##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936995_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 870588 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.029051629473415 31.0 31.0 34.0 30.0 34.0 2 31.97271728992359 31.0 31.0 34.0 30.0 34.0 3 32.10958225934656 33.0 31.0 34.0 30.0 34.0 4 35.92935464306882 37.0 35.0 37.0 35.0 37.0 5 35.88191084646239 37.0 35.0 37.0 35.0 37.0 6 35.96744384255239 37.0 35.0 37.0 35.0 37.0 7 35.89645963417828 37.0 35.0 37.0 35.0 37.0 8 35.887116523545004 37.0 35.0 37.0 35.0 37.0 9 37.503417230653305 39.0 37.0 39.0 35.0 39.0 10-11 37.440393159565716 39.0 37.0 39.0 34.5 39.0 12-13 37.32957265664126 39.0 37.0 39.0 34.0 39.0 14-15 38.32499012161895 40.0 38.0 41.0 33.5 41.0 16-17 38.29889741186416 40.0 37.5 41.0 33.5 41.0 18-19 38.50172584506104 40.0 38.0 41.0 34.0 41.0 20-21 38.6256346285499 40.0 38.0 41.0 34.0 41.0 22-23 38.52668828424008 40.0 38.0 41.0 34.0 41.0 24-25 38.390243720336144 40.0 38.0 41.0 34.0 41.0 26-27 38.17217673572344 40.0 37.0 41.0 34.0 41.0 28-29 37.936279847643206 40.0 37.0 41.0 33.0 41.0 30-31 37.54871018208383 39.0 36.5 41.0 33.0 41.0 32-33 37.20768319802249 39.0 35.0 41.0 33.0 41.0 34-35 36.97086681645049 39.0 35.0 41.0 32.5 41.0 36-37 36.67195274917641 39.0 35.0 41.0 31.5 41.0 38-39 36.37682060860017 39.0 35.0 41.0 31.0 41.0 40-41 36.01038436091469 39.0 35.0 41.0 30.5 41.0 42-43 35.513939429443084 38.5 35.0 41.0 29.0 41.0 44-45 34.74479776886426 38.0 35.0 40.0 24.0 41.0 46-47 33.86927341061443 38.0 34.0 40.0 14.5 41.0 48-49 33.065248429796874 38.0 33.0 40.0 2.0 41.0 50-51 32.58932583495293 38.0 33.0 40.0 2.0 41.0 52-53 32.25641118416519 38.0 33.0 40.0 2.0 41.0 54-55 31.961010834056985 37.0 32.0 40.0 2.0 41.0 56-57 31.654785041833797 37.0 31.5 40.0 2.0 41.0 58-59 31.303578156372474 36.0 31.0 40.0 2.0 41.0 60-61 30.964301713324787 36.0 31.0 39.0 2.0 41.0 62-63 30.56808042380552 35.0 30.0 39.0 2.0 40.5 64-65 30.159833928333494 35.0 29.5 39.0 2.0 40.0 66-67 29.782270143856795 35.0 29.0 38.0 2.0 40.0 68-69 29.343861275367914 35.0 29.0 37.5 2.0 40.0 70-71 28.899683891806458 34.5 28.0 37.0 2.0 39.0 72-73 28.5940077281102 34.0 28.0 36.5 2.0 39.0 74-75 28.193914917274302 34.0 27.0 36.0 2.0 39.0 76-77 26.87719621680979 32.5 25.5 35.0 2.0 36.5 78-79 27.529607001245132 34.0 26.0 35.0 2.0 37.0 80-81 27.38011206219245 34.0 26.0 35.0 2.0 37.0 82-83 27.075521371762534 34.0 26.0 35.0 2.0 36.0 84-85 26.726528507169867 34.0 25.0 35.0 2.0 36.0 86-87 26.382230745197496 33.0 24.0 35.0 2.0 35.5 88-89 26.034864367530908 33.0 23.5 35.0 2.0 35.0 90-91 25.63128081250833 33.0 20.5 35.0 2.0 35.0 92-93 25.21556637582875 33.0 18.5 35.0 2.0 35.0 94-95 24.77007780948049 32.0 11.5 35.0 2.0 35.0 96-97 24.20090502051487 32.0 2.0 34.0 2.0 35.0 98-99 23.56426174034101 32.0 2.0 34.0 2.0 35.0 100-101 21.05953102960298 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4406032680387497 1101 2 0.2754182005049195 1101 3 0.30958922853553617 1101 4 0.2563282166466294 1101 5 0.3682391759947734 1101 6 0.3190747963374889 1101 7 0.3984996025612517 1101 8 0.33040048578686054 1101 9 0.48063477004357225 1101 10-11 0.39952434387808466 1101 12-13 0.42175123476622645 1101 14-15 0.5419007911085387 1101 16-17 0.5442727209467151 1101 18-19 0.5275274562007013 1101 20-21 