##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936996_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175305 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09738956146379 31.0 31.0 34.0 31.0 34.0 2 32.229891900216295 33.0 31.0 34.0 30.0 34.0 3 32.36620198087165 34.0 31.0 34.0 31.0 34.0 4 36.039047397951094 37.0 35.0 37.0 35.0 37.0 5 35.86765074322911 37.0 35.0 37.0 35.0 37.0 6 35.95002034197503 37.0 35.0 37.0 35.0 37.0 7 35.908763598667775 37.0 35.0 37.0 35.0 37.0 8 35.85340170688708 37.0 35.0 37.0 35.0 37.0 9 37.54958913807489 39.0 37.0 39.0 35.0 39.0 10-11 37.45480886588318 39.0 37.0 39.0 34.5 39.0 12-13 37.351297174419216 39.0 37.0 39.0 34.0 39.0 14-15 38.551294416185314 40.0 38.0 41.0 34.0 41.0 16-17 38.53850448557789 40.0 38.0 41.0 34.0 41.0 18-19 38.641057920613434 40.0 38.0 41.0 34.0 41.0 20-21 38.63427013683139 40.0 38.0 41.0 34.0 41.0 22-23 38.54257747764106 40.0 38.0 41.0 34.0 41.0 24-25 38.44803165533109 40.0 38.0 41.0 34.0 41.0 26-27 38.25275536074252 40.0 38.0 41.0 34.0 41.0 28-29 38.098895787027566 40.0 38.0 41.0 33.0 41.0 30-31 38.12187026646838 40.0 38.0 41.0 34.0 41.0 32-33 38.0157998533539 40.0 37.0 41.0 33.0 41.0 34-35 37.906794449513974 40.0 37.0 41.0 33.0 41.0 36-37 37.77896249031745 40.0 37.0 41.0 33.0 41.0 38-39 37.61763637742753 40.0 37.0 41.0 33.0 41.0 40-41 37.31081802321973 40.0 36.5 41.0 31.5 41.0 42-43 37.135831527073215 39.0 36.0 41.0 31.0 41.0 44-45 36.93833048698919 39.0 36.0 41.0 31.0 41.0 46-47 36.6199905300636 39.0 36.0 40.5 30.5 41.0 48-49 36.29025102227044 39.0 35.0 40.0 30.0 41.0 50-51 36.02056286359844 39.0 35.0 40.0 29.5 41.0 52-53 35.7510073300066 38.5 35.0 40.0 28.5 41.0 54-55 35.35301624369916 38.0 34.5 40.0 28.0 41.0 56-57 35.142289012345394 38.0 34.0 40.0 27.5 41.0 58-59 34.805747469895024 38.0 34.0 40.0 26.5 41.0 60-61 34.41517718205033 37.0 33.5 40.0 26.0 41.0 62-63 34.014094575243476 37.0 33.0 39.0 26.0 40.5 64-65 33.67750453384698 36.0 33.0 39.0 26.0 40.0 66-67 33.315427491777015 36.0 32.0 39.0 25.0 40.0 68-69 32.85006332445336 35.0 32.0 38.5 23.5 40.0 70-71 32.39386798632836 35.0 31.0 38.0 22.5 40.0 72-73 31.963437540942536 35.0 31.0 37.0 22.0 39.0 74-75 31.467497661247503 34.5 31.0 36.5 20.5 39.0 76-77 30.052207851312804 33.0 29.5 35.0 19.5 37.5 78-79 30.7457501361878 34.0 30.0 36.0 20.0 37.5 80-81 30.613234236118615 34.0 30.0 35.0 19.0 37.0 82-83 30.25533109150211 34.0 30.0 35.0 18.0 37.0 84-85 29.805436938728132 34.0 29.5 35.0 14.5 36.0 86-87 29.41286141483608 34.0 29.0 35.0 8.5 36.0 88-89 29.013205964221108 34.0 29.0 35.0 6.0 35.0 90-91 28.537732180085094 33.0 29.0 35.0 2.0 35.0 92-93 28.01272350314094 33.0 27.0 35.0 2.0 35.0 94-95 27.41892240398473 33.0 26.5 34.0 2.0 35.0 96-97 26.594364928136514 32.0 25.0 34.0 2.0 35.0 98-99 25.66182374425655 32.0 24.0 34.0 2.0 35.0 100-101 22.40952648019473 28.0 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14120940449797104 1101 2 0.22321860442475128 1101 3 0.16066618824246603 1101 4 0.11020433092504334 1101 5 0.020696449861141275 1101 6 -0.0016973744897939014 1101 7 0.11823043404616129 1101 8 0.12784376576932743 1101 9 0.1346652064116327 1101 10-11 0.18268378239830696 1101 12-13 0.18034859506041556 1101 14-15 0.2987507846322828 1101 16-17 0.3327640281113773 1101 18-19 0.16684270866078776 1101 20-21 0.08118255285328502 1101 22-23 0.08280032828496076 1101 24-25 0.11869623587156042 1101 26-27 0.1575739177494242 1101 28-29 0.13876609591248013 1101 30-31 0.20511891959278472 1101 32-33 0.23638400690463612 1101 34-35 0.06424015974432962 1101 36-37 0.015804892700082007 