##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936994_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 795677 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84828894136691 33.0 31.0 34.0 30.0 34.0 2 31.908471653698673 33.0 31.0 34.0 30.0 34.0 3 31.859273298084524 33.0 31.0 34.0 30.0 34.0 4 35.53151215882827 37.0 35.0 37.0 33.0 37.0 5 35.556110079843954 37.0 35.0 37.0 33.0 37.0 6 35.67115676336001 37.0 35.0 37.0 33.0 37.0 7 35.62795832982479 37.0 35.0 37.0 33.0 37.0 8 35.57956809107213 37.0 35.0 37.0 33.0 37.0 9 37.18512160085059 39.0 37.0 39.0 34.0 39.0 10-11 37.01209158992908 39.0 37.0 39.0 33.0 39.0 12-13 36.926006407122486 39.0 37.0 39.0 33.0 39.0 14-15 37.91728616008757 40.0 37.5 41.0 33.0 41.0 16-17 37.89866428211448 40.0 37.5 41.0 33.0 41.0 18-19 38.03121869803953 40.0 38.0 41.0 33.0 41.0 20-21 38.03948084461408 40.0 38.0 41.0 33.0 41.0 22-23 37.92409859779785 40.0 37.5 41.0 33.0 41.0 24-25 37.7489188452098 40.0 37.0 41.0 33.0 41.0 26-27 37.73556543672872 40.0 37.0 41.0 33.0 41.0 28-29 37.63321360300725 40.0 37.0 41.0 33.0 41.0 30-31 37.290468368445985 39.0 36.5 41.0 32.0 41.0 32-33 36.86346029858849 39.0 35.0 41.0 31.5 41.0 34-35 36.578369740485144 39.0 35.0 41.0 31.0 41.0 36-37 36.290614784642514 39.0 35.0 41.0 30.5 41.0 38-39 36.01496272985143 38.0 35.0 40.5 30.0 41.0 40-41 35.68824975461148 38.0 35.0 40.0 29.5 41.0 42-43 35.230672747861256 38.0 35.0 40.0 27.5 41.0 44-45 34.578545062883556 38.0 34.5 40.0 24.0 41.0 46-47 33.67996435739628 38.0 33.5 40.0 14.0 41.0 48-49 32.921042081146 38.0 33.0 40.0 3.5 41.0 50-51 32.03601649915732 37.5 32.0 39.5 2.0 40.5 52-53 32.155446871029326 37.5 32.5 39.5 2.0 40.5 54-55 32.42996404319844 38.0 33.0 40.0 2.0 41.0 56-57 32.27636528390289 37.5 33.0 40.0 2.0 41.0 58-59 32.01453102201019 37.0 32.5 40.0 2.0 41.0 60-61 31.71467819228154 36.5 32.0 40.0 2.0 41.0 62-63 31.374959311378866 36.0 31.5 40.0 2.0 41.0 64-65 31.048887928141696 35.5 31.0 39.0 2.0 41.0 66-67 30.670361842808074 35.0 31.0 39.0 2.0 41.0 68-69 30.27244095279869 35.0 30.5 38.5 2.0 40.0 70-71 29.872845388266846 35.0 30.0 37.5 2.0 40.0 72-73 29.492950657113376 35.0 29.5 37.0 2.0 39.5 74-75 29.080564098245894 35.0 29.0 36.5 2.0 39.0 76-77 28.688756869935915 35.0 29.0 36.0 2.0 39.0 78-79 28.2878045991024 34.0 28.0 35.5 2.0 37.5 80-81 27.904250091431575 34.0 27.0 35.0 2.0 37.0 82-83 27.531252631406964 34.0 26.0 35.0 2.0 37.0 84-85 27.208210115411156 34.0 26.0 35.0 2.0 36.0 86-87 26.893315377973725 34.0 25.0 35.0 2.0 36.0 88-89 26.563161936313353 34.0 24.5 35.0 2.0 35.5 90-91 26.216286885256203 34.0 24.0 35.0 2.0 35.0 92-93 25.879572364162847 33.0 21.5 35.0 2.0 35.0 94-95 25.47820221019333 33.0 19.0 35.0 2.0 35.0 96-97 25.036762404845184 33.0 12.0 35.0 2.0 35.0 98-99 24.507773254725223 33.0 2.0 35.0 2.0 35.0 100-101 22.60442679630051 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3592563439204959 1101 2 0.4576102822989121 1101 3 0.3066432803708352 1101 4 0.2847611631142897 1101 5 0.33264527110654285 1101 6 0.1861643795645378 1101 7 0.20296061485141337 1101 8 0.2858423497295064 1101 9 0.1896454542612247 1101 10-11 0.4612113389466188 1101 12-13 0.3056918538726521 1101 14-15 0.5980068019369682 1101 16-17 0.7157842300116926 1101 18-19 