##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 837197 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.035473132369084 31.0 31.0 34.0 30.0 34.0 2 32.116974857769435 33.0 31.0 34.0 30.0 34.0 3 32.19063613462542 33.0 31.0 34.0 30.0 34.0 4 35.95005596054453 37.0 35.0 37.0 35.0 37.0 5 35.817978325292614 37.0 35.0 37.0 35.0 37.0 6 35.94021837154218 37.0 35.0 37.0 35.0 37.0 7 35.88301916992058 37.0 35.0 37.0 35.0 37.0 8 35.855078314900794 37.0 35.0 37.0 35.0 37.0 9 37.47555951586067 39.0 37.0 39.0 35.0 39.0 10-11 37.404893352460654 39.0 37.0 39.0 34.0 39.0 12-13 37.280250645905326 39.0 37.0 39.0 34.0 39.0 14-15 38.27300384497316 40.0 37.5 41.0 33.5 41.0 16-17 38.31231239481269 40.0 37.5 41.0 33.5 41.0 18-19 38.56631354388513 40.0 38.0 41.0 34.0 41.0 20-21 38.62529548003636 40.0 38.0 41.0 34.0 41.0 22-23 38.50087315171937 40.0 38.0 41.0 34.0 41.0 24-25 38.37416462314127 40.0 38.0 41.0 34.0 41.0 26-27 38.11642540525109 40.0 37.0 41.0 33.5 41.0 28-29 37.90291592062561 40.0 37.0 41.0 33.0 41.0 30-31 37.60313880723414 39.0 36.5 41.0 33.0 41.0 32-33 37.195176284673735 39.0 35.0 41.0 33.0 41.0 34-35 36.927527212830434 39.0 35.0 41.0 32.5 41.0 36-37 36.63493060773032 39.0 35.0 41.0 31.5 41.0 38-39 36.33674451771805 39.0 35.0 41.0 31.0 41.0 40-41 35.8310206558313 38.5 35.0 40.5 29.5 41.0 42-43 35.360907289443226 38.0 35.0 40.0 28.0 41.0 44-45 34.706880817776465 38.0 35.0 40.0 24.5 41.0 46-47 33.73780006378427 38.0 33.5 40.0 14.0 41.0 48-49 32.888899506328855 38.0 33.0 40.0 2.0 41.0 50-51 32.39075868642625 38.0 33.0 40.0 2.0 41.0 52-53 32.059739822287945 37.5 32.0 40.0 2.0 41.0 54-55 31.61583952164186 37.0 31.0 40.0 2.0 41.0 56-57 31.38762023753071 36.5 31.0 40.0 2.0 41.0 58-59 31.045538266381747 36.0 31.0 39.0 2.0 41.0 60-61 30.65136939095577 35.5 30.0 39.0 2.0 40.0 62-63 30.25161640569663 35.0 29.5 39.0 2.0 40.0 64-65 29.926210318479402 35.0 29.0 38.0 2.0 40.0 66-67 29.60509593321524 35.0 29.0 38.0 2.0 40.0 68-69 29.1464828469285 35.0 28.5 37.0 2.0 39.5 70-71 28.72525522666708 34.0 28.0 37.0 2.0 39.0 72-73 28.343610285273357 34.0 26.5 36.0 2.0 39.0 74-75 27.886205994526975 34.0 26.0 36.0 2.0 38.5 76-77 26.533560201481848 32.0 24.5 34.5 2.0 36.5 78-79 27.147957410263057 33.0 26.0 35.0 2.0 37.0 80-81 27.011424431764567 33.0 25.5 35.0 2.0 36.5 82-83 26.688259752483585 33.0 25.0 35.0 2.0 36.0 84-85 26.28525902505623 33.0 24.0 35.0 2.0 36.0 86-87 25.92540107047684 33.0 23.0 35.0 2.0 35.0 88-89 25.554406549474017 33.0 20.5 35.0 2.0 35.0 90-91 25.105920709223753 32.0 18.5 34.5 2.0 35.0 92-93 24.64716966257643 32.0 14.0 34.0 2.0 35.0 94-95 24.122968668067372 31.5 4.5 34.0 2.0 35.0 96-97 23.421972367316172 31.0 2.0 34.0 2.0 35.0 98-99 22.6630386874296 31.0 2.0 34.0 2.0 35.0 100-101 19.97987928767064 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.153818254410794 1101 2 0.22713145730367046 1101 3 0.1376386344901448 1101 4 0.19234480731270764 1101 5 -0.05652040916650236 1101 6 0.008201620858578451 1101 7 0.08935402822117311 1101 8 0.18627475939127436 1101 9 0.10542475006425178 1101 10-11 0.10999943786773514 1101 12-13 -0.029439927523178255 1101 14-15 0.20869502154192077 1101 16-17 0.23807863642158367 1101 18-19 0.08799425027521579 1101 20-21 -0.038136801755072725 1101 22-23 -0.008074357428021983 1101 24-25 0.09648442989585249 1101 26-27 0.0692919279177957 