1101 38-39 0.17582388178238517 1101 40-41 0.19229081750923172 1101 42-43 0.1590249793505052 1101 44-45 0.0705571239415903 1101 46-47 -0.15344285623800147 1101 48-49 -0.030516206960726322 1101 50-51 0.05282990207074789 1101 52-53 0.07619847165683069 1101 54-55 -0.03782369675739261 1101 56-57 -0.042592656833470244 1101 58-59 0.201001009871824 1101 60-61 0.12194044992575215 1101 62-63 0.10017181763871719 1101 64-65 0.1714499786306689 1101 66-67 0.11941585857432102 1101 68-69 -0.29520005063491084 1101 70-71 -0.23045997806467255 1101 72-73 0.0039167339505716825 1101 74-75 -0.13153741600067903 1101 76-77 -0.02850613747276398 1101 78-79 0.005917115436542275 1101 80-81 -0.06408479458848504 1101 82-83 0.2286569108664338 1101 84-85 0.11920744211365175 1101 86-87 -0.23616654011889082 1101 88-89 -0.16290984898319394 1101 90-91 -0.10570033622065367 1101 92-93 -0.24142424881380364 1101 94-95 -0.21423463195911197 1101 96-97 -0.31130138924565287 1101 98-99 -0.2993785730629348 1101 100-101 0.005615057499323228 1102 1 -0.09750597304483577 1102 2 -0.02820723743251108 1102 3 0.06419020847003054 1102 4 0.10941778340176711 1102 5 4.868475165835662E-4 1102 6 0.16452274388741017 1102 7 0.16543406006615413 1102 8 0.19939804113997184 1102 9 0.24459869106856758 1102 10-11 0.21217931452105177 1102 12-13 0.30450458287790383 1102 14-15 0.27629467326576673 1102 16-17 0.2191079109984031 1102 18-19 0.3317249779401763 1102 20-21 0.38209444516451185 1102 22-23 0.09999662756090544 1102 24-25 0.18353612496476757 1102 26-27 0.29763504578068023 1102 28-29 0.10656972480571625 1102 30-31 0.12644082224765185 1102 32-33 0.07857348225229543 1102 34-35 0.2870521928439729 1102 36-37 0.32210589258514943 1102 38-39 0.4719823693125491 1102 40-41 0.4717073357255259 1102 42-43 0.33018885419247823 1102 44-45 0.37224478915248227 1102 46-47 0.33851927455155817 1102 48-49 0.3394969380800674 1102 50-51 0.3447629413765654 1102 52-53 0.29367347453005976 1102 54-55 0.524579331630143 1102 56-57 0.2975891469821832 1102 58-59 0.14080498355301785 1102 60-61 0.19478229281737924 1102 62-63 0.27342956100154936 1102 64-65 0.4270384167412331 1102 66-67 0.29136268939836185 1102 68-69 0.4596251849702 1102 70-71 0.3212546237052365 1102 72-73 0.3002117000464217 1102 74-75 0.350562342647752 1102 76-77 0.41084530201694847 1102 78-79 0.019832541824314376 1102 80-81 0.4146963799891523 1102 82-83 0.5992824136822037 1102 84-85 0.46524166231822406 1102 86-87 0.49716511716188094 1102 88-89 0.3902970381562163 1102 90-91 0.48696142679509435 1102 92-93 0.41203153213091426 1102 94-95 0.3263982694315324 1102 96-97 0.3968948669317989 1102 98-99 0.33815051842080024 1102 100-101 0.5047925945640301 1103 1 0.14885627365787713 1103 2 0.2639882373261955 1103 3 0.15168515515758685 1103 4 0.11421558773751173 1103 5 0.057146797488790924 1103 6 0.00821463079427076 1103 7 0.16329556140528467 1103 8 0.20824670706549142 1103 9 0.18143575964202086 1103 10-11 0.17131365051579905 1103 12-13 0.1799186386664715 1103 14-15 0.23790671151934362 1103 16-17 0.21741363008476355 1103 18-19 0.20850042768498867 1103 20-21 0.15687162566883472 1103 22-23 0.1691600516854166 1103 24-25 0.27265271119954804 1103 26-27 0.3192949641696856 1103 28-29 0.15692803624136076 1103 30-31 0.30809871931167976 1103 32-33 0.26452322074337786 1103 34-35 0.2546231918838373 1103 36-37 0.22192523666520003 1103 38-39 0.16675598091495658 1103 40-41 0.25515757954785556 1103 42-43 0.13670778516422644 1103 44-45 0.1159036716124362 1103 46-47 0.09639170367885441 1103 48-49 0.13664260612797818 1103 50-51 -0.028318536969983654 1103 52-53 -0.13690917239500067 1103 54-55 0.2525206753059237 1103 56-57 0.06167920332661225 1103 58-59 0.026189066882132295 1103 60-61 -0.03188893235822121 1103 62-63 0.18508170992266315 1103 64-65 0.03749751017696923 1103 66-67 0.17523709563415224 1103 68-69 0.06631908688540022 1103 70-71 0.11503557524397223 1103 72-73 0.21346843358525192 1103 74-75 -0.05870543237877612 1103 76-77 0.09163529266831816 1103 78-79 0.1085672871373653 1103 80-81 0.10317356428345548 1103 82-83 0.33432397717707474 1103 84-85 0.04299982341782638 1103 86-87 -0.1749188057245732 1103 88-89 0.21139951301598714 1103 90-91 0.010805481572997877 1103 92-93 -0.012217735138779773 1103 94-95 0.36680471366822687 1103 96-97 0.5986981098950608 1103 98-99 0.22877457092471687 1103 100-101 0.3500301634154148 1104 1 -0.03907312162926502 1104 2 0.06852346779789542 1104 3 -0.002583328878600355 1104 4 -0.07228059618844185 1104 5 -0.21730892693145165 1104 6 -0.0632706312912461 1104 7 0.04137844990357564 1104 8 0.0014920163939606823 1104 9 0.0023800312024135906 1104 10-11 0.08563486547586052 1104 12-13 0.12945772617877793 1104 14-15 0.15528611909645207 1104 16-17 0.12855282519226563 1104 18-19 0.0747350903446602 1104 20-21 0.020687483284511643 1104 22-23 0.022672432090111272 1104 24-25 0.05104300351529645 1104 26-27 0.09256273554225203 1104 28-29 0.0010712862657058508 1104 30-31 0.0015621562672336609 1104 32-33 -0.04004176845845819 1104 34-35 0.017516528865513692 1104 36-37 -0.03297243195085997 1104 38-39 -0.02432907649234295 1104 40-41 0.10231264494749581 1104 42-43 0.011705273063633115 1104 44-45 0.10977028138968592 1104 46-47 -0.0196984165773344 1104 48-49 0.08794039594322811 1104 50-51 0.1520097829835123 1104 52-53 0.2094268709151592 1104 54-55 0.28934080426557784 1104 56-57 0.1862882818262861 1104 58-59 0.15867091405676348 1104 60-61 0.19060171103325274 1104 62-63 0.2431248376656896 1104 64-65 0.2037328684952726 1104 66-67 -0.12866964591629682 1104 68-69 0.025319131716734944 1104 70-71 0.10612094183287724 1104 72-73 0.08906148256426505 1104 74-75 -0.0761993144476385 1104 76-77 0.10495143550403796 1104 78-79 0.2375616430270462 1104 80-81 0.29180928496260705 1104 82-83 0.27430363302702077 1104 84-85 0.15541617046800482 1104 86-87 0.14927960229930903 1104 88-89 0.28833703823361034 1104 90-91 0.4196748198779723 1104 92-93 0.07227773457765352 1104 94-95 0.10566814660441892 1104 96-97 0.21525687217923561 1104 98-99 0.04154817732725391 1104 100-101 0.5437795933618155 1105 1 -0.04808367746332465 1105 2 0.03621768820051585 1105 3 -0.007034307237724136 1105 4 0.02489563655420568 1105 5 -0.09041997610671615 1105 6 0.015981515562657478 1105 7 0.0474875861490105 1105 8 0.06025697968745902 1105 9 0.06901327787840472 1105 10-11 0.044442111082027225 1105 12-13 0.024569608634273266 1105 14-15 0.1287521843301178 1105 16-17 -0.11846260158957733 1105 18-19 -0.09421073090404519 1105 20-21 0.09213920138594034 1105 22-23 0.0015329404100654642 1105 24-25 0.02206796672636102 1105 26-27 -0.036291938547833524 1105 28-29 -0.1476440258291305 1105 30-31 -0.014567349050317091 1105 32-33 -0.07095288174682679 1105 34-35 -0.086038752523514 1105 36-37 -0.13489729975323428 1105 38-39 -0.10197649305413137 1105 40-41 0.0921376212221503 1105 42-43 0.007915718808305883 1105 44-45 -0.07763572488654802 1105 46-47 -0.2028314836288061 1105 48-49 -0.12079495229212256 1105 50-51 -0.1475702317352372 1105 52-53 -0.03762232464283244 1105 54-55 0.2250867501892415 1105 56-57 0.0016800107273766685 1105 58-59 -0.014364654769558172 1105 60-61 -0.09351783233679356 