##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936992_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 837197 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750038521399382 33.0 31.0 34.0 30.0 34.0 2 31.77025120730246 33.0 31.0 34.0 30.0 34.0 3 31.725689413602773 33.0 31.0 34.0 30.0 34.0 4 35.41723274211446 37.0 35.0 37.0 33.0 37.0 5 35.45067648355166 37.0 35.0 37.0 33.0 37.0 6 35.56351253050357 37.0 35.0 37.0 33.0 37.0 7 35.5227598761104 37.0 35.0 37.0 33.0 37.0 8 35.49091790821037 37.0 35.0 37.0 33.0 37.0 9 37.063375764604984 39.0 37.0 39.0 33.0 39.0 10-11 36.928809467783566 39.0 37.0 39.0 33.0 39.0 12-13 36.82576502304714 39.0 37.0 39.0 32.5 39.0 14-15 37.777234629364415 40.0 37.0 41.0 32.5 41.0 16-17 37.767965007041354 40.0 37.0 41.0 32.5 41.0 18-19 37.89321270859786 40.0 37.5 41.0 33.0 41.0 20-21 37.90612185662395 40.0 37.5 41.0 33.0 41.0 22-23 37.78065556852211 40.0 37.0 41.0 33.0 41.0 24-25 37.680754350529206 40.0 37.0 41.0 32.0 41.0 26-27 37.62772680743004 40.0 37.0 41.0 32.0 41.0 28-29 37.50194757028513 40.0 37.0 41.0 32.0 41.0 30-31 37.15360661827503 39.0 36.0 41.0 32.0 41.0 32-33 36.730745571233534 39.0 35.0 41.0 31.0 41.0 34-35 36.43962711285396 39.0 35.0 41.0 30.5 41.0 36-37 36.142459301693634 38.0 35.0 40.5 30.0 41.0 38-39 35.868667709033836 38.0 35.0 40.0 30.0 41.0 40-41 35.52989678653889 38.0 35.0 40.0 28.5 41.0 42-43 35.06911933511468 38.0 35.0 40.0 26.5 41.0 44-45 34.430542632140344 38.0 34.5 40.0 23.5 41.0 46-47 33.54577895047402 38.0 33.5 40.0 13.5 41.0 48-49 32.83210642178603 38.0 33.0 40.0 2.0 41.0 50-51 31.926369181924922 37.0 32.0 39.5 2.0 40.5 52-53 32.01213752557642 37.5 32.5 39.5 2.0 40.5 54-55 32.305920828669954 38.0 33.0 40.0 2.0 41.0 56-57 32.14471325148084 37.0 32.5 40.0 2.0 41.0 58-59 31.896042389067325 37.0 32.0 40.0 2.0 41.0 60-61 31.595874686603032 36.5 31.5 40.0 2.0 41.0 62-63 31.258891276485702 36.0 31.0 39.5 2.0 41.0 64-65 30.921912644216356 35.0 31.0 39.0 2.0 41.0 66-67 30.546681366512303 35.0 30.5 39.0 2.0 41.0 68-69 30.141291715092148 35.0 30.0 38.5 2.0 40.0 70-71 29.74179434470023 35.0 29.5 37.5 2.0 40.0 72-73 29.37816547359821 35.0 29.0 37.0 2.0 39.0 74-75 29.010904243565136 35.0 29.0 36.5 2.0 39.0 76-77 28.6064695645111 35.0 28.5 36.0 2.0 39.0 78-79 28.195157770512793 34.0 27.5 35.5 2.0 37.5 80-81 27.798217743255172 34.0 26.5 35.0 2.0 37.0 82-83 27.43507621264768 34.0 26.0 35.0 2.0 36.5 84-85 27.104779998017193 34.0 26.0 35.0 2.0 36.0 86-87 26.76404896338616 34.0 25.0 35.0 2.0 36.0 88-89 26.426810535632594 34.0 24.5 35.0 2.0 35.5 90-91 26.065269584100278 33.5 23.0 35.0 2.0 35.0 92-93 25.708516633480528 33.0 20.0 35.0 2.0 35.0 94-95 25.346686622145086 33.0 18.5 35.0 2.0 35.0 96-97 24.891860577617933 33.0 7.5 35.0 2.0 35.0 98-99 24.403761002488064 33.0 2.0 35.0 2.0 35.0 100-101 22.52128411831385 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25462291708558027 1101 2 0.1724150597387144 1101 3 0.4831317834742954 1101 4 0.4188123686381289 1101 5 0.3052251724110633 1101 6 0.4079508589409002 1101 7 0.3283518822108249 1101 8 0.4886055554634581 1101 9 0.4660802453828694 1101 10-11 0.40192626914433305 1101 12-13 0.5815892410281478 1101 14-15 0.6769244642425605 1101 16-17 0.7120207762907214 1101 18-19 0.5043116993890067 1101 20-21 0.6659508730264676 1101 22-23 0.5691832391934639 