##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936991_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 815039 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.122443220508465 33.0 31.0 34.0 31.0 34.0 2 32.10345271821348 33.0 31.0 34.0 30.0 34.0 3 32.27502855691568 33.0 31.0 34.0 30.0 34.0 4 36.03645592419504 37.0 35.0 37.0 35.0 37.0 5 35.973934989613994 37.0 35.0 37.0 35.0 37.0 6 36.03173467772708 37.0 35.0 37.0 35.0 37.0 7 35.973014788249394 37.0 35.0 37.0 35.0 37.0 8 35.95552728151659 37.0 35.0 37.0 35.0 37.0 9 37.629317124702006 39.0 37.0 39.0 35.0 39.0 10-11 37.55625559022329 39.0 37.0 39.0 35.0 39.0 12-13 37.45405189199535 39.0 37.0 39.0 35.0 39.0 14-15 38.60096142638574 40.0 38.0 41.0 34.0 41.0 16-17 38.54560579309702 40.0 38.0 41.0 34.0 41.0 18-19 38.65894638170688 40.0 38.0 41.0 34.5 41.0 20-21 38.74479810168593 40.0 38.0 41.0 34.0 41.0 22-23 38.66879008243777 40.0 38.0 41.0 34.0 41.0 24-25 38.54891422373653 40.0 38.0 41.0 34.0 41.0 26-27 38.36384761956177 40.0 38.0 41.0 34.0 41.0 28-29 38.178963215257184 40.0 38.0 41.0 34.0 41.0 30-31 37.98183767893316 40.0 37.0 41.0 33.5 41.0 32-33 37.8999943560983 40.0 37.0 41.0 33.0 41.0 34-35 37.76882701318587 40.0 37.0 41.0 33.0 41.0 36-37 37.5612510566979 40.0 36.0 41.0 33.0 41.0 38-39 37.38226207580251 40.0 36.0 41.0 33.0 41.0 40-41 37.16512338673364 40.0 36.0 41.0 32.0 41.0 42-43 36.87831747928627 39.5 36.0 41.0 31.0 41.0 44-45 36.38137377475188 39.0 35.0 41.0 30.0 41.0 46-47 35.95210413244028 39.0 35.0 41.0 29.5 41.0 48-49 35.49168935474253 39.0 35.0 41.0 27.0 41.0 50-51 35.17629524476743 39.0 35.0 40.0 26.0 41.0 52-53 34.91721009669476 39.0 35.0 40.0 25.5 41.0 54-55 34.67433276199053 38.0 34.5 40.0 24.0 41.0 56-57 34.397208599833874 38.0 34.0 40.0 23.5 41.0 58-59 34.06744094454376 38.0 34.0 40.0 23.0 41.0 60-61 33.72694190093971 37.5 33.5 40.0 22.0 41.0 62-63 33.352025485896995 37.0 33.0 40.0 21.5 41.0 64-65 32.95506534043156 36.0 32.5 39.0 19.5 40.5 66-67 32.577962526946564 36.0 32.0 39.0 18.5 40.0 68-69 32.13247709618804 35.0 31.5 38.5 17.0 40.0 70-71 31.65393005733468 35.0 31.0 38.0 13.0 40.0 72-73 31.320449696272203 35.0 31.0 37.0 9.5 39.5 74-75 30.873976582715674 35.0 31.0 37.0 7.5 39.0 76-77 29.4960179819616 33.5 28.5 35.0 7.0 37.5 78-79 30.202216703740557 34.0 30.0 36.0 3.5 38.0 80-81 30.036628922051584 34.0 30.0 35.5 2.0 37.0 82-83 29.71188188540671 34.0 30.0 35.0 2.0 37.0 84-85 29.349056302827226 34.0 30.0 35.0 2.0 36.5 86-87 28.99369355331463 34.0 29.0 35.0 2.0 36.0 88-89 28.625155360663722 34.0 29.0 35.0 2.0 36.0 90-91 28.229822131210902 34.0 29.0 35.0 2.0 35.0 92-93 27.79724405825979 33.5 27.5 35.0 2.0 35.0 94-95 27.333414720031804 33.0 27.0 35.0 2.0 35.0 96-97 26.70388533554836 33.0 25.5 35.0 2.0 35.0 98-99 26.045135876933493 33.0 24.5 35.0 2.0 35.0 100-101 23.254522789706996 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26326762680599103 1101 2 0.1450546966391073 1101 3 0.1988835103321449 1101 4 0.18633957943210788 1101 5 0.17851775121137337 1101 6 0.10806118351604255 1101 7 0.23989748227986496 1101 8 0.23863562615595413 1101 9 0.21961545753291034 1101 10-11 0.19669967797671006 1101 12-13 0.32021081469866175 1101 14-15 0.45647836282748244 1101 16-17 0.4245798188115444 1101 18-19 0.42946372181538095 1101 20-21 0.483539393762463 1101 22-23 0.30445098754437083 1101 24-25 0.29096307820179845 1101 26-27 0.41010931593493183 1101 28-29 0.34517281512017206 1101 30-31 0.3230373555057042 