##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175305 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.467916453095082 31.0 31.0 34.0 30.0 34.0 2 31.562350566931993 33.0 31.0 34.0 30.0 34.0 3 31.583087429119136 33.0 31.0 34.0 28.0 34.0 4 35.18480442972365 37.0 35.0 37.0 33.0 37.0 5 35.1707282427062 37.0 35.0 37.0 32.0 37.0 6 35.217571788783644 37.0 35.0 37.0 32.0 37.0 7 35.14974543799605 37.0 35.0 37.0 32.0 37.0 8 35.104312728559904 37.0 35.0 37.0 32.0 37.0 9 36.688118677610724 39.0 37.0 39.0 32.0 39.0 10-11 36.54182700816667 39.0 37.0 39.0 32.0 39.0 12-13 36.436143897062706 39.0 37.0 39.0 32.0 39.0 14-15 37.53302134643188 40.0 37.0 41.0 32.0 41.0 16-17 37.4418564293283 40.0 37.0 41.0 32.0 41.0 18-19 37.37371862796252 40.0 37.0 41.0 32.0 41.0 20-21 37.28289757068549 40.0 37.0 41.0 31.0 41.0 22-23 37.16128175129465 39.5 37.0 41.0 31.0 41.0 24-25 37.11861807884412 39.5 36.5 41.0 31.0 41.0 26-27 37.14147050643473 40.0 37.0 41.0 31.0 41.0 28-29 37.05992975697661 40.0 36.5 41.0 31.0 41.0 30-31 36.90328505657827 39.0 36.0 41.0 30.0 41.0 32-33 36.717857955551054 39.0 36.0 41.0 30.0 41.0 34-35 36.54336426386185 39.0 35.5 41.0 30.0 41.0 36-37 36.35876624197526 39.0 35.0 41.0 30.0 41.0 38-39 36.19664759654393 39.0 35.0 41.0 29.5 41.0 40-41 36.00216061655722 39.0 35.0 40.0 29.0 41.0 42-43 35.78652257959229 39.0 35.0 40.0 27.5 41.0 44-45 35.54653002682383 38.0 35.0 40.0 27.0 41.0 46-47 35.233013301583 38.0 34.5 40.0 26.0 41.0 48-49 35.008067604312956 38.0 34.0 40.0 25.0 41.0 50-51 34.325131418352825 37.5 33.5 39.5 24.0 40.5 52-53 34.517687864460385 38.0 33.5 39.5 24.5 40.5 54-55 34.895889771779125 38.0 34.0 40.0 25.0 41.0 56-57 34.75743907176235 38.0 34.0 40.0 24.5 41.0 58-59 34.52004523503601 38.0 34.0 40.0 24.0 41.0 60-61 34.20865878577946 38.0 34.0 40.0 23.0 41.0 62-63 33.87598612608347 37.0 33.0 40.0 22.5 41.0 64-65 33.52442944782456 37.0 33.0 40.0 22.0 41.0 66-67 33.131081388586885 36.0 32.5 39.0 21.5 41.0 68-69 32.72255499803475 36.0 32.0 39.0 20.0 41.0 70-71 32.29273688057536 35.0 32.0 38.5 18.5 40.0 72-73 31.887953183576556 35.0 31.0 37.5 18.0 40.0 74-75 31.478173865274066 35.0 31.0 37.0 15.5 39.0 76-77 31.011011789151407 35.0 31.0 36.5 12.0 39.0 78-79 30.54095816448728 35.0 30.0 36.0 7.5 38.5 80-81 30.09904748989222 34.0 30.0 36.0 7.0 37.5 82-83 29.68813384789719 34.0 29.5 35.0 2.0 37.0 84-85 29.303422968273416 34.0 29.0 35.0 2.0 36.5 86-87 28.937222366518718 34.0 29.0 35.0 2.0 36.0 88-89 28.547574018356045 34.0 29.0 35.0 2.0 36.0 90-91 28.159035859339266 34.0 28.0 35.0 2.0 35.0 92-93 27.74724808704986 34.0 27.0 35.0 2.0 35.0 94-95 27.33506014099172 34.0 26.5 35.0 2.0 35.0 96-97 26.791790346641044 33.0 25.0 35.0 2.0 35.0 98-99 26.1514272711183 33.0 24.5 35.0 2.0 35.0 100-101 23.889803958525356 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18693194024704596 1101 2 0.2176884804436483 1101 3 0.18090788167451421 1101 4 0.22046779402380423 1101 5 0.24012223721238968 1101 6 0.2384797434608643 1101 7 0.1456068023977224 1101 8 0.22408981815527795 1101 9 0.2319979214668635 1101 10-11 0.30520266272675656 1101 12-13 0.32978258048689923 1101 14-15 0.4089383938020106 1101 16-17 0.59731032212202 1101 18-19 0.48395628649280553 1101 20-21 0.5447987950951827 1101 22-23 0.6079263506890271 1101 24-25 0.5478772732055859 1101 26-27 0.45614743367851673 1101 28-29 0.4036543696371666 1101 30-31 0.3673978383408496 1101 32-33 0.33645054074263925 1101 34-35 0.4832681478536287 