.1677435277321493 1102 52-53 0.25969209260487247 1102 54-55 0.34739798063662164 1102 56-57 0.38418027117995024 1102 58-59 0.2873086558449671 1102 60-61 0.22255961322537843 1102 62-63 0.46726577160308835 1102 64-65 0.5066501849146547 1102 66-67 0.15857236433350153 1102 68-69 0.18878659045429202 1102 70-71 0.20817019597942732 1102 72-73 0.1380956330396863 1102 74-75 0.3472162467724722 1102 76-77 0.5551029549543429 1102 78-79 0.26471787679489367 1102 80-81 0.16484727692722956 1102 82-83 0.3433944677930434 1102 84-85 0.1530206682413251 1102 86-87 0.07702556554928108 1102 88-89 0.11211113688279184 1102 90-91 0.04133448042573562 1102 92-93 0.16559287675929113 1102 94-95 0.29226176748080945 1102 96-97 0.27187900198315873 1102 98-99 0.3296269008648096 1102 100-101 0.3283947064549402 1103 1 0.030580237120151565 1103 2 0.20491805764636695 1103 3 0.137868116637744 1103 4 0.21551806862819234 1103 5 0.2151185702111249 1103 6 0.07185897723480394 1103 7 0.05321452813365113 1103 8 0.10567306146788269 1103 9 0.11090273834636122 1103 10-11 0.0916566608270557 1103 12-13 0.3323951944419079 1103 14-15 0.36793552039757316 1103 16-17 0.36559542559143665 1103 18-19 0.2957467229477757 1103 20-21 0.2571974934724892 1103 22-23 0.4142817876733318 1103 24-25 0.12389146111264182 1103 26-27 0.33466619359523264 1103 28-29 0.3104644556897256 1103 30-31 0.3812336684548612 1103 32-33 0.3125559585055555 1103 34-35 0.09777917037861528 1103 36-37 0.3082831421345489 1103 38-39 0.485767783839826 1103 40-41 0.3821408855843842 1103 42-43 0.29164890380729247 1103 44-45 0.39081920865231723 1103 46-47 0.4469668607414192 1103 48-49 0.35452954998917363 1103 50-51 0.43141256067888634 1103 52-53 0.6005987500596319 1103 54-55 0.5764608223992838 1103 56-57 0.6109893621485725 1103 58-59 0.475871680972503 1103 60-61 0.6636410403280166 1103 62-63 0.808111362005242 1103 64-65 0.5570633289019185 1103 66-67 0.4034082691409324 1103 68-69 0.5340031410990136 1103 70-71 0.630332297078958 1103 72-73 0.36701456611805483 1103 74-75 0.4112115709681241 1103 76-77 0.4498328519425776 1103 78-79 0.28593040978419637 1103 80-81 0.4092273071824337 1103 82-83 0.47193324619004073 1103 84-85 0.5567941373083265 1103 86-87 0.46505838786216813 1103 88-89 0.481512450561155 1103 90-91 0.5348897780334774 1103 92-93 0.5780581684076758 1103 94-95 0.7122560700721472 1103 96-97 0.33344113975566714 1103 98-99 0.47469978840320337 1103 100-101 0.9830357205936799 1104 1 0.40639988233627733 1104 2 0.37796833763919224 1104 3 0.37231285436390493 1104 4 0.32976669771158384 1104 5 0.42688572024535176 1104 6 0.3770896776841539 1104 7 0.3840727181448429 1104 8 0.44329900772239483 1104 9 0.4513475569004015 1104 10-11 0.5136241313943444 1104 12-13 0.49599934747939045 1104 14-15 0.624750163125718 1104 16-17 0.6358794345532246 1104 18-19 0.5465333739708811 1104 20-21 0.6339180853851545 1104 22-23 0.5125894855185109 1104 24-25 0.4996605889040424 1104 26-27 0.4935662271893193 1104 28-29 0.3930138743604559 1104 30-31 0.3017566564089833 1104 32-33 0.6003455180693891 1104 34-35 0.5622340052750445 1104 36-37 0.48101200501258035 1104 38-39 0.46097619979019555 1104 40-41 0.40790177272088357 1104 42-43 0.611865598300632 1104 44-45 0.261834331043417 1104 46-47 0.4722754590596949 1104 48-49 0.3724401871907119 1104 50-51 0.2869772444524443 1104 52-53 0.23257283840955978 1104 54-55 0.1433424196592199 1104 56-57 0.3032975159135134 1104 58-59 0.5325915403825192 1104 60-61 0.48760833787312663 1104 62-63 0.5684663290047354 1104 64-65 0.5541154793023964 1104 66-67 0.35138589876233084 1104 68-69 0.4110697773710257 1104 70-71 