1101 32-33 0.46233628757831724 1101 34-35 0.4994687933164883 1101 36-37 0.44018665044138316 1101 38-39 0.398884230207166 1101 40-41 0.3754271783779686 1101 42-43 0.550768584085148 1101 44-45 0.6811687451429407 1101 46-47 0.6657071519796887 1101 48-49 0.7805056331490619 1101 50-51 0.7134536798715132 1101 52-53 0.5840837926670801 1101 54-55 0.6870292561209439 1101 56-57 0.7539235819810983 1101 58-59 0.8307606245099706 1101 60-61 0.7463902259788426 1101 62-63 0.8273996642896506 1101 64-65 0.883496979520423 1101 66-67 0.9136181657587699 1101 68-69 0.763816834436664 1101 70-71 0.9181550047415215 1101 72-73 1.0551795412732368 1101 74-75 0.9670041218887597 1101 76-77 0.7719775088371321 1101 78-79 0.899299135538147 1101 80-81 1.147069461163138 1101 82-83 1.1597625640777807 1101 84-85 1.0549875252070215 1101 86-87 0.8325613421151736 1101 88-89 1.049723637902165 1101 90-91 1.063119953212471 1101 92-93 1.2169623048231522 1101 94-95 1.3678847170736326 1101 96-97 1.3067828830137103 1101 98-99 1.3991560755384356 1101 100-101 1.3558656520485393 1102 1 0.3221654215528389 1102 2 0.16291726167148113 1102 3 0.21095363560981895 1102 4 0.23058847273760819 1102 5 0.26766059507119877 1102 6 0.26136688399154906 1102 7 0.29457044261319965 1102 8 0.2791231383415749 1102 9 0.3577506900077623 1102 10-11 0.34346981531288634 1102 12-13 0.3385419179354301 1102 14-15 0.4548628140421229 1102 16-17 0.48804241057602127 1102 18-19 0.49050734889674175 1102 20-21 0.40893213047595367 1102 22-23 0.4148850791105474 1102 24-25 0.5176353237419633 1102 26-27 0.533414043013245 1102 28-29 0.5239059749854036 1102 30-31 0.581113283392952 1102 32-33 0.6449724796484801 1102 34-35 0.6893132095760066 1102 36-37 0.7275516642214868 1102 38-39 0.7448920667810341 1102 40-41 0.6795723976175267 1102 42-43 0.7670981211694041 1102 44-45 0.7032808427267341 1102 46-47 0.7452678447479357 1102 48-49 0.963962675096667 1102 50-51 0.7488068850360392 1102 52-53 0.8013104532899646 1102 54-55 0.7326358205954406 1102 56-57 0.8208686111902779 1102 58-59 0.7413649879445217 1102 60-61 0.7723610444024871 1102 62-63 0.900918030260847 1102 64-65 0.6940760636844132 1102 66-67 0.849145129587626 1102 68-69 0.6805884681894305 1102 70-71 0.7178756252168554 1102 72-73 0.9022857185680913 1102 74-75 0.8547554436023468 1102 76-77 0.7553273318095961 1102 78-79 0.8723418444299149 1102 80-81 0.7381750805706453 1102 82-83 0.8596060941099886 1102 84-85 0.7403034652399434 1102 86-87 0.7087415862199471 1102 88-89 0.9824052309611169 1102 90-91 1.131546870695992 1102 92-93 0.9834116278762508 1102 94-95 1.116114804926358 1102 96-97 1.2983884217890598 1102 98-99 1.4154430446555928 1102 100-101 1.421937529441177 1103 1 0.1681877237740963 1103 2 -0.061637828883121415 1103 3 0.07910096594344651 1103 4 0.08601564189886801 1103 5 0.20652319332034352 1103 6 0.1963238936842231 1103 7 0.243277745225015 1103 8 0.324728355159408 1103 9 0.3161312704778254 1103 10-11 0.30448208095791784 1103 12-13 0.40613848164658606 1103 14-15 0.4378297297752525 1103 16-17 0.48469775889947897 1103 18-19 0.4810314474266164 1103 20-21 0.41677712107370724 1103 22-23 0.4493266035377701 1103 24-25 0.49379367150500997 1103 26-27 0.38193959159116275 1103 28-29 0.2981852366247182 1103 30-31 0.40872951659171264 1103 32-33 0.49620494185207775 1103 34-35 0.47342538017630176 1103 36-37 0.5251533183037722 1103 38-39 0.5816232621585016 1103 40-41 0.6228952304221664 1103 42-43 0.7234637849123828 1103 44-45 