1101 36-37 0.5022439399188485 1101 38-39 0.5427077546580321 1101 40-41 0.5361766470878493 1101 42-43 0.35089906187879905 1101 44-45 0.4421107347608384 1101 46-47 0.60525013086054 1101 48-49 0.5930481273011523 1101 50-51 0.5032537633837322 1101 52-53 0.5597691138919814 1101 54-55 0.5798940963098929 1101 56-57 0.5533244928343137 1101 58-59 0.6018945877356217 1101 60-61 0.6211222034493318 1101 62-63 0.6863195307020575 1101 64-65 0.5748634497387712 1101 66-67 0.42985756375276907 1101 68-69 0.3749833837875869 1101 70-71 0.4844954559756971 1101 72-73 0.4867558512288106 1101 74-75 0.4390691499868602 1101 76-77 0.5193584303441376 1101 78-79 0.5970287753928396 1101 80-81 0.6691156960030007 1101 82-83 0.6293322693764694 1101 84-85 0.6834572542752362 1101 86-87 0.747878333235338 1101 88-89 0.626147646619529 1101 90-91 0.658525829925015 1101 92-93 0.7152916229767605 1101 94-95 0.8459727085148678 1101 96-97 0.5935411396617845 1101 98-99 0.5707927925425622 1101 100-101 0.9431193047798381 1102 1 0.2898619195597689 1102 2 0.2709725857189049 1102 3 0.30548407981266124 1102 4 0.24690512881290516 1102 5 0.3279102788168018 1102 6 0.39327876081519264 1102 7 0.176257015016958 1102 8 0.14725238567625354 1102 9 0.40998752036353636 1102 10-11 0.27661040321635255 1102 12-13 0.28099508479674284 1102 14-15 0.2952176289473911 1102 16-17 0.4781735131345428 1102 18-19 0.2584811255917643 1102 20-21 0.22993735675476046 1102 22-23 0.2508894440116336 1102 24-25 0.06771515103571346 1102 26-27 0.19408165100985997 1102 28-29 0.03888667052212469 1102 30-31 0.177839885232018 1102 32-33 0.31125049189767395 1102 34-35 0.08908393052229258 1102 36-37 0.15657066960509525 1102 38-39 0.27312128471205455 1102 40-41 0.10555265271938197 1102 42-43 0.1260847445589448 1102 44-45 0.43202389129456975 1102 46-47 0.4630358738785887 1102 48-49 0.4781331749968203 1102 50-51 0.39689924953474787 1102 52-53 0.5195007539333574 1102 54-55 0.2439987969190227 1102 56-57 0.49031449398360394 1102 58-59 0.22426019226384142 1102 60-61 0.3060937584430121 1102 62-63 0.21778861471538846 1102 64-65 0.5166607287871585 1102 66-67 0.23565215632833514 1102 68-69 0.4554339067159887 1102 70-71 0.4555260846390681 1102 72-73 0.3516151346445149 1102 74-75 0.37243349029257544 1102 76-77 0.3501465581455392 1102 78-79 0.36066406156684394 1102 80-81 0.4813894289072671 1102 82-83 0.45197119019016796 1102 84-85 0.27289381329143936 1102 86-87 0.3136394526468962 1102 88-89 0.5038215490445381 1102 90-91 0.26256559546920855 1102 92-93 0.497927957880794 1102 94-95 0.5805847502342054 1102 96-97 0.4775307672907907 1102 98-99 0.5525010731999558 1102 100-101 0.568034903561589 1103 1 0.26630874607955235 1103 2 0.2650791600783968 1103 3 0.254089997241536 1103 4 0.2939751550901306 1103 5 0.11221247953437796 1103 6 0.2521040551706406 1103 7 0.28565175451870317 1103 8 0.21067461519770347 1103 9 0.25429054224438374 1103 10-11 0.2355512920940157 1103 12-13 0.19076313775546083 1103 14-15 0.4853350868785711 1103 16-17 0.44271994675928283 1103 18-19 0.47677459039544345 1103 20-21 0.34359188073273117 1103 22-23 0.4397634712489875 1103 24-25 0.45912657278518054 1103 26-27 0.5265989270217872 1103 28-29 0.5016409153045771 1103 30-31 0.4653275677829285 1103 32-33 0.3296200722200737 1103 34-35 0.46246292263911926 1103 36-37 0.2976820509280529 1103 38-39 0.47162408298381564 1103 40-41 0.5341220283826331 1103 42-43 0.5809515581927869 1103 44-45 0.4661421198558955 1103 46-47 