FastQCFastQC Report
Thu 26 May 2016
SRR936993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821866
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140331.7074559599740105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101741.2379146965563728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67120.816678144612382No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.25697619806635147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20990.2553944316956779No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.20769809190305963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.17873959988611282No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10420.1267846583262965No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.1152255963867589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9400.11437387603331929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9020.10975025125750427No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.1019630937403421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52900.054.0684361
GTATCAA102300.045.3291051
TATCAAC134550.034.364612
CGTCACT2100.033.9371151
ATCAACG136350.033.910953
TCAACGC136700.033.7893644
CAACGCA139400.033.100825
AACGCAG142450.032.4254576
ATAACGC1051.8943665E-631.6804263
CGCAGAG161150.028.3366768
ACGCAGA162050.028.3259147
GCAGAGT163850.027.3477179
GTACATG94450.025.1016061
GAGTACT98850.025.01767712-13
TACATGG94550.024.8283392
AGTACTT99350.024.46136712-13
CATGGGT11850.023.6600654
GTCACTA3450.023.4159662
ACATGGG96700.023.3917523
GTACTTT105450.023.36031514-15
Produced by FastQC (version 0.11.5)