##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936993_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 821866 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11451623500668 31.0 31.0 34.0 31.0 34.0 2 32.19607089233525 33.0 31.0 34.0 30.0 34.0 3 32.24799419856765 34.0 31.0 34.0 30.0 34.0 4 35.97984099597745 37.0 35.0 37.0 35.0 37.0 5 35.91727726904386 37.0 35.0 37.0 35.0 37.0 6 35.99577060007349 37.0 35.0 37.0 35.0 37.0 7 35.942018528567914 37.0 35.0 37.0 35.0 37.0 8 35.915292760620346 37.0 35.0 37.0 35.0 37.0 9 37.543421920361716 39.0 37.0 39.0 35.0 39.0 10-11 37.46621955403922 39.0 37.0 39.0 35.0 39.0 12-13 37.349546641423295 39.0 37.0 39.0 34.0 39.0 14-15 38.36938868380004 40.0 38.0 41.0 33.5 41.0 16-17 38.33442130468957 40.0 38.0 41.0 33.5 41.0 18-19 38.61485753151973 40.0 38.0 41.0 34.0 41.0 20-21 38.6923975441252 40.0 38.0 41.0 34.0 41.0 22-23 38.584651269185 40.0 38.0 41.0 34.0 41.0 24-25 38.449317771996895 40.0 38.0 41.0 34.0 41.0 26-27 38.21219943397099 40.0 37.5 41.0 34.0 41.0 28-29 38.04139482592053 40.0 37.0 41.0 33.0 41.0 30-31 37.773616380285844 39.5 36.5 41.0 33.0 41.0 32-33 37.33968615321719 39.0 35.0 41.0 33.0 41.0 34-35 37.04803763630568 39.0 35.0 41.0 33.0 41.0 36-37 36.75426651059905 39.0 35.0 41.0 32.0 41.0 38-39 36.47236593313265 39.0 35.0 41.0 31.0 41.0 40-41 36.10699493591413 39.0 35.0 41.0 30.5 41.0 42-43 35.58505279449448 39.0 35.0 41.0 29.5 41.0 44-45 34.87925647246631 38.0 35.0 40.0 25.0 41.0 46-47 33.92659448133881 38.0 34.0 40.0 14.5 41.0 48-49 33.08877724592573 38.0 33.0 40.0 2.0 41.0 50-51 32.58136302024904 38.0 33.0 40.0 2.0 41.0 52-53 32.2569390873938 38.0 33.0 40.0 2.0 41.0 54-55 31.925511579746576 37.0 32.0 40.0 2.0 41.0 56-57 31.601259816077075 37.0 31.5 40.0 2.0 41.0 58-59 31.22696218118282 36.0 31.0 40.0 2.0 41.0 60-61 30.87420029542529 36.0 31.0 39.0 2.0 41.0 62-63 30.488958053989336 35.0 30.0 39.0 2.0 40.0 64-65 30.093666120754477 35.0 29.5 38.5 2.0 40.0 66-67 29.703103668967934 35.0 29.0 38.0 2.0 40.0 68-69 29.40124971710717 35.0 29.0 37.5 2.0 40.0 70-71 28.9673906695252 34.5 28.5 37.0 2.0 39.0 72-73 28.506238851588947 34.0 27.5 36.0 2.0 39.0 74-75 28.016019643104837 34.0 26.0 36.0 2.0 38.5 76-77 26.699752757748833 32.5 25.0 34.5 2.0 36.5 78-79 27.386835566868562 33.5 26.0 35.0 2.0 37.0 80-81 27.17833503271823 34.0 26.0 35.0 2.0 37.0 82-83 26.883703669454633 33.5 25.0 35.0 2.0 36.0 84-85 26.56358092438427 33.0 25.0 35.0 2.0 36.0 86-87 26.21449238683678 33.0 24.0 35.0 2.0 35.0 88-89 25.840517797305157 33.0 22.5 35.0 2.0 35.0 90-91 25.401987671956256 33.0 19.5 35.0 2.0 35.0 92-93 24.959035292857962 32.0 17.5 35.0 2.0 35.0 94-95 24.413658674285102 32.0 5.0 34.0 2.0 35.0 96-97 23.80919699805078 32.0 2.0 34.0 2.0 35.0 98-99 23.085399566352667 31.0 2.0 34.0 2.0 35.0 100-101 20.11019862118642 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09321965494178386 1101 2 0.0961385526143701 1101 3 0.11048078213767809 1101 4 0.14882399590818807 1101 5 0.20206941610453555 1101 6 0.17867753080476234 1101 7 0.1667659316373573 1101 8 0.24054046505344218 1101 9 0.20368525105136115 1101 10-11 0.19454675776555774 1101 12-13 0.21010708636267594 1101 14-15 0.3510250668836008 1101 16-17 0.23480687504869735 1101 18-19 0.08390887235569977 1101 20-21 0.17356320932267977 1101 22-23 