0.4964764848240577 1103 48-49 0.5375411794437426 1103 50-51 0.41271311708742786 1103 52-53 0.3938261367217706 1103 54-55 0.5026083779994153 1103 56-57 0.4634687716125896 1103 58-59 0.6224509484920517 1103 60-61 0.5871343618085234 1103 62-63 0.6349338721816551 1103 64-65 0.6205225649082422 1103 66-67 0.5847896190601531 1103 68-69 0.5751013987570204 1103 70-71 0.42008025614848776 1103 72-73 0.35477645532771973 1103 74-75 0.4777317304765134 1103 76-77 0.5124570589288027 1103 78-79 0.5842205512833551 1103 80-81 0.33807275044953755 1103 82-83 0.39830090619401304 1103 84-85 0.39470201481753975 1103 86-87 0.5009916432578443 1103 88-89 0.4207809393623947 1103 90-91 0.5360508650634266 1103 92-93 0.5120383853910262 1103 94-95 0.602515652503147 1103 96-97 0.5784785792065037 1103 98-99 0.6411844958084743 1103 100-101 0.8539694821153319 1104 1 0.1928155040166324 1104 2 0.12871074545348904 1104 3 0.10840764115398116 1104 4 0.21908827691232347 1104 5 0.20249443770519804 1104 6 0.13665996989217177 1104 7 0.17191543387263408 1104 8 0.1591703693612203 1104 9 0.28739291691375257 1104 10-11 0.2773013218527467 1104 12-13 0.1983776624892144 1104 14-15 0.09936976777996165 1104 16-17 0.2898311083700591 1104 18-19 0.16223841691981988 1104 20-21 0.3170633254920219 1104 22-23 0.1519435262523956 1104 24-25 0.1605692141855286 1104 26-27 0.26719833741244514 1104 28-29 0.33214817411587205 1104 30-31 0.43654932983984907 1104 32-33 0.17511815606703607 1104 34-35 0.07718166785646474 1104 36-37 0.16901728263648153 1104 38-39 0.13687817616187914 1104 40-41 0.13354695058481525 1104 42-43 0.20922096049834948 1104 44-45 0.12057848950724548 1104 46-47 0.104462645855115 1104 48-49 0.29392152847885455 1104 50-51 0.10938269006124557 1104 52-53 0.2599688178519557 1104 54-55 0.4041351940935627 1104 56-57 0.24538133814461105 1104 58-59 0.2944529303461749 1104 60-61 0.2941299822509933 1104 62-63 0.2571337302672987 1104 64-65 0.25363429232136525 1104 66-67 0.19915697937577193 1104 68-69 0.022884334714682097 1104 70-71 0.16537992269612545 1104 72-73 0.221127370273404 1104 74-75 0.1991352140257341 1104 76-77 0.22393241174366807 1104 78-79 0.23303994129895145 1104 80-81 0.2814840130081073 1104 82-83 0.23257654778450032 1104 84-85 0.3494824263073468 1104 86-87 0.36210888647092077 1104 88-89 0.1629565576096752 1104 90-91 0.13828215145990086 1104 92-93 0.16146864097600755 1104 94-95 0.2829809399106402 1104 96-97 0.05628243130020394 1104 98-99 -0.007805562456798754 1104 100-101 -0.19520469379973093 1105 1 0.04511975529944934 1105 2 0.07889720300106262 1105 3 0.1703311740591964 1105 4 -0.03069000649870901 1105 5 0.02960575512820185 1105 6 0.08901127550720389 1105 7 0.102901250669575 1105 8 0.07641784312383493 1105 9 -0.08555499577953896 1105 10-11 0.10669773906486313 1105 12-13 0.08343287047475201 1105 14-15 0.1639111735313037 1105 16-17 0.13823141961353969 1105 18-19 0.06923191460721512 1105 20-21 0.1383265408946457 1105 22-23 0.25623910482568135 1105 24-25 0.17071681068891564 1105 26-27 0.15578832771304718 1105 28-29 0.05893174416357283 1105 30-31 0.126383834299709 1105 32-33 -0.0020850029147752025 1105 34-35 -0.1606882072561362 1105 36-37 -0.09416506463273322 1105 38-39 0.10681247131874017 1105 40-41 0.11939783337671628 1105 42-43 0.14446449678995776 1105 44-45 0.17446362683835304 1105 46-47 0.20224132624744584 1105 48-49 0.09076286374382647 1105 50-51 -0.10604482105946289 1105 52-53 0.11156162602215858 1105 54-55 -0.10205249815462025 