##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936994_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 795677 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1194027224615 31.0 31.0 34.0 31.0 34.0 2 32.1486947593056 33.0 31.0 34.0 30.0 34.0 3 32.23512430295208 33.0 31.0 34.0 30.0 34.0 4 35.991711460806336 37.0 35.0 37.0 35.0 37.0 5 35.937595280496986 37.0 35.0 37.0 35.0 37.0 6 36.0073019579553 37.0 35.0 37.0 35.0 37.0 7 35.95348992116148 37.0 35.0 37.0 35.0 37.0 8 35.946479538807836 37.0 35.0 37.0 35.0 37.0 9 37.58602422842435 39.0 37.0 39.0 35.0 39.0 10-11 37.50090049102839 39.0 37.0 39.0 35.0 39.0 12-13 37.40083853121304 39.0 37.0 39.0 34.0 39.0 14-15 38.43412276589621 40.0 38.0 41.0 34.0 41.0 16-17 38.409528615254686 40.0 38.0 41.0 34.0 41.0 18-19 38.592123437022806 40.0 38.0 41.0 34.0 41.0 20-21 38.71432440550626 40.0 38.0 41.0 34.5 41.0 22-23 38.624319290365314 40.0 38.0 41.0 34.0 41.0 24-25 38.50159109789526 40.0 38.0 41.0 34.0 41.0 26-27 38.28127745303685 40.0 37.5 41.0 34.0 41.0 28-29 38.07046326587296 40.0 37.0 41.0 33.5 41.0 30-31 37.73730609279896 39.5 36.5 41.0 33.0 41.0 32-33 37.37215540979568 39.0 35.0 41.0 33.0 41.0 34-35 37.105701811162064 39.0 35.0 41.0 33.0 41.0 36-37 36.809876997827004 39.0 35.0 41.0 32.5 41.0 38-39 36.53466796199966 39.0 35.0 41.0 31.5 41.0 40-41 36.1321233364795 39.0 35.0 41.0 30.5 41.0 42-43 35.64269043845681 39.0 35.0 41.0 29.5 41.0 44-45 34.96000638450025 38.5 35.0 41.0 25.5 41.0 46-47 33.99010968018429 38.0 34.0 40.0 14.5 41.0 48-49 33.13693684748962 38.0 33.0 40.0 2.0 41.0 50-51 32.68198464955 38.0 33.0 40.0 2.0 41.0 52-53 32.38126463376471 38.0 33.0 40.0 2.0 41.0 54-55 32.056576349448335 37.0 32.5 40.0 2.0 41.0 56-57 31.764653873368214 37.0 32.0 40.0 2.0 41.0 58-59 31.406421198551673 36.0 31.0 40.0 2.0 41.0 60-61 31.048177212612657 36.0 31.0 39.5 2.0 41.0 62-63 30.69087267823501 35.0 31.0 39.0 2.0 40.5 64-65 30.278932280309725 35.0 30.0 39.0 2.0 40.0 66-67 29.85670630167769 35.0 29.0 38.0 2.0 40.0 68-69 29.411748108843163 35.0 29.0 37.5 2.0 40.0 70-71 29.037818738005498 35.0 29.0 37.0 2.0 39.0 72-73 28.68910500114996 34.0 28.0 36.5 2.0 39.0 74-75 28.25029126140381 34.0 27.5 36.0 2.0 39.0 76-77 26.876322930033165 32.5 25.5 35.0 2.0 36.5 78-79 27.517677399246175 34.0 26.0 35.0 2.0 37.0 80-81 27.25194645565977 34.0 26.0 35.0 2.0 37.0 82-83 27.05821960418612 34.0 26.0 35.0 2.0 36.0 84-85 26.7665943592689 34.0 25.0 35.0 2.0 36.0 86-87 26.423199991956537 33.0 24.5 35.0 2.0 35.5 88-89 26.070638588271372 33.0 23.5 35.0 2.0 35.0 90-91 25.657759367180404 33.0 20.5 35.0 2.0 35.0 92-93 25.275784017886654 33.0 18.5 35.0 2.0 35.0 94-95 24.839823822983448 32.5 12.5 35.0 2.0 35.0 96-97 24.27736568984651 32.0 2.0 34.5 2.0 35.0 98-99 23.62525559994822 32.0 2.0 34.0 2.0 35.0 100-101 20.986387064097617 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39016488557301443 1101 2 0.19781717517160047 1101 3 0.20766331163548557 1101 4 0.26736369074883015 1101 5 0.28553928355719904 1101 6 0.3548899365163436 1101 7 0.26268452609232185 1101 8 0.21272258732866334 1101 9 0.4343168135667739 1101 10-11 0.3800437369227794 1101 12-13 0.33532942219062534 1101 14-15 0.5115040081538424 1101 16-17 0.35379098243747364 1101 18-19 0.5047358083447193 1101 20-21 0.3559032175186303 1101 22-23 0.4382444959707499 1101 24-25 0.4258455024672969 