0.5549693919690597 1101 20-21 0.6825291115722294 1101 22-23 0.8505255088936607 1101 24-25 0.6534684282943459 1101 26-27 0.7512535676094814 1101 28-29 0.730275756243266 1101 30-31 0.5972929982926942 1101 32-33 0.5654693359882472 1101 34-35 0.34271834701822 1101 36-37 0.3507178825340844 1101 38-39 0.44259318223902966 1101 40-41 0.5600969697954454 1101 42-43 0.8338316529605052 1101 44-45 0.692555482676191 1101 46-47 0.9738267730322931 1101 48-49 1.1571253470161764 1101 50-51 1.0345282031443865 1101 52-53 1.0181319748880924 1101 54-55 1.0663578039383097 1101 56-57 1.0499842280068634 1101 58-59 0.8902760229841711 1101 60-61 1.2051499377919157 1101 62-63 1.3132738539432793 1101 64-65 1.1590392756736598 1101 66-67 1.1660196215000624 1101 68-69 1.1525323211887972 1101 70-71 1.1046127160046915 1101 72-73 1.1494116517063908 1101 74-75 0.9926510700784128 1101 76-77 0.9763525427925295 1101 78-79 1.0626073576276553 1101 80-81 0.9067053375886793 1101 82-83 1.1450235487535956 1101 84-85 1.2839716354235016 1101 86-87 1.1653410610256607 1101 88-89 1.2219961205908696 1101 90-91 1.33001616972075 1101 92-93 1.3970972105851907 1101 94-95 1.2886888778302286 1101 96-97 1.1703850762963306 1101 98-99 1.4125570704092247 1101 100-101 1.194574580012592 1102 1 0.1307692839246002 1102 2 -0.0028638425777423038 1102 3 0.08092838055939211 1102 4 0.1457550374951282 1102 5 -0.08325202951774457 1102 6 -0.12955657747797034 1102 7 0.07325038496972525 1102 8 0.08101024249697275 1102 9 0.04213478083390498 1102 10-11 0.28554064715221017 1102 12-13 0.1435424649691086 1102 14-15 0.08234470750277723 1102 16-17 0.3009139719931895 1102 18-19 0.011104536609870763 1102 20-21 0.0647513826430881 1102 22-23 0.25725636267014806 1102 24-25 0.17384075261021792 1102 26-27 0.12973159060742034 1102 28-29 0.20683329505350656 1102 30-31 0.27184261385391295 1102 32-33 0.2910205928636955 1102 34-35 0.2551632701793096 1102 36-37 -0.05440096620122148 1102 38-39 0.2887785921279473 1102 40-41 0.5557042560582417 1102 42-43 0.43943263774904295 1102 44-45 0.5141773551421593 1102 46-47 0.5068496732736065 1102 48-49 0.6510449128494855 1102 50-51 0.5850035003883427 1102 52-53 0.7091594571316193 1102 54-55 0.5560751657210901 1102 56-57 0.7083710505983802 1102 58-59 0.7039954071005461 1102 60-61 0.6406990453273096 1102 62-63 0.6803750292415032 1102 64-65 0.6908079871443 1102 66-67 0.34656296489621496 1102 68-69 0.1569360258694772 1102 70-71 0.5338469159128572 1102 72-73 0.4949595868744794 1102 74-75 0.5507088970956175 1102 76-77 0.6447313542074831 1102 78-79 0.7623506580370041 1102 80-81 0.9325963367387153 1102 82-83 0.6719965452267225 1102 84-85 0.7444628573233238 1102 86-87 0.7755338177496753 1102 88-89 0.6733807352239687 1102 90-91 0.758863244175842 1102 92-93 1.0888574223928842 1102 94-95 1.043466274588635 1102 96-97 1.2170868162848016 1102 98-99 0.9388742739224192 1102 100-101 1.0270152044935763 1103 1 0.3967142584056198 1103 2 0.4296050514126044 1103 3 0.4207040569118021 1103 4 0.24189173778928108 1103 5 0.2099915837170201 1103 6 0.268067969416812 1103 7 0.28930756807688596 1103 8 0.27719163903219624 1103 9 0.1336602799224309 1103 10-11 0.2899991557018282 1103 12-13 0.13031085653626207 1103 14-15 0.2002444989983232 1103 16-17 0.49583112013899466 