1105 56-57 -0.05989228248015621 1105 58-59 0.019761217077011395 1105 60-61 0.16575330280926437 1105 62-63 0.04206943166379773 1105 64-65 0.09481973807396571 1105 66-67 0.005901285690590896 1105 68-69 0.01739429269871806 1105 70-71 -0.08360273347020097 1105 72-73 0.06603348514875407 1105 74-75 0.15115660028314082 1105 76-77 0.1121132925284769 1105 78-79 0.10227170322685808 1105 80-81 -0.037766868705734424 1105 82-83 -0.009092710992444353 1105 84-85 -0.02661010457839552 1105 86-87 0.052461733717780845 1105 88-89 0.05319963255320914 1105 90-91 -0.07163232951084453 1105 92-93 0.009180497240876662 1105 94-95 0.11881458952899138 1105 96-97 0.01355113658630458 1105 98-99 0.20921359950047247 1105 100-101 0.21855207144956523 1106 1 0.3159970344448837 1106 2 0.3769216366679551 1106 3 0.37256949439807485 1106 4 0.46182395998173575 1106 5 0.31075868041520494 1106 6 0.3665642253506576 1106 7 0.3477879840479261 1106 8 0.43859354451740984 1106 9 0.5021778300538458 1106 10-11 0.6242390245949707 1106 12-13 0.4961199599216215 1106 14-15 0.5104892323439998 1106 16-17 0.6806029586889863 1106 18-19 0.63228919252483 1106 20-21 0.6053638053062116 1106 22-23 0.4170089495061404 1106 24-25 0.39175923195480067 1106 26-27 0.3587338737870809 1106 28-29 0.2743599472454008 1106 30-31 0.45696950561163874 1106 32-33 0.537067799589984 1106 34-35 0.6689434460068071 1106 36-37 0.5187890761985017 1106 38-39 0.32783095004671736 1106 40-41 0.4464835967753302 1106 42-43 0.421241462840662 1106 44-45 0.46114789229357456 1106 46-47 0.5718183414110527 1106 48-49 0.5158804277440723 1106 50-51 0.5845697040802023 1106 52-53 0.4777774772100756 1106 54-55 0.5104473483675704 1106 56-57 0.45094386461297375 1106 58-59 0.5532206068492584 1106 60-61 0.5925198323690921 1106 62-63 0.6237601431869209 1106 64-65 0.26055121330264797 1106 66-67 0.4169271313533116 1106 68-69 0.557971369253444 1106 70-71 0.5115725381855185 1106 72-73 0.6465620627164377 1106 74-75 0.7799453534294365 1106 76-77 0.778300903799888 1106 78-79 0.76160499563278 1106 80-81 0.9366753929432292 1106 82-83 0.8494075038764812 1106 84-85 0.8968523547499814 1106 86-87 1.2245410510484938 1106 88-89 1.2483988467468379 1106 90-91 1.1334447163483254 1106 92-93 1.3379741616769998 1106 94-95 1.326576883101339 1106 96-97 1.3651344136444372 1106 98-99 1.4894253988742818 1106 100-101 1.6341306827823665 1107 1 0.33485103042610476 1107 2 0.40235801515494884 1107 3 0.36627113247728005 1107 4 0.4057989453247117 1107 5 0.42191455600336525 1107 6 0.36183629482245294 1107 7 0.3624534280430609 1107 8 0.42466731570745964 1107 9 0.48944821584680653 1107 10-11 0.4468882130717944 1107 12-13 0.5029527896545858 1107 14-15 0.49304000578396057 1107 16-17 0.6099837557305321 1107 18-19 0.6514366296749969 1107 20-21 0.6885859456829309 1107 22-23 0.6407164503955869 1107 24-25 0.5210666687040941 1107 26-27 0.565930297973928 1107 28-29 0.595488513161925 1107 30-31 0.5865715861576319 1107 32-33 0.559168990692541 1107 34-35 0.46110912802200943 1107 36-37 0.40270182555584455 1107 38-39 0.6336584832066734 1107 40-41 0.7136658900862187 1107 42-43 0.6404848095532358 1107 44-45 0.5937872092010181 1107 46-47 0.7944188234643335 1107 48-49 0.8586539879731774 1107 50-51 0.7554849539010675 1107 52-53 0.7658209522029367 1107 54-55 0.8173483270058952 1107 56-57 0.8355821993744286 1107 58-59 0.7431413837467815 1107 60-61 0.8170469240644422 1107 62-63 0.8464606437807092 1107 64-65 0.9287151515076246 