FastQCFastQC Report
Thu 26 May 2016
SRR936992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837197
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143211.7105890250442846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115311.3773341280487148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78050.9322775881901153No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.3477078871520084No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.3026766698877325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23930.28583475573849404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.21201700436098073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10780.1287630032119083No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.12709075641694845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9780.11681838324790939No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.11251832006086979No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9330.11144330426410987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9110.10881548787203012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.049.002881
GTATCAA121400.040.189751
TATCAAC161050.030.3210352
ATCAACG161250.030.2539713
TCAACGC162800.029.9075684
GTGTAAG1751.5097612E-1029.861951
CAACGCA165400.029.4087185
AACGCAG169500.028.7270976
ACGCAGA189900.025.5675687
CGCAGAG189800.025.5089978
GCAGAGT193550.024.5483469
GAGTACT116400.024.53002512-13
AGTACTT116850.023.31745712-13
GTACATG102450.023.0466581
GTACTTT124000.022.98345414-15
TACATGG101550.022.9674722
GATCGTC1050.002193672822.622697
GTACACG1302.937385E-421.9266071
ACATGGG102250.021.648823
CATGGGT12050.021.287194
Produced by FastQC (version 0.11.5)