1103 18-19 0.25969344585778487 1103 20-21 0.524172578021826 1103 22-23 0.46472356007542714 1103 24-25 0.566521801638018 1103 26-27 0.6542226735903185 1103 28-29 0.5624129538176064 1103 30-31 0.48354951883812447 1103 32-33 0.4782878974463429 1103 34-35 0.7945837167204957 1103 36-37 0.7899244832768133 1103 38-39 0.9068223008395009 1103 40-41 0.9126224619370831 1103 42-43 0.8675723146724437 1103 44-45 0.8599811737456236 1103 46-47 0.6284696663000489 1103 48-49 0.9974875834849897 1103 50-51 1.1862516019681628 1103 52-53 1.3428310544255027 1103 54-55 1.0272501854365785 1103 56-57 1.0745262846218182 1103 58-59 1.0473561432078533 1103 60-61 1.1170738057577978 1103 62-63 1.1556458393199165 1103 64-65 1.1340613118660023 1103 66-67 1.2300455977154243 1103 68-69 1.1574344708615065 1103 70-71 1.3925348355251437 1103 72-73 1.3747816911547872 1103 74-75 1.1757290806076206 1103 76-77 1.207134093080807 1103 78-79 1.2213576526477148 1103 80-81 1.1856010473949077 1103 82-83 1.0818031579302847 1103 84-85 1.095031154817761 1103 86-87 1.3347668676947997 1103 88-89 1.4137862541566868 1103 90-91 1.5255289747955771 1103 92-93 1.6608499620988262 1103 94-95 1.4901695172892246 1103 96-97 1.3243145026809948 1103 98-99 1.4573892822649377 1103 100-101 1.461514150270716 1104 1 0.35387134526195396 1104 2 0.32753669370514515 1104 3 0.32328215967788054 1104 4 0.20156197565501088 1104 5 0.10767305846616892 1104 6 0.2172047766363221 1104 7 0.26421737005435375 1104 8 0.22142837556216932 1104 9 0.10958911299412222 1104 10-11 0.3564410158466913 1104 12-13 0.24136172087659702 1104 14-15 0.3991810574273629 1104 16-17 0.48861298322160707 1104 18-19 0.5003265074494934 1104 20-21 0.5507786995527582 1104 22-23 0.4858259956515525 1104 24-25 0.49937075093433236 1104 26-27 0.25119128559652637 1104 28-29 0.16967056671430925 1104 30-31 0.417288399005578 1104 32-33 0.47895003814608117 1104 34-35 0.3394916596547546 1104 36-37 0.3727609241959655 1104 38-39 0.41827205618023555 1104 40-41 0.17049183775758792 1104 42-43 0.1024228338274682 1104 44-45 0.24806791991507993 1104 46-47 0.23006695163793722 1104 48-49 -0.02497528155306128 1104 50-51 -0.02411749843853528 1104 52-53 0.2703501727340978 1104 54-55 0.24763918449098554 1104 56-57 0.24375315215694826 1104 58-59 0.35587239721283126 1104 60-61 0.40122569188336854 1104 62-63 0.5023332997291838 1104 64-65 0.37473625930786625 1104 66-67 0.11430638705140339 1104 68-69 0.11036739841849652 1104 70-71 0.23103562045450232 1104 72-73 0.3499118241796566 1104 74-75 0.30482528724141744 1104 76-77 0.21201809796481186 1104 78-79 0.41636440028604227 1104 80-81 0.5302584785185012 1104 82-83 0.5188521678176379 1104 84-85 0.40770443600608175 1104 86-87 0.35727506125562414 1104 88-89 0.5658623004996493 1104 90-91 0.702385952404434 1104 92-93 0.4486906719320167 1104 94-95 0.5925173052573136 1104 96-97 0.4379054856328821 1104 98-99 0.425624315819519 1104 100-101 0.6267303042086745 1105 1 0.1754444916467257 1105 2 0.3000188222690845 1105 3 0.3243367354095241 1105 4 0.26395789811552106 1105 5 0.34724460401187685 1105 6 0.24529421201854973 1105 7 0.24925780504096906 1105 8 0.36295887890644707 1105 9 0.4432536544089274 1105 10-11 0.47678512724301214 1105 12-13 0.35799929183333745 1105 