##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936995_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 870588 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.754556690420728 33.0 31.0 34.0 30.0 34.0 2 31.78412750922365 33.0 31.0 34.0 30.0 34.0 3 31.729444926877008 33.0 31.0 34.0 30.0 34.0 4 35.3942266606018 37.0 35.0 37.0 33.0 37.0 5 35.44900917540789 37.0 35.0 37.0 33.0 37.0 6 35.54684994509458 37.0 35.0 37.0 33.0 37.0 7 35.49861243205741 37.0 35.0 37.0 33.0 37.0 8 35.47639870983749 37.0 35.0 37.0 33.0 37.0 9 37.04116068680018 39.0 37.0 39.0 33.0 39.0 10-11 36.91146156390853 39.0 37.0 39.0 33.0 39.0 12-13 36.78647994229188 39.0 37.0 39.0 32.5 39.0 14-15 37.7360835435361 40.0 37.0 41.0 32.5 41.0 16-17 37.71824732249928 40.0 37.0 41.0 32.5 41.0 18-19 37.84257938312956 40.0 37.5 41.0 33.0 41.0 20-21 37.868322903600784 40.0 37.5 41.0 33.0 41.0 22-23 37.71997374188479 40.0 37.0 41.0 32.5 41.0 24-25 37.52041321497654 40.0 37.0 41.0 32.0 41.0 26-27 37.42220832356982 40.0 37.0 41.0 32.0 41.0 28-29 37.40481720400465 40.0 36.5 41.0 32.0 41.0 30-31 37.077914581868804 39.0 36.0 41.0 32.0 41.0 32-33 36.66580517994734 39.0 35.0 41.0 31.0 41.0 34-35 36.35270989262429 39.0 35.0 41.0 30.5 41.0 36-37 36.08757069934343 38.0 35.0 40.5 30.0 41.0 38-39 35.805785285347376 38.0 35.0 40.0 30.0 41.0 40-41 35.451330594954214 38.0 35.0 40.0 28.5 41.0 42-43 34.99398854567258 38.0 35.0 40.0 26.0 41.0 44-45 34.36357898339973 38.0 34.5 40.0 23.0 41.0 46-47 33.487499827702656 38.0 33.0 40.0 13.0 41.0 48-49 32.728489825267516 38.0 33.0 40.0 2.0 41.0 50-51 31.889656186393562 37.0 31.5 39.5 2.0 40.5 52-53 32.06406072677317 37.5 32.5 39.5 2.0 40.5 54-55 32.26571409208489 38.0 33.0 40.0 2.0 41.0 56-57 32.102843135903555 37.0 32.5 40.0 2.0 41.0 58-59 31.83735647631256 37.0 32.0 40.0 2.0 41.0 60-61 31.534848860769962 36.0 31.5 40.0 2.0 41.0 62-63 31.192879984562158 36.0 31.0 39.5 2.0 41.0 64-65 30.87399722945871 35.0 31.0 39.0 2.0 41.0 66-67 30.494868985099725 35.0 30.5 39.0 2.0 41.0 68-69 30.048092208943842 35.0 30.0 38.0 2.0 40.0 70-71 29.71125664493423 35.0 29.0 37.5 2.0 40.0 72-73 29.353790196970323 35.0 29.0 37.0 2.0 39.0 74-75 28.956648839634823 35.0 29.0 36.5 2.0 39.0 76-77 28.56283109806246 34.5 28.5 36.0 2.0 39.0 78-79 28.172996296755755 34.0 27.5 35.5 2.0 37.5 80-81 27.78580281373049 34.0 26.5 35.0 2.0 37.0 82-83 27.398829871305374 34.0 26.0 35.0 2.0 36.5 84-85 27.081099211107897 34.0 25.5 35.0 2.0 36.0 86-87 26.76482733508847 34.0 25.0 35.0 2.0 36.0 88-89 26.41563460557692 34.0 24.0 35.0 2.0 35.5 90-91 26.078357386042534 33.5 23.5 35.0 2.0 35.0 92-93 25.74066780153184 33.0 20.0 35.0 2.0 35.0 94-95 25.34114988949997 33.0 18.5 35.0 2.0 35.0 96-97 24.91974217425464 33.0 8.5 35.0 2.0 35.0 98-99 24.3762945273769 33.0 2.0 35.0 2.0 35.0 100-101 22.514372470100668 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3876391044863752 1101 2 0.40465631102881616 1101 3 0.44667006736580817 1101 4 0.2821551925692489 1101 5 0.21230403158151034 1101 6 0.36287404350626673 1101 7 0.4229167768301565 1101 8 0.2573566624168464 