FastQCFastQC Report
Thu 26 May 2016
SRR936993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821866
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144131.7536922077321608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113351.3791786008911429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76250.9277668135681486No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.33849800332414287No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.3118513236951036No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23100.2810677166350719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17100.20806311491167662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10160.12362112558494938No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.12094429018842487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9340.11364383001608534No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.11364383001608534No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9190.11181871497300047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8740.10634336984374582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8690.10573499816271753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64000.049.591781
GTATCAA115900.042.798671
TATCAAC153800.032.2074432
ATCAACG154700.031.9586683
TCAACGC156700.031.581084
CAACGCA159900.030.9584775
AACGCAG164050.030.2042776
CATGGGT11500.027.6697484
ACGCAGA183600.026.8587047
CGCAGAG183650.026.8255238
GCAGAGT188950.025.670799
GTACATG96850.024.5292131
TACATGG96200.024.3388522
GAGTACT115300.024.05535512-13
AGTACTT115400.023.7258512-13
ACATGGG98350.023.1790223
GTACTTT122200.022.65820914-15
CGTCACT1903.2758726E-722.5061991
GTATATG2354.629328E-922.2401681
CTAATAC3251.8189894E-1221.9197793
Produced by FastQC (version 0.11.5)