0.2004654627439777 1101 24-25 0.2611743176451924 1101 26-27 0.2326909096331633 1101 28-29 0.24493750035079387 1101 30-31 0.5119630182116097 1101 32-33 0.5417717733436476 1101 34-35 0.6690555601687294 1101 36-37 0.6293614578393942 1101 38-39 0.6255406509996675 1101 40-41 0.556928074224551 1101 42-43 0.7388808720786173 1101 44-45 0.9448283319686297 1101 46-47 0.6671898651044614 1101 48-49 0.9657222089451309 1101 50-51 0.6964390052458 1101 52-53 0.822948139338564 1101 54-55 0.6777700154018902 1101 56-57 0.8473563609841932 1101 58-59 0.7332203744698234 1101 60-61 0.7778532738792805 1101 62-63 0.7226258235097944 1101 64-65 0.6907295718177942 1101 66-67 0.5585421446690226 1101 68-69 0.6892144597495609 1101 70-71 0.6908167832024823 1101 72-73 0.6585900633500685 1101 74-75 0.6317533279701344 1101 76-77 0.6335379016365827 1101 78-79 0.8369770685607563 1101 80-81 0.9250557805760273 1101 82-83 0.8771068113261506 1101 84-85 0.6004780196460224 1101 86-87 0.2812413034466097 1101 88-89 0.4419432289765659 1101 90-91 0.5350667768049924 1101 92-93 0.9251192944790709 1101 94-95 0.8342913298158727 1101 96-97 0.45130636059201024 1101 98-99 0.8382720908621089 1101 100-101 0.9582743642500553 1102 1 0.11657787794796803 1102 2 0.12570391649577317 1102 3 0.16513979196802353 1102 4 0.10811897569254825 1102 5 0.07825980971481528 1102 6 0.07562734846767682 1102 7 0.02078973455474653 1102 8 0.05657448479340843 1102 9 0.11299608623448876 1102 10-11 0.13210931226849 1102 12-13 0.10494181860462248 1102 14-15 0.1535051980870321 1102 16-17 0.2156261780057278 1102 18-19 0.1361363081413387 1102 20-21 0.04837554581387593 1102 22-23 0.06859004158495452 1102 24-25 0.15968056566326538 1102 26-27 -0.09597292618418152 1102 28-29 0.11917260123076545 1102 30-31 0.018298364850267035 1102 32-33 0.1684365902137941 1102 34-35 0.19793220516635301 1102 36-37 0.08690924700223235 1102 38-39 -0.010834358909953323 1102 40-41 -0.13641922342092982 1102 42-43 -0.24502404012211088 1102 44-45 -0.5400780716305533 1102 46-47 -0.460176057679746 1102 48-49 -0.34118071142112427 1102 50-51 -0.10675771565954761 1102 52-53 -0.06014244949130898 1102 54-55 -0.09449071672114684 1102 56-57 -0.34303786864162333 1102 58-59 -0.08984452365924156 1102 60-61 -0.20553200240104275 1102 62-63 0.059828039301770275 1102 64-65 0.06067380540745404 1102 66-67 -0.0802966455640508 1102 68-69 0.0025412940210038926 1102 70-71 -0.1326965254228547 1102 72-73 0.0137949696050228 1102 74-75 -0.13881781322218956 1102 76-77 -0.2623614958582685 1102 78-79 -0.04294429857100468 1102 80-81 0.3449494750821316 1102 82-83 0.10076083661550683 1102 84-85 0.05212772433921842 1102 86-87 0.007851493513204133 1102 88-89 -0.006092559631330374 1102 90-91 0.3443467682827155 1102 92-93 0.00990603936570622 1102 94-95 -0.055690163960886 1102 96-97 -0.014686286466812248 1102 98-99 -0.0432756933459153 1102 100-101 0.5378814724235177 1103 1 0.12800123255092188 1103 2 0.16226181188521593 1103 3 0.0710967851106048 1103 4 0.08497321942563474 1103 5 0.1299135034930501 1103 6 0.07694266928074711 1103 7 -0.057531420107281406 1103 8 0.05591682438687684 1103 9 0.14495886824742144 1103 10-11 0.2722347418447342 1103 12-13 0.23599615399684382 1103 14-15 0.29540363635629774 1103 16-17 0.3087848078839315 1103 18-19 0.24030923028090712 1103 20-21 0.21671776749041527 