0.7646061845443697 1103 46-47 0.8292885878792049 1103 48-49 0.6896687347352639 1103 50-51 0.6071113458472581 1103 52-53 0.8085554511222384 1103 54-55 0.7434139344111017 1103 56-57 0.5712414769434062 1103 58-59 0.7325006125215268 1103 60-61 0.7940312942500682 1103 62-63 0.7841092158979208 1103 64-65 0.7632327658578575 1103 66-67 0.8815038481981006 1103 68-69 1.0341983591317678 1103 70-71 0.8234452566184203 1103 72-73 0.9446508696042777 1103 74-75 0.6688630585564006 1103 76-77 0.8433763356748187 1103 78-79 0.8205702537334965 1103 80-81 0.7113940142348767 1103 82-83 0.7629364864565034 1103 84-85 0.7789548995870028 1103 86-87 0.6870582303705 1103 88-89 0.8024999296882136 1103 90-91 1.0923198207469227 1103 92-93 0.9530080626243524 1103 94-95 1.0092261854968712 1103 96-97 1.2115483155360813 1103 98-99 1.1975264061668298 1103 100-101 1.549937558798618 1104 1 0.2600727862617518 1104 2 0.060698262885082954 1104 3 0.1080580761332186 1104 4 0.13326777334825124 1104 5 0.1850666673988428 1104 6 0.1651401080648469 1104 7 0.17826514956319528 1104 8 0.26649520823239925 1104 9 0.19506701129771642 1104 10-11 0.19342850000673906 1104 12-13 0.2767253194059407 1104 14-15 0.2759254413107044 1104 16-17 0.32970074802885563 1104 18-19 0.30577711318901635 1104 20-21 0.2717349440062051 1104 22-23 0.14723376105003894 1104 24-25 0.1639122553678405 1104 26-27 0.2778794758737675 1104 28-29 0.3456439601696317 1104 30-31 0.44155926017219116 1104 32-33 0.42644427879474733 1104 34-35 0.3506254245936091 1104 36-37 0.4939827674905075 1104 38-39 0.39333038429187184 1104 40-41 0.5379084071242559 1104 42-43 0.5727483952215877 1104 44-45 0.5702620950796629 1104 46-47 0.4585735984605961 1104 48-49 0.521942422778622 1104 50-51 0.39302286245274587 1104 52-53 0.4661642380005375 1104 54-55 0.40328006273320227 1104 56-57 0.2710157105190092 1104 58-59 0.1242941288868522 1104 60-61 0.3381251490390653 1104 62-63 0.5348755434522801 1104 64-65 0.6091328166020347 1104 66-67 0.6097012805165889 1104 68-69 0.6592527509569202 1104 70-71 0.8328106573489791 1104 72-73 0.6696525804450708 1104 74-75 0.8027450660634408 1104 76-77 0.6383917848899614 1104 78-79 0.5529979820080761 1104 80-81 0.8065338706275469 1104 82-83 0.8078392784195536 1104 84-85 0.857668101983986 1104 86-87 0.8457006233267457 1104 88-89 0.8799393626962058 1104 90-91 0.9740648912658401 1104 92-93 1.1941714085382849 1104 94-95 1.112617136504845 1104 96-97 1.0928195154358846 1104 98-99 1.084803625643815 1104 100-101 1.1971040836484192 1105 1 0.23320606749651773 1105 2 0.1330960913657364 1105 3 0.2089175426502976 1105 4 0.1721938716930822 1105 5 0.09094485583903378 1105 6 0.11744275278618943 1105 7 0.22896776104373373 1105 8 0.18394455196627035 1105 9 0.21866957914970442 1105 10-11 0.22093925931935843 1105 12-13 0.33489991991701373 1105 14-15 0.39843617665159314 1105 16-17 0.3868421668677584 1105 18-19 0.41068900109080175 1105 20-21 0.4261844171874287 1105 22-23 0.39168510402640777 1105 24-25 0.41605452919854 1105 26-27 0.4028636944831874 1105 28-29 0.4184301307173186 1105 30-31 0.45962315922242425 1105 32-33 0.4966890461650664 1105 34-35 0.5940411887245389 1105 36-37 0.49259120841696813 1105 38-39 0.4603373696547308 1105 40-41 0.28194543865667043 1105 42-43 0.32728744277670785 1105 44-45 0.4328804954619301 1105 46-47 0.5247751336103477 1105 48-49 0.6331744579627241 1105 50-51 0.6433592657659588 1105 52-53 0.6195677445855736 1105 54-55 0.6032514564657134 1105 