FastQCFastQC Report
Thu 26 May 2016
SRR936997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176518
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73210.3363629430126468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61060.2805398347268435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37580.17266110365271503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57150.038.985791
GTATCAA87300.033.1944351
TATCAAC109650.026.336912
ATCAACG109400.026.1347983
TCAACGC110650.025.8395544
CAACGCA112300.025.4651645
AACGCAG114300.025.019586
GTACATG76600.023.256921
TACATGG76400.022.5674862
ACATGGG75750.021.8817833
ACGCAGA130750.021.5811467
GAGTACT74850.021.47864212-13
CGCAGAG133150.020.8710568
GTACTTT80100.019.7160114-15
AGAGTAC114200.019.50906810-11
GCAGAGT143700.019.0081929
AGTACTT79300.018.53648612-13
GTCTACG1559.61994E-418.3894711
CATGGGG48200.018.327614
AGTACAT57000.016.9990712
Produced by FastQC (version 0.11.5)