0.6579363108978384 1101 22-23 0.603772699100908 1101 24-25 0.6128621287205149 1101 26-27 0.6982742007833806 1101 28-29 0.5922880437601421 1101 30-31 0.6288877946984925 1101 32-33 0.6461170241814997 1101 34-35 0.5394302218097025 1101 36-37 0.47004824386649346 1101 38-39 0.5245875896827599 1101 40-41 0.4124900367728017 1101 42-43 0.7018683015323361 1101 44-45 0.9395026990129551 1101 46-47 0.7759684068497208 1101 48-49 0.29555260405733463 1101 50-51 0.34806067273781593 1101 52-53 0.34913649398123425 1101 54-55 0.44893188107757 1101 56-57 0.5830972236157557 1101 58-59 0.5210552409983613 1101 60-61 0.5784387094170711 1101 62-63 0.786123788030892 1101 64-65 0.5841784857520373 1101 66-67 0.6672346551642256 1101 68-69 0.3674348926332627 1101 70-71 0.3590607527045826 1101 72-73 0.632199593583735 1101 74-75 0.5653366686456458 1101 76-77 0.4346468549198228 1101 78-79 0.5510556958256885 1101 80-81 0.5614915366957369 1101 82-83 0.36939332821561166 1101 84-85 0.551474788312202 1101 86-87 0.49643066492491883 1101 88-89 0.4723429806052657 1101 90-91 0.6310918721830383 1101 92-93 0.6852413292127615 1101 94-95 0.7546529874548007 1101 96-97 1.1742366320581468 1101 98-99 1.3102033614715118 1101 100-101 1.2178901654604672 1102 1 0.23681581238562188 1102 2 0.14469835896418104 1102 3 0.22382004948277512 1102 4 0.25488791296751856 1102 5 0.2631345018602502 1102 6 0.22450605997770623 1102 7 0.26319251016460754 1102 8 0.22357106739028154 1102 9 0.30166863398788024 1102 10-11 0.3118384231697746 1102 12-13 0.18346436868085192 1102 14-15 0.20376429065860435 1102 16-17 0.27114964092065463 1102 18-19 0.3222318190020772 1102 20-21 0.20561113842341427 1102 22-23 0.23194162577730282 1102 24-25 0.29035887058292786 1102 26-27 0.404067609002162 1102 28-29 0.26179459106518976 1102 30-31 0.3440483484784451 1102 32-33 0.2648334731218327 1102 34-35 0.19681313572247205 1102 36-37 0.19546223420585562 1102 38-39 0.4075945920927424 1102 40-41 0.4930138728936555 1102 42-43 0.3457693782692317 1102 44-45 0.16363347444970344 1102 46-47 0.05435945576315504 1102 48-49 0.18015829183466536 1102 50-51 0.23908760667519857 1102 52-53 0.15092665957736529 1102 54-55 0.11124272808990554 1102 56-57 0.04068196105123789 1102 58-59 0.06360173410455516 1102 60-61 -0.009182565704204393 1102 62-63 0.12206169206580952 1102 64-65 0.22302298467116444 1102 66-67 0.23953492615852312 1102 68-69 0.005326515599509918 1102 70-71 0.15279155296523328 1102 72-73 0.41219191368799457 1102 74-75 0.27051012568477617 1102 76-77 0.3144545472275162 1102 78-79 0.35151674815241307 1102 80-81 0.19292183323797119 1102 82-83 0.2805231003528448 1102 84-85 0.21402288462895314 1102 86-87 0.1829674456781163 1102 88-89 0.29770292723387115 1102 90-91 0.48624309114023134 1102 92-93 0.4521778265597085 1102 94-95 0.4637058261718181 1102 96-97 0.4255941893858548 1102 98-99 0.6235308249855436 1102 100-101 0.6419794384303401 1103 1 0.11550583095987577 1103 2 -0.07699453017633928 1103 3 0.1175619170394171 1103 4 0.143931587292343 1103 5 0.18502455619241687 1103 6 0.2798911032348528 1103 7 0.31149674052506526 1103 8 0.24530269427626195 1103 9 0.3992293976181074 1103 10-11 0.3593697610125801 1103 12-13 0.32673505116046186 1103 14-15 0.26707443100843875 1103 16-17 0.46630407043285516 1103 18-19 