0.38402985683475777 1104 72-73 0.3921968708745247 1104 74-75 0.3446043464712112 1104 76-77 0.29525395256570874 1104 78-79 0.38633094981546634 1104 80-81 0.4158005713405757 1104 82-83 0.42520083951466603 1104 84-85 0.4313841568333139 1104 86-87 0.5573897164956954 1104 88-89 0.43555864369432484 1104 90-91 0.3476303672687173 1104 92-93 0.027467019818249838 1104 94-95 0.46746315362544166 1104 96-97 0.48975089239709746 1104 98-99 0.2812701478094226 1104 100-101 0.34671176624122424 1105 1 0.1573066838708499 1105 2 0.28845413979953705 1105 3 0.22077032488056858 1105 4 0.32601617544081307 1105 5 0.33158073630815466 1105 6 0.270509351330702 1105 7 0.18524780359436477 1105 8 0.3317873041021002 1105 9 0.40992221261380024 1105 10-11 0.28350846149609055 1105 12-13 0.40560244071716767 1105 14-15 0.3344720709816187 1105 16-17 0.519985570117278 1105 18-19 0.5659101741803596 1105 20-21 0.33419711587308853 1105 22-23 0.3870218033482473 1105 24-25 0.12789161422854534 1105 26-27 0.3092035370082584 1105 28-29 0.19306786083181748 1105 30-31 0.09550883980194413 1105 32-33 0.24689453670755768 1105 34-35 -0.0615471780145711 1105 36-37 0.21216365722685282 1105 38-39 0.33082632472307694 1105 40-41 0.23016993997686797 1105 42-43 0.1799437116208722 1105 44-45 -0.04729860755149673 1105 46-47 -0.024606132726503915 1105 48-49 -0.06719545643390745 1105 50-51 -0.09287480154922889 1105 52-53 -0.017009027969017154 1105 54-55 0.18934520172188485 1105 56-57 0.2705857719697917 1105 58-59 0.31251630803146213 1105 60-61 0.027246232254917402 1105 62-63 0.13186635449218187 1105 64-65 0.05391877580573379 1105 66-67 0.119513370236362 1105 68-69 0.34534212025707944 1105 70-71 0.22153390661262762 1105 72-73 0.03576062468100005 1105 74-75 0.11697275167344046 1105 76-77 0.13552611665921788 1105 78-79 0.18204937565491974 1105 80-81 0.16943189858355012 1105 82-83 0.03473282784623777 1105 84-85 0.07867592533881762 1105 86-87 0.08185338642184092 1105 88-89 0.1960207019515252 1105 90-91 0.10691410659426737 1105 92-93 0.055000437222116005 1105 94-95 0.0920912239276852 1105 96-97 0.08076485533378275 1105 98-99 0.2871091191309709 1105 100-101 0.43089277910731383 1106 1 0.05601415497741158 1106 2 0.26075937431689766 1106 3 0.24789679035405499 1106 4 0.35120060252284446 1106 5 0.30156700724497654 1106 6 0.31413132781656117 1106 7 0.38588433288719415 1106 8 0.3512647676368772 1106 9 0.335732684976783 1106 10-11 0.38232684679184814 1106 12-13 0.39122719629823877 1106 14-15 0.5346112753616055 1106 16-17 0.48575884920001755 1106 18-19 0.3224393838023545 1106 20-21 0.5008147739445334 1106 22-23 0.4595120889959716 1106 24-25 0.3321380550729245 1106 26-27 0.38926094945428247 1106 28-29 0.4882478779723485 1106 30-31 0.31508332204985123 1106 32-33 0.4198480326262839 1106 34-35 0.5028689207681225 1106 36-37 0.5137018115415231 1106 38-39 0.42706547668299777 1106 40-41 0.3460051246535656 1106 42-43 0.2471439812097529 1106 44-45 0.4939398371536612 1106 46-47 0.3248164977620078 1106 48-49 0.22953253338162227 1106 50-51 0.04569720946729916 1106 52-53 0.18339377442400462 1106 54-55 0.2823714200722378 1106 56-57 0.34444230741989657 1106 58-59 0.18914752875318896 1106 60-61 0.2901095323888825 1106 62-63 0.38708814126734836 1106 64-65 0.3320567820129341 1106 66-67 0.25864669192079504 1106 68-69 0.14471956964676025 1106 70-71 0.052016753604299026 1106 72-73 0.08782372844775566 1106 74-75 0.29946728084978247 1106 76-77 0.24256762835388557 1106 78-79 0.2860011824828419 1106 80-81 0.37702440334711795 1106 82-83 0.3186505200323211 1106 84-85 0.40355922830179836 1106 86-87 0.5073814561155565 1106 88-89 