1103 22-23 0.2322429379043669 1103 24-25 0.22479320064471864 1103 26-27 0.17274699436168817 1103 28-29 0.1266809921752312 1103 30-31 0.08163191294639205 1103 32-33 -0.01668934385241272 1103 34-35 -0.038873558904967354 1103 36-37 -0.20354993611303485 1103 38-39 -0.26851324314835523 1103 40-41 -0.1127325080395778 1103 42-43 0.017528946519234978 1103 44-45 -0.05381600186019142 1103 46-47 -0.37538709859449426 1103 48-49 -0.4543943367832384 1103 50-51 -0.7033559945586134 1103 52-53 -0.5610164935436188 1103 54-55 -0.4679512625671336 1103 56-57 -0.5348492064152772 1103 58-59 -0.5686164370620155 1103 60-61 -0.7668372577276656 1103 62-63 -0.8669850550694598 1103 64-65 -0.8377620325868378 1103 66-67 -0.6848518859991053 1103 68-69 -0.7698814612721883 1103 70-71 -0.7781324716089735 1103 72-73 -0.7330477134063109 1103 74-75 -0.4750020213492192 1103 76-77 -0.6135576233203892 1103 78-79 -0.7131336828865891 1103 80-81 -0.526310765152342 1103 82-83 -0.6698354218924436 1103 84-85 -0.5839867061402408 1103 86-87 -0.8544677258524054 1103 88-89 -0.7552230742232453 1103 90-91 -0.7801048268172899 1103 92-93 -0.8837134390524568 1103 94-95 -0.8803519429505222 1103 96-97 -0.7961436667902468 1103 98-99 -0.8895849542012613 1103 100-101 -0.7462509203298175 1104 1 0.15633969462432162 1104 2 0.0873091875350056 1104 3 0.18966398319587796 1104 4 0.0021391414108293816 1104 5 0.12438257748019765 1104 6 0.11379657842381619 1104 7 0.08090283639926099 1104 8 -0.009600077274605212 1104 9 0.04668293623654307 1104 10-11 0.1930545289295722 1104 12-13 0.12387610091294476 1104 14-15 0.01191296370900119 1104 16-17 0.19908018854075493 1104 18-19 0.13867904431337053 1104 20-21 0.02109545138616653 1104 22-23 0.17196430533657292 1104 24-25 0.16829650679862596 1104 26-27 5.893884691445805E-4 1104 28-29 -0.06869097187142614 1104 30-31 0.04089406979890953 1104 32-33 -0.007831401259529969 1104 34-35 0.055166671279842205 1104 36-37 0.1747111602203546 1104 38-39 0.28991730443882346 1104 40-41 0.033205355970572725 1104 42-43 0.13339558768708315 1104 44-45 0.12977376847656075 1104 46-47 0.2793426428822414 1104 48-49 0.15811225524142714 1104 50-51 0.4312893690024069 1104 52-53 0.32759016976184085 1104 54-55 0.18622735667172918 1104 56-57 0.18328972738630966 1104 58-59 0.392587107274057 1104 60-61 0.45701167335018056 1104 62-63 0.4377878605468304 1104 64-65 0.36737887737334773 1104 66-67 0.3763000811769608 1104 68-69 0.48086872562257454 1104 70-71 0.36514714034533924 1104 72-73 0.5924400964188479 1104 74-75 0.7774791877584946 1104 76-77 0.6559585365572183 1104 78-79 0.5047763410475277 1104 80-81 0.8687355094120051 1104 82-83 1.0763875652944037 1104 84-85 0.8777018953073998 1104 86-87 0.6349160719651259 1104 88-89 0.740814756754343 1104 90-91 0.8821360559055229 1104 92-93 0.8703573897171673 1104 94-95 0.5929594845777757 1104 96-97 0.43710331826396853 1104 98-99 0.4293931226081398 1104 100-101 0.6886604422923845 1105 1 0.21194590874117836 1105 2 0.2014766985110228 1105 3 0.18024711952369188 1105 4 0.10101339560423384 1105 5 0.1809400468036273 1105 6 0.04414105664063328 1105 7 0.18419564288355872 1105 8 0.07497720517501705 1105 9 0.24234881488114723 1105 10-11 0.11512758983242577 1105 12-13 0.2859215810435316 1105 14-15 0.25315747569818825 1105 16-17 0.24865803386328622 1105 18-19 