56-57 0.776996258257121 1105 58-59 0.7437436739832748 1105 60-61 0.6879030908900319 1105 62-63 0.7818990533294325 1105 64-65 0.8903488067065055 1105 66-67 0.8112895676084477 1105 68-69 0.755419750830665 1105 70-71 0.42089998241685933 1105 72-73 0.48983382272417586 1105 74-75 0.5197899267637034 1105 76-77 0.6638481667245024 1105 78-79 0.6754695635191617 1105 80-81 0.9394095835868264 1105 82-83 0.9921866959448096 1105 84-85 0.9210106085003034 1105 86-87 0.999297501887547 1105 88-89 0.9804148819638847 1105 90-91 1.355624156580184 1105 92-93 1.3709213849660777 1105 94-95 1.275847854841782 1105 96-97 1.375603256848013 1105 98-99 1.376967505996106 1105 100-101 1.3208054150384463 1106 1 0.15521307362006098 1106 2 0.21754556820262394 1106 3 0.0779136521134447 1106 4 0.19701093322556318 1106 5 0.12551337363203174 1106 6 0.1089426975351131 1106 7 0.20031182600178 1106 8 0.24409073052913755 1106 9 0.33637589886831165 1106 10-11 0.326058379205584 1106 12-13 0.3267177377434578 1106 14-15 0.4275972643946062 1106 16-17 0.49087492142812295 1106 18-19 0.3729632789161741 1106 20-21 0.4145874314565603 1106 22-23 0.5606106069188286 1106 24-25 0.3410551391130241 1106 26-27 0.3234858359575128 1106 28-29 0.3259369581718019 1106 30-31 0.1641109817416222 1106 32-33 0.4441115475606878 1106 34-35 0.4146179812632127 1106 36-37 0.3057959287245353 1106 38-39 0.49358057915389963 1106 40-41 0.37369092780091506 1106 42-43 0.2310601585034604 1106 44-45 0.20092140709034823 1106 46-47 0.0381537871774853 1106 48-49 0.19182885745527756 1106 50-51 0.31443095009144173 1106 52-53 0.45395443610804875 1106 54-55 0.32991218184776727 1106 56-57 0.4970297246235589 1106 58-59 0.41983531327554147 1106 60-61 0.5754391655378299 1106 62-63 0.8442732136206956 1106 64-65 0.6909648920887932 1106 66-67 0.7031998054176967 1106 68-69 0.6425604573843096 1106 70-71 0.44553335205804245 1106 72-73 0.7450708354921964 1106 74-75 1.01030970306142 1106 76-77 0.6586574357025299 1106 78-79 0.6194944824722874 1106 80-81 0.4590509825727871 1106 82-83 0.6696761157223996 1106 84-85 0.5118318978164673 1106 86-87 0.5252310656967971 1106 88-89 0.31391970889885457 1106 90-91 0.7129730520609314 1106 92-93 0.9482901134307156 1106 94-95 0.7299320574826389 1106 96-97 0.9706250630846363 1106 98-99 1.0393023447418912 1106 100-101 1.326871552729866 1107 1 0.057800841339208375 1107 2 0.03875334033774891 1107 3 0.025235356683992904 1107 4 0.05299238608491663 1107 5 0.03251933521295314 1107 6 0.13730878276363967 1107 7 0.18490886729124867 1107 8 0.1628807504010794 1107 9 0.38365565157310044 1107 10-11 0.3295988958759253 1107 12-13 0.32613151561935894 1107 14-15 0.24318017452929297 1107 16-17 0.21333241756413912 1107 18-19 0.20358262093428436 1107 20-21 -0.07002064979757705 1107 22-23 0.07732842542181118 1107 24-25 0.19172636896508521 1107 26-27 0.38244918327480093 1107 28-29 0.057433977381336376 1107 30-31 0.35629247876082815 1107 32-33 0.5124941927362272 1107 34-35 0.4198078686555675 1107 36-37 0.42833732859205753 1107 38-39 0.31006876639170144 1107 40-41 0.2532526412034244 1107 42-43 0.27489985821642193 1107 44-45 0.2309677749419663 1107 46-47 0.308332634605172 1107 48-49 0.3584914761349012 1107 50-51 0.17686967828750966 1107 52-53 0.27694737053065666 1107 54-55 0.1772559663476585 1107 56-57 0.2762050392625568 1107 58-59 0.12642838017772817 1107 60-61 0.23056060514922194 1107 62-63 0.1998583317483167 1107 64-65 0.3471792882027316 1107 66-67 0.49298011012071896 