1107 66-67 0.958295521285315 1107 68-69 0.9104975389342016 1107 70-71 0.8550754921399104 1107 72-73 0.8917094096695273 1107 74-75 0.9410434527765155 1107 76-77 0.9413032639568257 1107 78-79 0.9750916640031697 1107 80-81 1.1604444282854516 1107 82-83 1.1914993746140432 1107 84-85 1.1974253066498832 1107 86-87 1.2593170365768778 1107 88-89 1.1262083605344628 1107 90-91 1.1731740248874836 1107 92-93 1.2613755938062 1107 94-95 1.322595283305784 1107 96-97 1.2916213876827136 1107 98-99 1.4821793662930922 1107 100-101 1.489694489700483 1108 1 0.0315714003010612 1108 2 0.18721827427458493 1108 3 0.12430568305996914 1108 4 0.10274380870688304 1108 5 0.05241557177320999 1108 6 0.041933825861164564 1108 7 0.054720726796212205 1108 8 0.09841730743254828 1108 9 -0.033556819583445474 1108 10-11 0.046604899539843814 1108 12-13 0.07074632416370008 1108 14-15 0.11443589200372628 1108 16-17 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-2.601824441141762 2316 74-75 -2.9057894421166814 2316 76-77 -3.150463967229136 2316 78-79 -3.444795753032121 2316 80-81 -3.1200850623077905 2316 82-83 -3.7384947213842317 2316 84-85 -3.7043520094732507 2316 86-87 -4.264682881571662 2316 88-89 -3.9084251553601845 2316 90-91 -4.314381297515425 2316 92-93 -4.138033145651065 2316 94-95 -4.460233838049003 2316 96-97 -4.84854586833773 2316 98-99 -5.104202992112601 2316 100-101 -5.572577265057657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6050.0 3 2395.0 4 2410.0 5 2641.0 6 2896.0 7 3443.0 8 4062.0 9 5267.0 10 6137.0 11 7588.0 12 9095.0 13 10430.0 14 11318.0 15 13122.0 16 15330.0 17 19217.0 18 17901.0 19 13563.0 20 13990.0 21 15019.0 22 16619.0 23 18738.0 24 20982.0 25 23931.0 26 27851.0 27 32323.0 28 38394.0 29 45596.0 30 54804.0 31 67061.0 32 84012.0 33 108470.0 34 143946.0 35 199840.0 36 294865.0 37 414708.0 38 344589.0 39 56679.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42050683509051 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22.320986041535235 26.979296780038318 28.743383983011846 21.956333195414597 20-21 23.06473231123614 26.175111264840677 28.717551971121736 22.042604452801445 22-23 22.74305112462024 26.01179952804647 28.73570671281814 22.509442634515146 24-25 22.59699807940527 26.22651983867464 28.748050383716667 22.428431698203422 26-27 22.453950642398162 26.32031527446877 28.659129593299156 22.566604489833917 28-29 22.342236602799982 26.48683588671341 28.720974495622382 22.44995301486422 30-31 22.52616369211192 26.238443876067457 28.646108092614035 22.58928433920659 32-33 22.38747096781312 26.30663230310063 28.646401296081415 22.65949543300484 34-35 22.708207240989566 26.331023693215922 28.497325597192113 22.463443468602403 36-37 22.696780976825583 26.16987525391594 28.556346065391875 22.576997703866603 38-39 22.516012692925223 26.387469816375532 28.577884382208296 22.518633108490953 40-41 22.66627619748607 26.278928318409534 28.518946413621798 22.535849070482598 42-43 22.465775403159167 26.346888473453223 28.326107649019537 22.861228474368072 44-45 22.596347345786207 26.39307579412647 28.299783819691143 22.710793040396183 46-47 22.766912474893957 26.57114762147096 27.948537953626968 22.713401950008112 48-49 22.79507525625189 26.67603242034091 27.74199984278038 22.78689248062682 50-51 22.667973787101804 26.813928417916898 27.77160483895066 22.746492956030647 52-53 22.811172881293594 26.941180662267794 27.474309567839867 22.773336888598745 54-55 22.764487796266057 