1101 26-27 0.46206467616470803 1101 28-29 0.4500613700442031 1101 30-31 0.23510462460487958 1101 32-33 0.49951633874903223 1101 34-35 0.487055441562795 1101 36-37 0.35341970885900054 1101 38-39 0.3812530205576863 1101 40-41 0.23498655614598363 1101 42-43 0.2788502508853128 1101 44-45 0.14713628054196448 1101 46-47 -0.042144798398787486 1101 48-49 0.20809205785203488 1101 50-51 0.014840834663765179 1101 52-53 0.026408279117710265 1101 54-55 0.26227677549027817 1101 56-57 0.07724639181083504 1101 58-59 0.04891010962653297 1101 60-61 -0.01090082271558046 1101 62-63 -0.24469456965083936 1101 64-65 0.06307371684706453 1101 66-67 -0.1891824221374172 1101 68-69 -0.03163982876246507 1101 70-71 0.1958797803093617 1101 72-73 0.3036648586683093 1101 74-75 0.2912231994794716 1101 76-77 0.12026515353963774 1101 78-79 0.16669714669961877 1101 80-81 0.4225039102344219 1101 82-83 0.4447193184360536 1101 84-85 0.2303553575293762 1101 86-87 0.05851988028034327 1101 88-89 0.11522542555050919 1101 90-91 0.01295894091801486 1101 92-93 0.12876705441907177 1101 94-95 0.09918979537367179 1101 96-97 -0.1490640679752424 1101 98-99 0.4683663497034338 1101 100-101 0.8322521327503409 1102 1 0.13580270223430801 1102 2 0.11843813905291967 1102 3 0.07272627752936955 1102 4 0.05789096294321183 1102 5 -0.0376774969267899 1102 6 0.203339480758558 1102 7 0.19702462984682967 1102 8 0.1462493612669391 1102 9 0.14019916650794784 1102 10-11 0.1352925230341313 1102 12-13 0.1812119705263484 1102 14-15 0.23998374085409324 1102 16-17 0.1041671169470888 1102 18-19 0.10069040842091681 1102 20-21 -0.04407553332400482 1102 22-23 0.04998941815487257 1102 24-25 -0.05699274992385028 1102 26-27 0.04860858620506292 1102 28-29 -0.09747201799960692 1102 30-31 -0.029731927853674733 1102 32-33 -0.19935012861518686 1102 34-35 -0.10037140760238117 1102 36-37 -0.20837748136736423 1102 38-39 -0.3634981933309618 1102 40-41 -0.11201706842292936 1102 42-43 -0.36055887599414405 1102 44-45 -0.37573465158774155 1102 46-47 -0.24914456392533424 1102 48-49 0.1649477198976257 1102 50-51 0.014590485396006159 1102 52-53 -0.018085502450723823 1102 54-55 -0.3013314779755305 1102 56-57 -0.12679558870832608 1102 58-59 -0.17958061401718695 1102 60-61 -0.2328946775569669 1102 62-63 -0.24080499688102464 1102 64-65 -0.16267537701070722 1102 66-67 -0.24832957377335774 1102 68-69 -0.2635817086534331 1102 70-71 -0.3528357447646471 1102 72-73 -0.17877649854223776 1102 74-75 0.00435713267407678 1102 76-77 -0.14336972243367185 1102 78-79 -0.21488520721852922 1102 80-81 -0.25170498607946357 1102 82-83 0.038133789356457726 1102 84-85 -0.32319374023628455 1102 86-87 -0.24071344035234432 1102 88-89 -0.18711100478644838 1102 90-91 -0.10825895331491253 1102 92-93 -0.00899098850911173 1102 94-95 0.05274451073265851 1102 96-97 0.22575885571341558 1102 98-99 0.35917010518669557 1102 100-101 0.7959319494702513 1103 1 0.19064101182715376 1103 2 0.08309651702398924 1103 3 0.13321831059990785 1103 4 0.1444378624047502 1103 5 0.09158707439881653 1103 6 0.15973958465976779 1103 7 0.11347542068948258 1103 8 0.16112899733189323 1103 9 0.14215784471607407 1103 10-11 0.2229305022024093 1103 12-13 0.29159179497074916 1103 14-15 0.18160775888235747 1103 16-17 0.27808522220088605 1103 18-19 0.39128734895152206 1103 20-21 0.3830023153593274 1103 22-23 