0.4204264165764968 1103 20-21 0.25729871353987477 1103 22-23 0.4002124324163603 1103 24-25 0.49295914174714284 1103 26-27 0.467020790960305 1103 28-29 0.5735310105055831 1103 30-31 0.6258884162198441 1103 32-33 0.6567290897969329 1103 34-35 0.6562806655455731 1103 36-37 0.6933788387928033 1103 38-39 0.7593283545211094 1103 40-41 0.7841863849867821 1103 42-43 0.6997429770057906 1103 44-45 0.8839227411143824 1103 46-47 1.1095247368104637 1103 48-49 0.8802257104109046 1103 50-51 0.7615666733935171 1103 52-53 0.8356225186117072 1103 54-55 0.9047171562115182 1103 56-57 0.8539039481982478 1103 58-59 0.9806911636664246 1103 60-61 0.8653524571907987 1103 62-63 0.9089438746894487 1103 64-65 1.0186801357583057 1103 66-67 1.047044958241326 1103 68-69 0.9710485772421897 1103 70-71 0.7164760340284353 1103 72-73 1.073622155947895 1103 74-75 1.035502378135682 1103 76-77 0.9885863002594881 1103 78-79 0.8409992019402672 1103 80-81 1.217018076669941 1103 82-83 1.167953322747234 1103 84-85 1.1117794476969856 1103 86-87 1.0467068052140576 1103 88-89 1.341569699187744 1103 90-91 1.6765211586233661 1103 92-93 1.366960373715461 1103 94-95 1.3464229562015184 1103 96-97 1.4533661730564056 1103 98-99 1.3859833960792365 1103 100-101 1.3866939710445436 1104 1 0.27292805244523066 1104 2 0.18906240614777303 1104 3 0.22335798699064213 1104 4 0.13970855530696014 1104 5 0.17888067230566662 1104 6 0.15734975221989345 1104 7 0.17058768729662432 1104 8 0.1707447448271182 1104 9 0.1898458821275355 1104 10-11 0.27202665920387403 1104 12-13 0.19085313499818568 1104 14-15 0.3940892826753313 1104 16-17 0.3492896095681317 1104 18-19 0.31692838183773375 1104 20-21 0.3029805271461825 1104 22-23 0.16476596461667725 1104 24-25 0.37431574636310216 1104 26-27 0.19765328651641312 1104 28-29 0.3069091874015939 1104 30-31 0.2686650301439997 1104 32-33 0.24551756752773457 1104 34-35 0.12497149010263087 1104 36-37 0.13360150129249604 1104 38-39 0.03659953688716655 1104 40-41 0.3620578631782436 1104 42-43 0.6837378515760619 1104 44-45 0.667732162288992 1104 46-47 0.7946500954898852 1104 48-49 0.7275227212479649 1104 50-51 0.509267157869786 1104 52-53 0.5337817219437113 1104 54-55 0.6293780761124737 1104 56-57 0.568635532237888 1104 58-59 0.6384733968521594 1104 60-61 0.4929227216454457 1104 62-63 0.9479530372240745 1104 64-65 0.7830560306064633 1104 66-67 0.8664611945321603 1104 68-69 0.8024109254658782 1104 70-71 0.6015878922690909 1104 72-73 0.39870561589718534 1104 74-75 0.30063974510010993 1104 76-77 0.3997794666689778 1104 78-79 0.5605056780060025 1104 80-81 0.6192476622893643 1104 82-83 0.6210831152549652 1104 84-85 0.5770679248356494 1104 86-87 0.5257905991215708 1104 88-89 0.6909048025786007 1104 90-91 0.8042110322284728 1104 92-93 0.6398048332656607 1104 94-95 0.5223496606615186 1104 96-97 0.658430140266919 1104 98-99 0.396548673661286 1104 100-101 0.2954144255119431 1105 1 0.10369791187641653 1105 2 -0.07975326657440363 1105 3 0.030625768245016616 1105 4 -0.02133613716957683 1105 5 0.01662059665068938 1105 6 0.005655687866465087 1105 7 0.16931276085007596 1105 8 0.026988615373042535 1105 9 0.1037054534160049 1105 10-11 0.18169251639343287 1105 12-13 0.20510557510466043 1105 14-15 0.4744458417749442 1105 16-17 0.2840021673601356 1105 18-19 