26.768079401509464 27.536237880860952 22.931194921363524 56-57 22.72017598907654 27.024000809145182 27.456916467253755 22.798906734524525 58-59 22.886423432025673 26.798848479526814 27.48770119312563 22.82702689532188 60-61 22.764808452870714 26.693578379789827 27.870333720873035 22.671279446466425 62-63 22.59705277521216 26.73376913850246 27.89312459801272 22.77605348827266 64-65 22.591147464489755 26.842332097078387 27.708737261536303 22.857783176895555 66-67 22.571467523811776 26.96165400621156 27.447786974211386 23.019091495765277 68-69 22.470860584046175 26.8575651872771 27.72532615196901 22.946248076707715 70-71 22.604725934222373 26.758805786848843 27.633916610224844 23.002551668703937 72-73 22.6435218025521 26.777825946441453 27.537011079763168 23.041641171243278 74-75 22.560420522977868 27.03077600705469 27.334407353359026 23.074396116608415 76-77 22.7109539199769 26.937963911944152 27.34856750842703 23.002514659651922 78-79 22.615172285678355 26.965830250446654 27.297496820934768 23.12150064294022 80-81 22.60240902885521 27.023263356561806 27.341407900593346 23.032919713989642 82-83 22.661106982835367 27.005629271845734 27.408304762395254 22.924958982923656 84-85 22.656358653037383 26.999756099971883 27.226001536041462 23.117883710949272 86-87 22.572865647990735 27.00097440833089 27.359606337194947 23.066553606483424 88-89 22.72425296536125 26.866847857956085 27.17202464134082 23.236874535341844 90-91 22.63114349613892 26.891550883395965 27.354967570259838 23.122338050205276 92-93 22.542099709274986 26.86365357848391 27.361356039149342 23.23289067309176 94-95 22.535737725268067 26.846540133031578 27.373563106093084 23.244159035607268 96-97 22.499749420909424 26.86033135291559 27.347029511320198 23.292889714854788 98-99 22.4087451767681 26.873607583163313 27.198463647875155 23.51918359219343 100-101 22.44440733436291 26.833740240776955 27.24582316064724 23.47602926421289 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 202.0 2 183.5 3 257.0 4 233.5 5 220.5 6 405.5 7 908.5 8 1645.5 9 2075.5 10 1881.0 11 1690.0 12 1749.5 13 1809.5 14 1972.5 15 2077.0 16 2189.0 17 2365.5 18 2510.0 19 2709.0 20 3032.0 21 3583.5 22 4375.0 23 5415.0 24 6647.0 25 8377.5 26 10856.5 27 13637.0 28 16291.0 29 19355.5 30 23380.0 31 27476.5 32 31176.5 33 34924.0 34 39311.0 35 43239.0 36 48082.5 37 53328.5 38 56756.5 39 59311.0 40 61357.0 41 64252.0 42 67013.5 43 70456.5 44 75319.0 45 78974.0 46 81372.0 47 83690.5 48 86683.0 49 86810.5 50 83860.5 51 81749.5 52 80544.5 53 79528.5 54 77314.5 55 72767.5 56 66613.0 57 60300.0 58 55449.0 59 49632.5 60 41627.5 61 33636.5 62 26254.0 63 20495.0 64 15475.5 65 11108.0 66 8083.0 67 5990.0 68 4347.0 69 3075.5 70 2112.5 71 1335.0 72 828.0 73 534.5 74 350.0 75 184.5 76 116.0 77 74.0 78 42.0 79 32.0 80 29.5 81 23.0 82 15.0 83 10.5 84 13.0 85 12.5 86 9.0 87 7.0 88 4.5 89 4.0 90 4.0 91 2.5 92 1.5 93 2.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03636271695233542 2 0.026892780552612162 3 0.023950664389591345 4 0.04486727148606747 5 0.09364204100114697 6 0.11285773719087668 7 0.05709544178862275 8 0.08725213245958613 9 0.07088661130278283 10-11 0.07228871353672243 12-13 0.07033496452221642 14-15 0.06449670276122199 16-17 0.05160195926548232 18-19 0.06065816057978077 20-21 0.055762295402253935 22-23 0.033397615506791 24-25 0.009079186596822055 26-27 0.002643307490214016 28-29 0.006849614192032841 30-31 0.004849894612479629 32-33 0.006114085151277637 