1101 28-29 0.1163359993513211 1101 30-31 0.35101711503155997 1101 32-33 0.2817752482321012 1101 34-35 0.09178898201935226 1101 36-37 0.11384330308049329 1101 38-39 0.05234920653577291 1101 40-41 0.2124130128446069 1101 42-43 0.5487921670825102 1101 44-45 0.1512482036554843 1101 46-47 0.29502139777155634 1101 48-49 0.6255852307998282 1101 50-51 0.5796690560288624 1101 52-53 0.5320806373804601 1101 54-55 0.2673363822300274 1101 56-57 0.30620545284186207 1101 58-59 0.5179356356264613 1101 60-61 0.4928355929015069 1101 62-63 0.5813558516237194 1101 64-65 0.7095706421208128 1101 66-67 0.6083452316473803 1101 68-69 0.38260764708360995 1101 70-71 0.1849988412440986 1101 72-73 0.23276388917264512 1101 74-75 0.07140887080255354 1101 76-77 0.1424961953383317 1101 78-79 0.11755675935760834 1101 80-81 0.34182782720196414 1101 82-83 0.595415080092188 1101 84-85 0.33246780276222765 1101 86-87 0.19464782903039435 1101 88-89 0.4341990190903964 1101 90-91 0.7819733590168134 1101 92-93 0.553265704587087 1101 94-95 0.3401099044553 1101 96-97 0.561742262709533 1101 98-99 0.5515375611180566 1101 100-101 0.983773861684746 1102 1 -0.022145110800877887 1102 2 0.16247892198679637 1102 3 0.1523081899874299 1102 4 0.0185779653841891 1102 5 -0.03160079501859059 1102 6 0.11545060769783788 1102 7 0.06474951741336099 1102 8 0.16802636595830478 1102 9 0.279527395552293 1102 10-11 0.3249050908676523 1102 12-13 0.24791368742513953 1102 14-15 0.21517419022111284 1102 16-17 0.1062211137318414 1102 18-19 0.03105369670534941 1102 20-21 0.03627588701942841 1102 22-23 0.05411657679587023 1102 24-25 -0.02459000349314522 1102 26-27 0.09001227255345867 1102 28-29 -0.010671921932640771 1102 30-31 -0.06265836992858453 1102 32-33 -0.10082230730198205 1102 34-35 0.1469843920906868 1102 36-37 0.03429695988925374 1102 38-39 0.31777489504806766 1102 40-41 0.43371905868868765 1102 42-43 0.38923717579960737 1102 44-45 0.4971086197758936 1102 46-47 0.41830845461436184 1102 48-49 0.6433540691495168 1102 50-51 0.7287767615705363 1102 52-53 0.511692646267754 1102 54-55 0.7820306629894027 1102 56-57 0.4904466057980841 1102 58-59 0.5163601189259843 1102 60-61 0.5897994457183415 1102 62-63 0.47017094806373905 1102 64-65 0.6907869180133375 1102 66-67 0.7261225800872744 1102 68-69 0.8366054344601572 1102 70-71 0.47511759150285116 1102 72-73 0.5339716319747225 1102 74-75 0.46814467620209044 1102 76-77 0.2833537134280206 1102 78-79 0.0911317792528159 1102 80-81 0.6330873895533209 1102 82-83 0.49444860984760197 1102 84-85 0.6470133869454209 1102 86-87 0.6421100971304341 1102 88-89 0.3378406476916638 1102 90-91 0.3893724224468862 1102 92-93 0.37896698202062495 1102 94-95 0.4168594180001435 1102 96-97 0.7263245322815912 1102 98-99 0.8680743914084523 1102 100-101 1.004801834581258 1103 1 0.032793542977422874 1103 2 0.20859343937210895 1103 3 0.12868173954705497 1103 4 0.13206348261638112 1103 5 0.049587251288983225 1103 6 0.07771752201284698 1103 7 0.002910661921490032 1103 8 0.09013031168870356 1103 9 0.13946628233306058 1103 10-11 0.16562793110119145 1103 12-13 0.2803026950343366 1103 14-15 0.13885256692619663 1103 16-17 0.1565213540572401 1103 18-19 0.12394845281833256 1103 20-21 0.13320824307383816 1103 22-23 0.18687472688940687 1103 24-25 0.17285795374091606 1103 26-27 0.2569635350939805 1103 28-29 0.05686883685653754 