0.4869863127284262 1106 90-91 0.3951993050000304 1106 92-93 0.3044443685601621 1106 94-95 0.09374411992795117 1106 96-97 0.22538730609636914 1106 98-99 0.14306969591803664 1106 100-101 0.34106693019357337 1107 1 0.2931888102190783 1107 2 0.3458294974926943 1107 3 0.3666976673121489 1107 4 0.3409865511103405 1107 5 0.48566394123881196 1107 6 0.38753004250393985 1107 7 0.3410506871514869 1107 8 0.34006698346228603 1107 9 0.40803285902953235 1107 10-11 0.44327233815615585 1107 12-13 0.423823008990702 1107 14-15 0.48249971424231575 1107 16-17 0.5828799497489499 1107 18-19 0.42359415686434687 1107 20-21 0.650270608058868 1107 22-23 0.5641922670653159 1107 24-25 0.6020528696397989 1107 26-27 0.31184496337849765 1107 28-29 0.5426555965424313 1107 30-31 0.6137569229335043 1107 32-33 0.5402338774515627 1107 34-35 0.6319327177958343 1107 36-37 0.6767726710943478 1107 38-39 0.43870291141321616 1107 40-41 0.47913698634857127 1107 42-43 0.5494682548742347 1107 44-45 0.6022744535681781 1107 46-47 0.6430143823516445 1107 48-49 0.5837166611095626 1107 50-51 0.38249137535296285 1107 52-53 0.6423856910125707 1107 54-55 0.9073236392448152 1107 56-57 0.8102392681910899 1107 58-59 0.6884093944648058 1107 60-61 0.7837030522710577 1107 62-63 0.731786561403915 1107 64-65 0.7197657289937851 1107 66-67 0.7121421397719061 1107 68-69 0.6149670927422264 1107 70-71 0.5013932598300315 1107 72-73 0.6449636568183585 1107 74-75 0.6945325506315463 1107 76-77 0.5944980239891784 1107 78-79 0.4419363813046395 1107 80-81 0.41165780473953717 1107 82-83 0.5012277559407394 1107 84-85 0.4056315438728255 1107 86-87 0.3232171603597678 1107 88-89 0.4611080157676568 1107 90-91 0.30605174508571764 1107 92-93 0.16570876138383284 1107 94-95 0.18942961999489327 1107 96-97 0.2750335104075212 1107 98-99 0.20812542627015773 1107 100-101 -0.06570798947438661 1108 1 -0.027464614097329587 1108 2 0.19792408641773207 1108 3 0.1912828231942001 1108 4 0.26760116461272077 1108 5 0.36267167714608917 1108 6 0.27109827710385304 1108 7 0.18926632624353346 1108 8 0.28348412020216784 1108 9 0.20216380103458675 1108 10-11 0.07083047588128721 1108 12-13 0.22315269790328784 1108 14-15 0.32366307099810143 1108 16-17 0.36451210979398496 1108 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-2.6423871086803743 2316 82-83 -1.99725922856598 2316 84-85 -1.759098462962445 2316 86-87 -2.2565668166281547 2316 88-89 -2.2638186474813757 2316 90-91 -2.1322563831895174 2316 92-93 -2.670187798196938 2316 94-95 -2.1750397921718836 2316 96-97 -2.54120452786999 2316 98-99 -2.384246839621845 2316 100-101 -2.346765747505831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6200.0 3 2506.0 4 2567.0 5 2682.0 6 3131.0 7 3601.0 8 4385.0 9 5446.0 10 6674.0 11 7826.0 12 9347.0 13 10696.0 14 11650.0 15 13392.0 16 15863.0 17 19590.0 18 18442.0 19 14180.0 20 14422.0 21 15577.0 22 17135.0 23 19268.0 24 21482.0 25 24656.0 26 28444.0 27 33183.0 28 39425.0 29 46318.0 30 56500.0 31 68835.0 32 86501.0 33 110015.0 34 147458.0 35 205669.0 36 298530.0 37 408881.0 38 325644.0 39 50383.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.407704927826614 18.71259782813498 14.67778181166447 23.20191543237393 2 15.367709361462015 19.791442660976234 40.667899961585896 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28.702237741383108 22.074183641514736 22-23 22.753915560858015 26.004337081124646 28.70546697828955 22.53628037972779 24-25 22.57872581298777 26.197209436530027 28.78324522462437 22.44081952585783 26-27 22.44695942026655 26.29080629951287 28.67022663497996 22.59200764524062 28-29 22.346210085838635 