1105 62-63 0.07833075268695922 1105 64-65 0.077432017553825 1105 66-67 -0.03036176282538605 1105 68-69 -0.26998398030587367 1105 70-71 -0.2187334957172382 1105 72-73 -0.2744772283436241 1105 74-75 -0.27948917008532703 1105 76-77 -0.2400210278822037 1105 78-79 -0.048475110718673164 1105 80-81 -0.1754599720387091 1105 82-83 -0.19682057550738108 1105 84-85 -0.5499060740236423 1105 86-87 -0.4516962519159726 1105 88-89 -0.054135188137045986 1105 90-91 -0.2028557392790553 1105 92-93 -0.18260724389752525 1105 94-95 -0.15926454889539343 1105 96-97 0.06124247811949246 1105 98-99 0.20812281218848838 1105 100-101 0.5454382268646896 1106 1 -0.0429280509669141 1106 2 -0.007117077592347698 1106 3 0.11856333534315411 1106 4 0.12970624494023042 1106 5 0.019017067296807966 1106 6 0.1298411754558373 1106 7 0.1753603837424791 1106 8 0.20317551366744624 1106 9 0.18726976998964773 1106 10-11 0.2475957730625069 1106 12-13 0.2557470903704129 1106 14-15 0.41754155138765014 1106 16-17 0.31472666724215514 1106 18-19 0.2108577201979216 1106 20-21 0.26828138322645145 1106 22-23 0.16598563854991966 1106 24-25 0.20600927681292092 1106 26-27 0.3571711346917681 1106 28-29 0.36459981971081135 1106 30-31 0.36211077729260666 1106 32-33 0.27965427645809626 1106 34-35 0.24379794708971758 1106 36-37 0.23523776071701974 1106 38-39 0.3300193323495151 1106 40-41 0.47551290515609423 1106 42-43 0.5719577103363349 1106 44-45 0.30248767127037013 1106 46-47 0.32535993389222284 1106 48-49 0.1626668931250279 1106 50-51 0.24258324607187376 1106 52-53 0.21719994805653187 1106 54-55 0.5377124485132683 1106 56-57 0.5072451160131592 1106 58-59 0.4774414720645055 1106 60-61 0.30331438572446956 1106 62-63 0.4994204451174298 1106 64-65 0.33264780735061805 1106 66-67 0.3187634136740911 1106 68-69 0.31294499016500055 1106 70-71 0.38309902929965034 1106 72-73 0.3885708409055617 1106 74-75 0.30609680818221463 1106 76-77 0.48406270960935416 1106 78-79 0.4554612880780624 1106 80-81 0.5197263500191234 1106 82-83 0.7039652308650197 1106 84-85 0.5845036503302161 1106 86-87 0.6084001321468939 1106 88-89 0.6801265836107611 1106 90-91 0.4039144737481415 1106 92-93 0.5602695441189276 1106 94-95 0.7171980946063563 1106 96-97 0.829928708090641 1106 98-99 0.6176329110281955 1106 100-101 0.799139497660935 1107 1 0.19912002407705387 1107 2 0.30636768661744895 1107 3 0.20410965404953174 1107 4 0.13837558745362344 1107 5 0.042929115121303596 1107 6 0.03109180808814216 1107 7 0.0740558332997665 1107 8 0.1031319184118118 1107 9 0.20585658217427039 1107 10-11 0.17249347454023933 1107 12-13 0.2727327039360503 1107 14-15 0.29531558765689425 1107 16-17 0.34130854865303917 1107 18-19 0.2685316377076745 1107 20-21 0.22560784153785818 1107 22-23 0.24540246431421053 1107 24-25 0.2688804503270532 1107 26-27 0.2595701765983449 1107 28-29 0.22502745409193636 1107 30-31 0.32773406417444306 1107 32-33 0.382144818291998 1107 34-35 0.377874303929417 1107 36-37 0.40199482490226757 1107 38-39 0.4373525554419686 1107 40-41 0.47056910294827503 1107 42-43 0.45756997313053915 1107 44-45 0.4376862802309418 1107 46-47 0.34166646365976305 1107 48-49 0.3821238064807133 1107 50-51 0.35338625063684503 1107 52-53 0.4377313090803696 1107 54-55 0.5502041428654252 1107 56-57 0.4458920595848568 1107 58-59 0.3162304259286941 1107 60-61 0.46777874033930544 1107 62-63 0.41749914708122304 1107 64-65 0.44428262943687713 1107 66-67 0.48043767192837805 1107 68-69 0.2742686824326057 1107 70-71 0.17497177124078434 1107 72-73 0.45313000267402614 1107 74-75 0.46892448665969155 1107 76-77 