1103 30-31 0.08226583769658902 1103 32-33 0.0718791233331828 1103 34-35 0.11498649577013964 1103 36-37 0.001659971080037792 1103 38-39 0.21315012888268825 1103 40-41 0.20389374396892634 1103 42-43 -0.182474327199472 1103 44-45 -0.2779607816740466 1103 46-47 -0.35258520971562035 1103 48-49 -0.33490846471905655 1103 50-51 -0.5062578226531969 1103 52-53 -0.7616542012088274 1103 54-55 -0.45198869618723236 1103 56-57 -0.4241280315502145 1103 58-59 -0.5379427709703641 1103 60-61 -0.5942375550738639 1103 62-63 -0.25871403525994197 1103 64-65 -0.2033251095741555 1103 66-67 -0.3185356718616248 1103 68-69 -0.22016894591970626 1103 70-71 -0.27344112179093827 1103 72-73 -0.06080545123379011 1103 74-75 -0.3926147121331418 1103 76-77 -0.3101102344326847 1103 78-79 -0.37701179021187414 1103 80-81 -0.2934352815869907 1103 82-83 -0.24087722746341456 1103 84-85 -0.46358289966093125 1103 86-87 -0.4840291185166805 1103 88-89 -0.3361739182913084 1103 90-91 -0.7471021170403738 1103 92-93 -0.741355493397613 1103 94-95 -0.5753682853277944 1103 96-97 -0.5460437048217202 1103 98-99 -0.5507591982275954 1103 100-101 -0.2107920221421935 1104 1 0.06255785144680459 1104 2 0.1622499591882729 1104 3 0.13289944493585182 1104 4 0.07510523020786763 1104 5 -0.12850183331510578 1104 6 0.03951459782257416 1104 7 0.17256113080469504 1104 8 0.05526052853516461 1104 9 0.0044933000051230465 1104 10-11 0.12058565789900655 1104 12-13 0.12228777281831071 1104 14-15 0.18461595237190664 1104 16-17 0.19901409486065802 1104 18-19 0.07030090684342127 1104 20-21 0.05711647913713591 1104 22-23 0.023807211553751984 1104 24-25 0.14951795412550695 1104 26-27 0.16298338010292213 1104 28-29 0.13858527629273 1104 30-31 0.07703216528025081 1104 32-33 -0.0279772952067745 1104 34-35 0.12471029375615217 1104 36-37 0.04880789203331659 1104 38-39 -0.08154120302764767 1104 40-41 -0.045372324014330445 1104 42-43 0.016286641718991746 1104 44-45 0.41435184131564995 1104 46-47 0.2846937293199474 1104 48-49 0.5233128558457807 1104 50-51 0.3932367577876761 1104 52-53 0.5245328136428782 1104 54-55 0.5137360376422286 1104 56-57 0.483902915382334 1104 58-59 0.3276483167232982 1104 60-61 0.34607518329379516 1104 62-63 0.48670770322950574 1104 64-65 0.3458627577924709 1104 66-67 0.1122583848915113 1104 68-69 0.2638848586684901 1104 70-71 0.3580528945799095 1104 72-73 0.2260481918252637 1104 74-75 0.18050767183188654 1104 76-77 0.41395376187522004 1104 78-79 0.35572318020649973 1104 80-81 0.3749251976161325 1104 82-83 0.4494258960743309 1104 84-85 0.29111394475189556 1104 86-87 0.22544757834055673 1104 88-89 0.35536791583364646 1104 90-91 0.6044945857139545 1104 92-93 0.2849827306792072 1104 94-95 -0.00202636274640966 1104 96-97 0.30017830801087086 1104 98-99 0.17709784848124954 1104 100-101 0.7494154017884753 1105 1 -0.03950867493679411 1105 2 0.016172269493566205 1105 3 -0.14486526518466292 1105 4 -0.14259099126137187 1105 5 -0.27263950567574113 1105 6 -0.08557784702308169 1105 7 -0.08431369304681624 1105 8 -0.0910163546378584 1105 9 0.0031519226457916716 1105 10-11 -0.06423781217853275 1105 12-13 -0.029564408668136366 1105 14-15 -0.04860199707957236 1105 16-17 -0.3060372610323938 1105 18-19 -0.2629015733272908 1105 20-21 -0.2286546433181087 1105 22-23 -0.25343991338505845 1105 24-25 -0.41216315406833814 1105 26-27 -0.34554644488229513 1105 28-29 -0.12486770027237526 1105 30-31 -0.0810029605824738 1105 32-33 -0.03338677554489067 1105 34-35 0.009024443062259024 1105 36-37 -0.020759370730431215 1105 38-39 -0.10527807268094591 1105 40-41 -0.4728039148285461 1105 42-43 -0.47617897091750905 1105 44-45 -0.41303694409151603 1105 46-47 -0.21408885947479206 1105 48-49 -0.07072924602685049 1105 50-51 0.004653192944907403 1105 52-53 0.0018713768645568507 1105 54-55 0.12956439643630446 1105 56-57 -0.08683990676543729 1105 58-59 0.022824549974622244 1105 60-61 0.03505893631083268 1105 62-63 0.21801742154026016 1105 64-65 0.002631203850238961 1105 66-67 0.07572015563677326 1105 68-69 -0.029243178237397416 1105 70-71 -0.4057677422882584 1105 72-73 -0.24143877053776208 1105 74-75 -0.2520107694579501 1105 76-77 -0.24544764053162993 1105 78-79 -0.050296700997900956 1105 80-81 0.1034266057444988 1105 82-83 -0.04778009753028911 1105 84-85 -0.33510585041787877 1105 86-87 -0.292768559297901 1105 88-89 -0.27616970948342257 1105 90-91 -0.0824622677071929 1105 92-93 0.017773155830138165 1105 94-95 -0.034113465392504594 1105 96-97 -0.2947045386758447 1105 98-99 -0.07185850483712386 1105 100-101 0.3487657979503993 1106 1 -0.04323157657405474 1106 2 0.05675848453599741 1106 3 0.06105957717691268 1106 4 0.15084588976931457 1106 5 -0.014485010936411413 1106 6 0.0947364303841951 1106 7 0.036000311786821726 1106 8 0.05543519085468063 1106 9 0.18257352138105176 1106 10-11 0.1086772266177789 1106 12-13 0.1939164475765267 1106 14-15 0.29302931072874827 1106 16-17 0.2952090819379336 1106 18-19 0.19254626516374174 1106 20-21 0.22297262131454687 1106 22-23 0.20965134426972298 1106 24-25 0.14565312221230897 1106 26-27 0.23931595085829116 1106 28-29 0.10633039716361736 1106 30-31 0.22198876226465103 1106 32-33 0.008521421653050254 1106 34-35 0.20470392371861124 1106 36-37 0.3290741322042763 1106 38-39 0.31675110178435517 1106 40-41 0.3878564402440503 1106 42-43 0.42458264526246126 1106 44-45 0.17917131125546604 1106 46-47 0.4590135736992451 1106 48-49 0.41740698627857853 1106 50-51 0.5157423972115893 1106 52-53 0.44537253984542247 1106 54-55 0.5119454634712461 1106 56-57 0.6174710725007948 1106 58-59 0.7199918284176796 1106 60-61 0.5054642533139777 1106 62-63 0.6113275558282076 1106 64-65 0.6074755188726826 1106 66-67 0.7600980517316991 1106 68-69 0.7064591986047439 1106 70-71 0.4410140715984596 1106 72-73 0.8137914633304177 1106 74-75 0.6717117687477874 1106 76-77 0.8252818119587886 1106 78-79 0.5299751003504305 1106 80-81 0.6251632178358655 1106 82-83 0.6365080764080346 1106 84-85 0.5551571377160833 1106 86-87 0.44574927610396387 1106 88-89 0.3056216899096462 1106 90-91 -0.07332877930974391 1106 92-93 0.4109867536204703 1106 94-95 0.8478485196704604 1106 96-97 0.8564909238816227 1106 98-99 0.8566170362215537 1106 100-101 0.9109477953700349 1107 1 0.2771083385111197 1107 2 0.3398420182205868 1107 3 0.3121951851364031 1107 4 0.1418697273057674 1107 5 0.18076282782772068 1107 6 0.16746009911405224 1107 7 0.20964602256486842 1107 8 0.1824890043489802 1107 9 0.24955137191048493 1107 10-11 0.32101992956933856 1107 12-13 0.3085348423793306 1107 14-15 0.4248576651202782 1107 16-17 0.26125274146016153 1107 18-19 0.3480069025364898 1107 20-21 0.4680925762994548 1107 22-23 0.21086496133452926 1107 24-25 