1101 24-25 0.5223723281134554 1101 26-27 0.6525316196503752 1101 28-29 0.761694166379506 1101 30-31 0.715244366537938 1101 32-33 0.6844529054006117 1101 34-35 0.665832730685473 1101 36-37 0.774908672510513 1101 38-39 0.9117727047760056 1101 40-41 0.9821473501561542 1101 42-43 0.8455506948249223 1101 44-45 1.1311333625967208 1101 46-47 1.0633183066086147 1101 48-49 0.9940795507079443 1101 50-51 1.1529176633677487 1101 52-53 1.2296516441705556 1101 54-55 1.2327670222526521 1101 56-57 1.1898408817329695 1101 58-59 1.0554302293521438 1101 60-61 1.1300564443036407 1101 62-63 1.254969973967789 1101 64-65 1.1611037103867865 1101 66-67 1.2756610738693404 1101 68-69 1.1814366932916052 1101 70-71 0.9930604560631586 1101 72-73 1.1211944043872748 1101 74-75 1.2296549810942636 1101 76-77 1.4189932209386065 1101 78-79 1.3065861746980403 1101 80-81 1.3077247730558348 1101 82-83 1.3638677515008837 1101 84-85 0.9798435665826624 1101 86-87 1.0622622160212387 1101 88-89 1.0841934386115568 1101 90-91 1.1986636097977268 1101 92-93 1.4045759795427308 1101 94-95 1.2638151725320554 1101 96-97 1.4854273664827247 1101 98-99 1.4537832830940793 1101 100-101 1.5424872412507185 1102 1 0.2964350602943 1102 2 0.21430885381293052 1102 3 0.2642798636439423 1102 4 0.21751423487791044 1102 5 0.21224898686350002 1102 6 0.0918228579840914 1102 7 0.13774656179442957 1102 8 0.10064894312336747 1102 9 0.15255817204889155 1102 10-11 0.36730917720316114 1102 12-13 0.2515794429242888 1102 14-15 0.06499147775290481 1102 16-17 0.2801267982145532 1102 18-19 0.047318705263265315 1102 20-21 -0.02541460544760099 1102 22-23 0.09400625231741344 1102 24-25 -0.1118139692612985 1102 26-27 0.11153023503569415 1102 28-29 0.07332101962415294 1102 30-31 0.2593525090842519 1102 32-33 0.26978438491428847 1102 34-35 0.16679241585813287 1102 36-37 0.37950458122714537 1102 38-39 0.6279945467275283 1102 40-41 0.3792902598709986 1102 42-43 0.476411731951913 1102 44-45 0.5298471889632808 1102 46-47 0.4802230257986366 1102 48-49 1.0806501683564917 1102 50-51 0.9369528491407202 1102 52-53 1.2573652129343849 1102 54-55 0.8416156760483844 1102 56-57 1.1000669051288199 1102 58-59 0.7458199224828839 1102 60-61 0.7408325185300271 1102 62-63 0.5326375422440144 1102 64-65 0.7618551283518151 1102 66-67 0.5246042126666097 1102 68-69 0.664567164474601 1102 70-71 0.49449912131300877 1102 72-73 0.7258058382684709 1102 74-75 0.9363564471298034 1102 76-77 0.7598683342148043 1102 78-79 0.5151219101768341 1102 80-81 0.5933527257121334 1102 82-83 0.6369778214676245 1102 84-85 0.5714914880506292 1102 86-87 0.7576107617158243 1102 88-89 0.7096683936460835 1102 90-91 0.49697562190505806 1102 92-93 0.3231177410854045 1102 94-95 0.05212840365230775 1102 96-97 -0.18637007957020302 1102 98-99 -0.1075922924870234 1102 100-101 -0.029305144748573042 1103 1 0.2094519097014711 1103 2 0.24483217736016272 1103 3 0.16592619252359952 1103 4 0.1956452887813711 1103 5 0.2478850503200576 1103 6 0.2959296929451085 1103 7 0.3672939378537592 1103 8 0.2021048756991064 1103 9 0.19843302975095867 1103 10-11 0.3039724486909847 1103 12-13 0.2264434693401185 1103 14-15 0.449969808523214 1103 16-17 0.5820613844354554 1103 18-19 0.47456867697010807 1103 20-21 0.3900753987464398 1103 22-23 0.463526533518106 1103 24-25 0.5377370016932801 