34-35 0.0067346877794148404 36-37 0.005815276478470835 38-39 0.0035627187911580213 40-41 0.00661976136679684 42-43 0.003608689356205222 44-45 0.0017238961892700104 46-47 0.001471058081510409 48-49 1.8388226018880113E-4 50-51 0.0018388226018880112 52-53 0.0058612470435180355 54-55 0.010711141655997665 56-57 0.007814996058024047 58-59 0.004321233114436826 60-61 0.002022704862076812 62-63 0.007998878318212848 64-65 0.0024134546649780147 66-67 0.022456621025557337 68-69 0.044752345073449475 70-71 0.05688857424591034 72-73 0.0035857040736816215 74-75 0.013745198949112884 76-77 0.021054518791617727 78-79 0.024387384757539748 80-81 0.07389768331337444 82-83 0.027628309593367366 84-85 0.010734126938521264 86-87 0.02997280841077458 88-89 0.04557981524429907 90-91 0.04192515532304665 92-93 0.04199411117061745 94-95 0.011837420499654071 96-97 0.015767903811189695 98-99 0.036937349015425426 100-101 0.07318513955514284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175305.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.212247360260534 #Duplication Level Percentage of deduplicated Percentage of total 1 80.42411873918138 50.03365168732916 2 11.289590425108893 14.047015842458066 3 3.461465339788155 6.460366138436068 4 1.5466860384447538 3.8489125764954584 5 0.8318480545306928 2.5875568467307475 6 0.5185863422146282 1.93574530797055 7 0.3488578248043227 1.519226050320227 8 0.2565304137690057 1.2767466845461892 9 0.19770273440659936 1.1069578275032925 >10 1.04726748499789 11.693153077186615 >50 0.05601245081326856 2.3399271937886863 >100 0.0205943095712942 2.142053441578296 >500 3.699211845601917E-4 0.1626142971272979 >1k 2.2195271073610084E-4 0.28141064609007915 >5k 1.4796847382406723E-4 0.5646623824391709 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7020 0.3227133666313459 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5248 0.24125352536770706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3361 0.15450706912364015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.5970565047200275E-5 4.5970565047200275E-5 5 0.0 0.0 0.0 4.5970565047200275E-5 4.5970565047200275E-5 6 4.5970565047200275E-5 0.0 0.0 4.5970565047200275E-5 4.5970565047200275E-5 7 4.5970565047200275E-5 0.0 0.0 9.194113009440055E-5 4.5970565047200275E-5 8 4.5970565047200275E-5 0.0 0.0 9.194113009440055E-5 9.194113009440055E-5 9 4.5970565047200275E-5 0.0 0.0 9.194113009440055E-5 9.194113009440055E-5 10-11 4.5970565047200275E-5 0.0 0.0 1.149264126180007E-4 9.194113009440055E-5 12-13 4.5970565047200275E-5 0.0 0.0 1.838822601888011E-4 9.194113009440055E-5 14-15 4.5970565047200275E-5 0.0 0.0 1.838822601888011E-4 1.6089697766520097E-4 16-17 4.5970565047200275E-5 0.0 0.0 2.0686754271240123E-4 1.838822601888011E-4 18-19 4.5970565047200275E-5 0.0 0.0 2.298528252360014E-4 1.838822601888011E-4 20-21 4.5970565047200275E-5 0.0 0.0 2.528381077596015E-4 2.298528252360014E-4 22-23 4.5970565047200275E-5 0.0 0.0 2.7582339028320166E-4 2.7582339028320166E-4 24-25 4.5970565047200275E-5 4.5970565047200275E-5 0.0 3.2179395533040193E-4 2.988086728068018E-4 26-27 4.5970565047200275E-5 4.5970565047200275E-5 0.0 5.056762155192031E-4 3.2179395533040193E-4 28-29 4.5970565047200275E-5 4.5970565047200275E-5 0.0 0.0010573229960856062 3.2179395533040193E-4 30-31 4.5970565047200275E-5 4.5970565047200275E-5 0.0 0.0019997195795532123 3.2179395533040193E-4 32-33 4.5970565047200275E-5 4.5970565047200275E-5 0.0 0.0032409248358276194 