1107 68-69 0.443323318943154 1107 70-71 0.4802805368670491 1107 72-73 0.5877678988615891 1107 74-75 0.6983732932579336 1107 76-77 0.6929873098469344 1107 78-79 0.6291941954332074 1107 80-81 0.7143910134846898 1107 82-83 0.6431835124987302 1107 84-85 0.7591569143764119 1107 86-87 0.983781518335352 1107 88-89 0.9931579513364817 1107 90-91 1.2272576623501763 1107 92-93 1.3771662450270945 1107 94-95 1.3550181692070815 1107 96-97 1.323060479291307 1107 98-99 1.4431254845078456 1107 100-101 1.4167942729771639 1108 1 0.029621569630521094 1108 2 -0.13701696778549888 1108 3 -0.11022682818995833 1108 4 0.0728243188580251 1108 5 0.023023536893624907 1108 6 0.019633712735625863 1108 7 -0.027752197134525147 1108 8 0.011340134154579573 1108 9 -0.0107140963260548 1108 10-11 0.07846444209442183 1108 12-13 0.01062731393869143 1108 14-15 -0.07075539970039557 1108 16-17 -0.23156954322016787 1108 18-19 0.046369735780217525 1108 20-21 0.06743033059456849 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-0.7028099888188812 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 22.0 6 59.0 7 118.0 8 246.0 9 538.0 10 951.0 11 1585.0 12 2524.0 13 3460.0 14 4374.0 15 6388.0 16 8959.0 17 12797.0 18 10914.0 19 5871.0 20 4779.0 21 4706.0 22 4804.0 23 5067.0 24 5740.0 25 6789.0 26 8067.0 27 9850.0 28 12497.0 29 15150.0 30 18974.0 31 23741.0 32 30070.0 33 39568.0 34 54481.0 35 78515.0 36 117587.0 37 175057.0 38 130358.0 39 10420.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24940358731187 19.81268223721026 15.502066582236228 20.435847593241636 2 15.56241012578709 22.044641411092623 39.89433491855495 22.49861354456534 3 19.24274622587801 27.37367243173207 30.69467892970023 22.68890241268969 4 12.70933047375647 17.796449004280777 37.83598085490388 31.65823966705888 5 12.871308995340003 39.11121417581366 33.20646559233984 14.811011236506499 6 29.69072645603462 37.77659719350853 17.4908194577192 15.041856892737648 7 27.033095389417426 35.137502791280895 20.689832866688423 17.139568952613253 8 27.057355537587775 33.73850821862512 19.685683752556628 19.51845249123048 9 27.862102304306912 16.52104991294895 20.503926805956525 35.11292097678761 10-11 24.66559841381629 27.72551709245458 26.649064215410817 20.95982027831831 12-13 26.697475197353732 25.558197572125824 27.63909235688327 20.105234873637173 14-15 23.41072022320404 25.756374848320142 27.7033982422927 23.129506686183117 16-17 20.953291805668194 29.31853567743384 27.91467647560423 21.813496041293728 18-19 20.9170972186607 26.85466585034581 32.06245345314764 20.165783477845846 20-21 22.332827409691404 25.6230897497089 32.03165356050409 20.012429280095606 22-23 21.716077390161697 25.96231591371701 31.968715607473996 20.352891088647294 24-25 21.653728317990133 25.979762434986174 32.248327953090666 20.118181293933024 26-27 21.464959400068217 26.105363509875758 32.20406465560608 20.225612434449943 28-29 20.924368819944515 26.325149904112898 32.30787918348116 20.44260209246142 30-31 21.090402866936664 26.205769335778484 32.22076541220804 20.48306238507681 32-33 21.092329437400466 26.33484573725397 31.973907965027397 20.59891686031817 34-35 21.431914874037993 26.13114281419952 31.97155924186607 20.465383069896415 36-37 21.782285122184796 25.995395179600877 31.712771106807168 20.509548591407157 38-39 21.511815873471598 26.211356317862055 31.795469227074214 20.481358581592133 40-41 21.35912919340699 26.244072958211174 31.795569281282727 