0.08093109879947491 1105 20-21 0.18708667056888117 1105 22-23 0.2035358464825805 1105 24-25 0.12146829560871453 1105 26-27 0.2931059932197897 1105 28-29 0.4182728916881828 1105 30-31 0.42752865271009455 1105 32-33 0.30879304993938916 1105 34-35 0.30720145682526834 1105 36-37 0.3464943415779942 1105 38-39 0.4491681206045328 1105 40-41 0.3359578044677036 1105 42-43 0.34666250201782844 1105 44-45 0.712034828929113 1105 46-47 0.5429782389646434 1105 48-49 0.7118159589451309 1105 50-51 0.9029430895922985 1105 52-53 0.7453431811318723 1105 54-55 0.7334073634262097 1105 56-57 0.756933203689357 1105 58-59 0.6280959443786394 1105 60-61 0.8675711690160597 1105 62-63 1.0119970241176972 1105 64-65 0.777020890206849 1105 66-67 0.7467070382860435 1105 68-69 0.9102844635489511 1105 70-71 0.8681866540231518 1105 72-73 0.846301404535474 1105 74-75 1.137599642559799 1105 76-77 0.8993813666821744 1105 78-79 1.0512105412051334 1105 80-81 1.0266016582356023 1105 82-83 1.0636047216604965 1105 84-85 1.0271165074879676 1105 86-87 0.6839507449359736 1105 88-89 0.7170643345996659 1105 90-91 0.8013637942821958 1105 92-93 1.1462439145398626 1105 94-95 0.9207963640103998 1105 96-97 0.9496441265494582 1105 98-99 0.9530422276402213 1105 100-101 0.8577305762561345 1106 1 0.13669487299929983 1106 2 0.15275001733226645 1106 3 0.14397274740563404 1106 4 0.09738771388712308 1106 5 0.10037922476397654 1106 6 0.09594336453099572 1106 7 0.10695972780268903 1106 8 0.10857269238331213 1106 9 0.009352536529910083 1106 10-11 0.06422116940314737 1106 12-13 0.16482638641767977 1106 14-15 0.26245516078584785 1106 16-17 0.15039246262530526 1106 18-19 -0.07830455880884557 1106 20-21 0.07272204261672499 1106 22-23 0.17193811224107236 1106 24-25 0.1171047209533782 1106 26-27 -0.003863437765865285 1106 28-29 0.10916538519284558 1106 30-31 0.16558368161723536 1106 32-33 0.30373546971328125 1106 34-35 0.09693941998328626 1106 36-37 0.13975909358511274 1106 38-39 0.3213909279916507 1106 40-41 0.27383084709111927 1106 42-43 0.03052482442027582 1106 44-45 -0.14883694973811856 1106 46-47 0.11310030436267482 1106 48-49 0.01462123947317906 1106 50-51 -0.14247038792566258 1106 52-53 -0.13791876208372855 1106 54-55 -0.19439499127734905 1106 56-57 -0.30700835004048344 1106 58-59 -0.04171013197367657 1106 60-61 -0.1114518396677866 1106 62-63 -0.041147652052046624 1106 64-65 -0.29064406777830953 1106 66-67 -0.07769960450589508 1106 68-69 -0.11111618715441907 1106 70-71 -0.007491552095334697 1106 72-73 -0.30917339826395107 1106 74-75 -0.12593639189470807 1106 76-77 -0.050327806362790994 1106 78-79 0.12344154090084558 1106 80-81 0.1484253831612854 1106 82-83 0.19166337859001814 1106 84-85 0.11646767664743507 1106 86-87 -0.2182559118999272 1106 88-89 -0.2715536229407718 1106 90-91 -0.23916940797419883 1106 92-93 0.011281482126410225 1106 94-95 -0.12530234160484355 1106 96-97 -0.17161374391843864 1106 98-99 -0.5567926388251472 1106 100-101 -0.19289437985649371 1107 1 0.012774837683672047 1107 2 0.031885064594959545 1107 3 0.015872994755206093 1107 4 0.01696406642683712 1107 5 0.049115499665347784 1107 6 0.01646567153876788 1107 7 0.027884941343145897 1107 8 0.12384109820479239 1107 9 0.040054014321000864 1107 10-11 0.1404528371559124 1107 12-13 0.057945793899826015 1107 14-15 0.09371965666218784 