14-15 0.5981111863225124 1105 16-17 0.5100426061044132 1105 18-19 0.4093242119934857 1105 20-21 0.43648546079773354 1105 22-23 0.508533299709768 1105 24-25 0.4314748192736033 1105 26-27 0.41492768076457054 1105 28-29 0.4501877113326387 1105 30-31 0.1664431283809833 1105 32-33 0.4395926278252702 1105 34-35 0.4163129590944692 1105 36-37 0.4180041781313619 1105 38-39 0.2730367374384528 1105 40-41 0.4299030923921947 1105 42-43 0.7067240537772435 1105 44-45 0.5221989587124298 1105 46-47 0.789785033759145 1105 48-49 0.8286071708587741 1105 50-51 0.5852421199361508 1105 52-53 0.3877576874774604 1105 54-55 0.5769663950749262 1105 56-57 0.7628378678240111 1105 58-59 0.7017065355658083 1105 60-61 0.7165501608004092 1105 62-63 0.6554307853078285 1105 64-65 0.5464308486558451 1105 66-67 0.5567762333156523 1105 68-69 0.7065430951054985 1105 70-71 0.5957267689185244 1105 72-73 0.8290457150465933 1105 74-75 0.5586853564271088 1105 76-77 0.6119472562362738 1105 78-79 0.47947334014417464 1105 80-81 0.570380657499669 1105 82-83 0.7830613395214279 1105 84-85 0.46245324687706457 1105 86-87 0.5846839100315329 1105 88-89 0.756803184457219 1105 90-91 0.7286341928615023 1105 92-93 0.5880108213436621 1105 94-95 0.7293309685106983 1105 96-97 0.6843484488411846 1105 98-99 0.6701910082468139 1105 100-101 0.611076122246633 1106 1 0.33823708952869325 1106 2 0.36993324105219116 1106 3 0.33954051628276716 1106 4 0.3799490451311911 1106 5 0.2865467150625989 1106 6 0.3233759706788035 1106 7 0.36932018802846045 1106 8 0.25569045561632464 1106 9 0.3797930564653882 1106 10-11 0.4509076740509812 1106 12-13 0.38690944872631405 1106 14-15 0.5834124613369838 1106 16-17 0.6225936889844945 1106 18-19 0.6613180873767206 1106 20-21 0.5411421589756245 1106 22-23 0.4594339333683948 1106 24-25 0.6759331187095796 1106 26-27 0.6103772041883886 1106 28-29 0.41573555782880334 1106 30-31 0.6925591953159795 1106 32-33 0.7011405264256751 1106 34-35 0.7719776967200005 1106 36-37 0.5334298757064957 1106 38-39 0.5942209010647233 1106 40-41 0.46607764633844084 1106 42-43 0.5076071022543189 1106 44-45 0.8673558102425787 1106 46-47 0.5992966630706249 1106 48-49 0.7677967841150419 1106 50-51 0.8334288331176971 1106 52-53 0.6531778785816016 1106 54-55 0.7148738887306436 1106 56-57 0.6205725648674516 1106 58-59 0.8078695200020327 1106 60-61 0.8910549632720404 1106 62-63 0.9161544895404745 1106 64-65 0.8199846302988085 1106 66-67 0.8580548003542745 1106 68-69 1.0318470463832945 1106 70-71 0.8550412902522488 1106 72-73 1.0916398431354004 1106 74-75 1.0254872572845777 1106 76-77 1.2965573211178985 1106 78-79 1.0119893473961383 1106 80-81 1.0426558721040138 1106 82-83 0.9994680178641815 1106 84-85 1.2597026165933691 1106 86-87 1.2758034525562927 1106 88-89 1.2556894562921492 1106 90-91 1.2257844460999543 1106 92-93 1.386545034291327 1106 94-95 1.1876292202949692 1106 96-97 1.471384795102292 1106 98-99 1.434419906917089 1106 100-101 1.4481739870384978 1107 1 0.21367938785688168 1107 2 0.22951231371355263 1107 3 0.29589320073154113 1107 4 0.2364225613352673 1107 5 0.35213852014474156 1107 6 0.21860942845081155 1107 7 0.1924224982594822 1107 8 0.21972948246983037 1107 9 0.23300231181715247 1107 10-11 0.4721776699030258 1107 12-13 