0.28101867196166097 1103 24-25 0.315353264505319 1103 26-27 0.3994126505208442 1103 28-29 0.36149694970443846 1103 30-31 0.489476340248892 1103 32-33 0.4247696947527544 1103 34-35 0.5599482758265424 1103 36-37 0.6441342835725266 1103 38-39 0.5882643830446241 1103 40-41 0.6755622908061412 1103 42-43 0.7520335293890668 1103 44-45 0.6807095635252054 1103 46-47 0.734666504361634 1103 48-49 0.71167986304728 1103 50-51 0.6191966553493629 1103 52-53 0.5800947053048873 1103 54-55 0.4641150515134598 1103 56-57 0.42798129280451036 1103 58-59 0.5930758169762669 1103 60-61 0.37074736405500275 1103 62-63 0.530660899419015 1103 64-65 0.4746134566514151 1103 66-67 0.3678040141647436 1103 68-69 0.5689144477038468 1103 70-71 0.5150728221709002 1103 72-73 0.4805480499906274 1103 74-75 0.4461484690916855 1103 76-77 0.6103025066918981 1103 78-79 0.5456564363394776 1103 80-81 0.9655912891557783 1103 82-83 0.9131744488951234 1103 84-85 0.8665131366665193 1103 86-87 0.8186763974541265 1103 88-89 0.8544120371788715 1103 90-91 0.7839932692115248 1103 92-93 0.778935878143983 1103 94-95 0.8930081015491034 1103 96-97 1.08899303827533 1103 98-99 1.2966550406813617 1103 100-101 1.441529369724904 1104 1 0.14960779692175663 1104 2 0.18893001101537976 1104 3 0.020271107427142 1104 4 0.0796984038682993 1104 5 -0.018593367548547235 1104 6 0.08305290458962844 1104 7 -0.018221725105412645 1104 8 0.0473303064655326 1104 9 0.18004330929326784 1104 10-11 0.11130643287157227 1104 12-13 0.2020243553093195 1104 14-15 0.21374568152780427 1104 16-17 0.07113125302886658 1104 18-19 -0.03414493597324508 1104 20-21 -0.03458584001078435 1104 22-23 -0.04418478998992725 1104 24-25 -0.07558458836862769 1104 26-27 -0.016457611214072188 1104 28-29 -0.13848927960510338 1104 30-31 0.08252087925207974 1104 32-33 -0.19293987220493136 1104 34-35 -0.08162245502990118 1104 36-37 0.050777035140093574 1104 38-39 0.18997298146374675 1104 40-41 0.1737944784180243 1104 42-43 0.258879283885193 1104 44-45 0.4309625999624487 1104 46-47 0.489448950403478 1104 48-49 0.570448809222242 1104 50-51 0.5540785028251989 1104 52-53 0.5350295469878503 1104 54-55 0.5682323732059729 1104 56-57 0.48090090869405344 1104 58-59 0.4493786620009139 1104 60-61 0.6045340973718076 1104 62-63 0.46475265352956896 1104 64-65 0.4230565653388858 1104 66-67 0.5312288307850501 1104 68-69 0.33191225816406345 1104 70-71 0.36153077107245934 1104 72-73 0.540607195106162 1104 74-75 0.8752512168034521 1104 76-77 0.6612850454563919 1104 78-79 0.646896257504114 1104 80-81 0.6310983489297577 1104 82-83 0.7333533301642312 1104 84-85 0.6772671278716409 1104 86-87 0.5755468244373318 1104 88-89 0.7486502655336444 1104 90-91 0.5193596154801234 1104 92-93 0.482797179749646 1104 94-95 0.7499122667239426 1104 96-97 0.7399829214080711 1104 98-99 0.6513604856123685 1104 100-101 0.8468788239465397 1105 1 0.06961069499246975 1105 2 0.18145597864899088 1105 3 0.16409821680861114 1105 4 0.1317774245964003 1105 5 0.14654031171323112 1105 6 0.13962278489470492 1105 7 0.12004959871489262 1105 8 0.12044679050774221 1105 9 0.07156116232888365 1105 10-11 0.1318562842879416 1105 12-13 0.14717204191288147 1105 14-15 0.17532956917465725 1105 16-17 0.2417919671502915 1105 18-19 0.2377694237371344 1105 20-21 0.23837907033303196 1105 22-23 0.027796041342355693 1105 24-25 0.28313273933417094 