1101 9 0.4006888298548432 1101 10-11 0.32660754984972584 1101 12-13 0.6322129249205801 1101 14-15 0.8535466361108845 1101 16-17 0.754510133235236 1101 18-19 0.9080136935989671 1101 20-21 0.7307466880869384 1101 22-23 0.8098210064853077 1101 24-25 0.7168894763574869 1101 26-27 0.9265626219318435 1101 28-29 0.9770202454952255 1101 30-31 0.7482195644366385 1101 32-33 0.6794620774314524 1101 34-35 0.7787090401362633 1101 36-37 0.8535301483790363 1101 38-39 0.8726432357959979 1101 40-41 1.0872872687628998 1101 42-43 1.360145509288273 1101 44-45 1.1619994511267606 1101 46-47 1.4247101962493076 1101 48-49 1.5032597917346209 1101 50-51 1.449887213816222 1101 52-53 1.5279864487835155 1101 54-55 1.408581484477807 1101 56-57 1.3409729577968363 1101 58-59 1.1849117191493939 1101 60-61 1.3075064388253814 1101 62-63 1.389716070755231 1101 64-65 1.335916950733516 1101 66-67 1.3955628770017583 1101 68-69 1.3698214709933687 1101 70-71 1.4249542461853508 1101 72-73 1.2717278011217061 1101 74-75 1.1660962786189124 1101 76-77 0.9792810478408249 1101 78-79 1.288994205491509 1101 80-81 1.4428600614225964 1101 82-83 1.2576663022313461 1101 84-85 1.191728010304434 1101 86-87 1.4383797215577196 1101 88-89 1.5335737925797268 1101 90-91 1.4066591428860598 1101 92-93 1.3188481073939577 1101 94-95 1.3815786707632718 1101 96-97 1.508237056214302 1101 98-99 1.5564255338477473 1101 100-101 2.0430088340855654 1102 1 0.35799651700796886 1102 2 0.3550563379213756 1102 3 0.36145166871457945 1102 4 0.37667479828288464 1102 5 0.3800438149929306 1102 6 0.2768385246349254 1102 7 0.29276452354357474 1102 8 0.17238779573155938 1102 9 0.4050317203917615 1102 10-11 0.3869748919334839 1102 12-13 0.4914723709337565 1102 14-15 0.5511345799674814 1102 16-17 0.4788704005059543 1102 18-19 0.2862149430688774 1102 20-21 0.3669607580696592 1102 22-23 0.3445440906486894 1102 24-25 0.25416024310586494 1102 26-27 0.4918759978795393 1102 28-29 0.6142518370178607 1102 30-31 0.40011859320133425 1102 32-33 0.5419932366044051 1102 34-35 0.4565374138601612 1102 36-37 0.4884707002971993 1102 38-39 0.5284158281357563 1102 40-41 0.5535474439782959 1102 42-43 0.46178905869302156 1102 44-45 1.0032680302684795 1102 46-47 0.9225549123776062 1102 48-49 1.1598423932799093 1102 50-51 0.8599244755325834 1102 52-53 1.2678674750451435 1102 54-55 1.2951084391017744 1102 56-57 1.4102549263325272 1102 58-59 1.2061406834753718 1102 60-61 1.2332299522354724 1102 62-63 1.2927593264104367 1102 64-65 1.4364518283206316 1102 66-67 1.2871289551349996 1102 68-69 1.328649989523381 1102 70-71 1.0886679024363204 1102 72-73 1.036584003477909 1102 74-75 0.8398351725070619 1102 76-77 1.1307973747302817 1102 78-79 1.273330579753253 1102 80-81 1.1236957478552974 1102 82-83 1.0638484890105495 1102 84-85 1.1836324434402137 1102 86-87 1.4865717137795613 1102 88-89 1.376369946943047 1102 90-91 1.21318007254132 1102 92-93 1.0644095413882262 1102 94-95 1.048104410050204 1102 96-97 1.1055021600092587 1102 98-99 1.3591291415886673 1102 100-101 1.6004360948710392 1103 1 0.3215768283005218 1103 2 