FastQCFastQC Report
Thu 26 May 2016
SRR936995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870588
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153621.7645545309606838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119241.369649018824059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80100.920067816234545No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29930.34379063345692795No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.3107095434350117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24550.28199331945765393No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.21801357243610067No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.12704057487583104No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10730.12325003331081982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10620.12198651945581607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10300.11831084278671428No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.11371624695033701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9060.10406759569394479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67400.049.828651
GTATCAA119300.041.9681471
TATCAAC160650.031.2510032
ATCAACG160800.031.10373
TCAACGC162900.030.7592814
CAACGCA165250.030.379345
AACGCAG169350.029.6438456
ACGCAGA190350.026.2985947
CGCAGAG191050.026.0530748
GCAGAGT195150.025.067649
CATGGGT12900.025.0359444
GTACATG96200.024.0476971
TACATGG96500.023.7733022
AGTACTT122600.023.6892212-13
GAGTACT122250.023.50451512-13
ACATGGG98550.022.4112473
GTACTTT130600.021.91207914-15
AGAGTAC184500.021.66219710-11
CATGGGG40200.021.1481194
CAGAGTA191850.021.04272310-11
Produced by FastQC (version 0.11.5)