1105 26-27 0.25764927699399465 1105 28-29 0.20627073253918837 1105 30-31 0.10719875639227183 1105 32-33 0.07220829741040546 1105 34-35 0.33411843904791994 1105 36-37 0.164320455599551 1105 38-39 0.2875327369217757 1105 40-41 0.3951122035705197 1105 42-43 0.301158340494176 1105 44-45 0.27985408791475663 1105 46-47 0.03736774462205972 1105 48-49 0.23425995621422402 1105 50-51 0.24231854588224877 1105 52-53 0.2557377572681858 1105 54-55 0.09901114920810272 1105 56-57 0.08103500196592961 1105 58-59 0.43354967577365144 1105 60-61 0.3171093092069803 1105 62-63 0.301679249538509 1105 64-65 0.28710312307488906 1105 66-67 0.06932581035134788 1105 68-69 -0.04170598312102314 1105 70-71 -0.002272555728715986 1105 72-73 0.06301721691069417 1105 74-75 -0.060155157112067315 1105 76-77 0.040024936709734504 1105 78-79 0.21131392929530435 1105 80-81 0.45077337400036654 1105 82-83 0.44947695358064266 1105 84-85 0.17550528488267148 1105 86-87 0.23657490352246313 1105 88-89 0.19359094708627467 1105 90-91 0.24866371050004332 1105 92-93 0.41012460653885796 1105 94-95 0.24303509892105168 1105 96-97 0.4802410337060117 1105 98-99 0.507740381281824 1105 100-101 0.5679418178835896 1106 1 0.1754498041994097 1106 2 0.14485156470920657 1106 3 0.0524281692871682 1106 4 0.1572244257012514 1106 5 0.1516169227112485 1106 6 0.10190286650889391 1106 7 0.15547929884314016 1106 8 0.16362462820539747 1106 9 0.22529591147656447 1106 10-11 0.2832303560331155 1106 12-13 0.15977061070409349 1106 14-15 0.4542625216733498 1106 16-17 0.40857942092257815 1106 18-19 0.42836141081528467 1106 20-21 0.3539539755092278 1106 22-23 0.4045807596419593 1106 24-25 0.3035143792204451 1106 26-27 0.3216569694747662 1106 28-29 0.2814565611328135 1106 30-31 0.18090139038010733 1106 32-33 0.38645845224289843 1106 34-35 0.40895256401046964 1106 36-37 0.4387244837311073 1106 38-39 0.4478059763016873 1106 40-41 0.36817016598678265 1106 42-43 0.446760091196694 1106 44-45 0.29199437621699786 1106 46-47 0.3307104804026366 1106 48-49 0.3384631106523912 1106 50-51 0.16890248522343398 1106 52-53 -0.08338565991745384 1106 54-55 0.0818303676207961 1106 56-57 0.10736067005480265 1106 58-59 0.22432154090418877 1106 60-61 0.17423770234769265 1106 62-63 0.13521152633992628 1106 64-65 0.3506661532207467 1106 66-67 0.08986257877522874 1106 68-69 0.03268762416220028 1106 70-71 0.3618798444413329 1106 72-73 0.25860351396881853 1106 74-75 0.3648319056415694 1106 76-77 0.1442227623049952 1106 78-79 0.08289947321030056 1106 80-81 0.37656568412481306 1106 82-83 0.5512110774774222 1106 84-85 0.4342697512108984 1106 86-87 0.39623947062503007 1106 88-89 0.34128693304655044 1106 90-91 0.6733961729820273 1106 92-93 0.6575031118813186 1106 94-95 0.6228495604533144 1106 96-97 0.6513288636946051 1106 98-99 1.0041149918322212 1106 100-101 1.0177766829632091 1107 1 0.003212252575394814 1107 2 -0.03492805259678278 1107 3 -0.024705773615316673 1107 4 0.1355937380423171 1107 5 0.23957883365644506 1107 6 0.08377449629482925 1107 7 0.04119049291336552 1107 8 0.09954412179410355 1107 9 0.16765014690010815 1107 10-11 0.10887193658461314 1107 12-13 0.10544140729194851 1107 14-15 0.1262421786554455 1107 16-17 0.25816011669707706 1107 18-19 0.24216688972382627 1107 20-21 0.1973848759763257 1107 22-23 0.0767010864287414 1107 24-25 0.09298939229552161 1107 