1107 16-17 0.13598242289521778 1107 18-19 -0.08029106319176549 1107 20-21 -0.04724950867854005 1107 22-23 0.016132404281748336 1107 24-25 -0.11695944979491202 1107 26-27 -0.023211710172724054 1107 28-29 0.07764408019610869 1107 30-31 0.02376233387737159 1107 32-33 0.19171869478738301 1107 34-35 0.051183252009799673 1107 36-37 0.1780930225194055 1107 38-39 -0.02354611348319935 1107 40-41 0.07551149286270231 1107 42-43 -0.05069938080279002 1107 44-45 0.10577720594496753 1107 46-47 0.01659367917786625 1107 48-49 0.3694592710470417 1107 50-51 0.3176085817547474 1107 52-53 0.1944826309218115 1107 54-55 0.30853046050349775 1107 56-57 0.3560444202046966 1107 58-59 0.35089600762117357 1107 60-61 0.4531428363603247 1107 62-63 0.5567411177166477 1107 64-65 0.48811995512079775 1107 66-67 0.22779639095352522 1107 68-69 0.6077163648905959 1107 70-71 0.5072005222076363 1107 72-73 0.2087647012784828 1107 74-75 0.23778485284123008 1107 76-77 0.2603008679696117 1107 78-79 0.5005182613117434 1107 80-81 0.6759929488350487 1107 82-83 0.6965473064732493 1107 84-85 0.7791560792456558 1107 86-87 0.7256332222364179 1107 88-89 0.7977200241691662 1107 90-91 0.9719921540418746 1107 92-93 0.8855378568084653 1107 94-95 1.1362715864886042 1107 96-97 0.7789005750293789 1107 98-99 0.35650362710717687 1107 100-101 0.5459381021178196 1108 1 0.020708327649671787 1108 2 0.005053797816927386 1108 3 -0.06299336498386054 1108 4 0.03936570699560349 1108 5 -0.021428051912522506 1108 6 -0.008022895634468341 1108 7 0.06185521735164201 1108 8 0.007781269957490622 1108 9 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2316 86-87 -1.916479158882158 2316 88-89 -2.0262215554874494 2316 90-91 -2.00315060061401 2316 92-93 -2.035578683342191 2316 94-95 -2.17142407706606 2316 96-97 -2.2015427642177308 2316 98-99 -2.4732596279898154 2316 100-101 -2.6464486393920374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 12.0 5 22.0 6 88.0 7 196.0 8 466.0 9 912.0 10 1723.0 11 3083.0 12 4823.0 13 6934.0 14 8688.0 15 12400.0 16 17797.0 17 25357.0 18 21025.0 19 9500.0 20 6450.0 21 5824.0 22 6096.0 23 6494.0 24 7307.0 25 8192.0 26 9526.0 27 11450.0 28 13941.0 29 17148.0 30 20846.0 31 25450.0 32 31978.0 33 41306.0 34 57235.0 35 79422.0 36 113409.0 37 151321.0 38 90895.0 39 4547.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.59020236184152 19.48525339957427 15.932511163553203 17.992033075031006 2 16.654782619276492 22.416679848630494 39.56170984266819 21.36682768942482 3 19.19183807559636 28.96181246311742 31.819946098203477 20.026403363082746 4 12.296803364060233 18.544065684833498 37.3847741232126 31.77435682789367 5 11.733056240311681 40.1994972416428 31.74167078331504 16.32577573473048 6 27.062319161517813 38.182015073016764 17.852666979775293 16.902998785690123 7 25.30947867413909 38.085040627060856 19.5643815415165 17.041099157283547 8 28.255944399695327 31.4483139587232 19.424456054879993 20.871285586701482 9 30.047233977315035 14.829789771081902 20.321317587051904 34.801658664551155 10-11 25.230863026442847 27.904840076738342 25.15511038285812 21.709186513960695 12-13 28.068018387425685 26.876522450131773 26.837343313873554 18.21811584856899 14-15 24.576816658676716 25.604356427933507 29.367013600757303 20.45181331263247 16-17 19.80803439976833 29.01677402398931 