0.37914195066503 1107 14-15 0.4204032095921377 1107 16-17 0.28776528811162194 1107 18-19 0.3717763463173682 1107 20-21 0.5338002772464634 1107 22-23 0.48176616659171856 1107 24-25 0.546118333952414 1107 26-27 0.31549001055099524 1107 28-29 0.30114737769931565 1107 30-31 0.3444952085894428 1107 32-33 0.4645357184038872 1107 34-35 0.25982057936892033 1107 36-37 0.33351824519917983 1107 38-39 0.048030708274950484 1107 40-41 0.13877508001623085 1107 42-43 0.14907583929195312 1107 44-45 0.20818071240491776 1107 46-47 0.1917437766344392 1107 48-49 0.09845017732321537 1107 50-51 0.09094835556173919 1107 52-53 0.2509127340343582 1107 54-55 0.11576479749364665 1107 56-57 0.22975217135269332 1107 58-59 0.2491398765458861 1107 60-61 0.133804156224123 1107 62-63 0.13368050443704504 1107 64-65 0.1720180303601282 1107 66-67 0.13525006086393176 1107 68-69 0.07434736198862524 1107 70-71 -0.035200192912459016 1107 72-73 0.0338966917710799 1107 74-75 0.348549891416841 1107 76-77 0.23835969807690205 1107 78-79 0.4058557832200478 1107 80-81 0.42459783679228735 1107 82-83 0.46349802742251356 1107 84-85 0.4247371528338775 1107 86-87 0.09966682249984515 1107 88-89 0.197176227920135 1107 90-91 0.2839748553048125 1107 92-93 0.3861608975169979 1107 94-95 0.38792679103564964 1107 96-97 0.5339017025307378 1107 98-99 0.7956870172346804 1107 100-101 0.6238579488773048 1108 1 0.05647686504809357 1108 2 0.039809369634209446 1108 3 -0.011609025984231636 1108 4 0.02630123541629814 1108 5 -0.0781773124062326 1108 6 0.07885713553537954 1108 7 -0.050308779273557036 1108 8 0.018705193213435223 1108 9 0.13040126706618338 1108 10-11 0.1540166964069627 1108 12-13 0.16308552448366243 1108 14-15 0.331146210482828 1108 16-17 0.33567518435869914 1108 18-19 0.17800874322644233 1108 20-21 0.08358302200466028 1108 22-23 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2316 72-73 -1.479773395749465 2316 74-75 -1.639032853263064 2316 76-77 -1.924108647918029 2316 78-79 -1.8515634713003664 2316 80-81 -2.058077981769177 2316 82-83 -1.4859555244900555 2316 84-85 -1.847598357406472 2316 86-87 -2.644653227258029 2316 88-89 -2.4778759734215505 2316 90-91 -2.0478746271244397 2316 92-93 -1.9796238996752393 2316 94-95 -2.414010211940937 2316 96-97 -2.668228556476773 2316 98-99 -2.6085246314391846 2316 100-101 -2.4815665479906173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1225.0 3 387.0 4 419.0 5 487.0 6 602.0 7 777.0 8 965.0 9 1296.0 10 1901.0 11 2940.0 12 4750.0 13 6768.0 14 8036.0 15 11048.0 16 15466.0 17 22148.0 18 17828.0 19 8268.0 20 6450.0 21 5843.0 22 6193.0 23 6610.0 24 7102.0 25 7936.0 26 9025.0 27 10455.0 28 12284.0 29 14508.0 30 17890.0 31 21924.0 32 27650.0 33 36296.0 34 50361.0 35 72921.0 36 102926.0 37 139328.0 38 116238.0 39 18422.