0.25755790595859906 1105 20-21 0.13788689243829566 1105 22-23 0.21105443423793702 1105 24-25 0.08877250160525563 1105 26-27 0.2629150433930789 1105 28-29 0.15981162984309805 1105 30-31 0.3262065936801761 1105 32-33 0.3884821217704726 1105 34-35 0.3332310754012795 1105 36-37 0.27469744768020377 1105 38-39 0.447023695108804 1105 40-41 0.4786826938885582 1105 42-43 0.687573138551258 1105 44-45 0.8308632041794297 1105 46-47 0.9390343480467465 1105 48-49 1.0997381978276195 1105 50-51 1.386223329354408 1105 52-53 1.3113163971410131 1105 54-55 1.1280220073691822 1105 56-57 1.2885912834170874 1105 58-59 1.2786820317935543 1105 60-61 1.4591243281461637 1105 62-63 1.199348217721898 1105 64-65 1.1438269408012083 1105 66-67 1.0036751533422326 1105 68-69 0.9921753209265809 1105 70-71 0.8068749842225778 1105 72-73 0.9814339818431073 1105 74-75 0.8186299846741996 1105 76-77 0.9378241124449076 1105 78-79 0.9625429917873518 1105 80-81 1.2125653898380513 1105 82-83 1.2744175382690237 1105 84-85 1.4960587248169688 1105 86-87 1.5019989772096487 1105 88-89 1.4236576863408033 1105 90-91 1.5486647519314545 1105 92-93 1.5068488018828567 1105 94-95 1.6671086855529396 1105 96-97 1.8126682199381463 1105 98-99 1.708509281703133 1105 100-101 2.0215329049403294 1106 1 0.37923499627539314 1106 2 0.330758242377005 1106 3 0.24235211095601272 1106 4 0.2834464715260765 1106 5 0.3439479623795947 1106 6 0.2016019794265631 1106 7 0.2695173644306337 1106 8 0.2901343954241966 1106 9 0.4793448396052611 1106 10-11 0.4245186033499593 1106 12-13 0.582413759247892 1106 14-15 0.6698294734884982 1106 16-17 0.6681965407616701 1106 18-19 0.6491435837079607 1106 20-21 0.562950831057222 1106 22-23 0.6563613396087788 1106 24-25 0.5068095860042305 1106 26-27 0.5756004653879643 1106 28-29 0.6047476912241834 1106 30-31 0.846097898027999 1106 32-33 0.874952710005779 1106 34-35 0.7391965485470564 1106 36-37 0.7363790333579558 1106 38-39 1.005776892039755 1106 40-41 1.0663233588501981 1106 42-43 0.953877486377344 1106 44-45 1.2011573218264857 1106 46-47 0.8778067265633709 1106 48-49 1.0361320597202024 1106 50-51 1.1881734572316418 1106 52-53 0.9291169086499735 1106 54-55 0.9443455367809506 1106 56-57 0.9404646849516034 1106 58-59 0.864660292663114 1106 60-61 0.7662215148469365 1106 62-63 1.0662919517372451 1106 64-65 0.892644076351079 1106 66-67 0.9903311635724101 1106 68-69 0.9078914334585484 1106 70-71 0.8162931427903537 1106 72-73 0.746504314323925 1106 74-75 0.6202796010424869 1106 76-77 0.5781310178157533 1106 78-79 0.7721977232451493 1106 80-81 0.7409093796078743 1106 82-83 0.8660543669135237 1106 84-85 0.6375165253284756 1106 86-87 0.6337636830920026 1106 88-89 0.8764390162640758 1106 90-91 0.9378143682997404 1106 92-93 1.0381595435708384 1106 94-95 1.3722365627907926 1106 96-97 1.800443155999531 1106 98-99 1.6469555732632308 1106 100-101 1.3550239535336779 1107 1 0.241898987244916 1107 2 0.11328928464465449 1107 3 0.12384213980337222 1107 4 0.1572631026561382 1107 5 0.20184586781041958 1107 6 0.23846137990317828 1107 7 0.24145515447202825 1107 8 0.2640563821293256 1107 9 0.29119734180017076 1107 10-11 0.26347382723054835 1107 12-13 0.18118210240604782 1107 14-15 0.4516104844003266 1107 16-17 0.44852891251633764 1107 18-19 0.5001369362728667 