0.2176521024326732 1107 78-79 0.45891363144832553 1107 80-81 0.3977053340993386 1107 82-83 0.589937079353966 1107 84-85 0.6684881564761405 1107 86-87 0.836186745897944 1107 88-89 0.7076974259011628 1107 90-91 0.6314663746516125 1107 92-93 0.8011120850709084 1107 94-95 1.0579331005944397 1107 96-97 0.9315208870015645 1107 98-99 1.1562426733588858 1107 100-101 1.5997103449622863 1108 1 0.052589821425421235 1108 2 0.07979201672921477 1108 3 0.05044141456538398 1108 4 0.04318547280033158 1108 5 -0.15022759069251634 1108 6 -0.05352647312103187 1108 7 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-1.0988271633696414 2316 66-67 -1.1166900161189588 2316 68-69 -0.9796855918995462 2316 70-71 -0.9712923722615656 2316 72-73 -1.157314360073979 2316 74-75 -0.7952236663458052 2316 76-77 -0.6973086472337009 2316 78-79 -0.7487072511848183 2316 80-81 -0.9240860130385222 2316 82-83 -0.9794778431440925 2316 84-85 -0.9224371905728894 2316 86-87 -0.807643711821477 2316 88-89 -1.5553596843283373 2316 90-91 -1.439777183905779 2316 92-93 -1.2636091676843115 2316 94-95 -1.123818767165222 2316 96-97 -0.8638215090558035 2316 98-99 -1.3241541341550764 2316 100-101 -2.118195569108096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 24.0 5 37.0 6 87.0 7 210.0 8 507.0 9 1011.0 10 1755.0 11 2826.0 12 4280.0 13 5925.0 14 7756.0 15 10526.0 16 13326.0 17 17602.0 18 16878.0 19 11747.0 20 11148.0 21 11662.0 22 12754.0 23 14450.0 24 16304.0 25 19953.0 26 24329.0 27 30623.0 28 37767.0 29 47257.0 30 59266.0 31 73977.0 32 93926.0 33 122423.0 34 165104.0 35 233478.0 36 350026.0 37 476520.0 38 267586.0 39 12249.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.58266087316367 19.460836379520426 15.154930225072993 22.801572522242914 2 15.892525407436114 20.646188091570206 39.603649972945625 23.857636528048054 3 19.739382594368767 24.430790108564533 29.488584542057723 26.341242755008977 4 13.650729437940887 17.21358614079405 36.52683186955393 32.60885255171114 5 14.287343005829243 36.97362130155753 33.89446681745463 14.844568875158604 6 32.21773498428956 36.110062726836006 17.25845341228012 14.413748876594317 7 28.706234217701365 32.21682884345661 20.677046532870623 18.3998904059714 8 26.259903783607356 35.22145170447363 19.53008888408752 18.9885556278315 9 26.067345069930454 17.125698293204074 20.21726618440457 36.5896904524609 10-11 25.03076580065784 26.693865917652925 27.160375211751912 21.11499306993732 12-13 25.94410501990995 23.913480725746076 27.766377639249555 22.376036615094417 14-15 22.9802120185203 25.36593181103003 25.894587457122437 25.75926871332723 16-17 22.40143336221817 28.420635267238385 26.579583092945587 22.598348277597854 18-19 22.40129380212514 27.16414424900726 28.70678630107011 21.727775647797493 20-21 23.21945131138441 26.225113807318834 28.807290104365997 21.748144776930758 22-23 22.856752758503067 26.147027914644315 28.76228465931188 22.233934667540733 24-25 22.757797336819756 26.311868648559688 28.826402316192663 22.10393169842789 26-27 22.631483497804787 26.42633676297286 28.782102159171956 22.160077580050398 28-29 22.49110411898656 26.576991864817085 28.763076617549405 22.168827398646947 30-31 22.707804192975235 26.36023454182287 28.664509114813786 22.267452150388106 32-33 22.64682207490175 26.427881541100252 28.65715718683788 22.26813919716012 34-35 22.81834041663123 26.456381978619092 28.58403764069866 22.141239964051017 36-37 22.864270381210236 26.314164750620556 28.617396603041602 22.204168265127606 38-39 22.745262466820545 26.48136054215351 28.63748782529199 22.135889165733953 