1103 26-27 0.628053438753092 1103 28-29 0.7419498999474285 1103 30-31 0.640398091024565 1103 32-33 0.6910628361801656 1103 34-35 0.6952769677943849 1103 36-37 0.749537467861451 1103 38-39 0.8211818873704786 1103 40-41 0.767517310792023 1103 42-43 0.9383807359662057 1103 44-45 0.9535971307126587 1103 46-47 0.7781749340888666 1103 48-49 0.6345486325373528 1103 50-51 0.7954271489682974 1103 52-53 0.6951069529968272 1103 54-55 0.9790372041451505 1103 56-57 0.8794784740343076 1103 58-59 0.824107088041135 1103 60-61 0.7025601985577197 1103 62-63 0.850902272993018 1103 64-65 0.9887812266256013 1103 66-67 1.021493629694433 1103 68-69 0.9975493297998419 1103 70-71 0.8958941403859235 1103 72-73 0.6294593169953444 1103 74-75 0.896822585213723 1103 76-77 1.0579583796835728 1103 78-79 1.1116282045721277 1103 80-81 1.047569404694645 1103 82-83 0.9369501940319154 1103 84-85 0.9537104038568529 1103 86-87 0.9616893035197975 1103 88-89 1.0255148916189611 1103 90-91 1.009795750655563 1103 92-93 0.9941016106572782 1103 94-95 1.1120266814311925 1103 96-97 0.9846420831587182 1103 98-99 0.9688391748293874 1103 100-101 0.9680585987356807 1104 1 0.16834530687727423 1104 2 0.22630119517323521 1104 3 0.06828438980708995 1104 4 0.24129650121142276 1104 5 0.29115819481813077 1104 6 0.18498417057140415 1104 7 0.04054355806798071 1104 8 0.189688925337272 1104 9 0.2224316452154511 1104 10-11 0.2863260308561095 1104 12-13 0.027648848516953706 1104 14-15 0.04272567517192982 1104 16-17 0.2038556398950746 1104 18-19 0.1561639695493824 1104 20-21 0.18749093246564996 1104 22-23 0.09747553646023732 1104 24-25 0.15679423557080696 1104 26-27 0.1237299702352459 1104 28-29 0.19324277407648793 1104 30-31 0.35472546957323203 1104 32-33 0.21564795626939315 1104 34-35 0.11591455921539051 1104 36-37 0.03729810987569948 1104 38-39 0.23808251489838028 1104 40-41 0.31059976116348054 1104 42-43 0.3289912135668658 1104 44-45 0.41235111285471504 1104 46-47 0.20982791564762238 1104 48-49 0.3676649912089687 1104 50-51 0.468891500383922 1104 52-53 0.6730986073244587 1104 54-55 0.5429469785812415 1104 56-57 0.516376024816644 1104 58-59 0.41656809990583454 1104 60-61 0.3293349359126161 1104 62-63 0.23160787331094923 1104 64-65 0.44442641376224046 1104 66-67 0.0349656088751189 1104 68-69 0.12379685398113693 1104 70-71 0.0752732146701014 1104 72-73 0.07453940852852625 1104 74-75 0.11101856108690811 1104 76-77 0.20573190556990184 1104 78-79 0.15216112254480763 1104 80-81 0.25474495225920535 1104 82-83 0.2508984277876607 1104 84-85 0.514702077414146 1104 86-87 0.38901881656070714 1104 88-89 0.5475793237991553 1104 90-91 0.38600000810225055 1104 92-93 0.4386112222822476 1104 94-95 0.6217729140203367 1104 96-97 0.6968622114846497 1104 98-99 0.6800513484860353 1104 100-101 0.650490381517443 1105 1 0.07028066223632123 1105 2 0.12072123488478681 1105 3 -0.019877796065856046 1105 4 -0.06585772327962758 1105 5 0.15084652059694292 1105 6 -0.00867321240772867 1105 7 0.14656534644397823 1105 8 0.218104722651546 1105 9 -0.07157475016665416 1105 10-11 -0.08062544010295625 1105 12-13 -8.17276936793121E-4 1105 14-15 0.13153841846875736 1105 16-17 0.08604806079513594 1105 18-19 -0.004648739842167515 1105 20-21 0.03469046287762012 1105 22-23 0.11560479183687278 1105 24-25 -0.030732650251060534 1105 