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.004235748510446 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19.581504153336155 25.16306237265418 35.83757321696993 19.417860257039738 20-21 22.188340153060327 23.512402513951727 35.802183486284676 18.49707384670327 22-23 21.263427849469803 24.09155766196705 35.63080249668429 19.014211991878863 24-25 19.997875708856473 24.521861658367474 36.31940791355015 19.160854719225902 26-27 19.833255927515474 24.702343730851485 36.17539425118827 19.289006090444772 28-29 19.683205876585667 24.850546279117708 36.08050389409275 19.385743950203878 30-31 20.02580471624239 24.753108066833335 35.90739462864713 19.313692588277142 32-33 19.23866825704263 25.06859439002839 35.7943940504186 19.898343302510373 34-35 19.771998127217252 25.0768752179938 35.83192717514604 19.319199479642915 36-37 20.124566465088776 24.872068317372378 35.115840779649574 19.88752443788927 38-39 20.022561021593223 25.347087805736408 34.904225635353555 19.72612553731681 40-41 20.13701186929261 25.50518492077607 34.46148277233795 19.896320437593364 42-43 19.850109596203346 25.766167953667953 34.08284819417632 20.30087425595238 44-45 20.270225263127468 25.952798240978197 33.3674974345504 20.409479061343937 46-47 20.60360180328961 26.18609526466807 32.28438887611119 20.925914055931134 48-49 20.795215610472166 26.762003390848733 31.039273770188935 21.403507228490167 50-51 21.188454304310312 27.410312597995194 30.00432985583277 21.396903241861725 52-53 21.359619879053568 27.686024421803033 29.32878689711384 21.62556880202956 54-55 21.25159310121189 27.51873386474414 29.301750337473507 21.927922696570466 56-57 21.57065351294003 28.00855042513287 28.4755429180557 21.945253143871398 58-59 21.526146010933633 27.500177534836105 28.93396772448102 22.039708729749247 60-61 21.67108933368363 27.488080168643776 29.318951180544122 21.52187931712848 62-63 22.00839391836858 27.473490904703006 29.270993594578016 21.2471215823504 64-65 21.742267224898725 27.68138038001174 29.449087300639636 21.1272650944499 66-67 21.85056368885934 27.924941205159115 28.893877669021812 21.330617436959738 68-69 21.994542989514017 27.82886784945892 28.909647569644136 21.266941591382928 70-71 21.704761791031267 27.857385731910973 29.050803206162428 21.387049270895332 72-73 21.608983589124108 28.114883044776533 28.712402086885692 21.563731279213666 74-75 21.08987835421518 28.48516273384053 28.785019122051164 21.639939789893123 76-77 20.772683903068184 28.2848116939686 29.11688060269998 21.825623800263237 78-79 20.60895715286098 28.343468580315285 28.928136342435153 22.119437924388585 80-81 20.519285250481023 28.386696914095914 28.792196012393152 22.30182182302991 82-83 20.754271045020797 28.355879455926157 28.413897404403958 22.475952094649085 84-85 20.6496270354662 28.04022033442361 28.319220935289962 22.99093169482023 86-87 20.3940380160074 28.387053790852878 27.962544641341935 23.256363551797783 88-89 20.46974825038575 27.84361418295293 28.246237583002582 23.440399983658743 90-91 20.242725158695244 27.64615674690466 28.66626861919427 23.44484947520583 92-93 20.15426740269814 27.565476587083577 29.035926163857102 23.244329846361182 94-95 19.915424952530245 27.830319425422672 28.8973754457066 23.356880176340482 96-97 19.885593233656962 27.867225290181448 28.56347218249765 23.68370929366394 98-99 19.708057191466676 27.74060258393969 28.671494866134722 23.879845358458915 100-101 20.10086840389364 27.78974200651539 28.726116692205494 23.38327289738548 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1502.0 1 1048.5 2 657.5 3 742.5 4 675.5 5 706.0 6 1168.0 7 2333.0 8 3689.5 9 4150.0 10 3444.5 11 2691.5 12 2612.0 13 2736.5 14 2865.5 15 2935.0 16 3077.5 