20.601228567099113 42-43 21.216166474442343 26.62443866408186 31.25782189394125 20.90157296753454 44-45 21.574099351256844 26.840973574835512 30.764289986657054 20.82063708725059 46-47 21.99471436336175 27.024903102796312 29.994822333655197 20.98556020018674 48-49 22.065263134647545 27.295196916957348 29.374974694462473 21.264565253932634 50-51 22.12317447385953 27.576165066947716 29.134250017483826 21.166410441708923 52-53 22.17798385088138 27.78612785557324 28.98412203737536 21.051766256170026 54-55 21.63730707268263 27.654357191147582 29.098083579445134 21.61025215672465 56-57 22.023511916994824 28.08387630692481 28.56048248323535 21.332129292845018 58-59 22.255445368407813 27.409688630138245 28.846572661658655 21.488293339795284 60-61 22.126745105545258 27.28790798369875 29.39102204276042 21.194324867995576 62-63 22.040663084833977 27.352556135350582 29.540304206301784 21.06647657351366 64-65 21.94610677422818 27.516157232029204 29.27865282272288 21.25908317101974 66-67 21.861469205768067 27.695055083253685 28.914505931617995 21.528969779360253 68-69 21.939256894455358 27.669228098287324 29.004194891287412 21.38732011596991 70-71 22.022517954673308 27.728751463887903 28.800173243349853 21.448557338088936 72-73 21.9923218398138 27.66076224573303 28.52403381924055 21.822882095212623 74-75 21.954981129667893 28.209306092000848 28.325804013565044 21.50990876476622 76-77 21.92832963411477 28.027316551273135 28.4509782991447 21.593375515467397 78-79 21.919420885542078 27.79074582457862 28.445132892658314 21.844700397220986 80-81 21.84021580884451 28.209877414244882 28.266197780192286 21.683708996718327 82-83 21.94574757660362 28.15010855515209 28.20630325383256 21.69784061441173 84-85 21.836769625577375 28.027064980861997 28.035469873368328 22.100695520192293 86-87 22.003475973392177 27.838522447512247 28.04495821286135 22.113043366234226 88-89 22.07763924095673 27.68292054486605 27.996346128835153 22.243094085342065 90-91 21.82115658552925 27.792296546046373 28.251784888293415 22.134761980130964 92-93 21.794369223048975 27.923282151702033 28.05904260964359 22.2233060156054 94-95 21.69748121864406 27.96676715290226 27.881617644352342 22.454133984101336 96-97 21.80597024997408 27.88959104910343 27.80634201793934 22.49809668298315 98-99 21.691600951645935 27.988463931073156 27.53116836746588 22.788766749815032 100-101 21.54028024614939 28.13197248947436 27.56279343119025 22.764953833186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 823.0 1 585.5 2 372.5 3 425.5 4 380.5 5 353.5 6 603.5 7 1344.5 8 2280.0 9 2675.0 10 2336.5 11 1901.0 12 1822.5 13 1874.0 14 2014.0 15 2117.5 16 2213.5 17 2333.0 18 2402.5 19 2509.0 20 2624.0 21 2827.0 22 3230.5 23 3645.5 24 4075.5 25 4879.5 26 5939.0 27 7042.0 28 8009.5 29 9074.5 30 10518.0 31 12091.0 32 13705.5 33 15221.0 34 16964.0 35 18399.5 36 20142.0 37 22314.5 38 23287.5 39 23314.0 40 23468.5 41 24227.0 42 24869.0 43 25643.0 44 27303.0 45 28637.5 46 28683.5 47 29323.5 48 33813.0 49 33562.5 50 28536.0 51 26428.0 52 25233.5 53 24819.0 54 24002.5 55 22458.0 56 20182.0 57 17968.0 58 16684.0 59 15288.0 60 13281.5 61 10876.0 62 8578.5 63 6561.5 64 4786.5 65 3490.0 66 2484.5 67 1866.5 68 1290.5 69 745.0 70 498.5 71 336.5 72 250.5 73 195.0 74 121.0 75 102.5 76 85.5 77 42.0 78 21.0 79 13.5 80 8.0 81 6.0 82 4.5 83 2.