26-27 0.15696667354080063 1105 28-29 0.17535933192841213 1105 30-31 0.07861364150686967 1105 32-33 -0.016985127224465657 1105 34-35 -0.22779537693674 1105 36-37 -0.149942170436141 1105 38-39 -0.09554854911265664 1105 40-41 0.037529342030730106 1105 42-43 0.43017143492591003 1105 44-45 0.22866929688830595 1105 46-47 0.3552365059723286 1105 48-49 0.7480387120795697 1105 50-51 0.498095065523362 1105 52-53 0.6608466190980629 1105 54-55 0.43383822106115844 1105 56-57 0.702281285007821 1105 58-59 0.654106322803333 1105 60-61 0.6956326708284735 1105 62-63 0.4948892044319635 1105 64-65 0.744312195374139 1105 66-67 0.5899175799369694 1105 68-69 0.7481211324875225 1105 70-71 0.6222961198010282 1105 72-73 0.5799250492216359 1105 74-75 0.5184677614522357 1105 76-77 0.4328866386400456 1105 78-79 0.5223780951006098 1105 80-81 0.49470316401034964 1105 82-83 0.4774110797521516 1105 84-85 0.43909967607319444 1105 86-87 0.36865658110130184 1105 88-89 0.22470841600666347 1105 90-91 0.25837049525744504 1105 92-93 0.3578382668231832 1105 94-95 0.5131398898607138 1105 96-97 0.19789685207816277 1105 98-99 0.4532049414913786 1105 100-101 0.795817387279218 1106 1 0.24212244232390745 1106 2 0.24494440000989925 1106 3 0.39827288264251237 1106 4 0.34769781814247835 1106 5 0.2243732600710615 1106 6 0.11981800163526657 1106 7 0.22810120804925305 1106 8 0.3281835872914769 1106 9 0.3847203380563471 1106 10-11 0.40838302785146396 1106 12-13 0.5082219464100532 1106 14-15 0.38614718443702856 1106 16-17 0.5331471958281142 1106 18-19 0.4821711889252498 1106 20-21 0.3188621115847141 1106 22-23 0.0846960767490117 1106 24-25 0.3005613022484397 1106 26-27 0.12888136707716313 1106 28-29 0.13786682770352598 1106 30-31 0.07223428260007836 1106 32-33 0.26000647750741024 1106 34-35 0.563984483505557 1106 36-37 0.32306849627897805 1106 38-39 0.3513972632476552 1106 40-41 0.589490963662179 1106 42-43 0.7690974493541489 1106 44-45 0.7898723006498187 1106 46-47 1.0780098064756771 1106 48-49 0.6936693566253709 1106 50-51 0.7191397948577425 1106 52-53 0.8839776725043258 1106 54-55 1.0341362349078977 1106 56-57 0.9279552233334911 1106 58-59 0.9898988760914769 1106 60-61 1.0029390072570337 1106 62-63 1.1329704677199288 1106 64-65 0.8767784416716786 1106 66-67 0.7365122265906763 1106 68-69 0.7485822362290513 1106 70-71 0.7545829217853672 1106 72-73 0.8093923040416442 1106 74-75 0.7513705798636785 1106 76-77 0.4853411642710519 1106 78-79 0.5689893233174317 1106 80-81 0.778139999612101 1106 82-83 0.8602975437451761 1106 84-85 0.7318233227450683 1106 86-87 0.839048269422431 1106 88-89 1.07378666281301 1106 90-91 1.1692355077049754 1106 92-93 1.1822474634578057 1106 94-95 1.30873237409687 1106 96-97 1.3798261283042876 1106 98-99 1.3391544517674028 1106 100-101 1.6697043782479462 1107 1 0.3000346117443371 1107 2 0.42926712751514273 1107 3 0.32088017713327943 1107 4 0.21458998653984196 1107 5 0.20931110855435975 1107 6 0.3830693188381886 1107 7 0.33405036145863676 1107 8 0.051097802852375196 1107 9 0.37195114270108576 1107 10-11 0.4955089677972424 1107 12-13 0.3599042575257059 1107 14-15 0.4446041387569366 1107 16-17 0.4745924104170953 1107 18-19 0.6298481800756974 1107 20-21 0.4531733295498199 1107 22-23 0.5776093971234602 1107 24-25 0.5456320219783493 1107 26-27 0.45315306895449936 1107 