0.47031478821290307 1103 3 0.5407577664330248 1103 4 0.2993160172306091 1103 5 0.31330314929967784 1103 6 0.25415211165082496 1103 7 0.19250577526938883 1103 8 0.3131783933854422 1103 9 0.39613817966250053 1103 10-11 0.4002065635168677 1103 12-13 0.20467585832412993 1103 14-15 0.3587669090992591 1103 16-17 0.7220403896792575 1103 18-19 0.6088916716166111 1103 20-21 0.45316652006098224 1103 22-23 0.4764678418530366 1103 24-25 0.794875848457643 1103 26-27 0.8684484771945833 1103 28-29 0.7438351827671426 1103 30-31 0.7202935090212321 1103 32-33 0.8081277559087141 1103 34-35 0.7087834581403882 1103 36-37 0.6796718932211121 1103 38-39 0.6078555214122687 1103 40-41 0.724644119485113 1103 42-43 0.5623532391910473 1103 44-45 0.5987770591429893 1103 46-47 0.6832288994848881 1103 48-49 0.7835070584963901 1103 50-51 0.6379714723640397 1103 52-53 0.9005473572426652 1103 54-55 0.9115644071977655 1103 56-57 1.1537887026667448 1103 58-59 1.1962060658367868 1103 60-61 1.15480712477736 1103 62-63 1.185519092792024 1103 64-65 1.1063559524006088 1103 66-67 0.981890420017745 1103 68-69 0.9945915650570178 1103 70-71 0.9002832664221394 1103 72-73 0.9897330593695237 1103 74-75 0.9647917167028552 1103 76-77 0.9182637275218859 1103 78-79 1.1526860929290557 1103 80-81 1.283897432450587 1103 82-83 1.1921647876686343 1103 84-85 1.3032917251033531 1103 86-87 1.294862087946452 1103 88-89 1.3323939146814574 1103 90-91 1.3250949090978317 1103 92-93 1.270473240147627 1103 94-95 1.25141425241738 1103 96-97 1.3781618787136303 1103 98-99 1.3744311718438418 1103 100-101 1.6495225297071947 1104 1 0.3530553513525163 1104 2 0.2814057539324466 1104 3 0.3839016159765549 1104 4 0.36802140805054506 1104 5 0.36591341176478664 1104 6 0.35068143306430244 1104 7 0.4508745669919634 1104 8 0.39274926891258133 1104 9 0.38864543614833735 1104 10-11 0.3655518846082302 1104 12-13 0.5842785901497507 1104 14-15 0.6828248094020779 1104 16-17 0.6498734389901983 1104 18-19 0.5168506539677438 1104 20-21 0.549820779745481 1104 22-23 0.6201480377751949 1104 24-25 0.4625637325243943 1104 26-27 0.621318208618753 1104 28-29 0.6755543452188704 1104 30-31 0.6267929044621141 1104 32-33 0.6089281898175543 1104 34-35 0.6999004062833052 1104 36-37 0.6673693223349204 1104 38-39 0.6557394460608705 1104 40-41 0.6003246185750228 1104 42-43 0.5841025644651765 1104 44-45 0.470193669656517 1104 46-47 0.10153881034646872 1104 48-49 0.105093431106134 1104 50-51 0.3655557109570573 1104 52-53 0.6209201114624463 1104 54-55 0.5901165917227118 1104 56-57 0.7071095145405053 1104 58-59 0.4553090919482905 1104 60-61 0.5099799836847438 1104 62-63 0.7126543825836826 1104 64-65 0.7941843594554179 1104 66-67 0.5317946734849279 1104 68-69 0.5192423004164404 1104 70-71 0.7203774852737226 1104 72-73 0.6394878606110872 1104 74-75 0.41657383383218516 1104 76-77 0.4996127529441594 1104 78-79 0.7692458021722395 1104 80-81 0.5886189148461334 1104 82-83 0.6339465139957916 1104 84-85 0.7411162833488625 1104 86-87 0.901554634401581 1104 88-89 0.7180167763718295 1104 90-91 0.528788108074167 1104 92-93 