FastQCFastQC Report
Thu 26 May 2016
SRR936995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870588
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150821.7323923601060431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109191.2542097984350806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71340.8194461674178831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23460.2694730458035259No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.2547703391271187No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.19458113367057667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15670.1799932918900789No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11840.1360000367567667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9810.11268246288715213No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.11176354371987668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9360.10751354257122771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61500.052.5520321
GTATCAA114200.043.0754661
GCGTATC459.595238E-442.247711
CGTCACT1353.0559022E-1035.2064251
TATCAAC148450.032.8509452
ATCAACG150150.032.4436153
TCAACGC151300.032.1341974
CAACGCA153850.031.597955
AACGCAG157350.031.0763076
ATCGCGT404.495344E-429.68663824-25
ACGCAGA179350.027.1848377
CGCAGAG179200.027.0515968
GCAGAGT182900.025.9067069
GAGTACT103800.025.15076812-13
TAAACGC950.001222417625.0136534
TACATGG105600.024.7109322
GTACATG106750.024.6659361
AGTACTT104450.023.60694512-13
GTACTTT110400.023.43201614-15
ACATGGG107500.023.2103443
Produced by FastQC (version 0.11.5)