1107 20-21 0.3073130535581612 1107 22-23 0.432540945779607 1107 24-25 0.2972560929652204 1107 26-27 0.5110099724743762 1107 28-29 0.41277352842472936 1107 30-31 0.45236655474441534 1107 32-33 0.3545802027939331 1107 34-35 0.31612367760764215 1107 36-37 0.2998005810815201 1107 38-39 0.4066197366402804 1107 40-41 0.5787603336401119 1107 42-43 0.4757324970383294 1107 44-45 0.33166277774019903 1107 46-47 0.21790706520629044 1107 48-49 0.22305793454773948 1107 50-51 0.47853467634615754 1107 52-53 0.23461295781590508 1107 54-55 0.17650281760372621 1107 56-57 0.2351392114786357 1107 58-59 0.33391402400640047 1107 60-61 0.3101510269502832 1107 62-63 0.41897601043535104 1107 64-65 0.42631396221587536 1107 66-67 0.31197797154933227 1107 68-69 0.3935542956183369 1107 70-71 0.43696792016399044 1107 72-73 0.277434955237009 1107 74-75 0.49497419529809505 1107 76-77 0.40414004829361616 1107 78-79 0.48155430169742885 1107 80-81 0.6118851729102701 1107 82-83 0.7396163887371792 1107 84-85 0.6896575011217827 1107 86-87 0.6088220049281965 1107 88-89 0.6867927099803666 1107 90-91 0.8067520329178279 1107 92-93 0.7732354246695472 1107 94-95 0.680641178368365 1107 96-97 1.0918190496405664 1107 98-99 0.745499408707861 1107 100-101 0.7436405344944212 1108 1 -0.007653975873978425 1108 2 -0.14394139543990647 1108 3 -0.13830481423444496 1108 4 -0.04058749874992884 1108 5 -0.05967636633278772 1108 6 -0.07984925074703142 1108 7 -0.13496121681077966 1108 8 -0.06465266589714957 1108 9 0.03193632205811525 1108 10-11 -0.07127757499399223 1108 12-13 0.033090666953647485 1108 14-15 -0.07611032918416782 1108 16-17 -0.01582244262786503 1108 18-19 -0.0571471618896382 1108 20-21 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88.0 7 223.0 8 536.0 9 987.0 10 1841.0 11 3485.0 12 5617.0 13 7494.0 14 9103.0 15 13282.0 16 18982.0 17 26700.0 18 21649.0 19 10046.0 20 6669.0 21 6329.0 22 6366.0 23 6715.0 24 7641.0 25 8581.0 26 9961.0 27 11999.0 28 14608.0 29 17728.0 30 21702.0 31 26578.0 32 33214.0 33 42775.0 34 58258.0 35 81720.0 36 115756.0 37 159131.0 38 107383.0 39 7373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.501427414398215 19.464073386906847 15.949015170227065 18.08548402846787 2 16.472270722293697 22.386624701341663 39.788182319426575 21.35292225693806 3 19.19319896987505 28.95790593332675 31.770647871404385 20.07824722539382 4 12.353762125467359 18.43989960773503 37.40806469213231 31.79827357466531 5 11.741725908849169 40.112270109892386 31.73589071181363 16.410113269444818 6 27.06774517394031 38.247686041356054 17.83660660750345 16.847962177200184 7 25.309331164684103 38.03061838665362 19.61352557122313 17.046524877439158 8 28.206683299103595 31.490670673154238 19.465234990604053 20.837411037138118 9 30.03510271218992 14.850767527234469 20.341079821913468 34.77304993866215 10-11 25.219492836151264 27.895849522132494 25.20662794564354 21.678029696072702 12-13 28.044760886832538 26.80828848304266 26.876461307758092 18.270489322366718 14-15 24.56885461320395 25.574956236474662 29.39484578239075 20.46134336793064 16-17 19.823211438705794 29.012001084324616 29.060072043262714 22.104715433706872 18-19 19.628515440139306 24.76636480172022 36.542830822386705 19.062288935753767 20-21 21.994088916661305 23.143568336993777 