0.628951930178733 1104 94-95 0.7062132439109199 1104 96-97 0.4801701281922668 1104 98-99 0.6465277781414365 1104 100-101 0.6783613617060773 1105 1 0.35644931763468435 1105 2 0.42446487689513646 1105 3 0.41111872707728736 1105 4 0.37683147670043127 1105 5 0.2820414648166718 1105 6 0.2787578352498912 1105 7 0.29694587881188284 1105 8 0.3466044036438234 1105 9 0.4511343447552605 1105 10-11 0.4269083036060337 1105 12-13 0.34078690285698343 1105 14-15 0.39817237863572075 1105 16-17 0.5263684420257349 1105 18-19 0.5466193692341719 1105 20-21 0.6536333001769918 1105 22-23 0.6011490651884515 1105 24-25 0.5079205251270196 1105 26-27 0.5523244898497737 1105 28-29 0.6653975479461849 1105 30-31 0.45285067310732785 1105 32-33 0.5418953125934323 1105 34-35 0.5585791294888338 1105 36-37 0.7071448091887049 1105 38-39 0.40393972159442626 1105 40-41 0.504800871318686 1105 42-43 0.2725313226961532 1105 44-45 0.39686379995119836 1105 46-47 0.28498636556992807 1105 48-49 0.1152624872869552 1105 50-51 0.1313796263355691 1105 52-53 0.3825071146847847 1105 54-55 0.3547490579815289 1105 56-57 0.5267208487767121 1105 58-59 0.4061210986731787 1105 60-61 0.27186587076268864 1105 62-63 0.22419293393099693 1105 64-65 0.5115546485321474 1105 66-67 0.6077958176496914 1105 68-69 0.811160761164583 1105 70-71 0.6632859987935475 1105 72-73 0.7621960285464553 1105 74-75 0.8535788074463468 1105 76-77 0.5418627879696096 1105 78-79 0.6923474226831416 1105 80-81 0.6663269660299953 1105 82-83 0.8156649794139987 1105 84-85 0.8073447426959923 1105 86-87 0.8396220467211997 1105 88-89 0.5042097432611037 1105 90-91 0.5718022817070043 1105 92-93 0.5907706851406722 1105 94-95 0.7347671597564336 1105 96-97 1.2352318897389871 1105 98-99 1.3070482563556105 1105 100-101 1.5135187427362915 1106 1 0.5839557114965785 1106 2 0.5784930098874597 1106 3 0.6776148907601538 1106 4 0.45588518514033183 1106 5 0.4201233062488967 1106 6 0.5229010577562789 1106 7 0.5373167228016555 1106 8 0.3472182143855065 1106 9 0.5298939355480954 1106 10-11 0.7593251834014225 1106 12-13 0.6323533990206656 1106 14-15 0.8193168799144885 1106 16-17 0.8757993883172972 1106 18-19 0.7140554306152396 1106 20-21 0.7282816377728949 1106 22-23 0.7230032851372954 1106 24-25 0.7372683512139773 1106 26-27 0.8488142596707533 1106 28-29 0.9300586732658758 1106 30-31 0.6932982434398127 1106 32-33 0.7299132154067323 1106 34-35 0.7176264440668376 1106 36-37 0.6739542721043037 1106 38-39 0.7259410003668023 1106 40-41 0.7607599506025693 1106 42-43 0.9554085605478164 1106 44-45 1.3664993498233216 1106 46-47 0.9009518387157129 1106 48-49 0.9294568606884894 1106 50-51 0.65653947288288 1106 52-53 0.963536526449488 1106 54-55 0.8330482907181036 1106 56-57 0.7499369613086841 1106 58-59 1.1096952674711353 1106 60-61 1.0970640804813563 1106 62-63 0.9504461308619376 1106 64-65 0.9203270270487707 1106 66-67 1.0408968406675925 1106 68-69 0.8374523212668876 1106 70-71 0.9824675844712978 1106 72-73 0.8683341359633339 1106 74-75 0.7861043828939174 1106 76-77 0.9378985935962127 1106 78-79 0.797858931634547 1106 