26-27 0.023363041721331967 1107 28-29 0.318719631702173 1107 30-31 0.3451189596780253 1107 32-33 0.30811799524637706 1107 34-35 0.3027162026694086 1107 36-37 0.33159180324994253 1107 38-39 0.4182779415309952 1107 40-41 0.5511851888005381 1107 42-43 0.41334019182971105 1107 44-45 0.4548539408619874 1107 46-47 0.5802136274386456 1107 48-49 0.9726801389125868 1107 50-51 0.9285320547377651 1107 52-53 0.8253178333826909 1107 54-55 0.9417629182529055 1107 56-57 0.9383585239385539 1107 58-59 0.7104547443884748 1107 60-61 0.7563888682839561 1107 62-63 0.8308886350344018 1107 64-65 0.8215240586834263 1107 66-67 0.49590236308153024 1107 68-69 0.5501919975503604 1107 70-71 0.4845691625534734 1107 72-73 0.6618713817391999 1107 74-75 0.759975190461855 1107 76-77 0.8842884082138518 1107 78-79 1.0163301933314877 1107 80-81 1.140316157532812 1107 82-83 0.9736118421440558 1107 84-85 1.0221739514669892 1107 86-87 1.1616509298037343 1107 88-89 0.7678302431152595 1107 90-91 1.0483984273058233 1107 92-93 1.207891757303237 1107 94-95 1.289074379656551 1107 96-97 1.2652281224729442 1107 98-99 1.4734706159192221 1107 100-101 1.4338761617635498 1108 1 0.1371967498190827 1108 2 -0.03769279104795231 1108 3 0.051616414102845454 1108 4 0.04613633451327814 1108 5 -0.07956610748604476 1108 6 0.024918185661960024 1108 7 0.13025430938383664 1108 8 0.043696886532551105 1108 9 -0.06017657850370739 1108 10-11 0.08747741069453241 1108 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-0.4859243433817362 2316 84-85 -0.6312069184072833 2316 86-87 -0.5885401048934966 2316 88-89 -0.40896126250471454 2316 90-91 -0.44838063884549584 2316 92-93 -0.3357352363437478 2316 94-95 -0.4160768931676522 2316 96-97 -0.5867577498447929 2316 98-99 -0.48080545143161757 2316 100-101 -0.7892479219363047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 16.0 5 45.0 6 66.0 7 167.0 8 413.0 9 820.0 10 1645.0 11 2929.0 12 4832.0 13 6351.0 14 8154.0 15 12004.0 16 17166.0 17 24683.0 18 20558.0 19 9205.0 20 6308.0 21 5787.0 22 5795.0 23 5985.0 24 6783.0 25 7666.0 26 8862.0 27 10535.0 28 12827.0 29 15536.0 30 18912.0 31 23360.0 32 29266.0 33 38019.0 34 52621.0 35 75248.0 36 106727.0 37 149474.0 38 100329.0 39 6577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.70667715957348 19.388075800356525 15.968787792693014 17.93645924737698 2 16.605540333324953 22.30034438551067 39.76345491566325 21.33066036550112 3 19.19724782173688 29.038145371725065 31.824512184698612 19.940094621839442 4 12.328872142841881 18.56029519516085 37.41329710422697 31.6975355577703 5 11.749608803031542 40.085214954093274 31.743324514381595 16.421851728493593 6 27.044893845115542 38.3318859285866 17.79955936893991 16.823660857357947 7 25.246802408515013 38.07386665694748 19.55919298911493 17.120137945422577 8 28.165610122713264 31.504909033325124 19.35989020656649 20.96959063739512 9 30.038947965066225 14.85929592032948 20.32822363848647 34.77353247611782 10-11 25.240581291152065 28.00093505279152 25.034530343342837 21.723953312713576 12-13 28.16814140940622 26.826137037020747 26.829907418287984 18.17581413528505 14-15 24.574481856331147 25.654128496864935 29.388872620422607 20.382517026381308 16-17 19.797995793516588 29.0130473879774 29.047797691649812 22.141159126856202 18-19 19.553191288801187 24.80410485865197 36.60984675906578 