0.4091825866350405 1107 26-27 0.532587345814548 1107 28-29 0.5133211639547568 1107 30-31 0.5773387801683754 1107 32-33 0.5471576690314279 1107 34-35 0.5359273878344268 1107 36-37 0.6762958571006905 1107 38-39 0.592204449781697 1107 40-41 0.7262139009837298 1107 42-43 0.5753147798985054 1107 44-45 0.5089586405306221 1107 46-47 0.3125968771930232 1107 48-49 0.6640270179937531 1107 50-51 0.794846187686808 1107 52-53 0.9326282818271068 1107 54-55 0.7672737411325024 1107 56-57 0.681813877759371 1107 58-59 0.7346837563935367 1107 60-61 0.6393010224581808 1107 62-63 0.6441067261182454 1107 64-65 0.8196161654530698 1107 66-67 0.7472124821373463 1107 68-69 0.4469363329850218 1107 70-71 0.37908763034826976 1107 72-73 0.4642122009640559 1107 74-75 0.2241812045245517 1107 76-77 0.12903456146003833 1107 78-79 0.3717208418205793 1107 80-81 0.3442249345477144 1107 82-83 0.3723110586577647 1107 84-85 0.5670816607095865 1107 86-87 0.8503543709937276 1107 88-89 0.8439772323926391 1107 90-91 0.8321203485228814 1107 92-93 0.7111702668436557 1107 94-95 0.890180608268345 1107 96-97 0.8638205826877012 1107 98-99 1.2116140948946317 1107 100-101 1.415589337136879 1108 1 0.01870144079181557 1108 2 0.11761307778959917 1108 3 6.6131033321426E-5 1108 4 -0.0032484191135679907 1108 5 -0.09313128782758895 1108 6 0.009790860717075134 1108 7 0.005730381768678683 1108 8 0.03604669898220436 1108 9 -0.08337604724930969 1108 10-11 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-0.9674688914109879 2316 98-99 -1.3478014128151266 2316 100-101 -2.5851383250514637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 6.0 5 49.0 6 121.0 7 239.0 8 500.0 9 975.0 10 1920.0 11 3345.0 12 5228.0 13 7332.0 14 9135.0 15 13081.0 16 18272.0 17 25839.0 18 21136.0 19 9627.0 20 6599.0 21 6262.0 22 6455.0 23 6841.0 24 7748.0 25 8983.0 26 10232.0 27 12342.0 28 14872.0 29 18263.0 30 22172.0 31 27135.0 32 34248.0 33 43900.0 34 60139.0 35 83445.0 36 116008.0 37 151154.0 38 80418.0 39 3174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.621434340248406 19.521464447343646 15.938122750928303 17.918978461479647 2 16.64196032571757 22.4024597242844 39.608943018537516 21.34663693146051 3 19.22204504326949 28.896732541403136 31.849330203835425 20.031892211491947 4 12.34572030239 18.565045025244956 37.38439100952344 31.704843662841604 5 11.686220546744165 40.186508419993935 31.84774013526459 16.27953089799731 6 26.99161607124727 38.218006036810934 17.93639967653969 16.85397821540211 7 25.228083335523188 38.115777227261546 19.652075862942162 17.004063574273108 8 28.194319855422318 31.4900793958889 19.520734068564508 20.794866680124272 9 29.97884607804376 14.785767268635697 20.37393827259295 34.861448380727595 10-11 25.268604641440422 27.936554956599224 25.111652335113483 21.68318806684687 12-13 28.048326704577693 26.839143722850125 26.90735251342226 18.20517705914992 14-15 24.571037113972224 25.656247965602937 29.3687799709615 20.403934949463338 16-17 19.752280526566626 29.017841678840224 29.145350496955913 22.084527297637237 18-19 19.609815180012255 24.828026512405145 36.53285443141546 19.029303876167145 20-21 21.99663995633018 23.26194186156931 36.753896808555744 17.987521373544762 22-23 21.25874209404134 23.77730131332979 36.620818576539236 18.343138016089632 24-25 20.10637942073025 24.15174774723122 37.304286233026595 