FastQCFastQC Report
Thu 26 May 2016
SRR936996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175305
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70200.3227133666313459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.24125352536770706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.15450706912364015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83700.036.343841
GGTATCA51500.035.9942931
TCAACGC100850.029.554794
TATCAAC101350.029.54972
ATCAACG101000.029.4167653
CAACGCA102250.029.1029665
AACGCAG104000.028.6556666
ACGCAGA117950.025.0610247
TACATGG78150.024.8180392
GTACATG80050.024.641271
CGCAGAG118850.024.5919118
ACATGGG78800.023.2861333
GCAGAGT128350.022.1417359
GAGTACT62600.021.67420412-13
AGAGTAC100800.020.41285510-11
GTACTTT67350.019.86356214-15
CTAGACG1200.004743141619.807294
AGTACTT66450.019.30990612-13
CATGGGG47600.018.5755754
CAGAGTA124150.018.14295410-11
Produced by FastQC (version 0.11.5)