17 3238.0 18 3405.5 19 3563.5 20 3816.5 21 4180.0 22 4605.0 23 5082.0 24 5569.0 25 6399.5 26 7829.0 27 9502.5 28 10140.5 29 10939.5 30 12517.0 31 13522.5 32 14565.0 33 15731.0 34 17245.5 35 18671.0 36 19935.5 37 20728.0 38 19914.5 39 19064.5 40 19370.5 41 20035.0 42 20539.0 43 21943.5 44 23429.5 45 23968.0 46 24215.5 47 24780.5 48 27389.5 49 27856.0 50 25574.5 51 24048.0 52 22725.0 53 21872.5 54 20741.5 55 19777.5 56 19146.5 57 18132.0 58 17224.5 59 16525.5 60 15725.0 61 14713.5 62 12459.0 63 8465.5 64 5169.0 65 3661.5 66 2634.0 67 1842.5 68 1352.0 69 974.0 70 708.0 71 549.0 72 412.0 73 356.5 74 277.5 75 238.5 76 187.0 77 88.5 78 41.5 79 16.5 80 9.0 81 11.5 82 9.5 83 5.5 84 3.5 85 1.5 86 3.0 87 2.5 88 3.5 89 4.5 90 1.5 91 1.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038206458148218436 2 0.038709174702800254 3 0.01985730390598195 4 0.04122275747570936 5 0.06283956932272769 6 0.06057734482710949 7 0.061582777936273134 8 0.06409636070918225 9 0.06472475640240952 10-11 0.06918636582432319 12-13 0.069689082378905 14-15 0.062462531906791324 16-17 0.0526595590924458 18-19 0.04430189637252302 20-21 0.05467042531077309 22-23 0.027900768779291096 24-25 0.014767298790841005 26-27 0.01087124549283189 28-29 0.014201742666936459 30-31 0.010808405923509162 32-33 0.007289390041436411 34-35 0.008734700135859148 36-37 0.004712967699204576 38-39 0.007163710902790956 40-41 0.006598154778886408 42-43 0.0036446950207182057 44-45 9.425935398409153E-4 46-47 0.0025135827729091077 48-49 7.540748318727322E-4 50-51 0.004838646837850032 52-53 0.0044616094219136655 54-55 0.007415069180081867 56-57 0.020234341321918318 58-59 0.00735222961075914 60-61 0.019354587351400127 62-63 0.012693593003190994 64-65 0.008797539705181876 66-67 0.014327421805581912 68-69 0.023439159357377427 70-71 0.018411993811559214 72-73 0.017280881563750117 74-75 0.016275448454586474 76-77 0.023879036342636523 78-79 0.03368200915698204 80-81 0.029848795428295653 82-83 0.02934607887371383 84-85 0.019982983044627405 86-87 0.021365453569727414 88-89 0.0181606355342683 90-91 0.015961250607972835 92-93 0.03016299327490929 94-95 0.02142829313905014 96-97 0.03387052786495023 98-99 0.05259671952312307 100-101 0.06064018439643221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 795677.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.24949730164333 #Duplication Level Percentage of deduplicated Percentage of total 1 74.505692614384 37.43873599983556 2 13.743307437072428 13.811885799496512 3 4.971398019996606 7.4943075417364335 4 2.2990216112105366 4.620987209957741 5 1.264114663200443 3.176056317872921 6 0.7432127352798654 2.2407639801595534 7 0.5038758950200591 1.772365729902151 8 0.3083558923552957 1.23957828646826 9 0.24172442479645606 1.0931877748395724 >10 1.2524606047109814 11.41675081876164 >50 0.10185806437025152 3.591893914816393 >100 0.05895690670620458 5.7822768813483885 >500 0.003763206810560821 1.2948256010707646 >1k 0.0015052827242243284 1.184895903462576 >5k 2.508804540373881E-4 0.8340537607520376 >10k+ 5.017609080747761E-4 3.0074344795194397 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13724 1.7248204987702294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10132 1.2733810327557538 