36.822109243620254 18.04023350272466 22-23 21.24431993089728 23.726780061291908 36.65011463516612 18.378785372644696 24-25 20.133155822867835 24.098194378554467 37.299195114862975 18.469454683714716 26-27 19.828411566425043 24.248209365916527 37.26540554107273 18.657973526585696 28-29 19.73108052536419 24.504513197282662 37.0087920621027 18.75561421525045 30-31 19.996530484347833 24.234624272708523 36.98348590968805 18.785359333255595 32-33 19.411934301496814 24.453785566683475 36.82836673815133 19.30591339366838 34-35 19.868234315708097 24.499216309492564 36.828973977110145 18.80357539768919 36-37 20.3292941661622 24.293184034626027 36.11627701281591 19.261244786395867 38-39 20.111846390119563 24.73531678706685 35.919840931620584 19.232995891193003 40-41 20.311390782336602 24.97811718163084 35.484914065425606 19.225577970606953 42-43 20.09228484243862 25.336692069694926 34.91517869706529 19.655844390801153 44-45 20.70029216171527 25.383609423378335 34.07988586912511 19.836212545781287 46-47 21.041640822065087 25.913233354927932 32.73890749700203 20.306218326004952 48-49 21.282077494839687 26.536463328765535 31.396058061974273 20.7854011144205 50-51 21.582207417404913 27.103995864861375 30.418079744999783 20.89571697273393 52-53 21.68754551573782 27.38703482054451 29.82007356032429 21.105346103393376 54-55 21.492278259491144 27.356252257881835 29.618183207045107 21.533286275581908 56-57 21.965102795762164 27.74448919721268 28.754728857762966 21.53567914926219 58-59 22.01098926975401 27.24493482192547 29.183592711266616 21.560483197053905 60-61 22.093125433260756 27.244872231510424 29.54862772283028 21.113374612398538 62-63 22.416056734069386 27.260368854153743 29.50494378511994 20.818630626656926 64-65 22.17335994371617 27.38392177696096 29.64641693109538 20.796301348227487 66-67 22.35644185309239 27.70880140778416 29.069950424310925 20.86480631481252 68-69 22.575202047351905 27.590260835205633 29.006602434216873 20.827934683225592 70-71 22.190742787042293 27.78249363072689 29.081446290199935 20.945317292030886 72-73 22.219363360948783 27.966804026016913 28.757936450959843 21.05589616207446 74-75 21.56881438947094 28.36019853408241 28.846712712544843 21.224274363901806 76-77 21.145500700682305 28.40307611017942 29.111913896482804 21.33950929265547 78-79 21.11789327623756 28.266657783740566 28.936208338310838 21.679240601711037 80-81 20.911250515491155 28.357067943744553 28.867791882866623 21.86388965789767 82-83 21.131790470009506 28.36253363466934 28.49319553050166 22.0124803648195 84-85 20.965212956347337 28.052941000788007 28.381467433381502 22.60037860948316 86-87 20.756204835676815 28.197642696575002 28.0832924597764 22.96286000797178 88-89 20.957872670361542 27.801165896103154 28.386411279901214 22.854550153634094 90-91 20.650379141829426 27.864383066778696 28.60296731678545 22.88227047460643 92-93 20.460665929616496 27.691530144873433 29.02218329576292 22.825620629747153 94-95 20.33950675780063 28.02162679986423 28.707486275057104 22.931380167278036 96-97 20.423734848972167 28.11836639986767 28.42259306567666 23.0353056854835 98-99 20.15160382673881 27.823345587918556 28.508825081829652 23.516225503512974 100-101 20.10250661055069 28.45879404257861 28.435016736007572 