26.490678128664914 28.70536775761641 22.45774402788004 30-31 22.5761514492414 26.192799691985087 28.630110111655405 22.600938747118104 32-33 22.4159497507593 26.28540041502212 28.6418328788214 22.656816955397183 34-35 22.740685521710716 26.362916907399093 28.464531068553317 22.431866502336874 36-37 22.674381741846382 26.1760297870268 28.56183592324989 22.58775254787693 38-39 22.531256432310045 26.403208436250292 28.560168747059517 22.505366384380146 40-41 22.723553273418034 26.317782034323645 28.432950443300008 22.525714248958312 42-43 22.476001795529278 26.323249485301513 28.333548508925038 22.867200210244164 44-45 22.630739242850105 26.36344527438902 28.251805923912855 22.754009558848022 46-47 22.812152900217892 26.523058050302833 27.938989693397826 22.725799356081446 48-49 22.813413590295283 26.654357964979695 27.737973975756592 22.794254468968425 50-51 22.63216655984452 26.812220453339396 27.766038828126614 22.789574158689465 52-53 22.8233002803576 26.924253827005302 27.454737087791166 22.797708804845932 54-55 22.771013038472177 26.742904375041928 27.510748997694446 22.975333588791454 56-57 22.752239708414063 26.984885442802724 27.448425484640325 22.814449364142888 58-59 22.923157893044117 26.754293771799663 27.491396145776548 22.83115218937967 60-61 22.784563528966356 26.680874779917445 27.83104735404713 22.70351433706907 62-63 22.634074038678897 26.695073338466997 27.90555777115752 22.76529485169659 64-65 22.61995367325424 26.80789976000421 27.662438235025878 22.909708331715674 66-67 22.649098522139195 26.965830571558215 27.431876532856986 22.953194373445605 68-69 22.504616347464555 26.822052872543317 27.68691870610392 22.98641207388821 70-71 22.62603683596886 26.71984139494506 27.624219256598547 23.029902512487528 72-73 22.71612721788535 26.746989668096088 27.492909567809896 23.04397354620866 74-75 22.591619752580367 27.03200298142446 27.327227417708688 23.049149848286486 76-77 22.73895134743869 26.897346485054953 27.302514491213355 23.061187676293006 78-79 22.697529333833 26.918471853605062 27.237503926150914 23.146494886411027 80-81 22.64438615558682 27.027871169929078 27.241471002799965 23.08627167168414 82-83 22.739955703384567 26.96268063004329 27.336108727554674 22.96125493901747 84-85 22.73574165235423 26.95620106725793 27.198626005939424 23.10943127444842 86-87 22.639605944468936 26.92175937275344 27.347608250279208 23.091026432498417 88-89 22.815715886431587 26.80127549303127 27.160378383706146 23.222630236830998 90-91 22.695805625893264 26.854196442111938 27.294566660998797 23.155431270995997 92-93 22.638071659249746 26.824375965413548 27.31794615606276 23.21960621927394 94-95 22.62640131270899 26.789386209295472 27.331370335294952 23.252842142700583 96-97 22.595494811161 26.81841430109558 27.308852986954264 23.27723790078916 98-99 22.476517690117838 26.860334112074906 27.102964158355913 23.560184039451336 100-101 22.665056767780207 26.70510170032164 27.07415432048264 23.555687211415513 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 223.0 1 168.0 2 164.0 3 230.0 4 231.5 5 239.0 6 419.0 7 935.5 8 1675.5 9 2081.5 10 1925.5 11 1691.5 12 1689.5 13 1851.0 14 2007.5 15 2087.5 16 2167.5 17 2290.0 18 2491.0 19 2718.0 20 3012.5 21 3539.5 22 4312.0 23 5318.0 24 6541.0 25 8270.5 26 10800.0 27 13664.0 28 16267.5 29 19279.5 30 23172.5 31 27379.0 32 31265.5 33 34832.0 34 39051.5 35 43189.5 36 48064.0 37 52893.0 38 56229.0 39 58824.5 40 61136.0 41 64062.5 42 66528.0 43 69918.0 44 74873.5 45 78706.5 46 81163.0 47 83248.0 48 86251.0 49 86964.0 50 84287.0 51 82019.0 52 80953.5 53 79987.5 54 77584.0 55 72946.0 56 67159.5 57 61304.0 58 55990.0 59 50023.5 60 41923.0 61 33863.5 62 26747.0 63 20721.0 64 15615.0 65 11379.5 66 8348.5 67 6174.5 68 4448.0 69 3121.0 70 2180.5 71 1383.5 72 856.5 73 567.5 74 355.5 75 205.5 76 126.5 77 77.0 78 52.5 79 37.0 80 26.0 81 18.0 82 17.5 83 22.0 84 18.5 85 11.0 86 6.0 87 7.5 88 6.0 89 2.5 90 3.0 91 3.5 92 2.5 93 1.5 94 1.0 95 0.5 96 1.