3.2179395533040193E-4 34-35 4.5970565047200275E-5 4.5970565047200275E-5 0.0 0.004482130092102027 3.2179395533040193E-4 36-37 4.5970565047200275E-5 4.5970565047200275E-5 0.0 0.0062749821289428374 3.4477923785400204E-4 38-39 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.009125157161869256 3.677645203776022E-4 40-41 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.012503993692838476 3.677645203776022E-4 42-43 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.015377154008288494 3.677645203776022E-4 44-45 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.017468814717936106 3.9074980290120236E-4 46-47 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.021721091984802134 4.367203679484027E-4 48-49 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.02480111984296455 4.597056504720028E-4 50-51 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.027996074113744968 4.597056504720028E-4 52-53 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.03447792378540021 5.286614980428032E-4 54-55 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.045441903549157475 6.206026281372038E-4 56-57 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.06199130696614957 6.435879106608039E-4 58-59 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.0838043400810461 6.435879106608039E-4 60-61 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.10913412142205346 7.125437582316043E-4 62-63 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.12290230565368995 7.355290407552044E-4 64-65 4.5970565047200275E-5 9.194113009440055E-5 0.0 0.14048604678424403 7.355290407552044E-4 66-67 9.194113009440055E-5 9.194113009440055E-5 0.0 0.1813768643937287 7.355290407552044E-4 68-69 9.194113009440055E-5 9.194113009440055E-5 0.0 0.23817349750954464 7.355290407552044E-4 70-71 9.194113009440055E-5 1.149264126180007E-4 0.0 0.29434952799722336 7.814996058024047E-4 72-73 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.3577659224798362 7.814996058024047E-4 74-75 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.4206996260294533 8.044848883260048E-4 76-77 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.4723475558599829 8.27470170849605E-4 78-79 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.5217429280031995 8.504554533732052E-4 80-81 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.5730230933133516 9.423965834676057E-4 82-83 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.6383242809628995 0.001011352431038406 84-85 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.7620310715049154 0.001011352431038406 86-87 9.194113009440055E-5 1.3791169514160083E-4 0.0 0.9388338646764477 0.001011352431038406 88-89 9.194113009440055E-5 1.3791169514160083E-4 0.0 1.1472644066004538 0.0010573229960856064 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8370 0.0 36.34384 1 GGTATCA 5150 0.0 35.994293 1 TCAACGC 10085 0.0 29.55479 4 TATCAAC 10135 0.0 29.5497 2 ATCAACG 10100 0.0 29.416765 3 CAACGCA 10225 0.0 29.102966 5 AACGCAG 10400 0.0 28.655666 6 ACGCAGA 11795 0.0 25.061024 7 TACATGG 7815 0.0 24.818039 2 GTACATG 8005 0.0 24.64127 1 CGCAGAG 11885 0.0 24.591911 8 ACATGGG 7880 0.0 23.286133 3 GCAGAGT 12835 0.0 22.141735 9 GAGTACT 6260 0.0 21.674204 12-13 AGAGTAC 10080 0.0 20.412855 10-11 GTACTTT 6735 0.0 19.863562 14-15 CTAGACG 120 0.0047431416 19.80729 4 AGTACTT 6645 0.0 19.309906 12-13 CATGGGG 4760 0.0 18.575575 4 CAGAGTA 12415 0.0 18.142954 10-11 >>END_MODULE