19.032857093481052 20-21 22.074114943453278 23.18230119415159 36.791972043668885 17.951611818726242 22-23 21.249828762173596 23.67794972080379 36.714332574650264 18.357888942372345 24-25 20.11511516543956 24.072945233887776 37.36894165437045 18.44299794630221 26-27 19.854217438354425 24.253836862790443 37.336498931045185 18.555446767809944 28-29 19.67558551968407 24.50704986563904 37.02913961222597 18.788225002450925 30-31 20.000012571705867 24.345171270634882 36.99230737317977 18.662508784479474 32-33 19.436954274528777 24.563246813230318 36.82381884506503 19.175980067175878 34-35 19.8710097449668 24.570425248054555 36.874396320453364 18.684168686525275 36-37 20.25429981761206 24.394606546105432 36.18735049782731 19.163743138455196 38-39 20.12669444392074 24.835567454107828 35.917372095800104 19.120366006171327 40-41 20.245874543902847 25.0085154893924 35.56043232291238 19.185177643792365 42-43 20.113270296460996 25.39808626993574 34.946626463168926 19.542016970434343 44-45 20.63044793827487 25.468088301924258 34.16574866163979 19.735715098161076 46-47 20.989790903937973 25.901355221997253 32.82457522845728 20.2842786456075 48-49 21.28116039295605 26.58297529791249 31.39733416785907 20.73853014127239 50-51 21.521191585202466 27.218386066165102 30.477750155219784 20.782672193412647 52-53 21.672444921200192 27.486456135852766 29.849664836100548 20.991434106846494 54-55 21.521149383249032 27.323302685878353 29.62805162748229 21.527496303390322 56-57 21.999607878130533 27.76939212312627 28.772759092640047 21.45824090610315 58-59 21.970065441785465 27.29470420488922 29.13045500076037 21.604775352564946 60-61 22.07453526996507 27.314224239232754 29.579779232025054 21.031461258777117 62-63 22.379509763075824 27.303370151060136 29.598021306397765 20.71909877946628 64-65 22.176506335668844 27.510308070051515 29.616541679418862 20.696643914860775 66-67 22.390074326922836 27.80403783459055 29.003963934978394 20.80192390350822 68-69 22.564807343784953 27.695352069838346 28.98075323062692 20.759087355749774 70-71 22.243134662359054 27.811066611952185 29.133389178974806 20.81240954671396 72-73 22.160394979298278 28.049701729291364 28.73661678310076 21.053286508309597 74-75 21.593418540550935 28.34323535215534 28.917497979585967 21.145848127707765 76-77 21.20498963111921 28.40633444353673 29.13529818387482 21.25337774146924 78-79 21.076933229008567 28.26233278971333 28.983179202921093 21.67755477835701 80-81 21.032668427432505 28.40337750329437 28.805613839292725 21.758340229980398 82-83 21.09166046614515 28.381274257445654 28.527698735093608 21.99936654131558 84-85 20.950056461956205 28.036550397558784 28.460093619390488 22.553299521094527 86-87 20.660582120634377 28.238719983108723 28.12963049076447 22.97106740549243 88-89 20.886342027754196 27.83349996575185 28.358973359969387 22.921184646524566 90-91 20.59636570209113 27.877215351348774 28.701741112358835 22.82467783420126 92-93 20.396078801018003 27.7657963364439 28.9777233166808 22.860401545857293 94-95 20.199190494055014 28.115658428960227 28.761465958584616 22.92368511840014 96-97 20.23476922110158 28.261790303830082 28.50793351556854 22.995506959499796 98-99 19.94681252167968 27.98431773737049 28.536364047677697 23.532505693272135 100-101 19.997561809388795 28.60933902412049 28.42025357182357 22.97284559466715 