FastQCFastQC Report
Thu 26 May 2016
SRR936994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795677
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142521.7911790839750301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110971.3946614015486183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76200.95767503647837No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.3517759090686296No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24900.3129410552271839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23010.28918769802319283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.21868170124309236No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.1309576624685645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10150.1275643257251372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9520.11964653999047353No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.1129855456422644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8980.11285986650361893No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8760.1100949254534189No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.10217713971875522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8090.1016744231641734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63000.049.637841
GTATCAA112950.041.1509781
TATCAAC149050.031.1665482
ATCAACG148900.031.0983353
TCAACGC151250.030.6151564
CAACGCA153400.030.1551065
AACGCAG156450.029.567236
ACGCAGA176050.026.032667
CGCAGAG176250.025.9222818
GCAGAGT181600.024.844779
GAGTACT109350.024.21326412-13
AGTACTT109300.023.68119212-13
GTACATG91750.023.568551
TACATGG91400.023.3336122
GTACTTT115650.022.8121214-15
GACCGCG1050.002197582522.6155917
ACATGGG92550.021.80923
GTTAGTC1100.002879148321.587613
TTAGGCA2657.930794E-1021.5061474
AGAGTAC170400.021.26582110-11
Produced by FastQC (version 0.11.5)