80-81 1.0394535644954637 1106 82-83 1.0670652351684744 1106 84-85 1.188265458808523 1106 86-87 1.0880555505575167 1106 88-89 1.0325729146166012 1106 90-91 1.0037524095842443 1106 92-93 1.3158218360358127 1106 94-95 1.347158464104261 1106 96-97 1.0642024779742805 1106 98-99 1.3464728087852755 1106 100-101 1.360973985703044 1107 1 0.18918084632662868 1107 2 0.36627678011196707 1107 3 0.43543503374272063 1107 4 0.3390684285869625 1107 5 0.27569586363005527 1107 6 0.30669887539080065 1107 7 0.32229351949047924 1107 8 0.37987593418984744 1107 9 0.4218467763859266 1107 10-11 0.5250658080094794 1107 12-13 0.3731510912318825 1107 14-15 0.43662027888601784 1107 16-17 0.46121014963674867 1107 18-19 0.44545013775182696 1107 20-21 0.49718920079811113 1107 22-23 0.5404672259374976 1107 24-25 0.4766073704037481 1107 26-27 0.4802365599341343 1107 28-29 0.42724166586590684 1107 30-31 0.7395267646990646 1107 32-33 0.8196949788583865 1107 34-35 0.8989321006578308 1107 36-37 0.9673156166421251 1107 38-39 1.007996688313625 1107 40-41 1.1359587465388543 1107 42-43 1.191004948356671 1107 44-45 1.2671741271969594 1107 46-47 1.3044461319553946 1107 48-49 1.677443816664919 1107 50-51 1.3856753063207314 1107 52-53 1.4721393108468348 1107 54-55 1.6639939824276198 1107 56-57 1.5318045143000347 1107 58-59 1.631483803781176 1107 60-61 1.4657810000850269 1107 62-63 1.4618007025413569 1107 64-65 1.6165467686767663 1107 66-67 1.5423153782318728 1107 68-69 1.536788831990581 1107 70-71 1.4671471279304704 1107 72-73 1.4842503531964972 1107 74-75 1.4354910629390716 1107 76-77 1.3504471951263746 1107 78-79 1.375635176460836 1107 80-81 1.159277971870356 1107 82-83 1.1954635795808635 1107 84-85 1.290301079026758 1107 86-87 1.4600297133569633 1107 88-89 1.3715268842641635 1107 90-91 1.1867149080164516 1107 92-93 1.2018233411154569 1107 94-95 1.246348229562681 1107 96-97 1.386537859888243 1107 98-99 1.472777838259752 1107 100-101 1.5359971890142212 1108 1 -0.11628907431583713 1108 2 0.1926431285336463 1108 3 0.1492522081074732 1108 4 0.0122833367576618 1108 5 0.02001340241474736 1108 6 -0.02174987267958528 1108 7 -0.2809572874393851 1108 8 -0.1386166800818316 1108 9 -0.05242653404101105 1108 10-11 -0.04999790889281286 1108 12-13 -0.19012443287642355 1108 14-15 0.14076561227367534 1108 16-17 0.21051526192788828 1108 18-19 0.274401377911353 1108 20-21 0.19153254323062185 1108 22-23 0.12755680081107812 1108 24-25 0.2330220667129126 1108 26-27 0.24124837981562308 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18.579922808772096 22-23 21.415220355115565 24.215842025763465 35.372577059341495 18.996360559779475 24-25 20.182194459913713 24.60383825600902 36.0657904643022 19.14817681977507 26-27 19.917582575455445 24.795163220060616 35.91019477452151 19.37705942996243 28-29 19.767008146668775 24.989784335981838 35.76103104929669 19.482176468052703 30-31 20.17653592634242 24.83173578337602 35.640237227692964 19.3514910625886 32-33 19.362086392490347 25.12867278411876 35.51414811635226 19.995092707038634 34-35 19.906653267394205 25.101629344636528 