FastQCFastQC Report
Thu 26 May 2016
SRR936991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815039
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76530.9389734724350615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58000.7116223886218942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41060.5037795737381892No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14050.17238438896788988No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13360.16391853641359494No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12430.1525080394925887No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.12490199855467038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.12244812824907765No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9520.11680422654621436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT550.002579889534.5435376
GTATCAA88500.031.2451381
TATTCCG953.4909346E-529.9983335
CCGTTGC953.4909346E-529.9983339
GGTATCA59100.028.7804951
TATCAAC107800.025.687352
ATCAACG107950.025.6076563
TCAACGC108450.025.4895944
CAACGCA110050.025.1190035
AACGCAG111350.024.8683996
CCTATAC1401.779422E-523.748683
GAGTACT58500.023.70808612-13
GTACATG57000.023.5059051
AGTACTT59900.023.15397312-13
ACGCAGA122650.022.4997717
TACATGG59250.022.3658472
CGCAGAG123100.022.3017678
GTACTTT62250.022.08981114-15
ATTCCGT1302.9415966E-421.921866
TCCGTTG1302.9415966E-421.921868
Produced by FastQC (version 0.11.5)