28-29 0.639028012418855 1107 30-31 0.38548240270851863 1107 32-33 0.3802261818401149 1107 34-35 0.22017853435214363 1107 36-37 0.4374579791169779 1107 38-39 0.42825413572195004 1107 40-41 0.466193096763611 1107 42-43 0.7331179874890879 1107 44-45 0.8767981024811107 1107 46-47 0.7053635074322528 1107 48-49 0.7656218861665209 1107 50-51 0.5947402092011878 1107 52-53 0.6950818210541279 1107 54-55 0.8012777766747448 1107 56-57 0.7266744672845888 1107 58-59 0.7832335735184621 1107 60-61 0.7956943911641474 1107 62-63 0.6170770716382883 1107 64-65 0.5592864983497705 1107 66-67 0.5049274907985613 1107 68-69 0.5149986769677106 1107 70-71 0.39776287983948677 1107 72-73 0.33926915196225593 1107 74-75 0.3480187460102826 1107 76-77 0.47917332959920245 1107 78-79 0.42938691398703455 1107 80-81 0.5355988210351939 1107 82-83 0.5824994466558095 1107 84-85 0.3168340649825261 1107 86-87 0.2669640195886771 1107 88-89 0.07891608119758331 1107 90-91 0.5220368018888237 1107 92-93 0.5791840075265569 1107 94-95 0.41092685974831156 1107 96-97 0.24176280025681862 1107 98-99 0.7632036358688943 1107 100-101 0.7029656703856517 1108 1 -3.3784574955220137E-4 1108 2 0.023969282447787066 1108 3 0.0317382805023243 1108 4 0.1672770013172098 1108 5 0.08459468414629612 1108 6 -0.021691606984724388 1108 7 0.06890174124435333 1108 8 -0.00943258629314414 1108 9 -0.09394722961129531 1108 10-11 -0.19916779230626958 1108 12-13 -0.009497996567695566 1108 14-15 0.1880087388488434 1108 16-17 0.18799362453178503 1108 18-19 0.015308431121724198 1108 20-21 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-2.556725797867035 2316 84-85 -2.4520190008063274 2316 86-87 -2.6877626345827785 2316 88-89 -2.592223922897489 2316 90-91 -3.0130138352093496 2316 92-93 -3.06258175454996 2316 94-95 -3.3652807917298553 2316 96-97 -3.277066640136308 2316 98-99 -3.6747513498771163 2316 100-101 -3.7252312394125866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1291.0 3 436.0 4 466.0 5 489.0 6 703.0 7 900.0 8 1152.0 9 1524.0 10 2128.0 11 3279.0 12 5347.0 13 7377.0 14 8660.0 15 11776.0 16 16552.0 17 23020.0 18 18877.0 19 8105.0 20 6775.0 21 6336.0 22 6529.0 23 7189.0 24 7733.0 25 8544.0 26 9909.0 27 11553.0 28 13650.0 29 16289.0 30 19525.0 31 23967.0 32 29958.0 33 39016.0 34 53943.0 35 77602.0 36 107084.0 37 143619.0 38 117579.0 39 18310.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.11883606330149 18.744772274293688 15.317535046673136 18.81885661573169 2 16.17052646419606 21.555006744639236 40.708467897548395 21.56599889361631 3 18.745086607136457 29.114048062241487 31.279405685557194 20.861459645064862 4 11.881097010814035 17.80258442152864 38.1991507953675 32.117167772289825 5 11.799399096372937 39.57594807312708 31.988015403983944 16.63663742651604 6 26.88143112530119 37.78371688400208 18.60080237723688 16.73404961345985 7 25.48760899495181 37.5902315664678 19.877595800826068 17.04456363775432 8 27.63636537516691 31.24010036807205 19.975111204620088 21.14842305214095 9 30.029475818115316 14.817276224035341 20.493343437506724 34.659904520342614 10-11 24.861418820265513 27.551701612532103 25.73238099051701 21.854498576685373 12-13 27.75165426762799 26.595579376159424 26.837802883971772 18.814963472240816 14-15 24.390609751797736 25.453326345367657 29.216788089542373 20.939275813292234 