35.56679439643805 19.424922991531226 36-37 20.23965701223454 24.946314783561093 34.906797177625805 19.907231026578565 38-39 20.094733950795348 25.397272333388116 34.6431128544191 19.864880861397438 40-41 20.13756622271061 25.59877744456488 34.334715355852595 19.92894097687191 42-43 19.999057502139298 25.805821303555 33.81101883444048 20.38410235986522 44-45 20.50260271068051 25.994526234254877 33.12030505067802 20.3825660043866 46-47 20.76964974553377 26.266556966767133 32.03424751193044 20.929545775768656 48-49 20.958982191102333 26.793731893176492 30.913382587340827 21.33390332838035 50-51 21.30169118398965 27.455801603287 29.89755692335268 21.34495028937067 52-53 21.499878480471555 27.734950682606506 29.184911353945882 21.58025948297606 54-55 21.376528454537098 27.4972418865496 29.198365817780637 21.927863841132666 56-57 21.74154891821211 28.04908112245514 28.350594550921983 21.85877540841077 58-59 21.676923580865072 27.537242971346345 28.81375899575146 21.97207445203712 60-61 21.7757529750942 27.56923497741922 29.19657946772693 21.45843257975965 62-63 22.055132803426105 27.56640171305127 29.088309379082133 21.2901561044405 64-65 21.873170316887467 27.744646767375492 29.240454907702095 21.14172800803495 66-67 21.931047086808668 28.014728744096978 28.735591227351282 21.318632941743072 68-69 22.050093841190595 27.84566370949112 28.76896218424135 21.335280265076943 70-71 21.781172032935817 28.022098914299203 28.76478053034407 21.431948522420907 72-73 21.644262435396712 28.240303259906124 28.50724688465244 21.608187420044725 74-75 21.125594547655446 28.540585507715978 28.598955882682443 21.734864061946134 76-77 20.899755456267123 28.403214463015058 28.86075716623458 21.83627291448324 78-79 20.82368776163733 28.372463511578133 28.699761609743422 22.10408711704111 80-81 20.629935556229718 28.469885637588828 28.601921821708633 22.29825698447282 82-83 20.790892853448455 28.5367258132366 28.247257028971017 22.425124304343928 84-85 20.755954221373702 28.08061488736375 28.207049779715938 22.95638111154661 86-87 20.453802231654088 28.44298592213062 27.878434336322854 23.22477750989244 88-89 20.662859245164793 27.875441556463183 28.083537165473377 23.37816203289865 90-91 20.342861871918462 27.805386318743498 28.427684811675398 23.424066997662646 92-93 20.330094464609537 27.65913421331701 28.809378062484015 23.201393259589437 94-95 20.10277899928434 27.92134072560364 28.661142582868443 23.31473769224358 96-97 20.037400756638117 27.900297607926706 28.457457357906158 23.604844277529022 98-99 19.81483824222838 27.769375855366818 28.55504824556359 23.86073765684121 100-101 20.188940500245533 27.892558419289436 28.598972091916004 23.319528988549028 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1760.0 1 1233.0 2 774.0 3 849.5 4 751.0 5 772.0 6 1237.5 7 2479.5 8 4046.0 9 4551.0 10 3705.5 11 2908.0 12 2831.0 13 2998.0 14 3150.0 15 3184.0 16 3298.5 17 3458.5 18 3635.5 19 3891.0 20 4195.0 21 4547.0 22 4926.0 23 5445.0 24 5969.5 25 6784.0 26 8357.5 27 10176.5 28 10972.5 29 11771.5 30 13303.5 31 14488.5 32 