FastQCFastQC Report
Thu 26 May 2016
SRR936992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837197
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144111.7213391830118836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104421.2472572166407667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69760.8332566886885643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22560.26947062638781555No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.2596760380173364No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.20401410898510147No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.1828721316488234No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11090.13246583540074797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9800.11705727564718937No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9550.11407112065618964No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.10750157967599024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53400.052.862151
GTATCAA104700.046.2970281
TATCAAC136500.035.3393862
ATCAACG137050.035.1628843
TCAACGC138900.034.696634
CAACGCA141300.034.10735
AACGCAG144600.033.2572146
TAGCGCC753.0670344E-431.685485
ACGCAGA162000.029.5971457
CGCAGAG162050.029.5000388
ATAGCGT650.00583135129.242896
GCAGAGT166050.028.1009069
AATAGCG700.008366018527.1589815
CGTCACT1754.9312803E-927.1557351
TAGCGTA700.00837334127.154117
GGACGTC700.00837334127.154116
GTACATG96400.026.8176981
CATGGGT11800.026.583584
TACATGG95900.026.4635562
GAGTACT101300.025.5628212-13
Produced by FastQC (version 0.11.5)