40-41 22.924513795047698 26.38517642348163 28.443521247612104 22.246788533858567 42-43 22.644020033972247 26.50954693709618 28.33090072426625 22.515532304665324 44-45 22.8205874348592 26.540991353788556 28.265291768262408 22.373129443089834 46-47 23.01530294320256 26.787103666826724 27.849968314722496 22.347625075248224 48-49 23.10054134962286 26.87123271046254 27.62781661781526 22.40040932209935 50-51 22.940656994606183 27.044402324494122 27.609183048165537 22.405757632734158 52-53 23.136260672390787 27.13916142504646 27.310011619100376 22.414566283462378 54-55 23.069753436874368 26.95828860780442 27.391630139221856 22.580327816099352 56-57 23.045424319731605 27.188669882009354 27.319548411609528 22.446357386649517 58-59 23.153766571561032 26.98640946388786 27.364564114245365 22.49525985030574 60-61 23.086092590408956 26.97041767487554 27.631485492480586 22.312004242234924 62-63 22.912419178000327 26.915926732113427 27.729031101385782 22.442622988500464 64-65 22.952166007103834 27.002173718667894 27.51697520967754 22.528685064550732 66-67 22.99413714263911 27.104573175847335 27.328380923222472 22.57290875829108 68-69 22.833284106463353 27.004841855328166 27.573038692319802 22.588835345888675 70-71 22.938836022617572 26.943456075024137 27.468188295867236 22.649519606491058 72-73 23.024824162184526 26.95267319450191 27.345308693053834 22.677193950259735 74-75 22.93102001740044 27.24407620794559 27.134179909503924 22.690723865150048 76-77 23.025487259817886 27.155510555428496 27.15180992068919 22.667192264064422 78-79 22.99336965321121 27.173021112940233 27.077057381943924 22.756551851904632 80-81 22.946218353342914 27.2638380103064 27.101442845981577 22.688500790369105 82-83 22.999955868196693 27.26375464188421 27.12138360558672 22.61490588433238 84-85 23.001037812938527 27.2284648068783 27.018166898249508 22.752330481933665 86-87 22.935874288459395 27.224345175774804 27.06889517410837 22.770885361657427 88-89 23.081325789312178 27.15162632040483 26.93691364026604 22.830134250016954 90-91 22.99720517003319 27.11483857265305 27.03312444705051 22.85483181026325 92-93 22.93643879823749 27.112542838820303 27.105486357085557 22.84553200585665 94-95 22.910902149353767 27.1360108122769 27.054252162340454 22.89883487602888 96-97 22.971050902840574 27.144105574355 26.99083942865838 22.89400409414605 98-99 22.78274696787973 27.203576713573757 26.80305595614016 23.21062036240635 100-101 22.69708608875741 27.31105088828666 26.8853996428005 23.10646338015543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 373.0 1 284.0 2 226.0 3 268.5 4 244.0 5 234.5 6 407.0 7 897.5 8 1542.5 9 1898.5 10 1796.5 11 1684.5 12 1786.5 13 1841.5 14 1990.0 15 2158.5 16 2331.5 17 2593.5 18 2820.5 19 3005.0 20 3251.5 21 3719.5 22 4407.0 23 5449.0 24 6720.5 25 8404.0 26 10793.5 27 13606.0 28 16423.0 29 19585.5 30 23348.5 31 27407.0 32 31285.5 33 34880.5 34 39112.5 35 43260.0 36 48337.0 37 53482.0 38 56906.0 39 59331.5 40 61628.0 41 64778.5 42 66936.0 43 70057.5 44 75402.0 45 79386.5 46 81437.5 47 83145.5 48 86667.5 49 87025.5 50 83891.0 51 82040.5 52 80741.5 53 79501.5 54 77301.0 55 72893.5 56 66741.0 57 60567.0 58 55301.0 59 49383.0 60 41276.0 61 32943.0 62 25867.5 63 20260.0 64 15259.5 65 10860.0 66 7824.0 67 5913.5 68 4252.5 69 2919.0 70 1973.5 71 1209.0 72 752.0 73 507.0 74 320.5 75 174.0 76 111.0 77 64.5 78 31.5 79 18.0 80 12.5 81 7.5 82 4.0 83 2.0 84 1.0 85 2.0 86 2.0 87 1.5 88 2.