16-17 19.763676724099298 29.011117053219042 28.777781429352483 22.44742479332917 18-19 19.602750638387388 25.194205420600625 35.711148308460174 19.491895632551813 20-21 22.22198983165096 23.57280982510153 35.718776069242296 18.486424274005213 22-23 21.282631099427956 24.174092273684526 35.5127925559721 19.030484070915417 24-25 20.042826296660206 24.564853169116088 36.27542624708443 19.116894287139267 26-27 19.88419317462403 24.772985176248554 36.10813813204008 19.23468351708733 28-29 19.709256453153305 24.95993750070927 35.94712646012355 19.383679586013873 30-31 20.069032411427916 24.787879729030763 35.843278866334664 19.299808993206657 32-33 19.33029082966459 25.12000688057367 35.66693484180355 19.882767447958198 34-35 19.842964959182357 25.15439509375993 35.696981859621715 19.305658087436004 36-37 20.131613447315498 24.88136812047086 35.09156380259894 19.895454629614697 38-39 20.00826595346937 25.39535028172328 34.771055784435404 19.82532798037194 40-41 20.05740978654095 25.596809133961546 34.47167407582607 19.874107003671433 42-43 19.820335820672668 25.81043583584277 34.03627446178986 20.332953881694703 44-45 20.334242540252863 25.996250491981083 33.27249391257399 20.397013055192065 46-47 20.68164186030678 26.271210229047483 32.12708787608944 20.9200600345563 48-49 20.774194704324277 26.918898580672906 30.969579875596114 21.337326839406707 50-51 21.177421262170366 27.519329843031997 30.011574613253828 21.291674281543806 52-53 21.35079024352951 27.829282073518748 29.29527311212965 21.52465457082209 54-55 21.201708278580814 27.60297455501135 29.26818779118385 21.927129375223988 56-57 21.566457340607386 28.072368601807838 28.421716553323193 21.93945750426158 58-59 21.597330972969292 27.615005034885847 28.817345331054195 21.970318661090666 60-61 21.611055235565196 27.62214248343841 29.245930972863597 21.5208713081328 62-63 21.93222155127463 27.555757835304068 29.27342348718739 21.23859712623391 64-65 21.729169416485053 27.773056219327703 29.384245735516945 21.1135286286703 66-67 21.77586808376423 27.99671690863735 28.919040974127135 21.308374033471285 68-69 22.02390092605483 27.768201288315 28.94382905956351 21.26406872606666 70-71 21.671349674261066 27.981876700852027 28.965786356851154 21.380987268035753 72-73 21.55794948304664 28.22712859897905 28.69698846522995 21.51793345274435 74-75 21.001878559631574 28.562528559183587 28.76185299449573 21.673739886689106 76-77 20.82366863057011 28.47823269000213 28.96621182081507 21.731886858612693 78-79 20.693478425799313 28.317941751539905 28.871118725757437 22.117461096903344 80-81 20.513522414233126 28.49308862352827 28.667793111156403 22.325595851082205 82-83 20.615804996478364 28.579954227722755 28.36519132417609 22.43904945162279 84-85 20.650237902113105 28.16419367131401 28.27570842028935 22.90986000628353 86-87 20.416390608300212 28.423865542001398 27.95972903365333 23.20001481604506 88-89 20.52000430200043 27.898114289811428 28.27615257761526 23.305728830572882 90-91 20.235746642561057 27.810073929975037 28.60015796901083 23.354021458453076 92-93 20.276495217883824 27.629446937868334 29.005171663870527 23.08888618037731 94-95 20.017823406122197 27.947447233101364 28.76765840223677 23.267070958539676 96-97 19.91715099783171 27.909839737654785 28.57842580922628 23.594583455287225 