29.072257521299093 22.102934054943262 18-19 19.63319920716713 24.819404207270555 36.4984188362294 19.04897774933292 20-21 22.028986917984774 23.163921550495314 36.824142015578204 17.98294951594171 22-23 21.294783790015593 23.770109320236077 36.54595751178893 18.3891493779594 24-25 20.193387091180455 24.164563993762258 37.21679323266869 18.425255682388595 26-27 19.891277233927344 24.355846510467686 37.119546369925104 18.633329885679867 28-29 19.72374924257357 24.49030372495626 37.009558782374654 18.776388250095515 30-31 20.017716408484894 24.341627354329386 36.89521158088526 18.745444656300457 32-33 19.443768434783454 24.578372774440993 36.75755886962532 19.220299921150232 34-35 19.901601964310483 24.507523124207594 36.759702919940715 18.8311719915412 36-37 20.319043368798145 24.409452172394637 36.068972944163455 19.202531514643763 38-39 20.165474909827246 24.84131542696372 35.764813399597934 19.228396263611096 40-41 20.252295112884877 25.079728175894772 35.44883221492842 19.219144496291925 42-43 20.134060783631394 25.342634930755136 34.81705046808117 19.7062538175323 44-45 20.70075900450925 25.51967108993437 34.04873787211054 19.730832033445843 46-47 21.00987265563973 25.989212353352002 32.65526253671523 20.345652454293038 48-49 21.340363113366763 26.554170717551045 31.296108595743437 20.80935757333875 50-51 21.58775915483703 27.20453489022957 30.334774873875343 20.87293108105806 52-53 21.686138616270778 27.50107681787542 29.69851532974962 21.11426923610418 54-55 21.596110170465987 27.38066194512162 29.495379549800415 21.527848334611978 56-57 21.983454079534308 27.769537400496937 28.7365818401283 21.510426679840457 58-59 21.998117692293185 27.364763126723595 29.049349435763965 21.587769745219248 60-61 22.057965530227214 27.344206762665475 29.543451029110525 21.05437667799679 62-63 22.322122808362447 27.329624478442284 29.500789105117075 20.847463608078197 64-65 22.117501049442424 27.494357345792803 29.60942490555018 20.77871669921459 66-67 22.34408233192674 27.840867609911367 28.91832269871168 20.896727359450214 68-69 22.533364725407566 27.63621939652996 29.032193302172445 20.798222575890037 70-71 22.210838766668836 27.7892524902492 29.09927596761231 20.900632775469656 72-73 22.11680492927071 28.100748783865008 28.722991339220748 21.059454947643534 74-75 21.56227414201342 28.355778192552073 28.83024726658604 21.251700398848474 76-77 21.215623958163494 28.429999537637524 29.10103350181173 21.25334300238725 78-79 21.041664638716647 28.302435486854016 28.921528068450613 21.73437180597872 80-81 20.996961115048716 28.390121038270483 28.738543342895124 21.874374503785678 82-83 21.107369809603313 28.38950153208122 28.441219921193344 22.06190873712212 84-85 20.961534015339666 28.04137548354752 28.40010416273724 22.596986338375572 86-87 20.724982265123927 28.21773925655886 28.070688843272368 22.986589635044847 88-89 20.899856363183954 27.959475071620687 28.268406775082756 22.872261790112603 90-91 20.664699179483623 27.903214718312903 28.62280155817198 22.8092845440315 92-93 20.375086388988933 27.82394654103355 28.917679810771613 22.883287259205904 94-95 20.225693863533632 28.101617600446787 28.72313048789613 22.949558048123446 96-97 20.25846754371876 28.19184662782718 28.49123434617762 23.058451482276443 98-99 20.019577769922876 