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020916607096476126 2 0.0025743516426432154 3 4.137350854248025E-4 4 0.0 5 0.002988086728068018 6 0.0 7 1.8388226018880113E-4 8 0.0 9 4.597056504720028E-5 10-11 0.0 12-13 0.006274982128942838 14-15 0.0032409248358276194 16-17 0.0 18-19 5.05676215519203E-4 20-21 0.0012641905387980076 22-23 0.0014250875164632086 24-25 5.05676215519203E-4 26-27 1.6089697766520097E-4 28-29 2.298528252360014E-5 30-31 0.0 32-33 9.194113009440056E-5 34-35 0.0 36-37 0.0016319550591756098 38-39 0.009263068857010856 40-41 6.206026281372038E-4 42-43 0.0 44-45 7.814996058024047E-4 46-47 2.0686754271240126E-4 48-49 4.597056504720028E-5 50-51 0.00165494034169921 52-53 3.447792378540021E-4 54-55 0.0 56-57 2.528381077596015E-4 58-59 2.0686754271240126E-4 60-61 0.0016779256242228102 62-63 0.0 64-65 2.298528252360014E-5 66-67 2.298528252360014E-5 68-69 1.149264126180007E-4 70-71 2.298528252360014E-4 72-73 2.298528252360014E-4 74-75 0.0033328659659220203 76-77 1.149264126180007E-4 78-79 1.8388226018880113E-4 80-81 0.0025513663601196153 82-83 1.3791169514160083E-4 84-85 0.002643307490214016 86-87 3.447792378540021E-4 88-89 0.0033328659659220203 90-91 0.0017698667543172106 92-93 0.0 94-95 0.0 96-97 1.8388226018880113E-4 98-99 5.516467805664033E-4 100-101 0.0022985282523600137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175305.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.17071912005035 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77688881843075 47.40062050245232 2 12.35146514627836 14.863930800756137 3 3.6497034621320195 6.588158456742632 4 1.6642055619262284 4.005457816987546 5 0.9157380467944741 2.7550308400606913 6 0.5625563145670284 2.0309650795814016 7 0.38293784943328973 1.6129152045080628 8 0.2642542224103194 1.2720293274350913 9 0.20904974349019917 1.1320806057900616 >10 1.1350552160081155 12.254292271434378 >50 0.06576908231920531 2.6740577415880735 >100 0.021304867448920656 2.220287933095574 >500 6.123821491403262E-4 0.20602344062425898 >1k 3.0619107456995366E-4 0.3772720773870462 >5k 1.5309553728497683E-4 0.6068779015567966 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7240 0.33282689094173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5936 0.2728812741201808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3737 0.17179200158138744 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.5970565047200275E-5 0.0 4 0.0 0.0 0.0 4.5970565047200275E-5 0.0 5 0.0 0.0 0.0 4.5970565047200275E-5 0.0 6 9.194113009440055E-5 0.0 0.0 4.5970565047200275E-5 0.0 7 9.194113009440055E-5 0.0 0.0 4.5970565047200275E-5 4.5970565047200275E-5 8 9.194113009440055E-5 0.0 0.0 4.5970565047200275E-5 4.5970565047200275E-5 9 9.194113009440055E-5 0.0 0.0 1.3791169514160083E-4 1.3791169514160083E-4 10-11 9.194113009440055E-5 0.0 0.0 2.0686754271240123E-4 1.3791169514160083E-4 12-13 9.194113009440055E-5 0.0 0.0 2.7582339028320166E-4 1.3791169514160083E-4 14-15 9.194113009440055E-5 0.0 0.0 2.7582339028320166E-4 1.3791169514160083E-4 16-17 9.194113009440055E-5 0.0 0.0 2.7582339028320166E-4 1.3791169514160083E-4 18-19 9.194113009440055E-5 2.2985282523600137E-5 0.0 2.7582339028320166E-4 1.3791169514160083E-4 20-21 9.194113009440055E-5 4.5970565047200275E-5 0.0 2.988086728068018E-4 1.3791169514160083E-4 22-23 9.194113009440055E-5 4.5970565047200275E-5 0.0 3.2179395533040193E-4 1.3791169514160083E-4 24-25 9.194113009440055E-5 4.5970565047200275E-5 0.0 3.677645203776022E-4 1.3791169514160083E-4 26-27 9.194113009440055E-5 4.5970565047200275E-5 0.0 5.976173456136036E-4 1.3791169514160083E-4 28-29 9.194113009440055E-5 4.5970565047200275E-5 0.0 0.001149264126180007 