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020489817051699367 2 0.001349628668076006 3 0.0018404027291945538 4 0.0 5 0.0020857897597538278 6 0.0 7 1.2269351527963694E-4 8 0.0 9 0.0 10-11 1.2269351527963694E-4 12-13 6.134675763981846E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.003435418427829834 22-23 0.0 24-25 0.0024538703055927385 26-27 0.0015950156986352798 28-29 0.0022084832750334646 30-31 0.011349150163366416 32-33 1.2269351527963694E-4 34-35 0.0014109754257158246 36-37 0.0028832976090714677 38-39 0.0015336689409954616 40-41 0.0030059911243511045 42-43 0.0023311767903131018 44-45 1.840402729194554E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0012269351527963693 54-55 0.0036808054583891076 56-57 6.74814334038003E-4 58-59 0.0014109754257158246 60-61 0.0011655883951565509 62-63 0.0 64-65 1.840402729194554E-4 66-67 0.0 68-69 0.0 70-71 6.134675763981847E-5 72-73 0.0 74-75 3.680805458389108E-4 76-77 2.453870305592739E-4 78-79 1.840402729194554E-4 80-81 0.00717757064385876 82-83 0.0019017494868343722 84-85 0.004601006822986384 86-87 0.0019017494868343722 88-89 0.0015950156986352798 90-91 2.453870305592739E-4 92-93 6.134675763981847E-5 94-95 2.453870305592739E-4 96-97 0.0017790559715547356 98-99 0.0036808054583891076 100-101 6.74814334038003E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 815039.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.045510990811266 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7447041993063 44.920318888697096 2 12.176561788912252 13.892363787193727 3 3.852418919456578 6.592896174332084 4 1.666798716403781 3.8033353798432805 5 0.9024288179419033 2.5739756526164834 6 0.5457616647355013 1.8679951826419496 7 0.3662388954963872 1.4624599456811216 8 0.27356131629304503 1.2484356060204558 9 0.19841229937079286 1.0186677904021828 >10 1.1473374994853183 12.089572852127597 >50 0.08701004999224411 3.383171300834964 >100 0.03575071229572413 3.7712026618224876 >500 0.0015075601550852006 0.6038396230463298 >1k 0.0010768286822037147 1.118976864029699 >5k 4.307314728814859E-4 1.652788290710534 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7653 0.9389734724350615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5800 0.7116223886218942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4106 0.5037795737381892 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1405 0.17238438896788988 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1336 0.16391853641359494 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1243 0.1525080394925887 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1018 0.12490199855467038 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 998 0.12244812824907765 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 952 0.11680422654621436 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.2269351527963694E-4 0.0 0.0 0.0 3 0.0 1.2269351527963694E-4 0.0 0.0 0.0 4 0.0 1.2269351527963694E-4 0.0 0.0 0.0 5 0.0 1.2269351527963694E-4 0.0 0.0 0.0 6 2.453870305592739E-4 1.2269351527963694E-4 0.0 0.0 0.0 7 2.453870305592739E-4 1.2269351527963694E-4 0.0 0.0 0.0 8 2.453870305592739E-4 1.2269351527963694E-4 0.0 0.0 0.0 9 2.453870305592739E-4 1.2269351527963694E-4 0.0 0.0 0.0 10-11 2.453870305592739E-4 1.2269351527963694E-4 0.0 0.0 0.0 12-13 2.453870305592739E-4 1.2269351527963694E-4 0.0 0.0 0.0 14-15 2.453870305592739E-4 1.2269351527963694E-4 0.0 6.134675763981847E-5 0.0 16-17 2.453870305592739E-4 1.2269351527963694E-4 0.0 1.2269351527963694E-4 0.0 18-19 4.2942730347872925E-4 1.2269351527963694E-4 0.0 1.2269351527963694E-4 