FastQCFastQC Report
Thu 26 May 2016
SRR936991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815039
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72170.8854790997731397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50050.6140810439745829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33710.41359984000765604No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.1371713500826341No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10490.12870549752833912No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8710.10686605180856376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74800.032.3455051
GGTATCA48150.030.4055021
TATCAAC91950.026.1575182
ATCAACG91850.026.080893
GTACATG59200.026.0148351
TCAACGC92050.025.972594
CAACGCA93000.025.6034895
GTATTAC1309.96211E-625.5948431
AACGCAG94750.025.1290596
TACATGG61450.024.8302382
ACATGGG60650.024.372533
ATACACG1000.00164827523.7622576
GAGTACT47800.023.40708412-13
ACGCAGA103950.022.6736537
CGCAGAG103650.022.6003558
CATGGGA42750.022.458794
AGTACTT49850.021.87267112-13
GCAGAGT107550.021.5157369
GTACTTT51800.021.41680714-15
ATGGGAG16250.021.3507865
Produced by FastQC (version 0.11.5)