1.3791169514160083E-4 30-31 9.194113009440055E-5 4.5970565047200275E-5 0.0 0.002160616557218413 1.3791169514160083E-4 32-33 9.194113009440055E-5 4.5970565047200275E-5 0.0 0.003470777661063621 1.3791169514160083E-4 34-35 9.194113009440055E-5 4.5970565047200275E-5 0.0 0.004711982917338028 1.3791169514160083E-4 36-37 9.194113009440055E-5 4.5970565047200275E-5 0.0 0.00659677608427324 1.3791169514160083E-4 38-39 9.194113009440055E-5 4.5970565047200275E-5 0.0 0.009492921682246858 1.838822601888011E-4 40-41 9.194113009440055E-5 4.5970565047200275E-5 0.0 0.012894743495739679 2.0686754271240123E-4 42-43 1.149264126180007E-4 4.5970565047200275E-5 0.0 0.015767903811189695 2.298528252360014E-4 44-45 1.838822601888011E-4 4.5970565047200275E-5 0.0 0.017928520368408106 2.298528252360014E-4 46-47 1.838822601888011E-4 4.5970565047200275E-5 0.0 0.022272738765368533 2.298528252360014E-4 48-49 1.838822601888011E-4 9.194113009440055E-5 0.0 0.025467693036148955 2.298528252360014E-4 50-51 1.838822601888011E-4 9.194113009440055E-5 0.0 0.028708617871976573 2.528381077596015E-4 52-53 1.838822601888011E-4 9.194113009440055E-5 0.0 0.03509852641353742 2.7582339028320166E-4 54-55 1.838822601888011E-4 9.194113009440055E-5 0.0 0.04608549145981827 2.7582339028320166E-4 56-57 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.06279579185447559 2.7582339028320166E-4 58-59 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.08467778081694291 2.7582339028320166E-4 60-61 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.11014547385309187 2.7582339028320166E-4 62-63 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.12412052562744075 2.7582339028320166E-4 64-65 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.14140545808518806 2.7582339028320166E-4 66-67 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.18229627569467272 2.7582339028320166E-4 68-69 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.23946067333086624 2.7582339028320166E-4 70-71 1.838822601888011E-4 1.3791169514160083E-4 0.0 0.2958205860787338 2.7582339028320166E-4 72-73 1.838822601888011E-4 1.6089697766520097E-4 0.0 0.3596047450817242 2.988086728068018E-4 74-75 1.838822601888011E-4 1.838822601888011E-4 0.0 0.42258441919638856 3.2179395533040193E-4 76-77 1.838822601888011E-4 1.838822601888011E-4 0.0 0.4742093637443945 3.4477923785400204E-4 78-79 1.838822601888011E-4 1.838822601888011E-4 0.0 0.5243172796458427 3.677645203776022E-4 80-81 1.838822601888011E-4 1.838822601888011E-4 0.0 0.5757813272161835 3.9074980290120236E-4 82-83 1.838822601888011E-4 1.838822601888011E-4 0.0 0.6409675884531134 4.367203679484027E-4 84-85 2.298528252360014E-4 1.838822601888011E-4 0.0 0.7643985556048463 4.597056504720028E-4 86-87 2.298528252360014E-4 1.838822601888011E-4 0.0 0.9412013487763785 4.597056504720028E-4 88-89 2.298528252360014E-4 1.838822601888011E-4 0.0 1.1495399495702903 4.597056504720028E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6110 0.0 32.58184 1 GTATCAA 9210 0.0 31.003994 1 ATCAACG 10820 0.0 25.943184 3 TCAACGC 10955 0.0 25.62348 4 TATCAAC 11250 0.0 25.078236 2 CAACGCA 11260 0.0 25.01378 5 AACGCAG 11670 0.0 24.137197 6 TACATGG 7310 0.0 21.18182 2 ACGCAGA 13190 0.0 21.175589 7 CGCAGAG 13215 0.0 20.957253 8 GTACATG 7325 0.0 20.95066 1 GAGTACT 7025 0.0 20.556547 12-13 AGAGTAC 11025 0.0 19.540318 10-11 ACATGGG 7350 0.0 19.515633 3 AGTACTT 7465 0.0 19.313091 12-13 GCAGAGT 14235 0.0 19.021742 9 GTACTTT 7560 0.0 18.944078 14-15 CAGAGTA 13780 0.0 16.27143 10-11 ACTTTTT 8870 0.0 16.226206 16-17 TACTTTT 8690 0.0 16.207382 14-15 >>END_MODULE