15646.5 33 16884.5 34 18715.0 35 20429.5 36 21824.0 37 22666.5 38 21603.5 39 20812.5 40 21250.0 41 21825.5 42 22453.5 43 24008.0 44 25890.0 45 26566.0 46 26477.5 47 27016.5 48 29769.0 49 30368.5 50 28221.0 51 26653.5 52 25007.5 53 24011.0 54 23016.5 55 21767.0 56 20912.5 57 20024.0 58 19245.5 59 18451.5 60 17532.5 61 16322.5 62 13698.0 63 9271.0 64 5731.5 65 4111.0 66 2919.0 67 2039.5 68 1459.0 69 1043.5 70 772.0 71 620.0 72 487.5 73 454.0 74 347.5 75 255.0 76 192.0 77 98.5 78 46.5 79 23.5 80 14.0 81 11.0 82 11.5 83 10.0 84 7.0 85 4.5 86 3.0 87 2.5 88 4.0 89 3.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06363515233382495 2 0.10245948715121274 3 0.07179055994339457 4 0.13427706331812522 5 0.1092365160098692 6 0.10441219038167307 7 0.11566895018079734 8 0.10188516267166559 9 0.12014868112126516 10-11 0.11245273309533327 12-13 0.09619935032414874 14-15 0.0956824582925563 16-17 0.11308449002283515 18-19 0.09367232261414124 20-21 0.0770169127072737 22-23 0.07741893984295671 24-25 0.06702366676315318 26-27 0.05800677243426282 28-29 0.0689763699936135 30-31 0.052091230294927106 32-33 0.052206095190836535 34-35 0.04496960674854236 36-37 0.05392906862947801 38-39 0.05364190638970443 40-41 0.06972299181702481 42-43 0.06426690926132683 44-45 0.050253391960376204 46-47 0.0384797401296595 48-49 0.04652028284331969 50-51 0.028831088873267265 52-53 0.0410642002876217 54-55 0.04916217544923661 56-57 0.054847987796753456 58-59 0.06024663790449673 60-61 0.056226366547666634 62-63 0.06449663905314569 64-65 0.044739876956723504 66-67 0.06759799124270034 68-69 0.057547312850625094 70-71 0.026246628715305056 72-73 0.020618248815742923 74-75 0.032564197990323784 76-77 0.045486498780134804 78-79 0.06731082900292676 80-81 0.042729741278308454 82-83 0.06271623316654949 84-85 0.06604731514792302 86-87 0.06955069447316067 88-89 0.09137502469595263 90-91 0.0674831263467909 92-93 0.07873988614591518 94-95 0.08735475333912252 96-97 0.09413178219777897 98-99 0.12382435779036698 100-101 0.12118246518445006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 870588.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.318825345623175 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88694119206015 36.44017147973545 2 13.81877957481279 13.630519526797135 3 5.193528952970498 7.684162420769675 4 2.4325109534388014 4.798743314558542 5 1.2852397897219512 3.169325835827118 6 0.8109962138977053 2.3998428377509544 7 0.4921475292201676 1.6990496626522595 8 0.34175480302132916 1.3483956352989426 9 0.26589667250258253 1.180234039602351 >10 1.303916882874798 11.836774972083335 >50 0.09877310182731178 3.4072924610940873 >100 0.06389701376627906 6.175443962897186 >500 0.0037448799238229166 1.3652281526187227 >1k 0.0011702749761946614 1.0399374674001922 >5k 2.340549952389323E-4 0.8235002655603252 >10k+ 4.681099904778646E-4 3.001377965353801 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15082 1.7323923601060431 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10919 1.2542097984350806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7134 