18.437586599011937 26-27 19.892080100812834 24.32856144625804 37.112799288098955 18.666559164830176 28-29 19.6845898001124 24.521572320921457 37.006111475754466 18.787726403211675 30-31 19.95474183495641 24.42609087227976 36.918192492328565 18.70097480043526 32-33 19.43579452815652 24.687199517436202 36.70136587055585 19.175640083851434 34-35 19.882178268675116 24.607231034033806 36.77724597675338 18.733344720537698 36-37 20.186315742951642 24.434799643087935 36.19014287232988 19.188741741630544 38-39 20.085221214993627 24.887963488909236 35.854490808018724 19.172324488078406 40-41 20.289313231950473 25.001508021780015 35.40476797666478 19.304410769604733 42-43 19.974390734797186 25.48820647947855 34.88127644986783 19.656126335856435 44-45 20.654749817843022 25.53781101064275 34.08742340448405 19.720015767030187 46-47 20.95582328837564 25.9971846446674 32.738210774198826 20.308781292758134 48-49 21.30307598220727 26.601775450432157 31.356456552587446 20.738692014773125 50-51 21.51614022577025 27.211341428381164 30.47725186444707 20.795266481401516 52-53 21.704924494665207 27.518103661941552 29.814110852641416 20.962860990751828 54-55 21.527131607017225 27.465936930017666 29.54639111224718 21.46054035071793 56-57 21.93269652278507 27.82530432050913 28.822864022991112 21.419135133714686 58-59 21.937886670437194 27.333460344686518 29.155675108143797 21.572977876732498 60-61 22.06618110365546 27.348127178090536 29.537042843619442 21.04864887463456 62-63 22.247226308280073 27.403244041273467 29.61986821492923 20.72966143551723 64-65 22.098037915829796 27.49957745881642 29.682338614650206 20.72004601070358 66-67 22.323359973817393 27.809762815681378 29.07039800668182 20.796479203819413 68-69 22.483729924814543 27.600569280927477 29.094125453223945 20.821575341034034 70-71 22.171570064913464 27.766645982430543 29.16907302570559 20.892710926950407 72-73 22.125901673807594 28.055060183255236 28.79998996647129 21.01904817646588 74-75 21.533326046797477 28.367081342227685 28.89523574033892 21.20435687063592 76-77 21.12323689838646 28.413341431790506 29.160776472429113 21.30264519739392 78-79 21.010303466102243 28.294875500779987 28.95834179413613 21.736479238981644 80-81 20.91093314476253 28.36045531726062 28.83616259214775 21.892448945829095 82-83 21.081157268523302 28.43941468868383 28.5085144389811 21.970913603811773 84-85 20.934925845230588 28.06766383808268 28.440946142231905 22.55646417445483 86-87 20.71326444439932 28.347645047199393 28.02125674724944 22.917833761151847 88-89 20.850257922678743 27.89494839456048 28.350770065082397 22.90402361767838 90-91 20.56207778618564 27.899393140897498 28.70286479781218 22.83566427510468 92-93 20.418431981958847 27.76078987382898 28.96803261359035 22.852745530621828 94-95 20.2531184416571 28.082995997357852 28.75249194633999 22.91139361464506 96-97 20.308793421835322 28.177519189100735 28.522600496420186 22.99108689264376 98-99 19.995879059350504 27.957357222844344 28.529272116461367 23.517491601343785 100-101 19.991029333244146 28.517821286639677 28.53567900541465 22.955470374701527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2592.0 1 1788.5 2 986.5 3 982.0 4 828.5 5 780.5 6 1213.0 7 2469.0 8 3974.5 9 4468.5 10 3686.5 11 2816.0 12 2691.0 13 2808.0 14 2952.5 15 3000.0 16 3132.0 17 3449.5 18 3673.0 19 