FastQCFastQC Report
Thu 26 May 2016
SRR936994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795677
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137241.7248204987702294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101321.2733810327557538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66160.8314931812783328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.2791333669315564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20690.26003013785744716No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.20435427943751044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.1799725265402921No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10460.13146037902314633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10050.12630753433868266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9350.11750999463350077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.11650456152433714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT352.7782924E-454.321774
GGTATCA55000.047.7043951
GTATCAA102500.043.6826551
TATCAAC135300.033.0929222
ATCAACG134850.033.0229573
TCAACGC135600.032.8794984
CAACGCA137900.032.302745
CGTCACT1805.456968E-1231.6877021
AACGCAG141250.031.5682896
ACGCAGA157150.028.0113017
CGCAGAG157300.027.8939258
GCAGAGT160200.027.0922419
GTACATG89550.027.0168191
TACATGG88700.026.8470232
TCTCGCA908.910497E-426.4064182
CATGGGT10700.026.2089864
ACATGGG91250.025.2080843
AGTACTT96000.024.60283712-13
GAGTACT95750.024.5678112-13
CATGGGA44700.023.3936044
Produced by FastQC (version 0.11.5)