27.899173876514038 28.49983056529138 23.58141778827171 100-101 20.10184185310803 28.491627249083635 28.39501741471353 23.0115134830948 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2527.0 1 1709.5 2 928.0 3 944.5 4 794.0 5 770.0 6 1180.5 7 2370.0 8 3841.5 9 4285.5 10 3532.0 11 2769.0 12 2677.5 13 2780.0 14 2881.0 15 2972.0 16 3142.0 17 3360.0 18 3563.0 19 3860.5 20 4191.5 21 4556.5 22 4932.0 23 5405.0 24 5970.5 25 6849.5 26 8354.5 27 9959.5 28 10676.5 29 11603.5 30 12997.5 31 13835.0 32 14829.0 33 15977.5 34 17811.0 35 19319.0 36 20385.0 37 21173.0 38 20136.5 39 19438.5 40 19938.5 41 20615.5 42 21309.0 43 22892.5 44 24833.0 45 25536.0 46 25646.0 47 25910.5 48 28195.0 49 28437.5 50 26043.0 51 24451.5 52 22675.0 53 21693.0 54 20851.5 55 20054.0 56 19542.0 57 18597.0 58 17874.0 59 17515.5 60 16430.5 61 14753.5 62 12433.5 63 8518.0 64 5277.5 65 3780.5 66 2640.0 67 1848.0 68 1422.5 69 1072.0 70 732.0 71 562.0 72 446.0 73 405.0 74 319.0 75 231.5 76 180.5 77 83.5 78 33.0 79 23.0 80 15.0 81 9.5 82 6.5 83 4.0 84 1.5 85 0.5 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026646679629039282 2 0.004380276103403718 3 1.2167433620565883E-4 4 2.4334867241131765E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013688362823136617 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.6502300861697645E-4 20-21 0.0015209292025707354 22-23 0.001764277874982053 24-25 0.01149822477143476 26-27 0.0062053911464886 28-29 4.866973448226353E-4 30-31 0.003528555749964106 32-33 0.006083716810282942 34-35 0.0 36-37 0.007969669021470654 38-39 0.01338417698262247 40-41 0.0013384176982622472 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.003528555749964106 50-51 0.00730046017233953 52-53 0.0 54-55 0.0037110672542725945 56-57 0.0038935787585810825 58-59 3.041858405141471E-4 60-61 0.001885952211187712 62-63 0.0059620424740772825 64-65 1.2167433620565883E-4 66-67 0.0026768353965244945 68-69 3.041858405141471E-4 70-71 0.0012775805301594178 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.002190138051701859 80-81 0.002250975219804688 82-83 0.013140828310211154 84-85 0.009125575215424413 86-87 0.0047452991120206945 88-89 1.8251150430848823E-4 90-91 6.692088491311236E-4 92-93 2.4334867241131765E-4 94-95 6.0837168102829414E-5 96-97 0.0026768353965244945 98-99 0.001885952211187712 100-101 4.2586017671980585E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 821866.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.25749206766298 #Duplication Level Percentage of deduplicated Percentage of total 1 73.3257713234462 35.385178279964755 2 14.095638909445121 13.604403657223793 3 5.284921351021301 7.651111505253995 4 2.471619717936601 4.770966757304198 5 1.327418681187955 3.20289482389477 6 0.7909154925955425 2.2900558866072696 7 0.5267329559858152 1.7793168011683502 8 0.36605829439804394 1.4132044190572677 9 0.26663552306480603 1.1580445475331345 >10 1.3661965534893807 12.049263257289965 >50 0.10256064206264956 3.4832911173035845 >100 0.06921529154094255 6.39310807701206 >500 0.004294379401440846 1.4853943618167107 >1k 0.0012630527651296608 1.2654804160869326 >5k 2.5261055302593213E-4 0.9295143216128371 >10k+ 5.052211060518643E-4 3.1387717708704694 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14413 