3874.5 20 4241.5 21 4588.0 22 5057.0 23 5673.5 24 6186.5 25 7019.0 26 8531.5 27 10151.0 28 10971.5 29 11947.0 30 13458.0 31 14562.0 32 15316.0 33 16416.0 34 18125.5 35 19453.5 36 20785.5 37 21631.0 38 20562.5 39 19838.0 40 20371.0 41 21156.0 42 21873.0 43 23299.5 44 25149.5 45 25864.5 46 25736.5 47 26259.5 48 28855.5 49 28873.5 50 26326.5 51 24676.5 52 22943.5 53 21936.5 54 21216.0 55 20509.0 56 19942.5 57 18946.5 58 18331.5 59 17908.5 60 16616.5 61 14921.5 62 12450.0 63 8494.5 64 5311.5 65 3800.0 66 2691.0 67 1889.5 68 1399.5 69 1022.5 70 694.5 71 544.5 72 467.5 73 423.5 74 319.0 75 244.5 76 177.0 77 83.5 78 38.0 79 15.5 80 10.5 81 6.0 82 3.5 83 5.0 84 4.5 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022097546933397993 2 0.0035833859891996746 3 2.3889239927997832E-4 4 0.0 5 0.0027472625917197503 6 0.0 7 3.583385989199675E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0073459412778593336 14-15 0.0038820014882996476 16-17 0.0 18-19 4.7778479855995664E-4 20-21 6.569540980199404E-4 22-23 0.00143335439567987 24-25 5.972309981999458E-4 26-27 2.3889239927997832E-4 28-29 1.7916929945998374E-4 30-31 0.0 32-33 2.3889239927997832E-4 34-35 0.0 36-37 0.0019111391942398265 38-39 0.009734865270659115 40-41 8.958464972999186E-4 42-43 0.0 44-45 8.36123397479924E-4 46-47 2.986154990999729E-4 48-49 1.1944619963998916E-4 50-51 0.0022097546933397996 52-53 5.375078983799512E-4 54-55 0.0 56-57 4.7778479855995664E-4 58-59 1.7916929945998374E-4 60-61 0.0029264318911797343 62-63 5.972309981999458E-5 64-65 5.972309981999458E-5 66-67 0.0 68-69 5.972309981999458E-5 70-71 6.569540980199404E-4 72-73 4.7778479855995664E-4 74-75 0.004359786286859604 76-77 5.972309981999458E-5 78-79 3.583385989199675E-4 80-81 0.002687539491899756 82-83 1.7916929945998374E-4 84-85 0.0034639397895596855 86-87 4.7778479855995664E-4 88-89 0.0032847704900997015 90-91 0.00209030849369981 92-93 0.0 94-95 0.0 96-97 3.583385989199675E-4 98-99 0.001134738896579897 100-101 0.00286670879135974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 837197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.20547830312512 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28939601002642 35.32950389210473 2 14.079780407032615 13.574450978479538 3 5.332574913646341 7.7117797289870404 4 2.4445483791657208 4.713624954112514 5 1.3319553384865306 3.2103772085072064 6 0.8299745622267787 2.4005592450941253 7 0.5189003877776375 1.750968896914868 8 0.36275077451873516 1.3989259672403778 9 0.2745870291877403 1.1912939170046277 >10 1.3536768064604436 11.89558296128511 >50 0.10701820456347184 3.6143235708041668 >100 0.06860075466673186 6.382138623062937 >500 0.003991316634121963 1.36558261627167 >1k 0.0014967437377957362 1.4135631960438078 >5k 2.494572896326227E-4 0.9385674815076069 >10k+ 4.989145792652454E-4 3.1087567625797123 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14321 1.7105890250442846 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11531 1.3773341280487148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7805 0.9322775881901153 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2911 0.3477078871520084 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2534 0.3026766698877325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2393 0.28583475573849404 