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2502.0 1 1687.5 2 918.5 3 951.5 4 781.0 5 720.5 6 1164.0 7 2398.0 8 3823.5 9 4239.5 10 3459.0 11 2647.5 12 2593.5 13 2757.0 14 2866.5 15 3012.5 16 3160.5 17 3353.0 18 3560.0 19 3754.5 20 3954.5 21 4339.0 22 4925.0 23 5435.5 24 5914.5 25 6666.5 26 8071.0 27 9667.0 28 10456.0 29 11354.0 30 12635.5 31 13440.5 32 14350.0 33 15430.5 34 16994.0 35 18533.0 36 19893.5 37 20511.5 38 19457.5 39 18931.0 40 19382.5 41 19867.0 42 20514.5 43 22048.5 44 23938.0 45 24599.5 46 24413.5 47 24858.0 48 27397.0 49 27468.5 50 25048.0 51 23453.0 52 21628.5 53 20832.0 54 20286.5 55 19506.5 56 18939.0 57 18103.5 58 17378.0 59 16860.5 60 15787.5 61 14319.5 62 12117.0 63 8387.5 64 5209.0 65 3645.0 66 2533.5 67 1748.5 68 1351.5 69 1030.0 70 705.5 71 523.5 72 444.5 73 410.0 74 301.5 75 225.5 76 174.5 77 87.0 78 39.5 79 21.5 80 11.0 81 10.5 82 7.0 83 1.0 84 0.5 85 2.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027523731363354724 2 0.007163710902790956 3 0.011185443339445529 4 0.0 5 0.005278523823109125 6 0.0 7 0.0 8 1.2567913864545537E-4 9 0.0 10-11 0.0 12-13 1.8851870796818306E-4 14-15 0.0 16-17 6.283956932272769E-5 18-19 0.00433593028326821 20-21 9.425935398409153E-4 22-23 0.0 24-25 0.00490148640717276 26-27 0.005027165545818215 28-29 0.007415069180081867 30-31 0.03016299327490929 32-33 5.655561239045492E-4 34-35 0.0044616094219136655 36-37 0.015081496637454644 38-39 0.007792106596018233 40-41 0.008546181427890966 42-43 0.007289390041436411 44-45 0.002576422342231835 46-47 0.0017595079410363751 48-49 0.0019480266490045583 50-51 0.0038960532980091165 52-53 0.0027021014808772904 54-55 0.0019480266490045583 56-57 7.540748318727322E-4 58-59 0.001005433109163643 60-61 0.0 62-63 6.283956932272769E-5 64-65 0.007854946165340961 66-67 3.770374159363661E-4 68-69 0.001382470525100009 70-71 0.003958892867331845 72-73 0.01143680161673644 74-75 0.006032598654981858 76-77 0.0033933367434272953 78-79 0.010619887215540979 80-81 6.283956932272769E-5 82-83 0.005655561239045492 84-85 0.0010682726784863707 86-87 0.0 88-89 0.0016966683717136476 90-91 9.425935398409153E-4 92-93 2.5135827729091075E-4 94-95 8.1691440119546E-4 96-97 2.5135827729091075E-4 98-99 1.2567913864545537E-4 100-101 6.283956932272769E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 795677.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.55628133242339 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52817704545033 35.702548504791224 2 14.149380578754378 13.740826081230505 3 5.161317608176043 7.518431694857597 4 2.3977930993641783 4.657116652386819 5 1.3080070701207447 3.175597964079087 6 0.7989627124379884 2.327679494355303 7 0.5341142183426159 1.8154220174644027 8 0.35952177179432565 1.3965632237101278 9 0.25539210285289937 1.1160801716584134 >10 1.3294078665225977 11.728173845636624 >50 0.10670395985520939 3.6773531616078103 >100 0.06448825314886358 6.09008309945116 >500 0.004402812463750116 1.4761170277390276 >1k 0.0015539338107353352 1.4319768780767004 >5k 2.589889684558892E-4 0.9582562405325772 >10k+ 5.179779369117784E-4 3.1877739424226115 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14252 1.7911790839750301 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11097 1.3946614015486183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7620 0.95767503647837 