1.7536922077321608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11335 1.3791786008911429 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7625 0.9277668135681486 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.33849800332414287 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2563 0.3118513236951036 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2310 0.2810677166350719 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1710 0.20806311491167662 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1016 0.12362112558494938 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 994 0.12094429018842487 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 934 0.11364383001608534 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.11364383001608534 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 919 0.11181871497300047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 874 0.10634336984374582 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 869 0.10573499816271753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.0837168102829414E-5 0.0 16-17 0.0 0.0 0.0 2.4334867241131765E-4 0.0 18-19 0.0 0.0 0.0 3.650230086169765E-4 0.0 20-21 0.0 0.0 0.0 6.083716810282942E-4 0.0 22-23 0.0 0.0 0.0 0.0010950690258509296 0.0 24-25 0.0 0.0 0.0 0.002190138051701859 0.0 26-27 0.0 0.0 0.0 0.0035893929180669357 0.0 28-29 0.0 0.0 0.0 0.008213017693881972 0.0 30-31 0.0 0.0 0.0 0.017277755741203556 0.0 32-33 0.0 0.0 0.0 0.0268900283014506 0.0 34-35 0.0 0.0 0.0 0.03394713980137881 0.0 36-37 0.0 0.0 0.0 0.03911829909011931 0.0 38-39 0.0 0.0 0.0 0.045323690236607914 0.0 40-41 0.0 0.0 0.0 0.05396256810720969 0.0 42-43 0.0 0.0 0.0 0.06284479465022279 0.0 44-45 0.0 0.0 0.0 0.07270041588288115 0.0 46-47 0.0 0.0 0.0 0.08322524596467064 0.0 48-49 0.0 0.0 0.0 0.09514933091282521 0.0 50-51 0.0 0.0 0.0 0.11388717868849665 0.0 52-53 0.0 0.0 0.0 0.1534921751234386 0.0 54-55 0.0 0.0 0.0 0.20240525827811345 0.0 56-57 0.0 0.0 0.0 0.2583146157646137 0.0 58-59 0.0 0.0 0.0 0.3322317750095515 0.0 60-61 0.0 0.0 0.0 0.44113030591361607 0.0 62-63 0.0 0.0 0.0 0.5092070970206821 0.0 64-65 0.0 0.0 0.0 0.5671240810545758 0.0 66-67 0.0 0.0 0.0 0.7058328243290268 0.0 68-69 0.0 0.0 0.0 0.8818955888186153 0.0 70-71 0.0 0.0 0.0 0.9931059321105874 0.0 72-73 0.0 0.0 0.0 1.116666220527434 0.0 74-75 0.0 0.0 0.0 1.3754066964687675 0.0 76-77 0.0 0.0 0.0 1.5386328184886588 0.0 78-79 0.0 0.0 0.0 1.6580561794745128 0.0 80-81 0.0 0.0 0.0 1.8344231298046154 0.0 82-83 0.0 0.0 0.0 2.1465178021721303 0.0 84-85 0.0 0.0 0.0 2.4575174055137943 0.0 86-87 0.0 0.0 0.0 2.9125185857548557 0.0 88-89 0.0 0.0 0.0 3.4762966225637757 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6400 0.0 49.59178 1 GTATCAA 11590 0.0 42.79867 1 TATCAAC 15380 0.0 32.207443 2 ATCAACG 15470 0.0 31.958668 3 TCAACGC 15670 0.0 31.58108 4 CAACGCA 15990 0.0 30.958477 5 AACGCAG 16405 0.0 30.204277 6 CATGGGT 1150 0.0 27.669748 4 ACGCAGA 18360 0.0 26.858704 7 CGCAGAG 18365 0.0 26.825523 8 GCAGAGT 18895 0.0 25.67079 9 GTACATG 9685 0.0 24.529213 1 TACATGG 9620 0.0 24.338852 2 GAGTACT 11530 0.0 24.055355 12-13 AGTACTT 11540 0.0 23.72585 12-13 ACATGGG 9835 0.0 23.179022 3 GTACTTT 12220 0.0 22.658209 14-15 CGTCACT 190 3.2758726E-7 22.506199 1 GTATATG 235 4.629328E-9 22.240168 1 CTAATAC 325 1.8189894E-12 21.919779 3 >>END_MODULE