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023340032106327627 2 0.010751117151339893 3 0.011807850888437403 4 0.019986051114670313 5 0.05279074190978435 6 0.039512652778428664 7 0.0153915566055507 8 0.04465848662864263 9 0.026280508592164183 10-11 0.021617096665407776 12-13 0.033562782389118764 14-15 0.018194198256113663 16-17 0.016632070123012998 18-19 0.016057758309373043 20-21 0.0130253919333541 22-23 0.007787668192957742 24-25 0.0018377978036478448 26-27 2.297247254559806E-5 28-29 0.002320219727105404 30-31 0.0 32-33 3.445870881839709E-4 34-35 2.297247254559806E-5 36-37 0.0 38-39 2.7566967054717674E-4 40-41 4.594494509119612E-5 42-43 4.594494509119612E-5 44-45 2.297247254559806E-5 46-47 2.297247254559806E-5 48-49 4.135045058207651E-4 50-51 1.3783483527358837E-4 52-53 5.513393410943535E-4 54-55 0.0016540180232830603 56-57 3.2161461563837287E-4 58-59 0.0010337612645519127 60-61 0.0028485865956541594 62-63 0.001562128133100668 64-65 9.878163194607166E-4 66-67 0.010590309843520706 68-69 0.013071336878445297 70-71 0.025131884964884277 72-73 0.015896951001553857 74-75 0.017321244299380936 76-77 0.028968287879999152 78-79 0.01858473028938883 80-81 0.0438544500895467 82-83 0.03358575486166437 84-85 0.017482051607200125 86-87 0.012703777317715726 88-89 0.012014603141347786 90-91 0.023340032106327627 92-93 0.029266930023091928 94-95 0.01171596099825501 96-97 0.01860770276193443 98-99 0.026257536119618584 100-101 0.03730729541405125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2176518.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.04981491053201 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34429277628455 49.8534849588605 2 11.340844957189415 14.073946610452873 3 3.4961859212344835 6.5081306791622255 4 1.5377990290641 3.8168058049209295 5 0.8209341759899729 2.5469406836953965 6 0.515708477782691 1.9199769356524912 7 0.34876555284416655 1.5148586600804934 8 0.2531393897385697 1.256580182387462 9 0.19006686043366872 1.0614252159481556 >10 1.0742322186759248 11.95418627975584 >50 0.05739525972267395 2.4112238585729995 >100 0.019968126214769365 2.0937355738875296 >500 2.96557699965207E-4 0.13497390513363602 >1k 2.2241827497388543E-4 0.2793785023437348 >5k 1.4827884998259029E-4 0.5743521491456494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7164 0.329149586633329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5321 0.24447305283025458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3308 0.15198587836167676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.594494509119612E-5 7 0.0 0.0 0.0 9.188989018239224E-5 4.594494509119612E-5 8 0.0 0.0 0.0 9.188989018239224E-5 9.188989018239224E-5 9 0.0 0.0 0.0 9.188989018239224E-5 9.188989018239224E-5 10-11 0.0 0.0 0.0 1.3783483527358837E-4 9.188989018239224E-5 12-13 0.0 0.0 0.0 1.8377978036478447E-4 9.188989018239224E-5 14-15 0.0 2.297247254559806E-5 0.0 1.8377978036478447E-4 9.188989018239224E-5 16-17 0.0 4.594494509119612E-5 0.0 1.8377978036478447E-4 9.188989018239224E-5 18-19 0.0 4.594494509119612E-5 0.0 1.8377978036478447E-4 9.188989018239224E-5 20-21 0.0 4.594494509119612E-5 0.0 2.297247254559806E-4 9.188989018239224E-5 22-23 0.0 4.594494509119612E-5 0.0 3.6755956072956895E-4 