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1775 0.21201700436098073 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1078 0.1287630032119083 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1064 0.12709075641694845 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 978 0.11681838324790939 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 0.11251832006086979 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 933 0.11144330426410987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 911 0.10881548787203012 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1944619963998916E-4 0.0 18-19 0.0 0.0 0.0 1.1944619963998916E-4 0.0 20-21 0.0 0.0 0.0 2.3889239927997832E-4 0.0 22-23 0.0 0.0 0.0 8.361233974799241E-4 0.0 24-25 0.0 0.0 0.0 0.00209030849369981 0.0 26-27 0.0 0.0 0.0 0.003702832188839664 0.0 28-29 0.0 0.0 0.0 0.007704279876779301 0.0 30-31 1.1944619963998916E-4 0.0 0.0 0.017021083448698456 0.0 32-33 1.1944619963998916E-4 0.0 0.0 0.026158717721157625 0.0 34-35 1.1944619963998916E-4 0.0 0.0 0.033683828298476944 0.0 36-37 1.1944619963998916E-4 0.0 0.0 0.03834223008443652 0.0 38-39 1.1944619963998916E-4 0.0 0.0 0.04365758596841604 0.0 40-41 1.1944619963998916E-4 0.0 0.0 0.05082435794681539 0.0 42-43 1.1944619963998916E-4 0.0 0.0 0.05900642262215464 0.0 44-45 1.1944619963998916E-4 0.0 0.0 0.06617319460055399 0.0 46-47 1.1944619963998916E-4 0.0 0.0 0.07447470547553324 0.0 48-49 1.1944619963998916E-4 0.0 0.0 0.08779295673539203 0.0 50-51 1.7916929945998374E-4 0.0 0.0 0.10750157967599024 0.0 52-53 2.3889239927997832E-4 0.0 0.0 0.15450365923432596 0.0 54-55 2.3889239927997832E-4 0.0 0.0 0.20401410898510147 0.0 56-57 2.3889239927997832E-4 0.0 0.0 0.26403582430419603 0.0 58-59 2.3889239927997832E-4 0.0 0.0 0.3338521279937697 0.0 60-61 2.3889239927997832E-4 0.0 0.0 0.4340674894917206 0.0 62-63 2.3889239927997832E-4 0.0 0.0 0.4980906524987547 0.0 64-65 2.3889239927997832E-4 0.0 0.0 0.5557234438250496 0.0 66-67 2.3889239927997832E-4 0.0 0.0 0.6946393740063569 0.0 68-69 2.3889239927997832E-4 0.0 0.0 0.8733906117676007 0.0 70-71 2.3889239927997832E-4 0.0 0.0 0.9831019461369308 0.0 72-73 2.3889239927997832E-4 0.0 0.0 1.1041606694720598 0.0 74-75 2.3889239927997832E-4 0.0 0.0 1.3739896344587952 0.0 76-77 2.3889239927997832E-4 0.0 0.0 1.5286724629925812 0.0 78-79 2.3889239927997832E-4 0.0 0.0 1.641728290951831 0.0 80-81 2.3889239927997832E-4 0.0 0.0 1.8084751856492558 0.0 82-83 2.3889239927997832E-4 0.0 0.0 2.1140185643283482 0.0 84-85 2.3889239927997832E-4 0.0 0.0 2.42248837489862 0.0 86-87 2.3889239927997832E-4 0.0 0.0 2.8590045114829605 0.0 88-89 2.3889239927997832E-4 0.0 0.0 3.4322865466550883 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6990 0.0 49.00288 1 GTATCAA 12140 0.0 40.18975 1 TATCAAC 16105 0.0 30.321035 2 ATCAACG 16125 0.0 30.253971 3 TCAACGC 16280 0.0 29.907568 4 GTGTAAG 175 1.5097612E-10 29.86195 1 CAACGCA 16540 0.0 29.408718 5 AACGCAG 16950 0.0 28.727097 6 ACGCAGA 18990 0.0 25.567568 7 CGCAGAG 18980 0.0 25.508997 8 GCAGAGT 19355 0.0 24.548346 9 GAGTACT 11640 0.0 24.530025 12-13 AGTACTT 11685 0.0 23.317457 12-13 GTACATG 10245 0.0 23.046658 1 GTACTTT 12400 0.0 22.983454 14-15 TACATGG 10155 0.0 22.967472 2 GATCGTC 105 0.0021936728 22.62269 7 GTACACG 130 2.937385E-4 21.926607 1 ACATGGG 10225 0.0 21.64882 3 CATGGGT 1205 0.0 21.28719 4 >>END_MODULE