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6616 0.8314931812783328 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2221 0.2791333669315564 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2069 0.26003013785744716 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1626 0.20435427943751044 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1432 0.1799725265402921 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1046 0.13146037902314633 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1005 0.12630753433868266 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 935 0.11750999463350077 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 0.11650456152433714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.283956932272769E-5 0.0 12-13 0.0 0.0 0.0 1.2567913864545537E-4 0.0 14-15 0.0 0.0 0.0 1.8851870796818306E-4 0.0 16-17 0.0 0.0 0.0 2.5135827729091075E-4 0.0 18-19 0.0 0.0 0.0 3.1419784661363846E-4 0.0 20-21 0.0 0.0 0.0 3.770374159363661E-4 0.0 22-23 0.0 0.0 0.0 9.425935398409154E-4 0.0 24-25 0.0 0.0 0.0 0.0023879036342636523 0.0 26-27 0.0 0.0 0.0 0.0033304971741045675 0.0 28-29 0.0 0.0 0.0 0.007163710902790957 0.0 30-31 0.0 0.0 0.0 0.016463967162554653 0.0 32-33 0.0 0.0 0.0 0.02526150686773653 0.0 34-35 0.0 0.0 0.0 0.03242521777052749 0.0 36-37 0.0 0.0 0.0 0.03770374159363661 0.0 38-39 0.0 0.0 0.0 0.04235386972351846 0.0 40-41 0.0 0.0 0.0 0.05096289072073215 0.0 42-43 0.0 0.0 0.0 0.05869215774742766 0.0 44-45 0.0 0.0 0.0 0.06717549960599589 0.0 46-47 0.0 0.0 0.0 0.07898933863866871 0.0 48-49 0.0 0.0 0.0 0.09143157336456878 0.0 50-51 0.0 0.0 0.0 0.10670158870999161 0.0 52-53 0.0 0.0 0.0 0.15138052249845102 0.0 54-55 0.0 0.0 0.0 0.19932711389169222 0.0 56-57 0.0 0.0 0.0 0.26047001484270627 0.0 58-59 0.0 0.0 0.0 0.33053613463754766 0.0 60-61 0.0 0.0 0.0 0.43610661109973015 0.0 62-63 0.0 0.0 0.0 0.5015854423340125 0.0 64-65 0.0 0.0 0.0 0.5616600706065401 0.0 66-67 0.0 0.0 0.0 0.701289593641641 0.0 68-69 0.0 0.0 0.0 0.896972012512615 0.0 70-71 0.0 0.0 0.0 1.0106487934174295 0.0 72-73 0.0 0.0 0.0 1.128472985897544 0.0 74-75 0.0 1.2567913864545537E-4 0.0 1.3904511504039956 0.0 76-77 0.0 1.2567913864545537E-4 0.0 1.552828597533924 0.0 78-79 0.0 1.2567913864545537E-4 0.0 1.6747373620200157 0.0 80-81 0.0 1.2567913864545537E-4 0.0 1.8408851833093078 0.0 82-83 0.0 1.2567913864545537E-4 0.0 2.1445888218460505 0.0 84-85 0.0 1.2567913864545537E-4 0.0 2.4548277755923573 0.0 86-87 0.0 1.2567913864545537E-4 0.0 2.9320314650291515 0.0 88-89 0.0 1.2567913864545537E-4 0.0 3.5306412023974554 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCACT 35 2.7782924E-4 54.32177 4 GGTATCA 5500 0.0 47.704395 1 GTATCAA 10250 0.0 43.682655 1 TATCAAC 13530 0.0 33.092922 2 ATCAACG 13485 0.0 33.022957 3 TCAACGC 13560 0.0 32.879498 4 CAACGCA 13790 0.0 32.30274 5 CGTCACT 180 5.456968E-12 31.687702 1 AACGCAG 14125 0.0 31.568289 6 ACGCAGA 15715 0.0 28.011301 7 CGCAGAG 15730 0.0 27.893925 8 GCAGAGT 16020 0.0 27.092241 9 GTACATG 8955 0.0 27.016819 1 TACATGG 8870 0.0 26.847023 2 TCTCGCA 90 8.910497E-4 26.406418 2 CATGGGT 1070 0.0 26.208986 4 ACATGGG 9125 0.0 25.208084 3 AGTACTT 9600 0.0 24.602837 12-13 GAGTACT 9575 0.0 24.56781 12-13 CATGGGA 4470 0.0 23.393604 4 >>END_MODULE