0.8194461674178831 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2346 0.2694730458035259 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2218 0.2547703391271187 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1694 0.19458113367057667 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1567 0.1799932918900789 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1184 0.1360000367567667 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 981 0.11268246288715213 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 973 0.11176354371987668 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 936 0.10751354257122771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.743244795471566E-5 0.0 12-13 0.0 0.0 0.0 1.1486489590943132E-4 0.0 14-15 0.0 0.0 0.0 1.1486489590943132E-4 0.0 16-17 0.0 0.0 0.0 2.2972979181886264E-4 0.0 18-19 0.0 0.0 0.0 2.2972979181886264E-4 0.0 20-21 0.0 0.0 0.0 5.743244795471566E-4 0.0 22-23 0.0 0.0 0.0 0.001206081407049029 0.0 24-25 0.0 0.0 0.0 0.0020101356784150482 0.0 26-27 0.0 0.0 0.0 0.0030439197415999303 0.0 28-29 0.0 0.0 0.0 0.006030407035245145 0.0 30-31 0.0 0.0 0.0 0.014645274228452495 0.0 32-33 0.0 0.0 0.0 0.025729736683712617 0.0 34-35 0.0 0.0 0.0 0.03279392778214264 0.0 36-37 0.0 0.0 0.0 0.03721622627465575 0.0 38-39 0.0 0.0 0.0 0.04221284924671601 0.0 40-41 0.0 0.0 0.0 0.04847298607378002 0.0 42-43 0.0 0.0 0.0 0.05605406920380249 0.0 44-45 0.0 0.0 0.0 0.06352028743791552 0.0 46-47 0.0 0.0 0.0 0.0736858307259002 0.0 48-49 0.0 0.0 0.0 0.0876419155788961 0.0 50-51 0.0 0.0 0.0 0.10630746116417869 0.0 52-53 0.0 0.0 0.0 0.14714193165998152 0.0 54-55 0.0 0.0 0.0 0.19458113367057667 0.0 56-57 0.0 0.0 0.0 0.25109466245801687 0.0 58-59 0.0 0.0 0.0 0.32236833036981904 0.0 60-61 0.0 0.0 0.0 0.4263210611678544 0.0 62-63 0.0 0.0 0.0 0.4928852683473698 0.0 64-65 0.0 0.0 0.0 0.5539933929711873 0.0 66-67 0.0 0.0 0.0 0.6896488350402257 0.0 68-69 0.0 0.0 0.0 0.8603955028095953 0.0 70-71 0.0 0.0 0.0 0.9687705321001439 0.0 72-73 0.0 0.0 0.0 1.0912739435875523 0.0 74-75 0.0 0.0 0.0 1.3442638768280748 0.0 76-77 0.0 0.0 0.0 1.4995037836496712 0.0 78-79 0.0 0.0 0.0 1.6203990865943476 0.0 80-81 0.0 0.0 0.0 1.7787403456054989 0.0 82-83 0.0 0.0 0.0 2.0628012331895222 0.0 84-85 0.0 0.0 0.0 2.363058071096776 0.0 86-87 0.0 0.0 0.0 2.807757515610139 0.0 88-89 0.0 0.0 0.0 3.345095498674459 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6150 0.0 52.552032 1 GTATCAA 11420 0.0 43.075466 1 GCGTATC 45 9.595238E-4 42.24771 1 CGTCACT 135 3.0559022E-10 35.206425 1 TATCAAC 14845 0.0 32.850945 2 ATCAACG 15015 0.0 32.443615 3 TCAACGC 15130 0.0 32.134197 4 CAACGCA 15385 0.0 31.59795 5 AACGCAG 15735 0.0 31.076307 6 ATCGCGT 40 4.495344E-4 29.686638 24-25 ACGCAGA 17935 0.0 27.184837 7 CGCAGAG 17920 0.0 27.051596 8 GCAGAGT 18290 0.0 25.906706 9 GAGTACT 10380 0.0 25.150768 12-13 TAAACGC 95 0.0012224176 25.013653 4 TACATGG 10560 0.0 24.710932 2 GTACATG 10675 0.0 24.665936 1 AGTACTT 10445 0.0 23.606945 12-13 GTACTTT 11040 0.0 23.432016 14-15 ACATGGG 10750 0.0 23.210344 3 >>END_MODULE