9.188989018239224E-5 24-25 0.0 4.594494509119612E-5 0.0 6.202567587311476E-4 9.188989018239224E-5 26-27 0.0 4.594494509119612E-5 0.0 7.81064066550334E-4 1.3783483527358837E-4 28-29 0.0 4.594494509119612E-5 0.0 0.0011256511547343049 1.3783483527358837E-4 30-31 0.0 4.594494509119612E-5 0.0 0.0019756326389214333 1.3783483527358837E-4 32-33 0.0 4.594494509119612E-5 0.0 0.0028715590681997576 1.3783483527358837E-4 34-35 0.0 4.594494509119612E-5 0.0 0.003997210222934063 1.3783483527358837E-4 36-37 0.0 6.891741763679417E-5 0.0 0.005835008026581908 1.3783483527358837E-4 38-39 0.0 9.188989018239224E-5 0.0 0.009074126655511234 1.3783483527358837E-4 40-41 0.0 9.188989018239224E-5 0.0 0.011922713251165394 1.3783483527358837E-4 42-43 0.0 9.188989018239224E-5 0.0 0.014863189737001944 1.3783483527358837E-4 44-45 0.0 9.188989018239224E-5 0.0 0.017137464519016155 1.3783483527358837E-4 46-47 0.0 9.188989018239224E-5 0.0 0.021134674741950216 1.3783483527358837E-4 48-49 0.0 9.188989018239224E-5 0.0 0.023500839414146817 1.8377978036478447E-4 50-51 0.0 9.188989018239224E-5 0.0 0.026372398482346574 1.8377978036478447E-4 52-53 0.0 9.188989018239224E-5 0.0 0.03280469079511403 1.8377978036478447E-4 54-55 0.0 9.188989018239224E-5 0.0 0.043877422562092296 2.0675225291038254E-4 56-57 0.0 1.3783483527358837E-4 0.0 0.06191081351038677 2.297247254559806E-4 58-59 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.08486031358343923 2.297247254559806E-4 60-61 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.11086515250505624 2.7566967054717674E-4 62-63 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.1257742871871494 3.2161461563837287E-4 64-65 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.14229149494743437 3.2161461563837287E-4 66-67 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.1801041847574888 3.2161461563837287E-4 68-69 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.23484758683364898 3.445870881839709E-4 70-71 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.289085594513806 3.90532033275167E-4 72-73 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.35329365527875256 4.135045058207651E-4 74-75 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.4156868907125969 4.135045058207651E-4 76-77 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.4686843848752916 4.135045058207651E-4 78-79 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.5202116407950681 4.135045058207651E-4 80-81 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.5754604372672314 4.8242192345755925E-4 82-83 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.6435508458923841 5.513393410943535E-4 84-85 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.7697156651128085 5.513393410943535E-4 86-87 4.594494509119612E-5 1.3783483527358837E-4 0.0 0.9419402917871573 5.513393410943535E-4 88-89 6.891741763679417E-5 1.3783483527358837E-4 0.0 1.147429058707532 5.513393410943535E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8475 0.0 38.23853 1 GGTATCA 5235 0.0 33.312393 1 ATCAACG 10245 0.0 31.263987 3 TCAACGC 10345 0.0 30.915834 4 CAACGCA 10440 0.0 30.68003 5 TATCAAC 10540 0.0 30.479124 2 AACGCAG 10720 0.0 29.965282 6 ACGCAGA 12135 0.0 26.235027 7 CGCAGAG 12245 0.0 25.805325 8 GTACATG 7495 0.0 23.964956 1 TACATGG 7365 0.0 23.938534 2 GCAGAGT 13000 0.0 23.573978 9 TAGATCG 125 2.2496782E-4 22.81065 5 GAGTACT 6610 0.0 21.886053 12-13 ACATGGG 7695 0.0 21.738544 3 AGAGTAC 10355 0.0 21.49691 10-11 GTACCGT 160 4.9766353E-5 20.789522 6 GTACTTT 6985 0.0 20.505133 14-15 AGTACTT 7060 0.0 20.188227 12-13 GTGTAGC 1465 0.0 19.785576 1 >>END_MODULE