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 0.3517759090686296 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2490 0.3129410552271839 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2301 0.28918769802319283 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1740 0.21868170124309236 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1042 0.1309576624685645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1015 0.1275643257251372 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 952 0.11964653999047353 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 899 0.1129855456422644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 898 0.11285986650361893 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 876 0.1100949254534189 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 813 0.10217713971875522 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 809 0.1016744231641734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.283956932272769E-5 0.0 12-13 0.0 0.0 0.0 1.2567913864545537E-4 0.0 14-15 0.0 0.0 0.0 1.8851870796818306E-4 0.0 16-17 0.0 0.0 0.0 3.1419784661363846E-4 0.0 18-19 0.0 0.0 0.0 4.398769852590938E-4 0.0 20-21 0.0 0.0 0.0 5.027165545818215E-4 0.0 22-23 0.0 0.0 0.0 0.0010682726784863707 0.0 24-25 0.0 0.0 0.0 0.0025135827729091077 0.0 26-27 0.0 0.0 0.0 0.0033933367434272953 0.0 28-29 0.0 0.0 0.0 0.007477908749404596 0.0 30-31 0.0 0.0 0.0 0.01696668371713648 0.0 32-33 0.0 0.0 0.0 0.025952742130286536 0.0 34-35 0.0 0.0 0.0 0.03330497174104567 0.0 36-37 0.0 0.0 0.0 0.038772014272122984 0.0 38-39 0.0 0.0 0.0 0.04342214240200483 0.0 40-41 0.0 0.0 0.0 0.05259671952312307 0.0 42-43 0.0 0.0 0.0 0.0608287031044004 0.0 44-45 0.0 0.0 0.0 0.0694377241016141 0.0 46-47 0.0 0.0 0.0 0.08137724227293236 0.0 48-49 0.0 0.0 0.0 0.09413367484544607 0.0 50-51 0.0 0.0 0.0 0.10890097363628709 0.0 52-53 0.0 0.0 0.0 0.15383126570203737 0.0 54-55 0.0 0.0 0.0 0.20278329020444225 0.0 56-57 0.0 0.0 0.0 0.2649316242646199 0.0 58-59 0.0 0.0 0.0 0.33575181889133404 0.0 60-61 0.0 0.0 0.0 0.44239056803200294 0.0 62-63 0.0 0.0 0.0 0.5084349553901897 0.0 64-65 0.0 0.0 0.0 0.568383904524072 0.0 66-67 0.0 0.0 0.0 0.7090817002376593 0.0 68-69 0.0 0.0 0.0 0.9057695522177969 0.0 70-71 0.0 0.0 0.0 1.018629418721416 0.0 72-73 0.0 0.0 0.0 1.1363907716322075 0.0 74-75 0.0 0.0 0.0 1.3983060965693366 0.0 76-77 0.0 0.0 0.0 1.559803789728747 0.0 78-79 0.0 0.0 0.0 1.6809584793829657 0.0 80-81 0.0 0.0 0.0 1.8457866697164804 0.0 82-83 0.0 0.0 0.0 2.150935618347646 0.0 84-85 0.0 0.0 0.0 2.4616772886485343 0.0 86-87 0.0 0.0 0.0 2.9400749299024604 0.0 88-89 0.0 0.0 0.0 3.5403813356424783 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6300 0.0 49.63784 1 GTATCAA 11295 0.0 41.150978 1 TATCAAC 14905 0.0 31.166548 2 ATCAACG 14890 0.0 31.098335 3 TCAACGC 15125 0.0 30.615156 4 CAACGCA 15340 0.0 30.155106 5 AACGCAG 15645 0.0 29.56723 6 ACGCAGA 17605 0.0 26.03266 7 CGCAGAG 17625 0.0 25.922281 8 GCAGAGT 18160 0.0 24.84477 9 GAGTACT 10935 0.0 24.213264 12-13 AGTACTT 10930 0.0 23.681192 12-13 GTACATG 9175 0.0 23.56855 1 TACATGG 9140 0.0 23.333612 2 GTACTTT 11565 0.0 22.81212 14-15 GACCGCG 105 0.0021975825 22.615591 7 ACATGGG 9255 0.0 21.8092 3 GTTAGTC 110 0.0028791483 21.58761 3 TTAGGCA 265 7.930794E-10 21.506147 4 AGAGTAC 17040 0.0 21.265821 10-11 >>END_MODULE