0.0 20-21 4.907740611185478E-4 1.2269351527963694E-4 0.0 1.840402729194554E-4 0.0 22-23 4.907740611185478E-4 1.2269351527963694E-4 0.0 7.361610916778215E-4 0.0 24-25 4.907740611185478E-4 1.2269351527963694E-4 0.0 8.588546069574585E-4 0.0 26-27 4.907740611185478E-4 1.2269351527963694E-4 0.0 0.0013496286680760063 0.0 28-29 5.521208187583661E-4 1.2269351527963694E-4 0.0 0.0025765638208723753 0.0 30-31 6.134675763981846E-4 1.2269351527963694E-4 0.0 0.0046623535806262035 0.0 32-33 6.134675763981846E-4 1.2269351527963694E-4 0.0 0.006686796582740212 0.0 34-35 6.748143340380031E-4 1.2269351527963694E-4 0.0 0.009079320130693132 0.0 36-37 7.361610916778215E-4 1.2269351527963694E-4 0.0 0.011901270982124783 0.0 38-39 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.015029955621755523 0.0 40-41 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.018956148110703903 0.0 42-43 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.028342202029596128 0.0 44-45 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.04116367437631819 0.0 46-47 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.05527342863347644 0.0 48-49 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.08876875830481731 0.0 50-51 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.1193194436094469 0.0 52-53 8.588546069574585E-4 1.2269351527963694E-4 0.0 0.15299881355370726 0.0 54-55 8.588546069574585E-4 2.453870305592739E-4 0.0 0.21446826470880534 0.0 56-57 8.588546069574585E-4 2.453870305592739E-4 0.0 0.27182748310203564 0.0 58-59 8.588546069574585E-4 2.453870305592739E-4 0.0 0.3365483124120441 0.0 60-61 8.588546069574585E-4 2.453870305592739E-4 0.0 0.4164831376167275 0.0 62-63 9.815481222370955E-4 2.453870305592739E-4 0.0 0.5273980754295193 0.0 64-65 9.815481222370955E-4 2.453870305592739E-4 0.0 0.5962904842590355 0.0 66-67 9.815481222370955E-4 3.6808054583891076E-4 0.0 0.716039355171961 0.0 68-69 9.815481222370955E-4 3.6808054583891076E-4 0.0 0.8798351980702763 0.0 70-71 0.0011042416375167323 3.6808054583891076E-4 0.0 0.9747999788967154 0.0 72-73 0.0011042416375167323 3.6808054583891076E-4 0.0 1.1271239781163847 0.0 74-75 0.0011042416375167323 3.6808054583891076E-4 0.0 1.326684980718714 0.0 76-77 0.0011042416375167323 3.6808054583891076E-4 0.0 1.4614638072533952 0.0 78-79 0.0011042416375167323 3.6808054583891076E-4 0.0 1.5837892419871933 0.0 80-81 0.0011042416375167323 3.6808054583891076E-4 0.0 1.7841477524388405 0.0 82-83 0.0011042416375167323 3.6808054583891076E-4 0.0 2.0321113468189864 0.0 84-85 0.0011042416375167323 3.6808054583891076E-4 0.0 2.327066557551234 0.0 86-87 0.0011042416375167323 3.6808054583891076E-4 0.0 2.76428489925022 0.0 88-89 0.0011042416375167323 3.6808054583891076E-4 0.0 3.2175147446931986 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 55 0.0025798895 34.543537 6 GTATCAA 8850 0.0 31.245138 1 TATTCCG 95 3.4909346E-5 29.998333 5 CCGTTGC 95 3.4909346E-5 29.998333 9 GGTATCA 5910 0.0 28.780495 1 TATCAAC 10780 0.0 25.68735 2 ATCAACG 10795 0.0 25.607656 3 TCAACGC 10845 0.0 25.489594 4 CAACGCA 11005 0.0 25.119003 5 AACGCAG 11135 0.0 24.868399 6 CCTATAC 140 1.779422E-5 23.74868 3 GAGTACT 5850 0.0 23.708086 12-13 GTACATG 5700 0.0 23.505905 1 AGTACTT 5990 0.0 23.153973 12-13 ACGCAGA 12265 0.0 22.499771 7 TACATGG 5925 0.0 22.365847 2 CGCAGAG 12310 0.0 22.301767 8 GTACTTT 6225 0.0 22.089811 14-15 ATTCCGT 130 2.9415966E-4 21.92186 6 TCCGTTG 130 2.9415966E-4 21.92186 8 >>END_MODULE