FastQCFastQC Report
Thu 26 May 2016
SRR936996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175305
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72400.33282689094173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59360.2728812741201808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37370.17179200158138744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61100.032.581841
GTATCAA92100.031.0039941
ATCAACG108200.025.9431843
TCAACGC109550.025.623484
TATCAAC112500.025.0782362
CAACGCA112600.025.013785
AACGCAG116700.024.1371976
TACATGG73100.021.181822
ACGCAGA131900.021.1755897
CGCAGAG132150.020.9572538
GTACATG73250.020.950661
GAGTACT70250.020.55654712-13
AGAGTAC110250.019.54031810-11
ACATGGG73500.019.5156333
AGTACTT74650.019.31309112-13
GCAGAGT142350.019.0217429
GTACTTT75600.018.94407814-15
CAGAGTA137800.016.2714310-11
ACTTTTT88700.016.22620616-17
TACTTTT86900.016.20738214-15
Produced by FastQC (version 0.11.5)