23.003682610863127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2826.0 1 1901.5 2 997.0 3 967.0 4 795.5 5 811.5 6 1315.0 7 2634.0 8 4173.5 9 4692.0 10 3873.0 11 2961.0 12 2792.0 13 2863.0 14 3004.5 15 3129.5 16 3302.5 17 3582.5 18 3898.5 19 4153.5 20 4443.5 21 4819.0 22 5329.0 23 5864.0 24 6419.5 25 7315.5 26 8800.5 27 10468.0 28 11164.0 29 12198.0 30 13706.5 31 14709.0 32 15780.5 33 16920.0 34 18638.0 35 20039.0 36 21495.0 37 22419.0 38 21205.0 39 20321.5 40 20815.0 41 21739.5 42 22589.5 43 24026.0 44 25860.0 45 26803.0 46 27057.5 47 27438.0 48 29882.0 49 30048.0 50 27602.0 51 25857.5 52 23937.5 53 23099.0 54 22455.0 55 21775.0 56 20954.5 57 19733.5 58 19069.5 59 18574.5 60 17361.0 61 15773.0 62 13192.0 63 8975.0 64 5706.5 65 4147.0 66 2890.5 67 1981.0 68 1498.0 69 1103.0 70 782.0 71 611.5 72 488.0 73 456.0 74 340.0 75 233.0 76 191.5 77 102.0 78 42.5 79 32.0 80 22.5 81 11.5 82 9.0 83 5.0 84 1.5 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015277031155954367 2 0.003216217085464077 3 0.0016081085427320386 4 3.44594687728294E-4 5 0.001033784063184882 6 2.2972979181886264E-4 7 0.0 8 0.0 9 0.0 10-11 1.72297343864147E-4 12-13 7.466218234113036E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0022972979181886265 22-23 0.0 24-25 0.0033310819813735086 26-27 0.0018952707825056168 28-29 0.0034459468772829395 30-31 0.017172301938459984 32-33 5.743244795471566E-4 34-35 0.0031587846375093613 36-37 0.0033310819813735086 38-39 0.002182433022279195 40-41 0.004824325628196116 42-43 0.002584460157962205 44-45 9.763516152301663E-4 46-47 0.0 48-49 1.1486489590943132E-4 50-51 5.743244795471566E-5 52-53 0.0011486489590943132 54-55 0.00304391974159993 56-57 2.871622397735783E-4 58-59 0.0013209463029584603 60-61 0.0010912165111395975 62-63 0.0 64-65 5.743244795471566E-5 66-67 0.0 68-69 0.0 70-71 2.2972979181886264E-4 72-73 5.743244795471566E-5 74-75 8.040542713660193E-4 76-77 9.189191672754506E-4 78-79 4.594595836377253E-4 80-81 0.006777028858656449 82-83 0.002354730366143342 84-85 0.003905406460920665 86-87 0.0019527032304603325 88-89 0.0014932436468226072 90-91 4.0202713568300965E-4 92-93 5.743244795471566E-5 94-95 1.72297343864147E-4 96-97 0.0022972979181886265 98-99 0.0033885144293282245 100-101 0.0011486489590943132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 870588.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.50653827797063 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66765505902774 34.52188736632067 2 14.389926631058298 13.672312006311197 3 5.395173013278173 7.689179798147211 4 2.5000818449305755 4.750809354570152 5 1.411684786175905 3.35321286654472 6 0.8324505536971548 2.372810645623904 7 0.5592819832088796 1.8598685660444374 8 0.4065323459657462 1.5450355563883937 9 0.2756258702381058 1.1784627859378984 >10 1.37815548228471 12.013490141085596 >50 0.10582710475362213 3.493104226321919 >100 0.07079357268656213 6.50464722835188 >500 0.004135706993168814 1.3107068724416495 >1k 0.0019462150556088535 1.6552282715024518 >5k 2.432768819511067E-4 0.9257351246149028 >10k+ 4.865537639022134E-4 3.1535091897930383 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15362 1.7645545309606838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11924 1.369649018824059 