98-99 19.720082018143405 27.707829482559195 28.73269780424621 23.83939069505119 100-101 19.9693992206364 27.90420521647661 28.74817710201057 23.378218460876425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1680.0 1 1151.5 2 711.5 3 795.0 4 715.5 5 758.0 6 1236.5 7 2446.5 8 3962.0 9 4434.5 10 3581.5 11 2785.0 12 2707.5 13 2833.5 14 2939.0 15 3069.0 16 3233.0 17 3407.0 18 3628.5 19 3836.5 20 4095.0 21 4413.0 22 4854.0 23 5346.5 24 5812.0 25 6737.5 26 8233.5 27 9695.5 28 10392.5 29 11472.5 30 13160.0 31 14307.5 32 15330.0 33 16590.0 34 18319.0 35 19816.0 36 21019.5 37 21822.5 38 20846.0 39 20010.0 40 20426.5 41 21071.0 42 21755.5 43 23206.5 44 24877.5 45 25346.0 46 25470.0 47 26066.5 48 28741.0 49 29205.5 50 27024.0 51 25608.0 52 23897.5 53 22859.5 54 21782.5 55 20736.5 56 20068.0 57 19047.0 58 18242.0 59 17488.0 60 16483.5 61 15318.0 62 12884.5 63 8705.0 64 5405.5 65 3920.5 66 2694.5 67 1861.0 68 1422.5 69 1045.5 70 766.0 71 583.0 72 459.5 73 422.0 74 323.5 75 247.0 76 188.5 77 86.5 78 34.0 79 23.0 80 15.5 81 10.0 82 6.5 83 3.5 84 3.0 85 3.0 86 3.5 87 2.5 88 2.0 89 2.5 90 1.5 91 1.0 92 1.0 93 1.0 94 2.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037386660487316606 2 0.02818930311503744 3 0.023769793728357844 4 0.04945072665095551 5 0.09436249771559144 6 0.11132385806446989 7 0.055064698034035006 8 0.08074563095663267 9 0.06915934959155372 10-11 0.07089131948633356 12-13 0.0684426723937138 14-15 0.06217174691261436 16-17 0.050107680748975446 18-19 0.06193285451333438 20-21 0.057692514426114755 22-23 0.033982443797576915 24-25 0.011287665865978975 26-27 0.003225047390279707 28-29 0.008062618475699267 30-31 0.006450094780559414 32-33 0.0068681564792993765 34-35 0.00704732577875936 36-37 0.005793140682539474 38-39 0.003344493589919696 40-41 0.0068681564792993765 42-43 0.00286670879135974 44-45 0.0024486470926197777 46-47 0.0014930774954998644 48-49 5.375078983799512E-4 50-51 0.002388923992799783 52-53 0.005972309981999458 54-55 0.011586281365078948 56-57 0.007883449176239284 58-59 0.004538955586319588 60-61 0.002269477793159794 62-63 0.008659849473899214 64-65 0.0024486470926197777 66-67 0.021619762134838037 68-69 0.043896478367696015 70-71 0.05781196062575475 72-73 0.0038820014882996476 74-75 0.014273820856978705 76-77 0.02072391563753812 78-79 0.025501763623137684 80-81 0.07662473706905304 82-83 0.027174010418097534 84-85 0.010511265568319047 86-87 0.03183241220405711 88-89 0.04503121726427591 90-91 0.03870056868335649 92-93 0.03929779968155643 94-95 0.011407112065618964 96-97 0.01576689835247857 98-99 0.03690887568875665 100-101 0.0736983051778733 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 837197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.945025560959536 #Duplication Level Percentage of deduplicated Percentage of total 1 74.50109463917838 37.2095907607323 2 13.634602741540347 13.619611648795232 3 4.990503053938804 7.477524077730605 4 2.340762104254131 4.67637692516392 5 1.2568940227384946 3.1387802046545685 6 0.7370730756793255 2.208788016305938 7 0.49993413761830274 1.7478456297499678 8 0.3471164975373246 1.386939387370594 9 0.24384314840421914 1.0960877051922218 >10 1.2824258288785073 11.674308601630013 >50 0.09594885592110154 3.3329438717768824 >100 0.06356529894602352 6.051591745338596 >500 0.004317642945036487 1.5147848277923461 >1k 0.0011993452625101353 1.0516277458368566 >5k 2.39869052502027E-4 0.8357433532647427 >10k+ 4.79738105004054E-4 2.977455498665231 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14411 1.7213391830118836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10442 1.2472572166407667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6976 0.8332566886885643 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2256 0.26947062638781555 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2174 0.2596760380173364 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1708 0.20401410898510147 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1531 0.1828721316488234 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1109 0.13246583540074797 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 980 0.11705727564718937 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 955 0.11407112065618964 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.10750157967599024 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1944619963998916E-4 0.0 18-19 0.0 0.0 0.0 1.1944619963998916E-4 0.0 20-21 0.0 0.0 0.0 2.3889239927997832E-4 0.0 22-23 0.0 0.0 0.0 8.361233974799241E-4 0.0 24-25 0.0 0.0 0.0 0.001970862294059821 0.0 26-27 0.0 0.0 0.0 0.0036431090890196695 0.0 28-29 0.0 0.0 0.0 0.007644556776959306 0.0 30-31 0.0 0.0 0.0 0.016901637249058465 0.0 32-33 0.0 0.0 0.0 0.025860102222057653 0.0 34-35 0.0 0.0 0.0 0.033265766599736984 0.0 36-37 0.0 0.0 0.0 0.038163060784976534 0.0 38-39 0.0 0.0 0.0 0.04329924736949607 0.0 40-41 0.0 0.0 0.0 0.05022712694861544 0.0 42-43 0.0 0.0 0.0 0.05811057612485472 0.0 44-45 0.0 0.0 0.0 0.06533707120307407 0.0 46-47 0.0 0.0 0.0 0.07387747447733328 0.0 48-49 0.0 0.0 0.0 0.08791240293503202 0.0 50-51 0.0 0.0 0.0 0.10732241037653026 0.0 52-53 0.0 0.0 0.0 0.15510089023252593 0.0 54-55 0.0 0.0 0.0 0.20425300138438146 0.0 56-57 0.0 0.0 0.0 0.26361776260545605 0.0 58-59 0.0 0.0 0.0 0.33212015809898987 0.0 60-61 0.0 0.0 0.0 0.4319174578982008 0.0 62-63 0.0 0.0 0.0 0.49528366680721503 0.0 64-65 0.0 0.0 0.0 0.5527970119338698 0.0 66-67 0.0 0.0 0.0 0.6925490655126572 0.0 68-69 0.0 0.0 0.0 0.8711211339744409 0.0 70-71 0.0 0.0 0.0 0.9812505300425108 0.0 72-73 0.0 0.0 0.0 1.1019509147787199 0.0 74-75 0.0 0.0 0.0 1.3684353861755358 0.0 76-77 0.0 0.0 0.0 1.521207075515082 0.0 78-79 0.0 0.0 0.0 1.6336059493763115 0.0 80-81 0.0 0.0 0.0 1.7977250276816568 0.0 82-83 0.0 0.0 0.0 2.101237820966869 0.0 84-85 0.0 0.0 0.0 2.4070200920452414 0.0 86-87 0.0 0.0 0.0 2.8443723520270616 0.0 88-89 0.0 0.0 0.0 3.4181918950975696 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5340 0.0 52.86215 1 GTATCAA 10470 0.0 46.297028 1 TATCAAC 13650 0.0 35.339386 2 ATCAACG 13705 0.0 35.162884 3 TCAACGC 13890 0.0 34.69663 4 CAACGCA 14130 0.0 34.1073 5 AACGCAG 14460 0.0 33.257214 6 TAGCGCC 75 3.0670344E-4 31.68548 5 ACGCAGA 16200 0.0 29.597145 7 CGCAGAG 16205 0.0 29.500038 8 ATAGCGT 65 0.005831351 29.24289 6 GCAGAGT 16605 0.0 28.100906 9 AATAGCG 70 0.0083660185 27.158981 5 CGTCACT 175 4.9312803E-9 27.155735 1 TAGCGTA 70 0.008373341 27.15411 7 GGACGTC 70 0.008373341 27.15411 6 GTACATG 9640 0.0 26.817698 1 CATGGGT 1180 0.0 26.58358 4 TACATGG 9590 0.0 26.463556 2 GAGTACT 10130 0.0 25.56282 12-13 >>END_MODULE