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8010 0.920067816234545 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2993 0.34379063345692795 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2705 0.3107095434350117 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2455 0.28199331945765393 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1898 0.21801357243610067 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1106 0.12704057487583104 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1073 0.12325003331081982 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1062 0.12198651945581607 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1030 0.11831084278671428 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 990 0.11371624695033701 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 906 0.10406759569394479 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1486489590943132E-4 0.0 10-11 0.0 0.0 0.0 1.7229734386414698E-4 0.0 12-13 0.0 0.0 0.0 2.2972979181886264E-4 0.0 14-15 0.0 0.0 0.0 2.2972979181886264E-4 0.0 16-17 0.0 0.0 0.0 4.594595836377253E-4 0.0 18-19 0.0 0.0 0.0 4.594595836377253E-4 0.0 20-21 0.0 0.0 0.0 8.040542713660193E-4 0.0 22-23 0.0 0.0 0.0 0.0014358111988678917 0.0 24-25 0.0 0.0 0.0 0.0022398654702339106 0.0 26-27 0.0 0.0 0.0 0.003216217085464077 0.0 28-29 0.0 0.0 0.0 0.006317569275018723 0.0 30-31 0.0 0.0 0.0 0.014989868916180787 0.0 32-33 0.0 0.0 0.0 0.02618919626735034 0.0 34-35 0.0 0.0 0.0 0.03359798205350867 0.0 36-37 0.0 0.0 0.0 0.03836487523375006 0.0 38-39 0.0 0.0 0.0 0.043418930653765044 0.0 40-41 0.0 0.0 0.0 0.04962163503287433 0.0 42-43 0.0 0.0 0.0 0.05726015061085152 0.0 44-45 0.0 0.0 0.0 0.06530069332451172 0.0 46-47 0.0 0.0 0.0 0.07563853395636053 0.0 48-49 0.0 0.0 0.0 0.08970948370526587 0.0 50-51 0.0 0.0 0.0 0.10837502929054846 0.0 52-53 0.0 0.0 0.0 0.149266932234306 0.0 54-55 0.0 0.0 0.0 0.196131809765354 0.0 56-57 0.0 0.0 0.0 0.25356425772006963 0.0 58-59 0.0 0.0 0.0 0.3254696825593737 0.0 60-61 0.0 0.0 0.0 0.4312028192440052 0.0 62-63 0.0 0.0 0.0 0.49948999986216214 0.0 64-65 0.0 0.0 0.0 0.5612298814134815 0.0 66-67 0.0 0.0 0.0 0.6983211346813878 0.0 68-69 0.0 0.0 0.0 0.8712502354730367 0.0 70-71 0.0 0.0 0.0 0.9812908057542717 0.0 72-73 0.0 0.0 0.0 1.1035070550019066 0.0 74-75 0.0 0.0 0.0 1.3618956383501724 0.0 76-77 0.0 0.0 0.0 1.5194902755379123 0.0 78-79 0.0 0.0 0.0 1.642338281713049 0.0 80-81 0.0 0.0 0.0 1.8021153519230682 0.0 82-83 0.0 0.0 0.0 2.090368808207786 0.0 84-85 0.0 0.0 0.0 2.3914297003864053 0.0 86-87 0.0 0.0 0.0 2.8363588746915878 0.0 88-89 0.0 0.0 0.0 3.379152940311606 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6740 0.0 49.82865 1 GTATCAA 11930 0.0 41.968147 1 TATCAAC 16065 0.0 31.251003 2 ATCAACG 16080 0.0 31.1037 3 TCAACGC 16290 0.0 30.759281 4 CAACGCA 16525 0.0 30.37934 5 AACGCAG 16935 0.0 29.643845 6 ACGCAGA 19035 0.0 26.298594 7 CGCAGAG 19105 0.0 26.053074 8 GCAGAGT 19515 0.0 25.06764 9 CATGGGT 1290 0.0 25.035944 4 GTACATG 9620 0.0 24.047697 1 TACATGG 9650 0.0 23.773302 2 AGTACTT 12260 0.0 23.68922 12-13 GAGTACT 12225 0.0 23.504515 12-13 ACATGGG 9855 0.0 22.411247 3 GTACTTT 13060 0.0 21.912079 14-15 AGAGTAC 18450 0.0 21.662197 10-11 CATGGGG 4020 0.0 21.148119 4 CAGAGTA 19185 0.0 21.042723 10-11 >>END_MODULE